A whitening toothpaste containing pyrrolidone groups was developed.Through the stain-removal experiment on the hydroxyapatite discs with tobacco stains,the stain-removal abilities of ammonium acryloyldimethyltaurate/V...A whitening toothpaste containing pyrrolidone groups was developed.Through the stain-removal experiment on the hydroxyapatite discs with tobacco stains,the stain-removal abilities of ammonium acryloyldimethyltaurate/VP copolymer and its toothpaste were studied.The results showed that the new toothpaste containing pyrrolidone groups had good whitening effect.The new whitening toothpaste had good safety and it had no damage to the soft and hard tissues.展开更多
Aim To analyse the cytomorphological features of kerati- nocytes in smears obtained from the oral mucosa of tobacco users and from oral squamous cell carcinoma lesions. Methodology Oral smears were obtained from clini...Aim To analyse the cytomorphological features of kerati- nocytes in smears obtained from the oral mucosa of tobacco users and from oral squamous cell carcinoma lesions. Methodology Oral smears were obtained from clinically, normal appearing mucosa of oral squamous cell carcinoma patients （n=20） and from the mucosa of smokers （n=20）, and apparently healthy individuals （n=20） were used as controls. The smears were histochemically stained and cytomorphological assessment of the keratinocytes was carried out. One-way ANOVA （Analysis of Variance） was used for comparing the parameters among multiple groups and Tukey-HSD test was used to compare the mean values between groups. Results The mean nuclear area of keratinocytes from the mucosa of tobacco users was 46 ± 2.57 and that of the oral squamous cell carcinoma lesion was 81.54±4.31. While there was a significant （P=0.001） reduction in the cellular area of keratinocytes from oral squamous cell carcinoma lesion when compared with those from oral smears of tobacco users. Conclusion Cytomorphometric analysis of keratinocytes can serve as a useful adjunct in the early diagnosis of oral squamous cell carcinomas.展开更多
【目的】克隆大豆豆荚特异性启动子,并对其进行功能分析,为研究抗病虫基因在大豆荚中的特异性表达奠定基础。【方法】利用PCR技术克隆得到豆荚特异性启动子(Pod Specific Promoter,PSP)的核心序列,用PSP序列取代质粒pBI121中的CaMV 35S...【目的】克隆大豆豆荚特异性启动子,并对其进行功能分析,为研究抗病虫基因在大豆荚中的特异性表达奠定基础。【方法】利用PCR技术克隆得到豆荚特异性启动子(Pod Specific Promoter,PSP)的核心序列,用PSP序列取代质粒pBI121中的CaMV 35S启动子,构建与GUS基因融合的豆荚特异性报告载体pPSP-GUS,通过农杆菌介导法转化烟草"NC89",对转基因植株进行分子生物学检测,将鉴定为阳性的植株经GUS组织化学染色,验证PSP片段的功能。【结果】所获得的豆荚特异性启动子PSP大小为1 270bp,与已报道序列的同源性为98%,具有多种典型的启动子表达调控元件,如A/T-rich core、CAATBOX、TATABOX、GATABOX等。将pPSP-GUS质粒转化烟草后,经PCR和Southern blot检测结果显示,成功获得了转基因阳性烟草植株。GUS活性检测结果显示,在转pPSPGUS质粒的烟草根和叶片中均未检测到GUS活性,在萼片中可检测到低水平的GUS活性;而在花荚中可检测到高水平的GUS活性,且明显高于转pBI121质粒的对照植株。【结论】克隆得到的豆荚特异性启动子PSP片段具有启动基因在豆荚中特异性表达的功能。展开更多
文摘A whitening toothpaste containing pyrrolidone groups was developed.Through the stain-removal experiment on the hydroxyapatite discs with tobacco stains,the stain-removal abilities of ammonium acryloyldimethyltaurate/VP copolymer and its toothpaste were studied.The results showed that the new toothpaste containing pyrrolidone groups had good whitening effect.The new whitening toothpaste had good safety and it had no damage to the soft and hard tissues.
文摘Aim To analyse the cytomorphological features of kerati- nocytes in smears obtained from the oral mucosa of tobacco users and from oral squamous cell carcinoma lesions. Methodology Oral smears were obtained from clinically, normal appearing mucosa of oral squamous cell carcinoma patients （n=20） and from the mucosa of smokers （n=20）, and apparently healthy individuals （n=20） were used as controls. The smears were histochemically stained and cytomorphological assessment of the keratinocytes was carried out. One-way ANOVA （Analysis of Variance） was used for comparing the parameters among multiple groups and Tukey-HSD test was used to compare the mean values between groups. Results The mean nuclear area of keratinocytes from the mucosa of tobacco users was 46 ± 2.57 and that of the oral squamous cell carcinoma lesion was 81.54±4.31. While there was a significant （P=0.001） reduction in the cellular area of keratinocytes from oral squamous cell carcinoma lesion when compared with those from oral smears of tobacco users. Conclusion Cytomorphometric analysis of keratinocytes can serve as a useful adjunct in the early diagnosis of oral squamous cell carcinomas.
