Several researchers have investigated the association of numerous opportunistic pathogens with HIV, little is documented on its association with T. gondii in our environment. We investigated the prevalence of T. gondi...Several researchers have investigated the association of numerous opportunistic pathogens with HIV, little is documented on its association with T. gondii in our environment. We investigated the prevalence of T. gondii immunoglobulins G and M (IgG and IgM) in HIV positive individuals in relation to their cluster of differentiation 4 (CD4) cells count. IgG, IgM and CD4 were assayed using enzyme immunoassay (EIA) and flowcytometry respectively. 341 HIV positive individuals were studied in the present research, 30 (8.7%) of them had T. gondii IgG and IgM, 297 subjects had CD4 cells count a range of 200-400 cells/μL, 27 (9.7%) and 2 (0.6%) of which had T. gondii IgG and IgM respectively. Of the 44 HIV positive subjects with CD4 〉 400 cells/μL, one (2.2%) was positive for T. gondii IgG. In the control group, all the 177 had CD4 〉 400 cells/μL of which, one (0.5%) had T. gondii IgG. The prevalence of T. gondii infection was significantly higher in HIV positive individuals than in controls (P 〈 0.05). Male subjects in the age bracket 18-30 years had significantly higher prevalence when compared to other groups (P 〈 0.05). Although the present findings revealed a low prevalence of T. gondii antibodies in HIV infection, this suggests that a differential toxoplasmosis diagnosis is also necessary in cases of encephalitis in HIV infection.展开更多
Introduction: Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect any warm blood vertebrae, and if first trimester pregnant woman infected, it may cause abortion. The objective is to prov...Introduction: Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect any warm blood vertebrae, and if first trimester pregnant woman infected, it may cause abortion. The objective is to prove the effect of the Toxoplasma gondii concentration in anti-toxoplasma IgG-IgM antibody levels, and the outcomes of Balb/c mice pregnancies. Materials and Methods: The study was conducted in Balb/c mice with inclusion criteria, and was conditioned pregnant. The pathogen strains of Toxoplasma gondii tachyzoite injected intraperitoneally. The blood samples were taken serially to be tested for anti-toxoplasma IgG-IgM antibody levels. After the mice were injected with tachyzoite, they are assessed every day to observe their body weight, vaginal bleeding, and labor. Anti-toxoplasma IgG-IgM antibody levels examined using qualitative mouse IgG-IgM antibody ELISA KIT. Results: Anti-toxoplasma IgM antibody levels increased significantly after 24 hours of injection tachyzoites in all dose groups, and remained high through day 21. Anti-toxoplasma antibody IgG levels increased significantly after 72 hours post injection and remained elevated until day 21. The incidence of abortion is 100% in mice which injected tachyzoite levels 1 × 103 and 1 × 104, and the incidence of abortion approximately 2 - 4 days post injection. 100% of mice that were injected with tachyzoites 1 × 101 and 1 × 102 have labor at term. Physical anomaly was found in baby mice from mice that were injected with tachyzoite 1 × 102. Conclusion: There is a significant correlation between the concentrations of Toxoplasma gondii tachyzoite with anti-toxoplasma IgG-IgM antibody levels, and there is a significant relationship between the concentrations of tachyzoite with abortion.展开更多
Background: Toxoplasma gondii (T. gondii) is an intracellular parasite mainly found in the central nervous system CNS, however, it can persist in multiple tissues in the body. Moreover, T. gondii is the commonest prot...Background: Toxoplasma gondii (T. gondii) is an intracellular parasite mainly found in the central nervous system CNS, however, it can persist in multiple tissues in the body. Moreover, T. gondii is the commonest protozoans causing infections among individuals with acquired immunodeficiency syndrome (AIDS). Thus, the aim of this study was to investigate the frequency of T. gondii infection among AIDS patients in Makkah at Saudi Arabia. Methods: Fifty patients with AIDS proved to be positive by ULTRA HIV Ag-Ab Enzyme Immunoassay, and thirty healthy volunteers negative for AIDS by ULTRA HIV Ag-Ab Enzyme Immunoassay were subjected to determination of anti T. gondii immunoglobulin M (IgM) antibody seropositivity and anti T. gondii immunoglobulin G (IgG) antibody seropositivity using commercially available enzyme-linked immunosorbent assay kits. Results: The results showed that the seropositivity rate of anti T. gondii IgM antibodies among AIDS patients (18%) was significantly higher than in the healthy volunteers group (3.33%). Regarding the serum level of anti T. gondii IgG antibodies among AIDS patients, it was 30% significantly higher compared with those of the seropositive healthy volunteers (6.67%). Conclusions: These statistically significant results support the association between T. gondii infection and AIDS and suggest the usefulness of providing data for an educational program that will be designed to prevent T. gondii infection among AIDS patients.展开更多
文摘Several researchers have investigated the association of numerous opportunistic pathogens with HIV, little is documented on its association with T. gondii in our environment. We investigated the prevalence of T. gondii immunoglobulins G and M (IgG and IgM) in HIV positive individuals in relation to their cluster of differentiation 4 (CD4) cells count. IgG, IgM and CD4 were assayed using enzyme immunoassay (EIA) and flowcytometry respectively. 341 HIV positive individuals were studied in the present research, 30 (8.7%) of them had T. gondii IgG and IgM, 297 subjects had CD4 cells count a range of 200-400 cells/μL, 27 (9.7%) and 2 (0.6%) of which had T. gondii IgG and IgM respectively. Of the 44 HIV positive subjects with CD4 〉 400 cells/μL, one (2.2%) was positive for T. gondii IgG. In the control group, all the 177 had CD4 〉 400 cells/μL of which, one (0.5%) had T. gondii IgG. The prevalence of T. gondii infection was significantly higher in HIV positive individuals than in controls (P 〈 0.05). Male subjects in the age bracket 18-30 years had significantly higher prevalence when compared to other groups (P 〈 0.05). Although the present findings revealed a low prevalence of T. gondii antibodies in HIV infection, this suggests that a differential toxoplasmosis diagnosis is also necessary in cases of encephalitis in HIV infection.
文摘Introduction: Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect any warm blood vertebrae, and if first trimester pregnant woman infected, it may cause abortion. The objective is to prove the effect of the Toxoplasma gondii concentration in anti-toxoplasma IgG-IgM antibody levels, and the outcomes of Balb/c mice pregnancies. Materials and Methods: The study was conducted in Balb/c mice with inclusion criteria, and was conditioned pregnant. The pathogen strains of Toxoplasma gondii tachyzoite injected intraperitoneally. The blood samples were taken serially to be tested for anti-toxoplasma IgG-IgM antibody levels. After the mice were injected with tachyzoite, they are assessed every day to observe their body weight, vaginal bleeding, and labor. Anti-toxoplasma IgG-IgM antibody levels examined using qualitative mouse IgG-IgM antibody ELISA KIT. Results: Anti-toxoplasma IgM antibody levels increased significantly after 24 hours of injection tachyzoites in all dose groups, and remained high through day 21. Anti-toxoplasma antibody IgG levels increased significantly after 72 hours post injection and remained elevated until day 21. The incidence of abortion is 100% in mice which injected tachyzoite levels 1 × 103 and 1 × 104, and the incidence of abortion approximately 2 - 4 days post injection. 100% of mice that were injected with tachyzoites 1 × 101 and 1 × 102 have labor at term. Physical anomaly was found in baby mice from mice that were injected with tachyzoite 1 × 102. Conclusion: There is a significant correlation between the concentrations of Toxoplasma gondii tachyzoite with anti-toxoplasma IgG-IgM antibody levels, and there is a significant relationship between the concentrations of tachyzoite with abortion.
文摘Background: Toxoplasma gondii (T. gondii) is an intracellular parasite mainly found in the central nervous system CNS, however, it can persist in multiple tissues in the body. Moreover, T. gondii is the commonest protozoans causing infections among individuals with acquired immunodeficiency syndrome (AIDS). Thus, the aim of this study was to investigate the frequency of T. gondii infection among AIDS patients in Makkah at Saudi Arabia. Methods: Fifty patients with AIDS proved to be positive by ULTRA HIV Ag-Ab Enzyme Immunoassay, and thirty healthy volunteers negative for AIDS by ULTRA HIV Ag-Ab Enzyme Immunoassay were subjected to determination of anti T. gondii immunoglobulin M (IgM) antibody seropositivity and anti T. gondii immunoglobulin G (IgG) antibody seropositivity using commercially available enzyme-linked immunosorbent assay kits. Results: The results showed that the seropositivity rate of anti T. gondii IgM antibodies among AIDS patients (18%) was significantly higher than in the healthy volunteers group (3.33%). Regarding the serum level of anti T. gondii IgG antibodies among AIDS patients, it was 30% significantly higher compared with those of the seropositive healthy volunteers (6.67%). Conclusions: These statistically significant results support the association between T. gondii infection and AIDS and suggest the usefulness of providing data for an educational program that will be designed to prevent T. gondii infection among AIDS patients.