Investigation of Gray Mold of Protected Tomato and Resistance Identification of Variety in Shihezi Area,Xinjiang

[Objective] The paper was to understand the occurrence of gray mold of protected tomato and resistance of introduced tomato varieties to gray mold in Shihezi area. [Method] The occurrence status of gray mold in protected tomato in Shawan County, Manas County, the experimental field of Shihezi University and Shihezi Huayu base were investigated during the occurrence period of tomato gray mold, and the resistance of intro-duced variety against tomato gray mold was identified at seedling stage through indoor artificial inoculation method. [Result] The diseased leaf rate of tomato gray mold was 17.52%-100%, the diseased fruit rate was 3.1%-55.69%, and the disease index was 15.98-100. In resistance identifica-tion of different tomato varieties, the disease indexes of Kenfan25, Shifan28, Shifan15, KENFAN-3, KENFAN-5, Shifan35, KENFAN-2 and Shifan-9 were 1.03, 3.1, 1.17, 4.3, 5.32, 5.12, 4.32 and 4.11, respectively. From September to March of the following year, tomato gray mold occurred and was prevalent in greenhouses in Shihezi area. [Conclusion] Shifan28, Kenfan25 and Shifan15 showed resistance to tomato gray mold, while the other five varieties were susceptible to the disease.

Plant immune inducer ZNC promotes rutin accumulation and enhances resistance to Botrytis cinerea in tomato

Gray mold is a destructive disease caused by Botrytis cinerea,a pervasive plant pathogen,which poses a threat to both tomato growth and postharvest storage.The utilization of induced resistance presents a potential strategy for combating plant pathogenic attacks.ZNC(zhinengcong),an extract derived from the endophytic fungus Paecilo-myces variotii,has been discovered to play a vital role in preventing diverse forms of bacterial infections.Nevertheless,the precise mechanism behind its ability to enhance tomato resistance to fungi remains unclear.In this study,we found that the exogenous spraying of ZNC could significantly improve the resistance of tomato plants to B.cinerea.The results of both the metabolomic analysis and high-performance liquid chromatography(HPLC)demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of rutin.Additional transcriptome analysis uncovered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species(ROS)and phosphorylation of mitogen-activated protein kinases(MPKs)related genes expression during the initial phase of invasion by B.cinerea.In addition,we also found that rutin might activate plant immunity by eliciting ethylene(ET)and jasmonic acid(JA)-mediated pathways.Therefore,plant immune inducer ZNC and rutin has bright application prospects and high utilization value to control gray mold.