Few reports have been seen on the segregation of polyploids and on the relationship between the segregation of SSR loci and that of the morphological traits in polyploid progeny. This paper attempted to gain an insigh...Few reports have been seen on the segregation of polyploids and on the relationship between the segregation of SSR loci and that of the morphological traits in polyploid progeny. This paper attempted to gain an insight into the segregation of the hexaploid tobacco progeny and to understand the correlation of the segregation of SSR loci with that of the flower morphological traits. The segregation was evaluated with both SSR markers and morphological traits of the flowers. Twenty pairs of SSR primers were screened and a total of 42 SSR loci were identified. Chi-square analysis showed that the segregation ratio of the SSR loci in the aneuploid progeny was between 1:1 and 2:1. Six morphological traits of flowers, including the lengths of the flower and the calyx, the widths of the calyx, the corolla tube and the corolla, and the coloring of the flower, from 180 progeny and 2 progenitor plants, were determined and Chi-square analyzed. All traits were in consistent also with a segregation ratio of 1:1 to 2:1 except the width of the calyx.展开更多
【目的】探讨CfFT基因在蕙兰成花中的作用。【方法】采用RT-PCR结合RACE技术从蕙兰(Cymbidiumfaberi)中克隆Flowering locus T(FT)同源基因CfFT。采用实时定量RT-PCR对不同组织及不同花发育时期CfFT进行表达分析。将该基因克隆到PBI121...【目的】探讨CfFT基因在蕙兰成花中的作用。【方法】采用RT-PCR结合RACE技术从蕙兰(Cymbidiumfaberi)中克隆Flowering locus T(FT)同源基因CfFT。采用实时定量RT-PCR对不同组织及不同花发育时期CfFT进行表达分析。将该基因克隆到PBI121载体上导入烟草中,并对不同转基因烟草株系中的CfFT、NFL、NtFUL和NAP1基因进行实时定量RT-PCR分析。【结果】对蕙兰花芽分化不同时期的CfFT的表达分析表明,CfFT在花芽分化初期的表达量最高,之后随着花芽的成熟表达量逐渐降低。将CfFT导入烟草进行异源表达,转基因株系表现出明显的早花表型。对开花时间不一的转基因株系中的CfFT表达分析表明,其表达量与转基因烟草开花时间早晚成正比。进一步对这些株系内源的NFL、NAP1和NtFUL表达分析表明,NFL、NAP1和NtFUL基因的表达量与CfFT表达成正比,说明NFL、NAP1和NtFUL的表达受FT基因的上游调控。【结论】在烟草中异源表达蕙兰中的CfFT基因能促进烟草提前开花。展开更多
[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating t...[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating the flowering mechanism in poplar. [ Method] Plant expression vectors of AP1 genes were constructed and transformed into tobacco leaf disks with Agrobacterium-mediated method. Transgenic tobacco plants were identified by PCR. [ Result] AP1 genes were integrated into the genome of tobacco. Transgenic tobacco plants all presented an early flowering phenotype compared with wild-type tobacco. [ Conclusion] AP1 genes could promote early flowering in transgenic tobacco plants, which provided theoretical basis for molecular regulation of flowering in poplar.展开更多
文摘Few reports have been seen on the segregation of polyploids and on the relationship between the segregation of SSR loci and that of the morphological traits in polyploid progeny. This paper attempted to gain an insight into the segregation of the hexaploid tobacco progeny and to understand the correlation of the segregation of SSR loci with that of the flower morphological traits. The segregation was evaluated with both SSR markers and morphological traits of the flowers. Twenty pairs of SSR primers were screened and a total of 42 SSR loci were identified. Chi-square analysis showed that the segregation ratio of the SSR loci in the aneuploid progeny was between 1:1 and 2:1. Six morphological traits of flowers, including the lengths of the flower and the calyx, the widths of the calyx, the corolla tube and the corolla, and the coloring of the flower, from 180 progeny and 2 progenitor plants, were determined and Chi-square analyzed. All traits were in consistent also with a segregation ratio of 1:1 to 2:1 except the width of the calyx.
文摘【目的】探讨CfFT基因在蕙兰成花中的作用。【方法】采用RT-PCR结合RACE技术从蕙兰(Cymbidiumfaberi)中克隆Flowering locus T(FT)同源基因CfFT。采用实时定量RT-PCR对不同组织及不同花发育时期CfFT进行表达分析。将该基因克隆到PBI121载体上导入烟草中,并对不同转基因烟草株系中的CfFT、NFL、NtFUL和NAP1基因进行实时定量RT-PCR分析。【结果】对蕙兰花芽分化不同时期的CfFT的表达分析表明,CfFT在花芽分化初期的表达量最高,之后随着花芽的成熟表达量逐渐降低。将CfFT导入烟草进行异源表达,转基因株系表现出明显的早花表型。对开花时间不一的转基因株系中的CfFT表达分析表明,其表达量与转基因烟草开花时间早晚成正比。进一步对这些株系内源的NFL、NAP1和NtFUL表达分析表明,NFL、NAP1和NtFUL基因的表达量与CfFT表达成正比,说明NFL、NAP1和NtFUL的表达受FT基因的上游调控。【结论】在烟草中异源表达蕙兰中的CfFT基因能促进烟草提前开花。
基金Supported by National Natural Science Foundation of China(31370661)
文摘[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating the flowering mechanism in poplar. [ Method] Plant expression vectors of AP1 genes were constructed and transformed into tobacco leaf disks with Agrobacterium-mediated method. Transgenic tobacco plants were identified by PCR. [ Result] AP1 genes were integrated into the genome of tobacco. Transgenic tobacco plants all presented an early flowering phenotype compared with wild-type tobacco. [ Conclusion] AP1 genes could promote early flowering in transgenic tobacco plants, which provided theoretical basis for molecular regulation of flowering in poplar.
