[Objective]The paper was to isolate and identify Botrytis cinerea from processing tomato.[Method]The strain nky007a was isolated and purified from fruits infected by tomato grey mold.After identification,it was determ...[Objective]The paper was to isolate and identify Botrytis cinerea from processing tomato.[Method]The strain nky007a was isolated and purified from fruits infected by tomato grey mold.After identification,it was determined as B.cinerea.The 18S rDNA and internal transcriptional spacer(ITS)sequences of strain nky007a were amplified for molecular identification and classification.[Result]The 18S rDNA sequence of the strain shared the homology of 99.95%with Botryotinia fuckeliana(EF 110887.1);the ITS rDNA sequence had the homology of 100%with Botryotinia fuckeliana(AB444949.1),and there was no difference in bases.Phylogenetic tree analysis of strain nky007a indicated that nky007a belonged to Botrytis.When healthy tomatoes were infected by the strain,the incidence rate of tomato grey mold was up to 95%,indicating the pathogenicity of the strain.[Conclusion]Morphological identification,molecular identification and pathogenicity analysis results show that the strain nky007a is a highly pathogenetic strain B.cinerea.展开更多
[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzi...[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzianum WDG,200 million spores/g Trichoderma WP,24% validamycin AS,0.3% matrine AS on grape gray mold.[Results]Of the 4 biopesticides,200 million spores/g Trichoderma WP had the highest control effect on grape gray mold,with the control effect of 73.38% at 7 d post the second application,significantly higher than those of 100 million CFU/g T.harzianum WG and 0.3% matrine AS;the control effect was 75.77% at 14 d post the second application,significantly higher than those of other 3 biopesticides.[Conclusions]The 200 million spores/g Trichoderma WP shows a good control effect on grape gray mold with a long-lasting control effect,so it is more is for the control of summer black grape gray mold in Jianshui County.展开更多
基金Science and Technology Guidance Program of Xinjiang Academy of Agricultural Reclamation Sciences(2020YJ019).
文摘[Objective]The paper was to isolate and identify Botrytis cinerea from processing tomato.[Method]The strain nky007a was isolated and purified from fruits infected by tomato grey mold.After identification,it was determined as B.cinerea.The 18S rDNA and internal transcriptional spacer(ITS)sequences of strain nky007a were amplified for molecular identification and classification.[Result]The 18S rDNA sequence of the strain shared the homology of 99.95%with Botryotinia fuckeliana(EF 110887.1);the ITS rDNA sequence had the homology of 100%with Botryotinia fuckeliana(AB444949.1),and there was no difference in bases.Phylogenetic tree analysis of strain nky007a indicated that nky007a belonged to Botrytis.When healthy tomatoes were infected by the strain,the incidence rate of tomato grey mold was up to 95%,indicating the pathogenicity of the strain.[Conclusion]Morphological identification,molecular identification and pathogenicity analysis results show that the strain nky007a is a highly pathogenetic strain B.cinerea.
基金Supported by Scientific and Technological Innovation Platform Construction Project for Biological Control Resources Exploration and Utilization in Yunnan Province(202207AB110009)。
文摘[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzianum WDG,200 million spores/g Trichoderma WP,24% validamycin AS,0.3% matrine AS on grape gray mold.[Results]Of the 4 biopesticides,200 million spores/g Trichoderma WP had the highest control effect on grape gray mold,with the control effect of 73.38% at 7 d post the second application,significantly higher than those of 100 million CFU/g T.harzianum WG and 0.3% matrine AS;the control effect was 75.77% at 14 d post the second application,significantly higher than those of other 3 biopesticides.[Conclusions]The 200 million spores/g Trichoderma WP shows a good control effect on grape gray mold with a long-lasting control effect,so it is more is for the control of summer black grape gray mold in Jianshui County.
