Notoginseng Radix et Rhizoma(Sanqi in Chinese)is a precious traditional Chinese herbal medicine.It has the effect of dispersing blood stasis and stopping bleeding,reducing swelling and fixing pain.However,it tends to ...Notoginseng Radix et Rhizoma(Sanqi in Chinese)is a precious traditional Chinese herbal medicine.It has the effect of dispersing blood stasis and stopping bleeding,reducing swelling and fixing pain.However,it tends to contaminate with harmful fungi during storage,which may make it much less effective.In order to understand the fungal contamination of Notoginseng Radix et Rhizoma and master its composition of the exogenous fungi.The surface fungi of Notoginseng Radix et Rhizoma samples collected from six Chinese provinces and districts were investigated by using dilution plate method.Detection of aflatoxins by UPLC-MS/MS.The results showed that Penicillium citrinum was dominantly isolated from Notoginseng Radix et Rhizoma samples from No.1 to No.4.Aspergillus flavus,which produces aflatoxin,was dominantly isolated from Notoginseng Radix et Rhizoma samples from No.5 and No.6.In addition,kinds of mycotoxin were assayed which were produced by three of those identified A.flavus.All three fungi strains produced aflatoxin B1(AFB1)and one strain HBSQ1-5 additionally produced other three kinds of mycotoxin,AFB2,AFG1 and AFG2.It is the results implied that it will be very important to take serious cautions when using Notoginseng Radix et Rhizoma.As well as,understanding the composition of the exogenous fungi of Notoginseng Radix et Rhizoma and the strains of toxin-producing fungi,which can play an important role in guiding the storage of Notoginseng Radix et Rhizoma.展开更多
Traditional extraction methods of total saponins of Panax notoginseng include cold soaking method,water decoction method,alcohol reflux method,percolation method,macroporous resin adsorption method,and accelerated sol...Traditional extraction methods of total saponins of Panax notoginseng include cold soaking method,water decoction method,alcohol reflux method,percolation method,macroporous resin adsorption method,and accelerated solvent extraction( ASE) method. Modern extraction methods include ultrasonic extraction,microwave assisted extraction,supercritical CO_2 extraction,microbial fermentation assisted extraction,neural network model optimized extraction method,and multi-stage countercurrent extraction method. This paper discussed principles of these methods and compared their advantages and disadvantages.展开更多
目的分析三七总皂苷相关研究文献,掌握其发展历程、研究热点和发展趋势。方法检索中国知网、维普网、万方数据知识服务平台、中国生物医学文献和Web of Science核心合集数据库从建库至2024年4月收录的所有三七总皂苷相关中英文文献,通过...目的分析三七总皂苷相关研究文献,掌握其发展历程、研究热点和发展趋势。方法检索中国知网、维普网、万方数据知识服务平台、中国生物医学文献和Web of Science核心合集数据库从建库至2024年4月收录的所有三七总皂苷相关中英文文献,通过VOSviewer 1.6.20、CiteSpace 6.3.R1、Pajek 645.18和NoteExpress 3.8对发文量、期刊来源、作者、研究机构、关键词进行分析。结果最终纳入2010篇中文文献,其中1703篇为期刊论文,307篇为学位论文,280篇英文文献,年度发文量总体呈波动上升趋势。中文文献主要发表于《中国中药杂志》《中国实验方剂学杂志》《中草药》等,英文文献主要发表于《Acta Pharmacologica Sinica》《Journal of Ethnopharmacology》等。中文文献作者共有4410名,英文文献有1476名,中英文发文量最多的分别是邓常清和ZHANG W。北京中医药大学中英文发文量均居于榜首,与多家机构合作,形成跨区域、有重要影响力的科研学术团体。中英文文献关键词分析结果显示,细胞凋亡、氧化应激、脑出血和再灌注损伤是三七总皂苷的热门方向。结论三七总皂苷的研究内容广泛,属于学科热门关注点,很多学者仍在深度挖掘其治疗疾病的价值和作用机制,分子机制上主要聚焦于细胞凋亡、氧化应激的作用途径,疾病治疗中脑出血、再灌注损伤是当前热点研究内容。展开更多
In the present study, the potential inhibition behaviors of notoginseng total saponins(NS), safflower total flavonoids(SF), and their combination(CNS) towards three major isoforms of UDP-glucuronosyltransferases(UGTs)...In the present study, the potential inhibition behaviors of notoginseng total saponins(NS), safflower total flavonoids(SF), and their combination(CNS) towards three major isoforms of UDP-glucuronosyltransferases(UGTs) in human liver microsomes(HLMs) were investigated to study the mechanism of the synergistic effect of CNS.Etoposide, trifluoperazine and azidothymidine were selected as the probe drugs to elucidate the activities of UGT1A1, 1A4 and 2B7 by UPLC-MS/MS method, respectively.The results showed that CNS, NS and SF significantly inhibited the activities of UGT1A1, 1A4 and 2B7(P<0.05) with the IC_(50) values less than 30 mg/mL.Furthermore, the inhibitory effects of CNS towards UGT1A1, 1A4 and 2B7 were stronger than those of NS and SF(P<0.05).In conclusion, the combination of NS and SF could increase their inhibitory effects on UGT1A1, 1A4 and 2B7 activities in HLMs and might be conducive to reduce the phase II metabolism of the effective constituents in CNS.The potential herb-drug interactions of CNS based on UGT enzymes provided a useful experimental basis for its further research and development.展开更多
基金the CAMS Innovation Fund for Medical Sciences(CIFMS)(2021-I2M-1-032 and 2017-I2M-1-013)the National Key Research and Development Project(2022YFC3501504).
文摘Notoginseng Radix et Rhizoma(Sanqi in Chinese)is a precious traditional Chinese herbal medicine.It has the effect of dispersing blood stasis and stopping bleeding,reducing swelling and fixing pain.However,it tends to contaminate with harmful fungi during storage,which may make it much less effective.In order to understand the fungal contamination of Notoginseng Radix et Rhizoma and master its composition of the exogenous fungi.The surface fungi of Notoginseng Radix et Rhizoma samples collected from six Chinese provinces and districts were investigated by using dilution plate method.Detection of aflatoxins by UPLC-MS/MS.The results showed that Penicillium citrinum was dominantly isolated from Notoginseng Radix et Rhizoma samples from No.1 to No.4.Aspergillus flavus,which produces aflatoxin,was dominantly isolated from Notoginseng Radix et Rhizoma samples from No.5 and No.6.In addition,kinds of mycotoxin were assayed which were produced by three of those identified A.flavus.All three fungi strains produced aflatoxin B1(AFB1)and one strain HBSQ1-5 additionally produced other three kinds of mycotoxin,AFB2,AFG1 and AFG2.It is the results implied that it will be very important to take serious cautions when using Notoginseng Radix et Rhizoma.As well as,understanding the composition of the exogenous fungi of Notoginseng Radix et Rhizoma and the strains of toxin-producing fungi,which can play an important role in guiding the storage of Notoginseng Radix et Rhizoma.
基金Supported by the 12 th Five-Year TCM Key Discipline Chinese Medicine Chemistry Construction Program of State Administration of Traditional Chinese Medicine(Guo Zhong Yi Yao Ren Jiao Fa[2012]32)Key Discipline Chinese Medicine Chemistry Construction Program of Guangxi(Gui Jiao Ke Yan[2013]16)+2 种基金Natural Science Foundation Project of Guangxi(2013GXNSFAA019240)Program of Key Laboratory of Guangxi Universities on National Medicine in Youjiang River Basin(Gui Jiao Ke Yan[2014]14)Innovation and Entrepreneurship Ethnical Medicine Teaching Team Program of Guangxi Zhuang Autonomous Region(Gui Jiao Gao Jiao[2015]93&Gui Jiao Gao Jiao[2016]6)
文摘Traditional extraction methods of total saponins of Panax notoginseng include cold soaking method,water decoction method,alcohol reflux method,percolation method,macroporous resin adsorption method,and accelerated solvent extraction( ASE) method. Modern extraction methods include ultrasonic extraction,microwave assisted extraction,supercritical CO_2 extraction,microbial fermentation assisted extraction,neural network model optimized extraction method,and multi-stage countercurrent extraction method. This paper discussed principles of these methods and compared their advantages and disadvantages.
基金National Natural Science Foundation of China(Grant No.81573684)National Key Technology R&D Program "New Drug Innovation" of China(Grant No.2018ZX09711001-008-003)Beijing Municipal Science and Technology Project(Grant No.Z181100002218028)
文摘In the present study, the potential inhibition behaviors of notoginseng total saponins(NS), safflower total flavonoids(SF), and their combination(CNS) towards three major isoforms of UDP-glucuronosyltransferases(UGTs) in human liver microsomes(HLMs) were investigated to study the mechanism of the synergistic effect of CNS.Etoposide, trifluoperazine and azidothymidine were selected as the probe drugs to elucidate the activities of UGT1A1, 1A4 and 2B7 by UPLC-MS/MS method, respectively.The results showed that CNS, NS and SF significantly inhibited the activities of UGT1A1, 1A4 and 2B7(P<0.05) with the IC_(50) values less than 30 mg/mL.Furthermore, the inhibitory effects of CNS towards UGT1A1, 1A4 and 2B7 were stronger than those of NS and SF(P<0.05).In conclusion, the combination of NS and SF could increase their inhibitory effects on UGT1A1, 1A4 and 2B7 activities in HLMs and might be conducive to reduce the phase II metabolism of the effective constituents in CNS.The potential herb-drug interactions of CNS based on UGT enzymes provided a useful experimental basis for its further research and development.