我国高粱上发生的新病害顶腐病是由亚粘团串珠镰孢(Fusarinm moniliforme var. subglutinans Wr.＆Reink.)侵染引起的。病菌生长的适宜温度为25～30℃,适宜pH值为6～8。小型分生孢子萌发的适宜温度为25～28℃,适宜pH值为6～7。病菌在Bi...我国高粱上发生的新病害顶腐病是由亚粘团串珠镰孢(Fusarinm moniliforme var. subglutinans Wr.＆Reink.)侵染引起的。病菌生长的适宜温度为25～30℃,适宜pH值为6～8。小型分生孢子萌发的适宜温度为25～28℃,适宜pH值为6～7。病菌在Bilai培养基上,pH4～11间均易产生大型分生孢子。黑光灯和日光灯照射对大型分生孢子形成有促进作用,且黑光灯优于日光灯;在光照处理下最适宜产生大型孢子的培养基为小麦粒和高粱粒。病菌在PSA培养基上培养10～12天后可产生厚垣孢子。病菌能利用多种碳源,以半乳糖、甘露糖为佳,乳糖、木糖、葡萄糖次之。对氮源利用远不及碳源。人工接种条件下,病菌能侵染高粱、苏丹草、哥伦布草、玉米、谷子、珍珠粟、薏苡、水稻、燕麦、小麦、狗尾草等禾本科植物。展开更多
Recently, a new bacterial top rot disease of maize has frequently appeared in many areas of Yunnan Province, China. The pathogen of the disease was identified as Klebsiella pneumoniae （KpC4）, which is well known to ...Recently, a new bacterial top rot disease of maize has frequently appeared in many areas of Yunnan Province, China. The pathogen of the disease was identified as Klebsiella pneumoniae （KpC4）, which is well known to cause pulmonary and urinary diseases in humans and animals and occasionally exists as a harmless endophyte in plants. To evaluate the viru- lence of the maize pathogen to maize and mice, we inoculated maize and mice with routine inoculation and intraperitoneal injection respectively according to Koch＇s postulates. The results showed that KpC4 and the clinical strain K. pneumoniae 138 （Kp138） were all highly pathogenic to maize and mice and the strain re-isolated from diseased mice also caused typical top rot symptoms on maize by artificial inoculation. It is highlighting that a seemingly dedicated human/animal pathogen could cause plant disease. This is the first report of K. pneumoniae, an opportunistic pathogen of human/animal, could infect maize and mice. The findings serve as an alert to plant, medical and veterinarian scientists regarding a potentially dangerous bacterial pathogen infecting both plants and animals/humans. The maize plants in the field could serve as a reservoir for K. pneumoniae which might infect animals and probably humans when conditions are favorable. The new findings not only are significant in the developing control strategy for the new disease in Yunnan, but also serve as a starting point for further studies on the mechanism of pathogenesis and epidemiology of K. pneumoniae.展开更多
文摘我国高粱上发生的新病害顶腐病是由亚粘团串珠镰孢(Fusarinm moniliforme var. subglutinans Wr.＆Reink.)侵染引起的。病菌生长的适宜温度为25～30℃,适宜pH值为6～8。小型分生孢子萌发的适宜温度为25～28℃,适宜pH值为6～7。病菌在Bilai培养基上,pH4～11间均易产生大型分生孢子。黑光灯和日光灯照射对大型分生孢子形成有促进作用,且黑光灯优于日光灯;在光照处理下最适宜产生大型孢子的培养基为小麦粒和高粱粒。病菌在PSA培养基上培养10～12天后可产生厚垣孢子。病菌能利用多种碳源,以半乳糖、甘露糖为佳,乳糖、木糖、葡萄糖次之。对氮源利用远不及碳源。人工接种条件下,病菌能侵染高粱、苏丹草、哥伦布草、玉米、谷子、珍珠粟、薏苡、水稻、燕麦、小麦、狗尾草等禾本科植物。
基金funded by the Maize Production System of Yunnan Province,China(2015KJTX002)
文摘Recently, a new bacterial top rot disease of maize has frequently appeared in many areas of Yunnan Province, China. The pathogen of the disease was identified as Klebsiella pneumoniae （KpC4）, which is well known to cause pulmonary and urinary diseases in humans and animals and occasionally exists as a harmless endophyte in plants. To evaluate the viru- lence of the maize pathogen to maize and mice, we inoculated maize and mice with routine inoculation and intraperitoneal injection respectively according to Koch＇s postulates. The results showed that KpC4 and the clinical strain K. pneumoniae 138 （Kp138） were all highly pathogenic to maize and mice and the strain re-isolated from diseased mice also caused typical top rot symptoms on maize by artificial inoculation. It is highlighting that a seemingly dedicated human/animal pathogen could cause plant disease. This is the first report of K. pneumoniae, an opportunistic pathogen of human/animal, could infect maize and mice. The findings serve as an alert to plant, medical and veterinarian scientists regarding a potentially dangerous bacterial pathogen infecting both plants and animals/humans. The maize plants in the field could serve as a reservoir for K. pneumoniae which might infect animals and probably humans when conditions are favorable. The new findings not only are significant in the developing control strategy for the new disease in Yunnan, but also serve as a starting point for further studies on the mechanism of pathogenesis and epidemiology of K. pneumoniae.
