The total flavonoids of Houttuynia cordata are determined by UV-Vis spectrophotometry. The ex-traction condition of flavonoids from Houttuynia cordata includes: the extraction temperature is 70℃, solid-liquid ratio i...The total flavonoids of Houttuynia cordata are determined by UV-Vis spectrophotometry. The ex-traction condition of flavonoids from Houttuynia cordata includes: the extraction temperature is 70℃, solid-liquid ratio is 1:20, solvent is 80% ethanol, extraction time is 65 min. We studied the antioxidation of flavonoids in Houttuynia cordata, and results showed that flavonoids had strong reducing capacity, hydroxyl free radical scavenging activity and the superoxide anion radical scavenging ability, thus can provide a basis for the development of natural antioxidants.展开更多
[Objectives] To evaluate the phenolic components of Houttuynia cordata Thunb. and their antioxidant activities from different sources. [Methods] Samples of 35 different sources of H. cordata were collected from Guizho...[Objectives] To evaluate the phenolic components of Houttuynia cordata Thunb. and their antioxidant activities from different sources. [Methods] Samples of 35 different sources of H. cordata were collected from Guizhou,and polyphenols,total flavonoids,total chlorogenic acid and 7 kinds of flavonoids( xanthol-3-O-glucoside,hyperoside,quercitrin,rutin,quercetin,kaempferol and isorhamnetin),and DPPH free radical scavenging rate of the extracts were determined. Relationship between the contents of phenolics and the DPPH free radical scavenging rate of the extracts were analyzed by SPSS software. [Results] There were some significant differences in polyphenols,total flavonoids,total chlorogenic acid and the seven flavonoids between samples of different habitats,and the contents of polyphenols,total flavonoids and total chlorogenic acid increased linearly with altitude. There was a significant positive correlation between polyphenols,total flavonoids and total chlorogenic acid content and DPPH free radical scavenging rate of extracts. The contents of kaempferol-3-O-glucoside,hyperoside,quercitrin,rutin,quercetin kaempferol and isorhamnetin were positively correlated with the DPPH free radical scavenging rates of the extracts,except for the significant correlation with quercitrin. [Conclusions]The experimental results suggest that the antioxidant capacity of H. cordata is derived from the total contribution of the phenolic components,indicating that these components have overall equivalence of antioxidant capacities. This provides a new research idea for solving the key problems of quality control of traditional Chinese medicine.展开更多
以鱼腥草粉末为原材料,通过水煮法、醇提法、醇提加超声波辅助提取法得到提取液,再经离心、浓缩、抽滤后得母液浓缩液,加焦糖经喷雾干燥得鱼腥草速溶茶。以鱼腥草饮料的感官评价确定制备提取液的材料,以母液质量分数、进风温度、进料量...以鱼腥草粉末为原材料,通过水煮法、醇提法、醇提加超声波辅助提取法得到提取液,再经离心、浓缩、抽滤后得母液浓缩液,加焦糖经喷雾干燥得鱼腥草速溶茶。以鱼腥草饮料的感官评价确定制备提取液的材料,以母液质量分数、进风温度、进料量为单因素考查喷雾干燥工艺条件,以鱼腥草速溶茶成品的得率和成品中总黄酮含量为指标确定最佳工艺条件。结果表明,采用水煮法提取鱼腥草的地上部分得率较高,为10.20%,总黄酮含量为6.23%;喷雾干燥的最佳参数为母液质量分数13%,进风温度140~150℃,进料量30 m L/min。醇提加超声波辅助提取法得到的成品总黄酮含量较高,为10.12%,但此方法得到的成品得率较低,为4.5%,醇提加超声波辅助提取液的喷雾干燥最佳参数为料液质量分数1.5%,进风温度140~150℃,进料量5 m L/min。经过感官评价和黄酮含量测定证明,此鱼腥草速溶茶制备工艺可行。展开更多
文摘The total flavonoids of Houttuynia cordata are determined by UV-Vis spectrophotometry. The ex-traction condition of flavonoids from Houttuynia cordata includes: the extraction temperature is 70℃, solid-liquid ratio is 1:20, solvent is 80% ethanol, extraction time is 65 min. We studied the antioxidation of flavonoids in Houttuynia cordata, and results showed that flavonoids had strong reducing capacity, hydroxyl free radical scavenging activity and the superoxide anion radical scavenging ability, thus can provide a basis for the development of natural antioxidants.
基金Supported by National Natural Science Foundation of China(41761010,81260641)Science and Technology Foundation of Guizhou Province of China([2017]1121)Doctoral Scientific Research Foundation of Guizhou Normal University(2014)
文摘[Objectives] To evaluate the phenolic components of Houttuynia cordata Thunb. and their antioxidant activities from different sources. [Methods] Samples of 35 different sources of H. cordata were collected from Guizhou,and polyphenols,total flavonoids,total chlorogenic acid and 7 kinds of flavonoids( xanthol-3-O-glucoside,hyperoside,quercitrin,rutin,quercetin,kaempferol and isorhamnetin),and DPPH free radical scavenging rate of the extracts were determined. Relationship between the contents of phenolics and the DPPH free radical scavenging rate of the extracts were analyzed by SPSS software. [Results] There were some significant differences in polyphenols,total flavonoids,total chlorogenic acid and the seven flavonoids between samples of different habitats,and the contents of polyphenols,total flavonoids and total chlorogenic acid increased linearly with altitude. There was a significant positive correlation between polyphenols,total flavonoids and total chlorogenic acid content and DPPH free radical scavenging rate of extracts. The contents of kaempferol-3-O-glucoside,hyperoside,quercitrin,rutin,quercetin kaempferol and isorhamnetin were positively correlated with the DPPH free radical scavenging rates of the extracts,except for the significant correlation with quercitrin. [Conclusions]The experimental results suggest that the antioxidant capacity of H. cordata is derived from the total contribution of the phenolic components,indicating that these components have overall equivalence of antioxidant capacities. This provides a new research idea for solving the key problems of quality control of traditional Chinese medicine.
文摘以鱼腥草粉末为原材料,通过水煮法、醇提法、醇提加超声波辅助提取法得到提取液,再经离心、浓缩、抽滤后得母液浓缩液,加焦糖经喷雾干燥得鱼腥草速溶茶。以鱼腥草饮料的感官评价确定制备提取液的材料,以母液质量分数、进风温度、进料量为单因素考查喷雾干燥工艺条件,以鱼腥草速溶茶成品的得率和成品中总黄酮含量为指标确定最佳工艺条件。结果表明,采用水煮法提取鱼腥草的地上部分得率较高,为10.20%,总黄酮含量为6.23%;喷雾干燥的最佳参数为母液质量分数13%,进风温度140~150℃,进料量30 m L/min。醇提加超声波辅助提取法得到的成品总黄酮含量较高,为10.12%,但此方法得到的成品得率较低,为4.5%,醇提加超声波辅助提取液的喷雾干燥最佳参数为料液质量分数1.5%,进风温度140~150℃,进料量5 m L/min。经过感官评价和黄酮含量测定证明,此鱼腥草速溶茶制备工艺可行。
