Hypersensitive Inhibition of the Proliferation of Cells with Mutated DNA Repair-Related Genes by the Catalytic Topoisomerase II Inhibitor 20-O-IngenolEZ

We previously reported that many ingenol compounds derived from Euphoria kansui exhibit topoisomerase inhibitory activity. 20-O-ingenolEZ in these compounds exerted inhibitory effects on both topoisomerase II (topo II) activity and cell proliferative activity. Topoisomerase II inhibitors can be divided into the poison and catalytic inhibitor types and 20-O-ingenolEZ is a catalytic inhibitor and inhibits topo IIα through inhibition of ATPase activity, but induces topo II-mediated DNA damage and apoptosis in BLM-/- DT40 cells through the induction of the DNA damage checkpoint, similar to the poison type inhibitor adriamycin. The ATPase inhibitor of topo II ICRF-193 also showed poison-like characteristics in the same cell line. However, the inhibitory effects of ICRF-193 on the proliferation of BLM-/- DT40 cells differed from those of 20-O-ingenolEZ, as did the specificity of its inhibition of the proliferation of other cell lines. 20-O-ingenolEZ showed hypersensitive inhibition of the proliferation of MCF-7 cells and BLM-/- DT40 cells with mutated DNA repair-related genes.

摆线轮的三维造型及导入ANSYS中的方法

摆线针轮减速器目前已得到广泛的应用。为了提高摆线针轮减速器的传动精度并对其做进一步的动态分析 ,要研究摆线齿轮的刚度问题。摆线齿轮齿廓是一种超越函数 ,在ANSYS中难于实现模型的建立。用Pro/E建立三维造型模型较方便 ,但在Pro/E与ANSYS之间将模型以IGES的形式进行模型转换做有限元分析时 ,由于诸多因素的影响会产生一些问题。研究了对模型进行拓扑结构修改的方法 ,然后进行网格划分作刚度分析。