Effects of Different Planting Years on Physicochemical Properties and Enzyme Activities in Soil of Rice-Cherry Tomato Rotation

Crop rotation periodicity has always been one of the research focuses currently. In this study, the physicochemical properties, nutrient contents and enzyme activities were investigated in soils from rice-cherry tomato rotation for one year (1a), three years (3a), five years (5a), seven years (7a) and ten years (10a), respectively. The major objective was to analyze the optimal rotation years of rice-cherry tomato from soil perspective, so as to provide theoretical basis for effectively avoiding continuous cropping obstacles of cherry tomato via studying the response characteristics of soil physicochemical properties, nutrient contents and enzyme activities to planting years of rice-cherry tomato rotation system. The results were as follows: 1) Soil pH value was increased year by year during 1a to 5a, reached the highest value 5.32 at 5a. However, soil acidity was sharply enhanced during 7a to 10a (P P •kg-1 at 5a. 3) The content of soil available phosphorus was increased year by year with increasing of crop rotation years, and increased by 110% to 173% during 3a to 10a (P P P < 0.05). In conclusion, long-term single rotation pattern of rice-cherry tomato would aggravate soil acidification, prompt soil nutrient imbalance and reduce soil enzyme activity. 5a to 7a would be the appropriate rotation period for rice-cherry tomato, or else it would reduce soil quality, resulting in a new continuous cropping obstacle of cherry tomato.

A Brief Study on pH, Exchangeable Ca²⁺and Mg²⁺in Farmlands under Tobacco-Rice Rotation in Xuancheng City of South Anhui

pH, exchangeable Ca2+ and Mg2+ of soil can influence the yield and quality of flue-cured tobacco. Xuancheng city is the dominant tobacco-planting region in Anhui province since 2008. A general soil survey was conducted in Xuancheng city to understand the current situations of pH, exchangeable Ca2+ and Mg2+ of farmlands under tobacco-rice rotation and to decide whether continuous applying dolomite powders to abate soil acidification. In total 124 topsoil samples (0-20 cm) were collected from the typical farmlands and soil pH, exchangeable Ca2+ and Mg2+ were measured. The results showed that soil pH and Ca2+ and Mg2+ in Xuancheng city were generally in the suitable grades, pH ranged from 4.56 to 8.42 with an average of 5.87, exchangeable Ca2+ ranged from 1.01 cmol(1/2Ca2+) kg-1 to 100.55 cmol(1/2Ca2+) kg-1 with an average of 11.07 cmol(1/2Ca2+) kg-1, and exchangeable Mg2+ ranged from 0.14 cmol(1/2Mg2+) kg-1 to 2.86 cmol(1/2Mg2+) kg-1 with an average of 0.81 cmol(1/2Mg2+) kg-1, all spanned from the low grades to the high grades. To the whole Xuancheng city, 37.10%, 62.10% and 72.58% of the surveyed farmlands were in the low grades of pH, exchangeable Ca2+ and Mg2+, respectively, while 52.42%, 25.00% and 20.97% of the farmlands were in the suitable grades, respectively. pH had an extremely significantly positive Napierian logarithm correlation with exchangeable Ca2+ and an extremely significantly positive power correlation with exchangeable Mg2+. In total about 338.2 kg·hm-2 of Ca2+ and 202.4 kg·hm-2 of Mg2+ are annually input into the farmlands, while about 110.4 kg·hm-2 of Ca2+ and 7.4 kg·hm-2 of Mg2+ are annually moved out from the farmlands, exchangeable Ca2+ and Mg2+ were annually increased by 225.2 kg·hm-2 and 187.6 kg·hm-2 in the farmlands, respectively. It is necessary to monitor the farmlands dynamically in order to decide whether continuous applying dolomite powders which should be dependent upon the specific conditions of individual farmlands.

Soil Fertility Comparison between Rotations of Tobacco-Rice (TR) and Rape-Rice (RR)

Tobacco-planting plays an important role in ensuring the high-quality tobacco raw materials supply and the local social and economic development in Chenzhou City. In recent years whether tobacco-planting is better for the maintenance and improvement of soil fertility than other crop-planting has been highly concerned. In this study, 16 soil fertility indicators and soil integrated index (IFI) were compared by 21 pairs of fields in Chenzhou city under the rotations of tobacco-rice (TF) and rice-rice (RR), and results showed that, comparing the mean values of soil fertility indicators, the contents of OM, TN, AN, AK, S and IFI were extremely significantly higher in TR than those in RR (p < 0.01), the contents of Cu, Ca, Mg and Fe were significantly higher in TR than those in RR (p < 0.05), but Mn content was significantly lower in TR than those in RR (p < 0.05). Meanwhile the contents of TP, TK and AP were insignificantly higher in TR than those in RR, and the contents of B, Mo and Zn were insignificantly lower in TR than those in RR. The above significant differences in soil fertility indicators were mainly due to relatively higher fertilizer inputs and less nutrient removal during tobacco-growing season than during rape-growing season, the net increase of N, P2O5 and K2O are 8.61, 5.25 and 24.89 kg per 667 m2 respectively in tobacco-growing season, while the net decrease of N, P2O5 and K2O are 8.88, 4.70 and 4.62 kg per 667 m2 respectively in rape-growing season. C.V. of soil fertility indicators and IFI were meanly lower in TR (52.25% and 15.95%, respectively) than those in RR (63.07% and 22.12%, respectively). Comparatively, tobacco-planting can improve soil fertility better than rape-planting when rotated with late rice in Chenzhou city. For tobacco-planting, Mg fertilizer should be applied for 23.8% TR fields, while more N, K, Ca, Mg, S and B fertilizers should be applied for 42.86%, 23.81%, 14.29%, 47.62%, 80.95% and 47.62% RR fields, respectively.

Effect of various crop rotations on rice yield and nitrogen use efficiency in paddy–upland systems in southeastern China

To evaluate the effects of various rotation systems on rice grain yield and N use efficiency, a paddy–upland cropping experiment(2013–2016) was conducted in southeastern China. The experiment was designed using six different rice––winter crop rotations: rice–fallow(RF),rice–wheat(RW), rice–potato with rice straw mulch(RP), rice–green manure(Chinese milk vetch; RC–G), rice–oilseed rape(RO), and rice–green manure crop(oilseed rape with fresh straw incorporated into soil at flowering; RO–G) and three N rates, N0(0 kg N ha-1), N1(142.5 kg N ha-1), and N2(202.5 kg N ha-1). Average rice yields in the RF(5.93 t ha-1) rotation were significantly lower than those in the rotations with winter crops(7.20–7.48 t ha-1)under the N0 treatment, suggesting that incorporation of straw might be more effective for increasing soil N than winter fallow. The rice yield differences among the rotations varied by year with the N input. In general, the grain yields in the RP and RO–G rotations –were respectively 11.6–28.5% and 14.80–37.19% higher than those in the RF in plots with N applied. Increasing the N rate may have tended to minimize the average yield gap between the RF and the other rotations; the yield gaps were 18.55%, 4.14%, and 0.23% in N0, N1, and N2, respectively. However, the N recovery efficiency in the RF was significantly lower than that in other rotations, except for 2015 under both N1 and N2 rates, a finding that implies a large amount of chemical N loss. No significant differences in nitrogen agronomic efficiency(NAE) and physiological efficiency(NPE) were found between the rotations with legume(RC–G) and non–legume(RO and RW) winter crops, a result that may be due partly to straw incorporation. For this reason, we concluded that the return of straw could reduce differences in N use efficiency between rotations with and without legume crops. The degree of synchrony between the crop N demand and the N supply was evaluated by comparison of nitrogen balance degree(NBD) values. The NBD values in the RP and RW were significantly lower than those in the other rotations under both N1 and N2 rates. Thus,in view of the higher grain yield in the RP compared to the RW under the N1 rate, the RP rotation might be a promising practice with comparable grain yield and greater N use efficiency under reduced N input relative to the other rotations. The primary yield components of the RF and RP were identified as number of panicles m-2 and numbers of kernels panicle-1, respectively. The NAE and NPE were positively correlated with harvest index, possibly providing a useful indicator for evaluating N use efficiency.

Cover Crops as Affecting Soil Chemical and Physical Properties and Development of Upland Rice and Soybean Cultivated in Rotation

Cover crops can provide changes in soil chemical and physical properties, which could allow a sustainable development of soybean and upland rice rotation in Brazilian Cerrado. The objective of this study was to determine the effects of cover crops(cultivated in the offseason) in the soybean-upland rice rotation(cultivated in the summer season) on the soil chemical and physical properties, yield components and grain yield of the cash crops. The experimental design was a randomized block design in factorial scheme 4 × 2 with six replications. Treatments were composed by four cover crops: fallow, millet(Pennisetum glaucum) + Crotalaria ochroleuca, millet + pigeon pea(Cajanus cajans), and millet + pigeon pea + Urochola ruziziensis in the offseason with one or two cycles of cover crops, with rice(Oryza sativa)or soybean(Glycine max) in the summer season. Cover crops alone provided no changes in soil chemical properties. However, the rotation cover crops/cash crops/cover crops/cash crops reduced p H, Al and H + Al and increased Ca, Mg, K and Fe contents in the soil. The cover crops millet + pigeon pea and millet + pigeon pea + U. ruziziensis improved soil physical properties in relation to fallow,especially in the 0–0.10 m soil layer. In spite of the improvement of the soil physical properties after two years of rotation with cover crops and cash crops, the soil physical quality was still below the recommended level, showing values of macroporosity, S index and soil aeration capacity lower than 0.10 m3/m3, 0.035 and 0.34, respectively. Upland rice production was higher under mixtures of cover crops than under fallow, mainly because of soil physical changes done by these mixtures of cover crops.Soybean grain yield was similar under all cover crops tested, but was higher after the rotation cover crops/upland rice/cover crops than after only one cycle of cover crops.

Effects of rice or wheat residue retention on the quality of milled japonica rice in a rice–wheat rotation system in China

In rice–wheat rotation systems, crop straw is usually retained in the field at land preparation in every, or every other, season. We conducted a 3-year-6-season experiment in the middle–lower Yangtze River Valley to compare the grain qualities of rice under straw retained after single or double seasons per year. Four treatments were designed as: both wheat and rice straw retained(WR), only rice straw retained(R), only wheat straw retained(W), and no straw retained(CK). The varieties were Yangmai 16 wheat and Wuyunjing 23 japonica rice. The results showed contrasting effects of W and R on rice quality. Amylopectin content, peak viscosity, cool viscosity, and breakdown viscosity of rice grain were significantly increased in W compared to the CK, whereas gelatinization temperature,setback viscosity, and protein content significantly decreased. In addition, the effect of WR on rice grain quality was similar to that of W, although soil fertility was enhanced in WR due to straw being retained in two cycles. The differences in protein and starch contents among the treatments might result from soil nitrogen supply. These results indicate that wheat straw retained in the field is more important for high rice quality than rice straw return, and straw from both seasons is recommended for positive effects on soil fertility.

Long-Term No-Tillage Direct Seeding Mode for Water-Saving and Drought-Resistance Rice Production in Rice-Rapeseed Rotation System

To study the effects of long-term no-tillage direct seeding mode on rice yield and the soil physiochemical property in a rice-rapeseed rotation system, a comparative experiment with a water-saving and drought-resistance rice （WDR） variety and a double low rapeseed variety as materials was conducted under no-tillage direct seeding （NTDS） mode and conventional tillage direct seeding （CTDS） mode for four years, using the CTDS mode as the control. Compared with the CTDS mode, the actual rice yield of WDR decreased by 8.10% at the first year, whereas the plant height, spikelet number per panicle, spikelet fertility, 1000-grain weight, grain yield, actual yield, and harvest index increased with no-tillage years, which led to the actual yield increase by 6.49% at the fourth year. Correlation analysis showed that the panicle length was significantly related to the actual yield of WDR. Compared with the CTDS mode in terms of the soil properties, the pH value of the NTDS mode decreased every year, whereas the contents of soil organic matter and total N of the NTDS mode increased. In the 0-5 cm layer of the NTDS mode, the soil bulk decreased, whereas the contents of soil organic matter, total N, and available N increased. In the 5-20 cm layer of the NTDS mode, the available N and K decreased, whereas the soil bulk, contents of soil organic matter, and total N increased. In summary, the NTDS mode increased the rice yield, and could improve the paddy soil fertility of the top layer.

Carbon Dioxide, Methane, and Nitrous Oxide Emissions from a Rice-Wheat Rotation as Affected by Crop Residue Incorporation and Temperature

Field measurements were made from June 2001 to May 2002 to evaluate the e?ect of crop residue application and temperature on CO2, CH4, and N2O emissions within an entire rice-wheat rotation season. Rapeseed cake and wheat straw were incorporated into the soil at a rate of 2.25 t hm?2 when the rice crop was transplanted in June 2001. Compared with the control, the incorporation of rapeseed cake enhanced the emissions of CO2, CH4, and N2O in the rice-growing season by 12.3%, 252.3%, and 17.5%, respectively, while no further e?ect was held on the emissions of CO2 and N2O in the following wheat- growing season. The incorporation of wheat straw enhanced the emissions of CO2 and CH4 by 7.1% and 249.6%, respectively, but reduced the N2O emission by 18.8% in the rice-growing season. Signi?cant reductions of 17.8% for the CO2 and of 12.9% for the N2O emission were observed in the following wheat- growing season. A positive correlation existed between the emissions of N2O and CO2 (R2 = 0.445,n = 73,p < 0.001) from the rice-growing season when N2O was emitted. A trade-o? relationship between the emissions of CH4 and N2O was found in the rice-growing season. The CH4 emission was signi?cantly correlated with the CO2 emission for the period from rice transplantation to ?eld drainage, but not for the entire rice-growing season. In addition, air temperature was found to regulate the CO2 emissions from the non-waterlogged period over the entire rice-wheat rotation season and the N2O emissions from the non- waterlogged period of the rice-growing season, which can be quantitatively described by an exponential function. The temperature coe?cient (Q10) was then evaluated to be 2.3±0.2 for the CO2 emission and 3.9±0.4 for the N2O emission, respectively.

Estimating daily actual evapotranspiration of a rice–wheat rotation system in typical farmland in the Huai River Basin using a two-step model and two one-step models

The objective of this study is to evaluate the performance of three models for estimating daily evapotranspiration(ET) by employing flux observation data from three years(2007, 2008 and 2009) during the growing seasons of winter wheat and rice crops cultivated in a farmland ecosystem(Shouxian County) located in the Huai River Basin(HRB), China. The first model is a two-step model(PM-Kc);the other two are one-step models(e.g., Rana-Katerji(R-K) and advection-aridity(AA)). The results showed that the energy closure degrees of eddy covariance(EC) data during winter wheat and rice-growing seasons were reasonable in the HRB, with values ranging from 0.84 to 0.91 and R2 of approximately 0.80. Daily ET of winter wheat showed a slow decreasing trend followed by a rapid increase, while that of rice presented a decreasing trend after an increase. After calibrating the crop coefficient(Kc), the PM–Kc model performed better than the model using the Kc recommended by the Food and Agricultural Organization(FAO). The calibrated key parameters of the R-K model and AA model showed better universality. After calibration, the simulation performance of the PM-Kc model was satisfactory. Both the R-K model and AA model underestimated the daily ET of winter wheat and rice. Compared with that of the R-K model, the simulation result of the AA model was better, especially in the simulation of daily ET of rice. Overall, this research highlighted the consistency of the PM-Kc model to estimate the water demand for rice and wheat crops in the HRB and in similar climatic regions in the world.

Seasonal and Interannual Variations of Carbon Exchange over a Rice–Wheat Rotation System on the North China Plain

Rice-wheat （R-W） rotation systems are ubiquitous in South and East Asia, and play an important role in modulating the carbon cycle and climate. Long-term, continuous flux measurements help in better understanding the seasonal and interannual variation of the carbon budget over R-W rotation systems. In this study, measurements of CO2 fluxes and meteorological variables over an R-W rotation system on the North China Plain from 2007 to 2010 were analyzed. To analyze the abiotic factors regulating Net Ecosystem Exchange （NEE）, NEE was partitioned into gross primary production （GPP） and ecosystem respiration. Nighttime NEE or ecosystem respiration was controlled primarily by soil temperature, while daytime NEE was mainly determined by photosythetically active radiation （PAR）. The responses of nighttime NEE to soil temperature and daytime NEE to light were closely associated with crop development and photosynthetic activity, respectively. Moreover, the interannual variation in GPP and NEE mainly depended on precipitation and PAR. Overall, NEE was negative on the annual scale and the rotation system behaved as a carbon sink of 982 g C m 2 per year over the three years. The winter wheat field took up more CO2 than the rice paddy during the longer growing season, while the daily NEE for wheat and rice were -2.35 and -3.96 g C m-2, respectively. After the grain harvest was subtracted from the NEE, the winter wheat field became a moderately strong carbon sink of 251-334 g C m-2 per season, whereas the rice paddy switched to a weak carbon sink of 107-132 per season.

A Field Study on Effects of Nitrogen Fertilization Modes on Nutrient Uptake,Crop Yield and Soil Biological Properties in Rice-Wheat Rotation System

Rational application of nitrogen （N） fertilizers is an important measure to raise N fertilizer recovery rate and reduce N loss.A two-year field experiment of rice-wheat rotation was employed to study the effects of N fertilization modes including a N fertilizer reduction and an organic manure replacement on crop yield,nutrient uptake,soil enzyme activity,and number of microbes as well as diversity of microbes.The result showed that 20% reduction of traditional N fertilizer dose of local farmers did not significantly change crop yield,N uptake,soil enzyme activity,and the number of microbes （bacteria,actinomycetes,and fungi）.On the basis of 20% reduction of N fertilizer,50% replacement of N fertilizer by organic manure increased the activity of sucrose,protease,urease,and phosphatase by 46-62,27-89,33-46,and 35-74%,respectively,and the number of microbes,i.e.,bacteria,actinomycetes,and fungi by 36-150,11-153,and 43-56%,respectively.Further,organic fertilizer replacement had a Shannon＇s diversity index （H） of 2.18,which was higher than that of other modes of single N fertilizer application.The results suggested that reducing N fertilizer by 20% and applying organic manure in the experimental areas could effectively lower the production costs and significantly improve soil fertility and biological properties.

A Process-based Model of N_2O Emission from a Rice-Winter Wheat Rotation Agro-Ecosystem:Structure,Validation and Sensitivity

In order to numerically simulate daily nitrous oxide （N2O） emission from a rice-winter wheat rotation cropping system, a process-based site model was developed （referred to as IAP-N-GAS） to track the movement and transformation of several forms of nitrogen in the agro-eeosystem, which is affected by climate, soil, crop growth and management practices. The simulation of daily N2O fluxes, along with key daily environmental variables, was validated with three-year observations conducted in East China. The validation demonstrated that the model simulated well daily solar radiation, soil temperature and moisture, and also captured the dynamics and magnitude of accumulated rice aboveground biomass and mineral nitrogen in the soil. The simulated daily N2O emissions over all three years investigated were generally in good agreement with field observations. Particularly well simulated were the peak N2O emissions induced by fertilizations, rainfall events or mid-season drainages. The model simulation also represented closely the inter-annuM variation in N2O emission. These validations imply that the model has the capability to capture the general characteristics of N2O emission from a typical rice-wheat rotation agro-ecosystem. Sensitivity analyses revealed that the simulated N2O emission is most sensitive to the fertilizer application rate and the soil organic matter content, but it is much less sensitive to variations in soil pH and texture, temperature, precipitation and crop residue incorporation rate under local conditions.

Study on the Characteristics and Efficient Cultivation Techniques of "Rice-Rice-Potato" Triple Cropping Rotation Pattern

The efficient cultivation technique of "rice-rice-potato" triple cropping rotation pattern is summarized by analyzing its characteristics and cultivation techniques in Yangjiang region of Guangdong Province,China,and machine-transplanted cultivation technique of double cropping rice and cultivation techniques of winter potato black film mulching are emphatically introduced,in order to promote the development of potato industry in Yangjiang City.The results will provide a scientific basis for the popularization and application of the "rice-rice-potato" triple cropping rotation pattern.

Yield Gap Analysis of Wheat in Rice-wheat Rotation Regions of Anhui Province,China

The present study was planned to analyze the yield gap of wheat and its production constraints in order to explore the approaches for narrowing the yield gap of wheat in different wheat-rice rotation regions of Anhui Province. The production status and limiting factors of wheat in three rice-wheat rotation regions which are named Region Ⅰ,Region Ⅱ and Region Ⅲ were surveyed by using participatory rural appraisal method. The personnel,who were engaged in wheat production in rice-wheat rotation regions of Anhui Province,mainly ageing from 41 to 60,accounted for 79% of the total personnel in the regions. There were significant differences in yield of wheat which was planted after rice in Anhui. The yield was ranging from 8 907. 00 to 2 700. 00 kg/ha from north to south with an average of 4 978. 5 kg/ha,and the rank of overall average yields at province level was Region Ⅰ( 5 685. 60 kg/ha) > Region Ⅱ( 5 600. 10 kg/ha) > Region Ⅲ( 3 048. 60 kg/ha). The average yield gap of wheat in wheat-rice rotation regions at province level was up to 2 637. 00 kg/ha,and the extreme yield gaps per hectare in the same region were 2 778. 00 kg( Region Ⅰ),2 502. 00 kg( Region Ⅱ) and 1 575. 00 kg( Region Ⅲ) respectively. The objective constraints were Fusarium head blight and pre-harvest sprouting;the subjective constraints were variety selection and layout,poor sowing quality and low seedling quality;social constraints were high cost,low market price and poor efficiency;and ecological constraints were poor soil texture,soil infertility and poor water-and-fertilizer retention. The yield gap of wheat in rice-wheat rotation regions can be effectively reduced by improving yield potential of low-and-medium-yielding fields. Selecting appropriate wheat varieties and layout,constructing disease forecast system,improving agricultural machinery and social service organizations of plant protection,and strengthening scientific training as well as technological training of new agricultural operators and agricultural machinery technicians are the core means to narrowing the yield gap of wheat in rice-wheat rotation regions at province scale.

Green manuring facilitates bacterial community dispersal across different compartments of subsequent tobacco

Green manure–crop rotation is a sustainable approach to protect crops against diseases and improve yield.However,the mechanism by which green manuring manipulates the crop-associated microbial community remains to be elucidated.In this study,we explored the horizontal processes of bacterial communities in different compartments of the soil–root interface(bulk soil,rhizosphere soil,rhizoplane and endosphere)of tobacco by performing a field experiment including four rotation practices,namely,tobacco rotated with smooth vetch,ryegrass,radish,and winter fallow(without green manure).Results showed that the co-occurrence networks constructed by adjacent compartments of the soil–root interface with green manuring had more edges than without green manuring,indicating dramatic microbial interactions.Green manuring increased the dispersal-niche continuum index between bulk soil and other compartments,indicating that it facilitated the horizontal dispersal of microbes.For the different green manuring practices,the neutral community model explained 24.6–27.6%of detection frequency for bacteria,and at least one compartment under each practice had a normalized stochasticity ratio higher than the 50%boundary point,suggesting that the deterministic and stochastic processes jointly shaped the tobacco microbiome.In conclusion,green manuring generally facilitates bacterial community dispersal across different compartments and enhances potential interactions among adjacent compartments.This study provides empirical evidence for understanding the microbiome assembly under green manure–crop rotation.

Increasing the appropriate seedling density for higher yield in dry direct-seeded rice sown by a multifunctional seeder after wheatstraw return

Dry direct-seeded rice(DDR) sown using a multifunctional seeder that performs synchronous rotary tillage and sowing has received increased attention because it is highly efficient,relatively cheap,and environmentally friendly.However,this method of rice production may produce lower yields in a rice–wheat rotation system because of its poor seedling establishment.To address this problem,we performed field experiments to determine the rice yield at five seedling density levels(B1,B2,B3,B4,and B5=100,190,280,370,and 460 seedlings m-2,respectively) and clarify the physiological basis of yield formation.We selected a representative high-quality rice variety and a multifunctional seeder that used in a typical rice–wheat rotation area in 2016 and 2018.The proportion of main stem panicle increased with increasing seedling density.There was a parabolic relationship between yield and seedling density,and the maximum yield(9.34-9.47 t ha-1) was obtained under B3.The maximum yield was associated with a higher total spikelet number m-2 and greater biomass accumulation from heading to maturity.The higher total spikelet number m-2 under B3 was attributed to an increase in panicle number m-2 compared with B1 and B2.Although the panicle numbers also increased under B4 and B5,these increases were insufficient to compensate for the reduced spikelet numbers per panicle.Lower biomass,smaller leaf area,and lower N uptake per plant from the stem elongation stage to the heading stage were partially responsible for the smaller panicle size at higher seedling density levels such as B5.The higher biomass accumulation under B3 was ascribed to the increases in the photosynthetic rate of the top three leaves m-2 of land,crop growth rate,net assimilation rate,and leaf area index.Furthermore,the B3 rice population was marked by a higher grain–leaf ratio,as well as a lower export ratio and transport ratio of biomass per stem-sheath.A quadratic function predicted that 260-290 seedlings m-2 is the optimum seedling density for achieving maximum yield.Together,these results suggested that appropriately increasing the seedling density,and thereby increasing the proportion of panicles formed by the main stem,is an effective approach for obtaining a higher yield in DDR sown using a multifunctional seeder in a rice–wheat rotation system.

Transformation of indica rice with plasmid pBG1121 containing a tobacco endo-chitinase gene Ⅰ

Several plasmids, which were suitable for cereals transformation, have been reported. In the study, rice was transformed by a new plasmid pBG1121 containing a tobacco endo-chitinase gene ( TchiB).

Influence of rotation system on siderophere-producing microorganism(SPM) in rhizosphere soil of southern China

Soil siderophores are important for crop growth,benefit ferric iron absorption of root,and are affected by cropping patterns.The objective of this study was to evaluate the quantity of siderophores in soil of 2 continuous crop rotation patterns over 30 years in Anren country,China.Quantity and siderophore-producing capability of microorganisms in rice-riceoilseed rape(DDY)rotation and rice-rice(DD)rontinuous cropping rhizosphere soil were tested and analyzed by chrome azurol S method.Isolated strains were used to identify siderophore-producing microorganism(SPM)by PCR amplification and DNA sequencing.Results showed that 9 siderophore-producing bacteria strains were isolated from DDY rhizosphere soil while 7 strains were identified from DD rhizosphere soil.The mean solubility index which representing siderophore-producing capability of strains was 3.05.PCR amplification results indicated that bacterial were the major SPM in soil.This research indicates that crop rotation systems could drive microorganisms to produce siderophores and enrich them in bacterial communities.

江汉平原典型种植模式稻田土壤中农药残留特征

在江汉平原中稻-油菜/小麦轮作田、一季中稻田及再生稻田3种种植模式稻田采集土壤样品86个。调查了土壤样品中滴滴涕(DDTs,包括o,p′-DDT、p,p′-DDT、p,p′-DDE、p,p′-DDD)、六六六(HCHs,包括α-HCH、β-HCH、γ-HCH、δ-HCH)、甲胺磷、甲基对硫磷、氧乐果、乐果、敌敌畏、乙酰甲胺磷、毒死蜱、氯虫苯甲酰胺、吡蚜酮、吡虫啉、啶虫脒、三环唑、戊唑醇、氰氟草酯、二氯喹啉酸、乙草胺及异丙甲草胺等25种农药及其代谢产物的残留。结果表明,检出率最高的3种农药为当前常用杀虫剂氯虫苯甲酰胺(87.21%),杀菌剂三环唑(91.86%)及戊唑醇(72.09%),其残留均值分别为0.0397、0.0734 mg/kg及0.0276 mg/kg;已经禁用的有机氯农药滴滴涕(o,p′-DDT、p,p′-DDT、p,p′-DDE、p,p′-DDD)仍能检出,检出率为36.05%~66.28%。3种模式田块中检出的滴滴涕主要来源于历史上滴滴涕的使用。杀虫剂吡蚜酮及杀菌剂三环唑在再生稻田的残留量均值显著高于中稻-油菜/小麦轮作田及一季中稻田;但其他农药在不同种植模式稻田中的残留没有显著差异。本研究为江汉平原农药残留的治理及农药的合理使用提供了参考信息。

广西水稻产区稻-菜轮作田水稻根结线虫病发生情况调查及病原鉴定

为了明确广西水稻产区稻-菜轮作田根结线虫发生情况及其病原分类地位,在广西14个市27个乡(镇)的171块水稻产区稻-菜轮作田进行了采样调查,并进行致病线虫分离,根据2龄幼虫形态、雌虫会阴花纹特征等,结合核糖体ITS区和28S rDNA D2D3区序列分析方法对病原线虫种类进行鉴定。结果表明,共有64个田块发生根结线虫为害,水稻种植季根结线虫检出率为37.4%,蔬菜轮作季根结线虫检出率为25.7%。广西水稻根结线虫病病原为拟禾本科根结线虫Meloidogyne graminicola。说明拟禾本科根结线虫病在广西稻-菜轮作田发生已经相当普遍,应当引起重视。