[Objectives]To optimize the extraction process of total flavonoids from Eucommiae Cortex,establish the extraction and content determination methods of its medicinal materials,and study its in vitro antioxidant activit...[Objectives]To optimize the extraction process of total flavonoids from Eucommiae Cortex,establish the extraction and content determination methods of its medicinal materials,and study its in vitro antioxidant activity.[Methods]The total flavonoids from Eucommiae Cortex were extracted by reflux extraction method,and the effects of extraction method,extraction solvent concentration,extraction volume,and extraction time on the total flavonoids content of the medicinal materials were explored through the single factor experiment.Orthogonal experiment was carried out to optimize the extraction process conditions,and the optimal extraction process for total flavonoids from Eucommiae Cortex was screened out.The total antioxidant capacity index was used to determine the free radical scavenging ability of the total flavonoids from Eucommiae Cortex.[Results]The optimal extraction process of total flavonoids from Eucommiae Cortex was 50%ethanol,1∶40 solid-to-liquid-ratio,and 1.5 h reflux extraction time.Through the antioxidant experiment in vitro,it is found that the total flavonoids from Eucommiae Cortex have a higher scavenging ability for the total antioxidant capacity,and there is a certain positive correlation with the mass concentration of total flavonoids.[Conclusions]This method can effectively determine the total flavonoids content of Eucommiae Cortex medicinal material,and provide a certain scientific basis for the quality standard research of the medicinal material.The total flavonoids from Eucommiae Cortex have good in vitro antioxidant activity.And the method for extracting total flavonoids from Eucommiae Cortex medicinal material in this experiment has good repeatability,high stability and feasibility.展开更多
对杜仲(Eucomm ia ulm oidesO liv.)雄株不同花期雄花中次生代谢产物含量进行分析测定,并对杜仲雄花次生代谢的生理基础及不同花期次生代谢产物含量差异进行了探讨。研究结果表明,雄花在不同花期次生代谢产物含量均有差异。总黄酮含量...对杜仲(Eucomm ia ulm oidesO liv.)雄株不同花期雄花中次生代谢产物含量进行分析测定,并对杜仲雄花次生代谢的生理基础及不同花期次生代谢产物含量差异进行了探讨。研究结果表明,雄花在不同花期次生代谢产物含量均有差异。总黄酮含量在花蕾期最高(4.010%),始花期最低(2.422%),从盛花期到末花期逐渐上升;桃叶珊瑚苷和绿原酸含量均在花蕾期最高(分别为2.351%和1.075%),盛花期最低(分别为1.463%和0.503%),至末花期含量上升;京尼平苷酸含量在始花期最低(0.217%),从盛花期开始逐渐升高,至末花期高达1.403%;次生代谢产物总量也以花蕾期为最高(7.420%)。杜仲雄花的花蕾期和盛花期是兼顾质量和产量的最佳采摘期。展开更多
基金Supported by Special Project for Central Leading Local Science and Technology Development in Sichuan Province:Southwestern Characteristic Chinese Medicine Resources Genetics Innovation Platform(2020ZYD058)Young Teachers Enhancement Project of Guangxi Department of Education in 2018(2018KY0300)2019-2021 Guangxi First-class Discipline Construction Open Project Fund for Young Scholars of Guangxi University of Chinese Medicine(2019XK089).
文摘[Objectives]To optimize the extraction process of total flavonoids from Eucommiae Cortex,establish the extraction and content determination methods of its medicinal materials,and study its in vitro antioxidant activity.[Methods]The total flavonoids from Eucommiae Cortex were extracted by reflux extraction method,and the effects of extraction method,extraction solvent concentration,extraction volume,and extraction time on the total flavonoids content of the medicinal materials were explored through the single factor experiment.Orthogonal experiment was carried out to optimize the extraction process conditions,and the optimal extraction process for total flavonoids from Eucommiae Cortex was screened out.The total antioxidant capacity index was used to determine the free radical scavenging ability of the total flavonoids from Eucommiae Cortex.[Results]The optimal extraction process of total flavonoids from Eucommiae Cortex was 50%ethanol,1∶40 solid-to-liquid-ratio,and 1.5 h reflux extraction time.Through the antioxidant experiment in vitro,it is found that the total flavonoids from Eucommiae Cortex have a higher scavenging ability for the total antioxidant capacity,and there is a certain positive correlation with the mass concentration of total flavonoids.[Conclusions]This method can effectively determine the total flavonoids content of Eucommiae Cortex medicinal material,and provide a certain scientific basis for the quality standard research of the medicinal material.The total flavonoids from Eucommiae Cortex have good in vitro antioxidant activity.And the method for extracting total flavonoids from Eucommiae Cortex medicinal material in this experiment has good repeatability,high stability and feasibility.
文摘对杜仲(Eucomm ia ulm oidesO liv.)雄株不同花期雄花中次生代谢产物含量进行分析测定,并对杜仲雄花次生代谢的生理基础及不同花期次生代谢产物含量差异进行了探讨。研究结果表明,雄花在不同花期次生代谢产物含量均有差异。总黄酮含量在花蕾期最高(4.010%),始花期最低(2.422%),从盛花期到末花期逐渐上升;桃叶珊瑚苷和绿原酸含量均在花蕾期最高(分别为2.351%和1.075%),盛花期最低(分别为1.463%和0.503%),至末花期含量上升;京尼平苷酸含量在始花期最低(0.217%),从盛花期开始逐渐升高,至末花期高达1.403%;次生代谢产物总量也以花蕾期为最高(7.420%)。杜仲雄花的花蕾期和盛花期是兼顾质量和产量的最佳采摘期。
