Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of th...Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of the correspondingisoenzymes.Nevertheless,we found in studyingthat the RSS changed with different staining展开更多
EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong diff...EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong different stain recipes for Est of 3 kinds of fungi-Lentinus edodes. Pleurotus sapidus. Phellinus igriarius and 2 kinds of plants-Populus sp and Brassica chinensis. Of the four kinds of Est staining recipes tested.the recipe α-acetic acid-naphther showed the best effect.and followed by β-aceticacid-naphther, semicontent α-aceticacid-naphther and α+β-aceticacidnathpher.展开更多
Copper is an important ingredient in fungicides,which are used for many economic plants.However,there is concern about the side-effects of copper-based fungicides due to their potential to affect beneficial mites.The ...Copper is an important ingredient in fungicides,which are used for many economic plants.However,there is concern about the side-effects of copper-based fungicides due to their potential to affect beneficial mites.The purpose of the present paper was to investigate the effects of copper on the development of the fruit fly,Drosophila melanogaster.It was found that higher doses of copper significantly prolonged the developmental time of the fruit flies,especially during the larval stages.When 320 mg L-1 copper-contaminated food was used,most of the larvae died when they were small and before the pupal stage.The protein contents and esterase isozymes extracted from the larvae changed according to the doses of copper.The small-molecular-weight protein bands gradually became weaker or were lost as the copper levels increased.However,low doses of copper stimulated a stronger expression of a few proteins.These results indicate that low doses of copper generally have no lethal effects on D.melanogaster because a specific group of genes,which encode specific proteins,are probably activated in order to withstand the onslaught of stressful conditions.At high doses of copper in food(e.g.320 mg L-1),fly development and viability are significantly affected.展开更多
文摘Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of the correspondingisoenzymes.Nevertheless,we found in studyingthat the RSS changed with different staining
文摘EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong different stain recipes for Est of 3 kinds of fungi-Lentinus edodes. Pleurotus sapidus. Phellinus igriarius and 2 kinds of plants-Populus sp and Brassica chinensis. Of the four kinds of Est staining recipes tested.the recipe α-acetic acid-naphther showed the best effect.and followed by β-aceticacid-naphther, semicontent α-aceticacid-naphther and α+β-aceticacidnathpher.
基金supported by the National Natural Science foundation of China (No.30300035)by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry[No. (2004)527].
文摘Copper is an important ingredient in fungicides,which are used for many economic plants.However,there is concern about the side-effects of copper-based fungicides due to their potential to affect beneficial mites.The purpose of the present paper was to investigate the effects of copper on the development of the fruit fly,Drosophila melanogaster.It was found that higher doses of copper significantly prolonged the developmental time of the fruit flies,especially during the larval stages.When 320 mg L-1 copper-contaminated food was used,most of the larvae died when they were small and before the pupal stage.The protein contents and esterase isozymes extracted from the larvae changed according to the doses of copper.The small-molecular-weight protein bands gradually became weaker or were lost as the copper levels increased.However,low doses of copper stimulated a stronger expression of a few proteins.These results indicate that low doses of copper generally have no lethal effects on D.melanogaster because a specific group of genes,which encode specific proteins,are probably activated in order to withstand the onslaught of stressful conditions.At high doses of copper in food(e.g.320 mg L-1),fly development and viability are significantly affected.
