The present work aimed to investigate the microscopic and ultramicroscopic structure of the liver to assess the level of hepatic impairment during pregnancy toxemia. Seven pregnant small-tailed Han sheep of negative u...The present work aimed to investigate the microscopic and ultramicroscopic structure of the liver to assess the level of hepatic impairment during pregnancy toxemia. Seven pregnant small-tailed Han sheep of negative urine ketone bodies were assessed in this study. Toxemia was induced by limiting food and movement late in pregnancy. Three sheep developed obvious clinical symptoms with motor weakness, depression, anorexia, locomotion disturbances, blindness and languishment. We harvested their liver tissues as pathological material, and used routine histological and electronic microscopy methods to observe the histopathological changes in small-tailed Han sheep induced by pregnancy toxemia. Autopsy of the livers of the sheep revealed deep yellow coloration, intumescence and hemorrhage on the surface. Microstructural features indicated fatty degeneration, which is a main characteristic of fatty liver. Hepatocellular ultrastructural changes were observed under an electronic microscope. The characteristic findings were nucleolus concentration, vacuolation of mitochondria and excessive glycogen granules in the cytoplasm. Via this experimental protocol, pregnancy toxemia of sheep was successfully induced, providing a pathological model for the study of this disease. After the experimental induction of pregnancy toxemia, the clinical symptoms of pathogenic sheep and pathological changes to their livers exhibited obvious characteristics of pregnancy toxemia.展开更多
Background: Advanced maternal age, over 35 years, is a well-known risk factor for poor pregnancy outcomes. It is commonly associated with various pregnancy complications, such as spontaneous miscarriage, preeclampsia/...Background: Advanced maternal age, over 35 years, is a well-known risk factor for poor pregnancy outcomes. It is commonly associated with various pregnancy complications, such as spontaneous miscarriage, preeclampsia/ toxemia, gestational diabetes mellitus, preterm labor, stillbirth, chromosomal abnormalities, and cesarean delivery. Objectives: This study assessed obstetric and neonatal complications associated with advanced maternal age. Methods: We reviewed the medical records of 199 pregnant women over 35 years old at King Abdulaziz University Hospital in Jeddah, Saudi Arabia, from January to June 2022. We gathered data on age, nationality, number of antenatal visits, results of ultrasound scans for dating and viability, nuchal translucency and anatomy surveys, medications and multivitamins taken during pregnancy, smoking status, pregnancy, and fetal complications, and mode of delivery. Results: The prevalence of obstetric complications was 71.4% (preeclampsia/toxemia, 4.5%;antepartum hemorrhage, 4%;postpartum hemorrhage, 1%;and gestational diabetes, 23.1%). The most frequent complication was preterm labor between 34 and 36 weeks (48%), and only 12.6% of all deliveries were associated with fetal and neonatal complications such as congenital anomalies and neonatal jaundice. The prevalence of anemia during pregnancy was 10.1%, 21.1%, and 28.6% in the first, second, and third trimesters, respectively, and pregnancies complicated with antepartum or postpartum hemorrhage were associated with higher rates of anemia in the second trimester. A significant relationship was found between mean maternal age (38.84 ± 2.75 years) and the development of maternal complications (p < 0.05). Newborns with neonatal complications were much more likely to be born to mothers with a history of antepartum hemorrhage and anemia in the second trimester. Conclusion: Our findings confirm that pregnancy at an advanced maternal age is associated with increased overall maternal complications. The most frequent complication was preterm labor (48%). Other complications, such as preeclampsia/toxemia, antepartum hemorrhage, postpartum hemorrhage, gestational diabetes, and anemia, were less frequent in the sample we reviewed.展开更多
本研究旨在评估过瘤胃胆碱(RPC)和过瘤胃葡萄糖(RPG)对围产期母羊能量负平衡及其产羔性能的影响。选取80头湖羊,随机分为4组,每组20头,对照组饲喂基础饲粮,RPG组、RPC组和RPC+RPG组每头每天分别添加15 g RPG、3 g RPC、15 g RPG+3 g RP...本研究旨在评估过瘤胃胆碱(RPC)和过瘤胃葡萄糖(RPG)对围产期母羊能量负平衡及其产羔性能的影响。选取80头湖羊,随机分为4组,每组20头,对照组饲喂基础饲粮,RPG组、RPC组和RPC+RPG组每头每天分别添加15 g RPG、3 g RPC、15 g RPG+3 g RPC。试验期50 d,结果表明:1)各组间妊娠毒血症的发病率无显著差异(P>0.05),但与对照组相比,试验组有提高羔羊断奶成活率的趋势(0.10>P>0.05),且RPC+RPG组羔羊初生重显著提高(P<0.05)。2)与对照组相比,添加RPG显著降低了分娩当天母羊血清中β-羟基丁酸含量(P<0.05),同时血清中总蛋白含量也显著提高(P<0.05),还显著提高了产后10 d时血清甲状腺素(T4)含量(P<0.05),对血清葡萄糖含量影响不显著(P>0.05)。3)与对照组相比,添加RPC对血清葡萄糖和β-羟基丁酸含量影响均不显著(P>0.05),相反还显著提高了分娩当天和产后20 d的血清谷丙转氨酶活性(P<0.05),添加RPC+RPG对血清生化指标无显著交互作用(P>0.05)。综上所述,围产期母羊饲粮中添加15 g/(d•头)RPG有助于缓解能量负平衡,而3 g/(d•头)RPC添加效果不显著,但添加RPC+RPG均可以提升羔羊断奶成活率,提高羔羊初生重。展开更多
目的探讨IFIT1(interferon-induced protein with tetratricopetide repeats 1)在放射损伤与感染应激中表达的特点和意义。方法采用5μg/mL的脂多糖(LPS)刺激Raw264.7、3T3和10T1/2细胞株5 h后,收集细胞,提取RNA,行RT-PCR观察IFIT1的表...目的探讨IFIT1(interferon-induced protein with tetratricopetide repeats 1)在放射损伤与感染应激中表达的特点和意义。方法采用5μg/mL的脂多糖(LPS)刺激Raw264.7、3T3和10T1/2细胞株5 h后,收集细胞,提取RNA,行RT-PCR观察IFIT1的表达情况,同时设定空白对照组。另外,将20只C57/BL6小鼠随机分为4组,采用12 Gray60Co一次性全身照射,分别于0 h、1 h、4 h和12 h脱颈处死取肝组织,用Western blot测定肝组织IFIT1表达变化。结果 Raw264.7、3T3和10T1/2细胞株经LPS刺激5 h后,均能诱导IFIT1表达,空白对照组表达为阴性。C57/BL6小鼠放射性损伤后,1 h IFIT1/Actin开始增高,12 h达最高,呈上升趋势,差异有极显著性统计学意义(P<0.01)。结论 LPS可刺激多种细胞株表达IFIT1,提示IFIT1可能参与创伤后毒血症的发生和发展;放射损伤早期能引起小鼠肝组织IFIT1的显著增高。展开更多
基金Supported by Natural Science Foundation of Ningxia(BA002-2004)
文摘The present work aimed to investigate the microscopic and ultramicroscopic structure of the liver to assess the level of hepatic impairment during pregnancy toxemia. Seven pregnant small-tailed Han sheep of negative urine ketone bodies were assessed in this study. Toxemia was induced by limiting food and movement late in pregnancy. Three sheep developed obvious clinical symptoms with motor weakness, depression, anorexia, locomotion disturbances, blindness and languishment. We harvested their liver tissues as pathological material, and used routine histological and electronic microscopy methods to observe the histopathological changes in small-tailed Han sheep induced by pregnancy toxemia. Autopsy of the livers of the sheep revealed deep yellow coloration, intumescence and hemorrhage on the surface. Microstructural features indicated fatty degeneration, which is a main characteristic of fatty liver. Hepatocellular ultrastructural changes were observed under an electronic microscope. The characteristic findings were nucleolus concentration, vacuolation of mitochondria and excessive glycogen granules in the cytoplasm. Via this experimental protocol, pregnancy toxemia of sheep was successfully induced, providing a pathological model for the study of this disease. After the experimental induction of pregnancy toxemia, the clinical symptoms of pathogenic sheep and pathological changes to their livers exhibited obvious characteristics of pregnancy toxemia.
文摘Background: Advanced maternal age, over 35 years, is a well-known risk factor for poor pregnancy outcomes. It is commonly associated with various pregnancy complications, such as spontaneous miscarriage, preeclampsia/ toxemia, gestational diabetes mellitus, preterm labor, stillbirth, chromosomal abnormalities, and cesarean delivery. Objectives: This study assessed obstetric and neonatal complications associated with advanced maternal age. Methods: We reviewed the medical records of 199 pregnant women over 35 years old at King Abdulaziz University Hospital in Jeddah, Saudi Arabia, from January to June 2022. We gathered data on age, nationality, number of antenatal visits, results of ultrasound scans for dating and viability, nuchal translucency and anatomy surveys, medications and multivitamins taken during pregnancy, smoking status, pregnancy, and fetal complications, and mode of delivery. Results: The prevalence of obstetric complications was 71.4% (preeclampsia/toxemia, 4.5%;antepartum hemorrhage, 4%;postpartum hemorrhage, 1%;and gestational diabetes, 23.1%). The most frequent complication was preterm labor between 34 and 36 weeks (48%), and only 12.6% of all deliveries were associated with fetal and neonatal complications such as congenital anomalies and neonatal jaundice. The prevalence of anemia during pregnancy was 10.1%, 21.1%, and 28.6% in the first, second, and third trimesters, respectively, and pregnancies complicated with antepartum or postpartum hemorrhage were associated with higher rates of anemia in the second trimester. A significant relationship was found between mean maternal age (38.84 ± 2.75 years) and the development of maternal complications (p < 0.05). Newborns with neonatal complications were much more likely to be born to mothers with a history of antepartum hemorrhage and anemia in the second trimester. Conclusion: Our findings confirm that pregnancy at an advanced maternal age is associated with increased overall maternal complications. The most frequent complication was preterm labor (48%). Other complications, such as preeclampsia/toxemia, antepartum hemorrhage, postpartum hemorrhage, gestational diabetes, and anemia, were less frequent in the sample we reviewed.
文摘本研究旨在评估过瘤胃胆碱(RPC)和过瘤胃葡萄糖(RPG)对围产期母羊能量负平衡及其产羔性能的影响。选取80头湖羊,随机分为4组,每组20头,对照组饲喂基础饲粮,RPG组、RPC组和RPC+RPG组每头每天分别添加15 g RPG、3 g RPC、15 g RPG+3 g RPC。试验期50 d,结果表明:1)各组间妊娠毒血症的发病率无显著差异(P>0.05),但与对照组相比,试验组有提高羔羊断奶成活率的趋势(0.10>P>0.05),且RPC+RPG组羔羊初生重显著提高(P<0.05)。2)与对照组相比,添加RPG显著降低了分娩当天母羊血清中β-羟基丁酸含量(P<0.05),同时血清中总蛋白含量也显著提高(P<0.05),还显著提高了产后10 d时血清甲状腺素(T4)含量(P<0.05),对血清葡萄糖含量影响不显著(P>0.05)。3)与对照组相比,添加RPC对血清葡萄糖和β-羟基丁酸含量影响均不显著(P>0.05),相反还显著提高了分娩当天和产后20 d的血清谷丙转氨酶活性(P<0.05),添加RPC+RPG对血清生化指标无显著交互作用(P>0.05)。综上所述,围产期母羊饲粮中添加15 g/(d•头)RPG有助于缓解能量负平衡,而3 g/(d•头)RPC添加效果不显著,但添加RPC+RPG均可以提升羔羊断奶成活率,提高羔羊初生重。
