The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells.However,most current plasma trackers are not suitable for tracking plasma membranes for a ...The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells.However,most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time.Herein,Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time.This tracker is composed of a lipophilic boron-dipyrromethene(BODIPY)derivative and a hydrophilic zwitterion to form an amphiphilic structure,which enables its targeting ability toward cell membranes.Moreover,a reactive ester group is included to bind with proteins through covalent bonds in cell membranes nonspecifically,which extends retention time in cell membranes.Mem580-F-Sulfo shows intense brightness(94600),with a high molar absorption coefficient of up to about 100000 L·mol^(-1)·cm^(-1)and a fluorescence quantum yield of up to 0.97.It shows fast cell membrane targeting ability and long retention up to 90 min.In brief,this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.展开更多
Modelization equations of six approaches for tracking the sun are recalled and used to evaluate the constraints and performances to which they lead to.The geographical study case is taken for the specific latitude of ...Modelization equations of six approaches for tracking the sun are recalled and used to evaluate the constraints and performances to which they lead to.The geographical study case is taken for the specific latitude of 12 North that is a good matching with the location of the country of Burkina Faso.Three decisive periods were locally established in order to consider the different travels of the sun on sky during one year.This work presents some technical data which facilitates the choice of sun tracking approaches with concern of a concentrator limits such as its angle of acceptance,its motion control card interpolation model,or its minimum irradiation level for energy conversion effectiveness.展开更多
基金supported by the National Natural Science Foundation of China(22278059,22174009,and 22078047)Fundamental Research Funds for the Central Universities(DUT22LAB601 and DUT22LAB608)。
文摘The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells.However,most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time.Herein,Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time.This tracker is composed of a lipophilic boron-dipyrromethene(BODIPY)derivative and a hydrophilic zwitterion to form an amphiphilic structure,which enables its targeting ability toward cell membranes.Moreover,a reactive ester group is included to bind with proteins through covalent bonds in cell membranes nonspecifically,which extends retention time in cell membranes.Mem580-F-Sulfo shows intense brightness(94600),with a high molar absorption coefficient of up to about 100000 L·mol^(-1)·cm^(-1)and a fluorescence quantum yield of up to 0.97.It shows fast cell membrane targeting ability and long retention up to 90 min.In brief,this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.
文摘Modelization equations of six approaches for tracking the sun are recalled and used to evaluate the constraints and performances to which they lead to.The geographical study case is taken for the specific latitude of 12 North that is a good matching with the location of the country of Burkina Faso.Three decisive periods were locally established in order to consider the different travels of the sun on sky during one year.This work presents some technical data which facilitates the choice of sun tracking approaches with concern of a concentrator limits such as its angle of acceptance,its motion control card interpolation model,or its minimum irradiation level for energy conversion effectiveness.
