Objective:The objective was to study the effect of total flavonoids of Herba Taxilli(TFHT)on osteoporotic(OP)rats induced by retinoic acid(RA)and explore its mechanism.Methods:Three-month-old female Sprague Dawley rat...Objective:The objective was to study the effect of total flavonoids of Herba Taxilli(TFHT)on osteoporotic(OP)rats induced by retinoic acid(RA)and explore its mechanism.Methods:Three-month-old female Sprague Dawley rats were randomly divided into six groups,each with ten rats,i.e.normal control group,model group,and positive control group and three TFHT groups(at high-,middle-,and low-doses).Except the normal control group,rats of all the other groups were administered RA by gavage(70 mg/kg/day)every morning for 14 days to establish an OP model;at the same time,all the rats were given Corresponding therapeutic drug by gavage for 28 days:rats in the three TFHT groups were given TFHT(400,200,and 100 mg/kg/day,respectively)and rats in the positive control group were given Long mu Zhuang gu Granule 300 mg/kg/day.Rats in the model group and normal group were given an equal volume of 1%sodium carboxymethyl cellulose sodium suspension.After the experiment,blood was taken to measure the levels of serum calcium(Ca^(2+)),alkaline phosphatase(ALP),and tartrate-resistant acid phosphatase(TRAP);the spleen,uterus,and ovary were dissected to determine the organ coefficient;the left hind limb femur was dissected to determine bone mineral density(BMD),bone dry weight(BDW),bone wet weight(BWW),bone diameter(BD),bone length(BL),bone dry weight/rat weight(BDW/W),bone wet weight/rat weight(BWW/W),bone dry weight/bone wet weight(BDW/BWW),bone dry weight/bone length(BDW/L),and bone wet weight/bone diameter(BWW/D);and the right hind limb femur was dissected to observe the bone path morphological changes.Results:Compared with the model group,the serum Ca^(2+)content,uterus plus ovary coefficient,BMD,BDW,BDW/W,BWW/W,BDW/BWW,BDW/L,and BWW/D in the TFHT groups increased,whereas the spleen coefficient,ALP,and TRAP decreased in the TFHT groups,and the differences were statistically significant(P<0.05 or P<0.01).Conclusion:TFHT was found effective in treating OP in rats induced by RA;the main mechanisms were increasing the serum Ca^(2+)content and reducing the level of ALP and TRAP.展开更多
文摘Objective:The objective was to study the effect of total flavonoids of Herba Taxilli(TFHT)on osteoporotic(OP)rats induced by retinoic acid(RA)and explore its mechanism.Methods:Three-month-old female Sprague Dawley rats were randomly divided into six groups,each with ten rats,i.e.normal control group,model group,and positive control group and three TFHT groups(at high-,middle-,and low-doses).Except the normal control group,rats of all the other groups were administered RA by gavage(70 mg/kg/day)every morning for 14 days to establish an OP model;at the same time,all the rats were given Corresponding therapeutic drug by gavage for 28 days:rats in the three TFHT groups were given TFHT(400,200,and 100 mg/kg/day,respectively)and rats in the positive control group were given Long mu Zhuang gu Granule 300 mg/kg/day.Rats in the model group and normal group were given an equal volume of 1%sodium carboxymethyl cellulose sodium suspension.After the experiment,blood was taken to measure the levels of serum calcium(Ca^(2+)),alkaline phosphatase(ALP),and tartrate-resistant acid phosphatase(TRAP);the spleen,uterus,and ovary were dissected to determine the organ coefficient;the left hind limb femur was dissected to determine bone mineral density(BMD),bone dry weight(BDW),bone wet weight(BWW),bone diameter(BD),bone length(BL),bone dry weight/rat weight(BDW/W),bone wet weight/rat weight(BWW/W),bone dry weight/bone wet weight(BDW/BWW),bone dry weight/bone length(BDW/L),and bone wet weight/bone diameter(BWW/D);and the right hind limb femur was dissected to observe the bone path morphological changes.Results:Compared with the model group,the serum Ca^(2+)content,uterus plus ovary coefficient,BMD,BDW,BDW/W,BWW/W,BDW/BWW,BDW/L,and BWW/D in the TFHT groups increased,whereas the spleen coefficient,ALP,and TRAP decreased in the TFHT groups,and the differences were statistically significant(P<0.05 or P<0.01).Conclusion:TFHT was found effective in treating OP in rats induced by RA;the main mechanisms were increasing the serum Ca^(2+)content and reducing the level of ALP and TRAP.
