目的应用生物信息学的方法探讨肿瘤蛋白D52(tumor protein D52,TPD52)和肿瘤蛋白D52样2(tumor protein D52-like 2,TPD52L2)在胃癌中异常表达的临床病理特征,并分析相关基因共表达的意义。方法基于癌症基因组图谱(The Cancer Genome AHa...目的应用生物信息学的方法探讨肿瘤蛋白D52(tumor protein D52,TPD52)和肿瘤蛋白D52样2(tumor protein D52-like 2,TPD52L2)在胃癌中异常表达的临床病理特征,并分析相关基因共表达的意义。方法基于癌症基因组图谱(The Cancer Genome AHas,TCGA)获取数据,利用R语言软件包比较TPD52和TPD52L2在胃癌组织和癌旁组织中的差异表达与临床病理特征的相关性,并探索与免疫细胞浸润水平的情况,Kaplan-Meier生存分析和Cox回归评估TPD52和TPD52L2的表达与患者预后的关系。利用Human Protein Atlas数据库分析TPD52和TPD52L2在胃癌组织中的免疫组化染色情况。借助cBioPortal数据库分析TPD52和TPD52L2的共表达基因,并使用SangerBox平台进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析。通过STRING数据库对共表达基因建立蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,使用Cytoscape 3.9筛选核心共表达基因,并进一步验证核心共表达基因的生存期和相关性。结果胃癌组织中TPD52和TPD52L2的表达水平均明显高于癌旁组织,并与病理分期、组织学分级、幽门螺旋杆菌感染和Barretts食管密切相关。TPD52的表达与Th2和NK细胞等细胞的浸润丰度相关,TPD52L2的表达与Th2和肥大细胞等细胞的浸润丰度相关。与TPD52L2高表达组相比,低表达组有更长的总生存期(OS)、首次进展生存期(FP)和再次进展生存期(PPS)。单因素Cox分析结果表明,TPD52和TPD52L2的表达与TNM分期、病理分期及胃癌患者的预后不良相关,多因素Cox分析结果表明,M分期是影响胃癌患者OS的独立危险因素。KEGG富集分析发现TPD52的共表达基因参与了代谢通路和癌症的转录失调等信号通路,TPD52L2的共表达基因参与了癌症通路和剪接体等信号通路。PPI网络中核心基因生存分析结果表明,高表达组的PLCG1、PRKACA、MAPK11、NGFR和HSP90AB1预后较差,相关性分析发现MAPK11、CYCS和NGFR是与TPD52关系最紧密的基因,RUVBL1、NOP56、HSP90AB1和CCT6A是与TPD52L2关系最紧密的基因,可作为探讨TPD52和TPD52L2参与胃癌恶性生物学行为的候选基因。结论TPD52和TPD52L2在胃癌组织中高表达,且在胃癌的发生发展中发挥着重要作用,有望成为胃癌早期诊断和预后的生物标志物和治疗靶点。展开更多
Seven transition metal molybdovanadoarsenic heteropoly compounds have been synthesized and characterize d using IR,UV,TG-DTA,pH potential ti tration techniques￡(r)The molecular f ormulae of these compounds are pro-po...Seven transition metal molybdovanadoarsenic heteropoly compounds have been synthesized and characterize d using IR,UV,TG-DTA,pH potential ti tration techniques￡(r)The molecular f ormulae of these compounds are pro-posed to be HxMyAsMO10V2O40·zH2 O￡¨x￡1/21?￡3￡?y￡1/2￡±￡?￡2￡?M￡1/2Cr￡?Mn￡?Fe￡?Co￡?Ni￡?Cu￡?Zn￡(c)￡(r)They are all keggin structure￡(r)Surface nature of these compounds have been investigated by temperature programmed desorption and temperature programmed reducti on techniques￡(r)NH 3 -TPD results show that in TPD profile s of the compounds there are two desorption peaks corre sponding to weak acid sites of desorp tion,respectively￡(r)Desorbing activativon energy and preexponential factor of weak acid site of desorption for comp ounds have been calculated￡(r)H2 -TPR re-sults show that introducting transtion metal to molybdovanarsenic acid,the reduction peak temperatures of H2 -TPR shift regularly with increasing d electron numbers of transition metals￡(r)In addition,Zn and Cu heteropoly com-pounds have yet apparent effect of hydrogen spill over￡(r)展开更多
Pt Sn/MgAl 2O 4 catalysts prepared by co impregnation with nominal 0 35% platinum and varying content of tin (0～2 5%) were characterized by temperature programmed reduction (TPR) and temperature programmed desorption...Pt Sn/MgAl 2O 4 catalysts prepared by co impregnation with nominal 0 35% platinum and varying content of tin (0～2 5%) were characterized by temperature programmed reduction (TPR) and temperature programmed desorption of hydrogen (H 2 TPD). TPR results showed that platinum catalyzed the reduction of tin. However, the average oxidation state of tin after reduction depended upon the concentration of tin on the catalysts. H 2 TPD data indicated that tin addition inhibited the inactivated adsorption of hydrogen but promoted the activated adsorption, implying that tin modified both the ensembles of platinum and metal support interface, thus increasing hydrogen mobility and promoting hydrogen spillover.展开更多
A series of ultrafine Mo Co K catalysts with different Co/Mo ratios but constant potassium content were characterized by TPD technique and their catalytic performance for higher alcohol synthesis was investigated The ...A series of ultrafine Mo Co K catalysts with different Co/Mo ratios but constant potassium content were characterized by TPD technique and their catalytic performance for higher alcohol synthesis was investigated The results indicated that there are two kinds of adsorption sites, ie, low temperature adsorption sites (LTAS) around 90?℃ and high temperature adsorption sites (HTAS) above 400?℃ for H 2 and CO on the surface of the catalysts The desorption temperature of HTAS for H 2 and CO as well as the desorption amount of HTAS for H 2 were strongly influenced by the Co/Mo ratio of the catalysts It was found that the addition of Co into the ultrafine Mo K catalyst resulted in a decrease of the HTAS desorption temperature and an increase of the HTAS desorption amount for H 2 As the Co/Mo ratio increased further, the HTAS desorption amount for H 2 increased accordingly while the HTAS desorption temperature remained constant However, the HTAS desorption temperature for CO was found to be mainly influenced by the Co/Mo ratio, and the HTAS desorption temperature got its minimum at the Co/Mo ratio of 1∶7 It was found that a weaker adsorption of H 2 and CO on the HTAS and an appropriate H 2 adsorption amount were favourable for the formation of higher展开更多
文摘目的应用生物信息学的方法探讨肿瘤蛋白D52(tumor protein D52,TPD52)和肿瘤蛋白D52样2(tumor protein D52-like 2,TPD52L2)在胃癌中异常表达的临床病理特征,并分析相关基因共表达的意义。方法基于癌症基因组图谱(The Cancer Genome AHas,TCGA)获取数据,利用R语言软件包比较TPD52和TPD52L2在胃癌组织和癌旁组织中的差异表达与临床病理特征的相关性,并探索与免疫细胞浸润水平的情况,Kaplan-Meier生存分析和Cox回归评估TPD52和TPD52L2的表达与患者预后的关系。利用Human Protein Atlas数据库分析TPD52和TPD52L2在胃癌组织中的免疫组化染色情况。借助cBioPortal数据库分析TPD52和TPD52L2的共表达基因,并使用SangerBox平台进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析。通过STRING数据库对共表达基因建立蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,使用Cytoscape 3.9筛选核心共表达基因,并进一步验证核心共表达基因的生存期和相关性。结果胃癌组织中TPD52和TPD52L2的表达水平均明显高于癌旁组织,并与病理分期、组织学分级、幽门螺旋杆菌感染和Barretts食管密切相关。TPD52的表达与Th2和NK细胞等细胞的浸润丰度相关,TPD52L2的表达与Th2和肥大细胞等细胞的浸润丰度相关。与TPD52L2高表达组相比,低表达组有更长的总生存期(OS)、首次进展生存期(FP)和再次进展生存期(PPS)。单因素Cox分析结果表明,TPD52和TPD52L2的表达与TNM分期、病理分期及胃癌患者的预后不良相关,多因素Cox分析结果表明,M分期是影响胃癌患者OS的独立危险因素。KEGG富集分析发现TPD52的共表达基因参与了代谢通路和癌症的转录失调等信号通路,TPD52L2的共表达基因参与了癌症通路和剪接体等信号通路。PPI网络中核心基因生存分析结果表明,高表达组的PLCG1、PRKACA、MAPK11、NGFR和HSP90AB1预后较差,相关性分析发现MAPK11、CYCS和NGFR是与TPD52关系最紧密的基因,RUVBL1、NOP56、HSP90AB1和CCT6A是与TPD52L2关系最紧密的基因,可作为探讨TPD52和TPD52L2参与胃癌恶性生物学行为的候选基因。结论TPD52和TPD52L2在胃癌组织中高表达,且在胃癌的发生发展中发挥着重要作用,有望成为胃癌早期诊断和预后的生物标志物和治疗靶点。
文摘Seven transition metal molybdovanadoarsenic heteropoly compounds have been synthesized and characterize d using IR,UV,TG-DTA,pH potential ti tration techniques￡(r)The molecular f ormulae of these compounds are pro-posed to be HxMyAsMO10V2O40·zH2 O￡¨x￡1/21?￡3￡?y￡1/2￡±￡?￡2￡?M￡1/2Cr￡?Mn￡?Fe￡?Co￡?Ni￡?Cu￡?Zn￡(c)￡(r)They are all keggin structure￡(r)Surface nature of these compounds have been investigated by temperature programmed desorption and temperature programmed reducti on techniques￡(r)NH 3 -TPD results show that in TPD profile s of the compounds there are two desorption peaks corre sponding to weak acid sites of desorp tion,respectively￡(r)Desorbing activativon energy and preexponential factor of weak acid site of desorption for comp ounds have been calculated￡(r)H2 -TPR re-sults show that introducting transtion metal to molybdovanarsenic acid,the reduction peak temperatures of H2 -TPR shift regularly with increasing d electron numbers of transition metals￡(r)In addition,Zn and Cu heteropoly com-pounds have yet apparent effect of hydrogen spill over￡(r)
文摘Pt Sn/MgAl 2O 4 catalysts prepared by co impregnation with nominal 0 35% platinum and varying content of tin (0～2 5%) were characterized by temperature programmed reduction (TPR) and temperature programmed desorption of hydrogen (H 2 TPD). TPR results showed that platinum catalyzed the reduction of tin. However, the average oxidation state of tin after reduction depended upon the concentration of tin on the catalysts. H 2 TPD data indicated that tin addition inhibited the inactivated adsorption of hydrogen but promoted the activated adsorption, implying that tin modified both the ensembles of platinum and metal support interface, thus increasing hydrogen mobility and promoting hydrogen spillover.
文摘A series of ultrafine Mo Co K catalysts with different Co/Mo ratios but constant potassium content were characterized by TPD technique and their catalytic performance for higher alcohol synthesis was investigated The results indicated that there are two kinds of adsorption sites, ie, low temperature adsorption sites (LTAS) around 90?℃ and high temperature adsorption sites (HTAS) above 400?℃ for H 2 and CO on the surface of the catalysts The desorption temperature of HTAS for H 2 and CO as well as the desorption amount of HTAS for H 2 were strongly influenced by the Co/Mo ratio of the catalysts It was found that the addition of Co into the ultrafine Mo K catalyst resulted in a decrease of the HTAS desorption temperature and an increase of the HTAS desorption amount for H 2 As the Co/Mo ratio increased further, the HTAS desorption amount for H 2 increased accordingly while the HTAS desorption temperature remained constant However, the HTAS desorption temperature for CO was found to be mainly influenced by the Co/Mo ratio, and the HTAS desorption temperature got its minimum at the Co/Mo ratio of 1∶7 It was found that a weaker adsorption of H 2 and CO on the HTAS and an appropriate H 2 adsorption amount were favourable for the formation of higher
