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Temporal and spatial changes in Ca^(2+) distribution during the programmed cell death of tracheary elements 被引量:1
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作者 ZHANG Zongshen, LI Rongqian & WANG JianboKey Laboratory of Ministry of the Education for Plant Developmental Biology, Wuhan University, Wuhan 430072, China 《Chinese Science Bulletin》 SCIE EI CAS 2001年第22期1893-1896,共4页
The changes in Ca2+ distribution in the tra-cheary elements (TEs) of the pepper leaves were studied using the cytochemical method of potassium antimonate. At the early stage of TEs formation, the vacuole and the nucle... The changes in Ca2+ distribution in the tra-cheary elements (TEs) of the pepper leaves were studied using the cytochemical method of potassium antimonate. At the early stage of TEs formation, the vacuole and the nucleus held large volume, and antimonate Ca2+ deposits were observed mainly in the intercellular space and the cell wall. As the thickening of secondary wall occurred, the vacuole, nucleus and other organelles began to rupture, concomitant with the increase of calcium deposits in the cytosol, showing the influx of Ca2+ into the cell. With the further rupture of cytoplasm and other organelles, the number of calcium deposits at the non-thickening cell wall increased, but declined at the thickening bands of the secondary wall. When the cytoplasmic contents disappeared completely, the level of Ca2+ decreased at the non-thickening wall, but by contrast, increased at the thickening bands of the secondary wall. These observations indicated that the dynamic changes in Ca2+ distribution spatially and 展开更多
关键词 PEPPER tracheary elements programmed cell DEATH calcium.
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A Novel System for Xylem Cell Differentiation in Arabidopsis thaliana 被引量:4
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作者 Yuki Kondo Takashi Fujita +1 位作者 Munetaka Sugiyama Hiroo Fukuda 《Molecular Plant》 SCIE CAS CSCD 2015年第4期612-621,共10页
During vascular development, procambial and cambial cells give rise to xylem and phloem cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular development in ... During vascular development, procambial and cambial cells give rise to xylem and phloem cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular development in detail. Here, we establish a novel in vitro experimental system in which vascular development is induced in Arabidopsis thaliana leaf-disk cultures using bikinin, an inhibitor of glycogen synthase kinase 3 proteins. Transcriptome analysis reveals that mesophyll cells in leaf disks synchronously turn into procambial cells and then differentiate into tracheary elements. Leaf-disk cultures from plants expressing the procambial cell markers TDRpro:GUS and TDRpro:YFP can be used for spatiotemporal visualization of procambial cell formation. Further analysis with the tdr mutant and TDIF (tracheary element differentiation inhibitory factor) indicates that the key signaling TDIF-TDR-GSK3s regulates xylem differentiation in leaf-disk cultures. This new culture system can be combined with analysis using the rich material resources for Arabidopsis including cell-marker lines and mutants, thus offering a powerful tool for analyzing xylem cell differentiation. 展开更多
关键词 Arabidopsis thaliana bikinin leaf-disk culture TRANSCRIPTOME tracheary element differentiation signaling
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