[Objective] This paper was aimed to study the biological characteristics of Colletotrichum orbiculare（Berk.et Mont.） Arx, and accomplish the preliminary screening the test of fungicides. [Method]The pathogen was iso...[Objective] This paper was aimed to study the biological characteristics of Colletotrichum orbiculare（Berk.et Mont.） Arx, and accomplish the preliminary screening the test of fungicides. [Method]The pathogen was isolated from the infected leaves of watermelon collected from greenhouse in Hainan Province. The biological characteristics of C. orbiculare and indoor toxicity of fungicides were studied. [Results] The mycelium grew fastest on the medium with leaf extract of watermelon. The optimum temperature for mycelial growth and spore germination was 28 and 30 ℃, and the optimum pH was 7-9 and 6-8, respectively. The continuous darkness condition was most suitable for mycelial growth. The lethal temperature of spore was 55 ℃ water bath for 10 min in water bath. The suitable carbon sources were maltose, L-arabinose and glucose, and the suitable nitrogen sources were yeast extract, beef extract and peptone. In the indoor toxicity determination, 32.5% Benzoic azoxystrobin SC, 50% Dichlorotetrakis WP and 10% Difenoconazole WG showed better inhibition effects against the mycelial growth of C. orbiculare, with the EC50 values of 0.018 0, 0.060 2 and 0.471 5 μg/ml, respectively. [Conclusion]The research provided theoretical basis for the control of watermelon anthracnose in watermelon fields.展开更多
[Objective] The paper was to isolate Colletotrichum sp.from strawberry and to screen effective fungicides.[Method] The indoor toxicity of 7 fungicides to Colletotrichum sp.was measured by growth rate method.The potted...[Objective] The paper was to isolate Colletotrichum sp.from strawberry and to screen effective fungicides.[Method] The indoor toxicity of 7 fungicides to Colletotrichum sp.was measured by growth rate method.The potted control effect of 3 fungicides on Colletotrichum sp.was evaluated.[Result] Fludioxonil exhibited the highest indoor toxicity to Colletotrichum sp.,with the EC50 value of 0.117 mg/L,and the EC50 value of prochloraz and difenoconazole were 0.141 and 0.357 mg/L,respectively.The pot experiment showed that the potted control effect of 20% fludioxonil2 000 times dilution on Colletotrichum sp.was the highest of 92.86%.450 g/L prochloraz EW 4 500 times dilution and 250 g/L difenoconazole EC2 500 times dilution also received high control effects against Colletotrichum sp.,which were 91.68% and 89.29%,respectively.[Conclusion] Fludioxonil,prochloraz and difenoconazole could be used for the control of strawberry anthracnose.展开更多
基金Supported by Scientific Funds Project of Hainan Province[(QCY(2009)71]Local Standards of Hainan Province in 2011 "Technical Regulation of Greenhouse Watermelon Production"Project[QZJJ(2011)11]
文摘[Objective] This paper was aimed to study the biological characteristics of Colletotrichum orbiculare（Berk.et Mont.） Arx, and accomplish the preliminary screening the test of fungicides. [Method]The pathogen was isolated from the infected leaves of watermelon collected from greenhouse in Hainan Province. The biological characteristics of C. orbiculare and indoor toxicity of fungicides were studied. [Results] The mycelium grew fastest on the medium with leaf extract of watermelon. The optimum temperature for mycelial growth and spore germination was 28 and 30 ℃, and the optimum pH was 7-9 and 6-8, respectively. The continuous darkness condition was most suitable for mycelial growth. The lethal temperature of spore was 55 ℃ water bath for 10 min in water bath. The suitable carbon sources were maltose, L-arabinose and glucose, and the suitable nitrogen sources were yeast extract, beef extract and peptone. In the indoor toxicity determination, 32.5% Benzoic azoxystrobin SC, 50% Dichlorotetrakis WP and 10% Difenoconazole WG showed better inhibition effects against the mycelial growth of C. orbiculare, with the EC50 values of 0.018 0, 0.060 2 and 0.471 5 μg/ml, respectively. [Conclusion]The research provided theoretical basis for the control of watermelon anthracnose in watermelon fields.
基金Supported by Overseas Talents Introduction Project of Shandong Academy of Agricultural Sciences(CXGC2021B04)。
文摘[Objective] The paper was to isolate Colletotrichum sp.from strawberry and to screen effective fungicides.[Method] The indoor toxicity of 7 fungicides to Colletotrichum sp.was measured by growth rate method.The potted control effect of 3 fungicides on Colletotrichum sp.was evaluated.[Result] Fludioxonil exhibited the highest indoor toxicity to Colletotrichum sp.,with the EC50 value of 0.117 mg/L,and the EC50 value of prochloraz and difenoconazole were 0.141 and 0.357 mg/L,respectively.The pot experiment showed that the potted control effect of 20% fludioxonil2 000 times dilution on Colletotrichum sp.was the highest of 92.86%.450 g/L prochloraz EW 4 500 times dilution and 250 g/L difenoconazole EC2 500 times dilution also received high control effects against Colletotrichum sp.,which were 91.68% and 89.29%,respectively.[Conclusion] Fludioxonil,prochloraz and difenoconazole could be used for the control of strawberry anthracnose.
