AIM To evaluate effect of treatment failure on cag A and vac A genotypes in Helicobacter pylori(H. pylori) isolates from Colombia.METHODS One hundred and seventy-six participants infected with H. pylori from Colombia ...AIM To evaluate effect of treatment failure on cag A and vac A genotypes in Helicobacter pylori(H. pylori) isolates from Colombia.METHODS One hundred and seventy-six participants infected with H. pylori from Colombia were treated during 14 d with the triple-standard therapy. Six weeks later, eradication was evaluated by 13C-Urea breath test. Patients with treatment failure were subjected to endoscopy control; biopsies obtained were used for histopathology and culture. DNA from H. pylori isolates was amplified using primers specific for cag A and vac A genes. The phylogenetic relationships among isolates obtained before and after treatment were established by conglomerate analysis based on random amplified polymorphic DNA(RAPD) fingerprinting.RESULTS Treatment effectiveness was at 74.6%. Of the par-ticipants with treatment failure, 25 accepted subjected to a second endoscopy. Prevalence of posttreatment infection was 64%(16/25) and 40%(10/25) by histology and culture, respectively. Upon comparing the cag A and vac A genotypes found before and after therapy, multiple cag A genotypes(cag A-positive and cag A-negative) were found before treatment; in contrast, cag A-negative genotypes decreased after treatment. vac A s1m1 genotype was highly prevalent in patients before and after therapy. The 3'cag A region was successfully amplified in 95.5%(21/22) of the isolates obtained before and in 81.8%(18/22) of the isolates obtained after treatment. In the isolates obtained from patients with treatment failure, it was found that 72.7%(16/22) presented alterations in the number of EPIYA motifs, compared to isolates found before treatment.CONCLUSION Unsuccessful treatment limits colonization by lowvirulence strains resulting in partial and selective eradication in mixed infections, and acts on the cag A-positive strains inducing genetic rearrangements in cag A variable region that produces a loss or gain of EPIYA repetitions.展开更多
This paper presents a linear matrix inequality （LMI） approach to solve the fault-tolerant control （FTC） problem of actuator faults. The range of actuator faults is considered as a parameter region and subdivided i...This paper presents a linear matrix inequality （LMI） approach to solve the fault-tolerant control （FTC） problem of actuator faults. The range of actuator faults is considered as a parameter region and subdivided into several subregions to achieve a certain desired performance specification. Based on the integral quadratic constraint （IQC） approach, a passive fault-tolerant controller for the whole fault region and multiple fault-tolerant controllers for each fault subregion are designed for guaranteeing stability and improving performance of the FTC system, respectively. According to the estimation of parameters by FDI process, the corresponding subregion controller is chosen for the stability and optimal performance of closed-loop systems when the fault occurs. The case of incorrect estimation is also considered by comparing the performance index between the switched controller and the passive fault-tolerant controller. The proposed design technique is finally evaluated in the light of a simulation example.展开更多
Regional anesthesia is an integral component of successful orthopedic surgery.Neuraxial anesthesia is commonly used for surgical anesthesia while peripheral nerve blocks are often used for postoperative analgesia.Pati...Regional anesthesia is an integral component of successful orthopedic surgery.Neuraxial anesthesia is commonly used for surgical anesthesia while peripheral nerve blocks are often used for postoperative analgesia.Patient evaluation for regional anesthesia should include neurological,pulmonary,cardiovascular,and hematological assessments.Neuraxial blocks include spinal,epidural,and combined spinal epidural.Upper extremity peripheral nerve blocks include interscalene,supraclavicular,infraclavicular,and axillary.Lower extremity peripheral nerve blocks include femoral nerve block,saphenous nerve block,sciatic nerve block,iPACK block,ankle block and lumbar plexus block.The choice of regional anesthesia is a unanimous decision made by the surgeon,the anesthesiologist,and the patient based on a risk-benefit assessment.The choice of the regional block depends on patient cooperation,patient positing,operative structures,operative manipulation,tourniquet use and the impact of postoperative motor blockade on initiation of physical therapy.Regional anesthesia is safe but has an inherent risk of failure and a relatively low incidence of complications such as local anesthetic systemic toxicity(LAST),nerve injury,falls,hematoma,infection and allergic reactions.Ultrasound should be used for regional anesthesia procedures to improve the efficacy and minimize complications.LAST treatment guidelines and rescue medications(intralipid)should be readily available during the regional anesthesia administration.展开更多
Stability assessment is one of the most important issues in mining ground control. Mine development and/or production instability can cause production delay, loss of reserves, as well as injury to miners. Within the s...Stability assessment is one of the most important issues in mining ground control. Mine development and/or production instability can cause production delay, loss of reserves, as well as injury to miners. Within the scope of this study, a series of open stope’s instability under the influence of overlaying mined-out regions were carried out with different mining scenarios at Modi Taung gold mine which is operated by National Prosperity Gold Production Group Limited (NPGPGL) in Myanmar. NPGPGL has been developing stopes up to 150 m from the surface at Shwesin vein system, and the mining activities are going to continue to deeper levels to fulfill the ore mineral supply. Creating a new stope opening under overlaying mined-out regions is not easy considering the instability of mined-out regions can affect the stope. The instability of new stope opening is not only due to its own induced stress but also the strong influence by the mined-out regions situated on upper part of the stope. Therefore, the understandings of ground behaviors and failure mechanisms of new stope opening due to the influence of overlaying mined-out regions are paramount to be studied. This paper describes in detail the strength factor and failure zones under the overlaying mined-out regions with different mine conditions by using numerical simulations, 3D finite difference software (FLAC 3D).展开更多
文摘AIM To evaluate effect of treatment failure on cag A and vac A genotypes in Helicobacter pylori(H. pylori) isolates from Colombia.METHODS One hundred and seventy-six participants infected with H. pylori from Colombia were treated during 14 d with the triple-standard therapy. Six weeks later, eradication was evaluated by 13C-Urea breath test. Patients with treatment failure were subjected to endoscopy control; biopsies obtained were used for histopathology and culture. DNA from H. pylori isolates was amplified using primers specific for cag A and vac A genes. The phylogenetic relationships among isolates obtained before and after treatment were established by conglomerate analysis based on random amplified polymorphic DNA(RAPD) fingerprinting.RESULTS Treatment effectiveness was at 74.6%. Of the par-ticipants with treatment failure, 25 accepted subjected to a second endoscopy. Prevalence of posttreatment infection was 64%(16/25) and 40%(10/25) by histology and culture, respectively. Upon comparing the cag A and vac A genotypes found before and after therapy, multiple cag A genotypes(cag A-positive and cag A-negative) were found before treatment; in contrast, cag A-negative genotypes decreased after treatment. vac A s1m1 genotype was highly prevalent in patients before and after therapy. The 3'cag A region was successfully amplified in 95.5%(21/22) of the isolates obtained before and in 81.8%(18/22) of the isolates obtained after treatment. In the isolates obtained from patients with treatment failure, it was found that 72.7%(16/22) presented alterations in the number of EPIYA motifs, compared to isolates found before treatment.CONCLUSION Unsuccessful treatment limits colonization by lowvirulence strains resulting in partial and selective eradication in mixed infections, and acts on the cag A-positive strains inducing genetic rearrangements in cag A variable region that produces a loss or gain of EPIYA repetitions.
基金partly supported by the Program for New Century Excellent Talents in University (No.NCET-04-0283)the Funds for Creative Research Groups of China (No.60521003)+4 种基金Program for Changjiang Scholars and Innovative Research Team in University (No.IRT0421)the State Key Program of National Natural Science of China (No.60534010)the Funds of National Science of China (No.60674021)the Funds of PhD program of MOE,China (No.20060145019)the 111 Project (No.B08015)
文摘This paper presents a linear matrix inequality （LMI） approach to solve the fault-tolerant control （FTC） problem of actuator faults. The range of actuator faults is considered as a parameter region and subdivided into several subregions to achieve a certain desired performance specification. Based on the integral quadratic constraint （IQC） approach, a passive fault-tolerant controller for the whole fault region and multiple fault-tolerant controllers for each fault subregion are designed for guaranteeing stability and improving performance of the FTC system, respectively. According to the estimation of parameters by FDI process, the corresponding subregion controller is chosen for the stability and optimal performance of closed-loop systems when the fault occurs. The case of incorrect estimation is also considered by comparing the performance index between the switched controller and the passive fault-tolerant controller. The proposed design technique is finally evaluated in the light of a simulation example.
文摘Regional anesthesia is an integral component of successful orthopedic surgery.Neuraxial anesthesia is commonly used for surgical anesthesia while peripheral nerve blocks are often used for postoperative analgesia.Patient evaluation for regional anesthesia should include neurological,pulmonary,cardiovascular,and hematological assessments.Neuraxial blocks include spinal,epidural,and combined spinal epidural.Upper extremity peripheral nerve blocks include interscalene,supraclavicular,infraclavicular,and axillary.Lower extremity peripheral nerve blocks include femoral nerve block,saphenous nerve block,sciatic nerve block,iPACK block,ankle block and lumbar plexus block.The choice of regional anesthesia is a unanimous decision made by the surgeon,the anesthesiologist,and the patient based on a risk-benefit assessment.The choice of the regional block depends on patient cooperation,patient positing,operative structures,operative manipulation,tourniquet use and the impact of postoperative motor blockade on initiation of physical therapy.Regional anesthesia is safe but has an inherent risk of failure and a relatively low incidence of complications such as local anesthetic systemic toxicity(LAST),nerve injury,falls,hematoma,infection and allergic reactions.Ultrasound should be used for regional anesthesia procedures to improve the efficacy and minimize complications.LAST treatment guidelines and rescue medications(intralipid)should be readily available during the regional anesthesia administration.
文摘Stability assessment is one of the most important issues in mining ground control. Mine development and/or production instability can cause production delay, loss of reserves, as well as injury to miners. Within the scope of this study, a series of open stope’s instability under the influence of overlaying mined-out regions were carried out with different mining scenarios at Modi Taung gold mine which is operated by National Prosperity Gold Production Group Limited (NPGPGL) in Myanmar. NPGPGL has been developing stopes up to 150 m from the surface at Shwesin vein system, and the mining activities are going to continue to deeper levels to fulfill the ore mineral supply. Creating a new stope opening under overlaying mined-out regions is not easy considering the instability of mined-out regions can affect the stope. The instability of new stope opening is not only due to its own induced stress but also the strong influence by the mined-out regions situated on upper part of the stope. Therefore, the understandings of ground behaviors and failure mechanisms of new stope opening due to the influence of overlaying mined-out regions are paramount to be studied. This paper describes in detail the strength factor and failure zones under the overlaying mined-out regions with different mine conditions by using numerical simulations, 3D finite difference software (FLAC 3D).
