Study on Callus Induction and Tissue Culture Regeneration System of Malus toringoides

[Objectives]To study the culture of callus produced by the leaves of the ethnic medicine‘E Se’,the induction of adventitious buds and the regeneration system of tissue culture seedlings.[Methods]Multi-factor orthogonal experiment was used to design and analyze.[Results]The best medium for callus induction was MS+2.0 mg/L 6-BA+0.1 mg/L 2,4-D.By comparing the effects of cytokinin 6-BA,KT and TDZ on induction of adventitious bud differentiation,it was found that the optimum medium for adventitious bud regeneration was MS+0.5 mg/L 6-BA+0.5 mg/L KT+0.15 mg/L IBA.Finally,adding 0.2 mg/L IBA(0.1 or 0.2 mg/L)to MS medium could effectively make regenerated seedlings produce roots,and the efficiency was more than 70%.[Conclusions]It laid a foundation for the large-scale cultivation of the seedlings of the ethnic medicine‘E Se’and the promotion of their artificial cultivation.

青海班玛栽培藏茶有效成分及微量元素含量分析研究

为研究青海班玛人工栽培藏茶的品质,采用药典规定方法,测定了花叶海棠和变叶海棠中茶多酚、茶碱、总黄酮、粗纤维及6种微量元素。结果表明茶多酚含量花叶海棠〉变叶海棠,茶碱的含量花叶海棠〈变叶海棠,总黄酮含量花叶海棠〈变叶海棠,粗纤维含量花叶海棠〉变叶海棠。微量元素中K、Na、Cu含量花叶海棠〉变叶海棠,Fe和Mg含量花叶海棠〈变叶海棠。