Objective: To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids(CONR) on myocardial apoptosis of diabetic atherosclerosis(DA) rabbits Method...Objective: To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids(CONR) on myocardial apoptosis of diabetic atherosclerosis(DA) rabbits Methods: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group(450 mg/kg), CONR medium-dose group(150 mg/kg), CONR low-dose group(50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group. DA model was established by intravenously injected alloxan combined with high-fat diet and abdominal aortic balloon injury. After mediation for 10 weeks, fasting blood glucose(FBG), glycosylated hemoglobin(GHB), glycosylated serum protein(GSP), fructoseamine(FRA), aldose reductase(AR), advanced glycation end products(AGEs) in serum were measured by enzyme linked immunosorbent assay(ELISA) method;the expression of receptor of AGEs(RAGE) in myocardial tissue were observed by immunohistochemical method;and p-Jun N-terminal kinase(p-JNK), caspase-3, B-cell lymphoma-2(bcl-2) protein expression in myocardial tissue were measured by Western blotting. The myocardial apoptosis was detected by Td T-mediated d UTPnick-end labeling(TUNEL) method, and apoptosis index(AI) was calculated. Results: Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index(AI) were significantly increased and bcl-2 protein was significantly decreased in the model group(P<0.01). Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high-and medium-dose groups(P<0.01). FBG and GHB showed a significant decline in CONR low-dose group(P<0.01). Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups(P<0.01);high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups(P<0.01). Middle-dose CONR had better effect on serum AGEs compared with the low-dose group(P<0.01);middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group(P<0.01). Conclusion: CONR may effectively inhibit myocardial apoptosis on DA rabbits by intervening AGEs-RAGE and JNK, caspase-3, and bcl-2 protein expressions.展开更多
Drying is a basic link in seafood processing, and the effects of forced air drying and vacuum drying on the effective components of Stichopus japonicus viscera were compared with the moisture, total saponin and polysa...Drying is a basic link in seafood processing, and the effects of forced air drying and vacuum drying on the effective components of Stichopus japonicus viscera were compared with the moisture, total saponin and polysaccharide contents as the detection indexes. The contents of effective components obtained using forced air drying were slightly lower than those obtained using vacuum drying, but the forced air drying method used short drying time and low economic energy consumption. Excellent drying effects and low cost of forced air drying made it can be adapted to the requirements of large-scale production applications.展开更多
通过人参水提液(water extract of Ginseng,WEG)、人参多糖(Ginseng polysaccharides,GPS)、人参皂苷(Ginseng total saponins,GTS)对肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)与肿瘤细胞共培养体系中肺癌A549细胞增殖、迁...通过人参水提液(water extract of Ginseng,WEG)、人参多糖(Ginseng polysaccharides,GPS)、人参皂苷(Ginseng total saponins,GTS)对肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)与肿瘤细胞共培养体系中肺癌A549细胞增殖、迁移和细胞骨架的影响研究,探讨人参抗肿瘤的作用机制。建立THP-1诱导的TAMs体外模型,采用上清液共培养体系,以肺癌A549细胞为研究对象,并采用MTT法观察不同浓度的WEG、GPS、GTS作用于共培养体系中对肺癌A549细胞增殖的影响及量效关系;通过实时细胞分析技术(RTCA)检测WEG、GPS、GTS对共培养体系中肺癌A549细胞迁移能力的影响,免疫荧光高内涵细胞分析系统(HCS)检测细胞骨架蛋白F-actin的表达。结果显示:WEG能明显抑制共培养体系中A549细胞的增殖、迁移能力和减少骨架面积及微丝数量(P<0.01);GPS能明显抑制共培养体系中A549细胞迁移能力、骨架面积和微丝数量(P<0.01),但对A549细胞的增殖作用无明显影响;GTS能明显抑制共培养体系中A549细胞的增殖(P<0.01),而对A549细胞的迁移能力、骨架面积和微丝无影响。通过对WEG、GPS、GTS的研究对比,发现人参及两种主要成分对TAMs共培养体系中肺癌A549细胞都有影响,但作用存在差异,提示人参抗肿瘤作用与其多成分对肿瘤免疫微环境调控相关,且不同成分间可能存在协同关系。展开更多
基金Supported by National Natural Science Foundation of China(No.81374026)。
文摘Objective: To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids(CONR) on myocardial apoptosis of diabetic atherosclerosis(DA) rabbits Methods: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group(450 mg/kg), CONR medium-dose group(150 mg/kg), CONR low-dose group(50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group. DA model was established by intravenously injected alloxan combined with high-fat diet and abdominal aortic balloon injury. After mediation for 10 weeks, fasting blood glucose(FBG), glycosylated hemoglobin(GHB), glycosylated serum protein(GSP), fructoseamine(FRA), aldose reductase(AR), advanced glycation end products(AGEs) in serum were measured by enzyme linked immunosorbent assay(ELISA) method;the expression of receptor of AGEs(RAGE) in myocardial tissue were observed by immunohistochemical method;and p-Jun N-terminal kinase(p-JNK), caspase-3, B-cell lymphoma-2(bcl-2) protein expression in myocardial tissue were measured by Western blotting. The myocardial apoptosis was detected by Td T-mediated d UTPnick-end labeling(TUNEL) method, and apoptosis index(AI) was calculated. Results: Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index(AI) were significantly increased and bcl-2 protein was significantly decreased in the model group(P<0.01). Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high-and medium-dose groups(P<0.01). FBG and GHB showed a significant decline in CONR low-dose group(P<0.01). Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups(P<0.01);high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups(P<0.01). Middle-dose CONR had better effect on serum AGEs compared with the low-dose group(P<0.01);middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group(P<0.01). Conclusion: CONR may effectively inhibit myocardial apoptosis on DA rabbits by intervening AGEs-RAGE and JNK, caspase-3, and bcl-2 protein expressions.
文摘Drying is a basic link in seafood processing, and the effects of forced air drying and vacuum drying on the effective components of Stichopus japonicus viscera were compared with the moisture, total saponin and polysaccharide contents as the detection indexes. The contents of effective components obtained using forced air drying were slightly lower than those obtained using vacuum drying, but the forced air drying method used short drying time and low economic energy consumption. Excellent drying effects and low cost of forced air drying made it can be adapted to the requirements of large-scale production applications.
文摘通过人参水提液(water extract of Ginseng,WEG)、人参多糖(Ginseng polysaccharides,GPS)、人参皂苷(Ginseng total saponins,GTS)对肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)与肿瘤细胞共培养体系中肺癌A549细胞增殖、迁移和细胞骨架的影响研究,探讨人参抗肿瘤的作用机制。建立THP-1诱导的TAMs体外模型,采用上清液共培养体系,以肺癌A549细胞为研究对象,并采用MTT法观察不同浓度的WEG、GPS、GTS作用于共培养体系中对肺癌A549细胞增殖的影响及量效关系;通过实时细胞分析技术(RTCA)检测WEG、GPS、GTS对共培养体系中肺癌A549细胞迁移能力的影响,免疫荧光高内涵细胞分析系统(HCS)检测细胞骨架蛋白F-actin的表达。结果显示:WEG能明显抑制共培养体系中A549细胞的增殖、迁移能力和减少骨架面积及微丝数量(P<0.01);GPS能明显抑制共培养体系中A549细胞迁移能力、骨架面积和微丝数量(P<0.01),但对A549细胞的增殖作用无明显影响;GTS能明显抑制共培养体系中A549细胞的增殖(P<0.01),而对A549细胞的迁移能力、骨架面积和微丝无影响。通过对WEG、GPS、GTS的研究对比,发现人参及两种主要成分对TAMs共培养体系中肺癌A549细胞都有影响,但作用存在差异,提示人参抗肿瘤作用与其多成分对肿瘤免疫微环境调控相关,且不同成分间可能存在协同关系。
