Tanshinone ⅡA, extracted from Salvia miltiorrhiza Bunge, exerts neuroprotective effects through its anti-inflammatory, anti-oxidative and anti-apoptotic properties. This study intravenously injected tanshinone ⅡA 20...Tanshinone ⅡA, extracted from Salvia miltiorrhiza Bunge, exerts neuroprotective effects through its anti-inflammatory, anti-oxidative and anti-apoptotic properties. This study intravenously injected tanshinone ⅡA 20 mg/kg into rat models of spinal cord injury for 7 consecutive days. Results showed that tanshinone ⅡA could reduce the inflammation, edema as well as compensatory thickening of the bladder tissue, improve urodynamic parameters, attenuate secondary injury, and promote spinal cord regeneration. The number of hypertrophic and apoptotic dorsal root ganglion（L6–S1） cells was less after treatment with tanshinone ⅡA. The effects of tanshinone ⅡA were similar to intravenous injection of 30 mg/kg methylprednisolone. These findings suggested that tanshinone ⅡA improved functional recovery after spinal cord injury-induced lower urinary tract dysfunction by remodeling the spinal pathway involved in lower urinary tract control.展开更多
Sensory information arising from somatic and visceral area is thought to be respectively transmitted to the brain by two distinct sensory neuronal systems, the somatic and visceral sensory pathway. A novel and unique ...Sensory information arising from somatic and visceral area is thought to be respectively transmitted to the brain by two distinct sensory neuronal systems, the somatic and visceral sensory pathway. A novel and unique spinal sensory system, the spinosolitary tract-dorsal column postsynaptic neuronal system (SST-DCPS), was physiologically and anatomically identified. The spinal neurons project to both visceral (the solitary tract nucleus) and somatic sensory (the dorsal column nuclei) nuclei via their branched axons and receive both visceral and somatic sensory information ascending from periphery through dichotomized primary afferents and descending from their targets via their branched axons. The brain might thus be aware of both visceral and somatic sensation via a single SST-DCPS neuronal channel. The finding of SST-DCPS system, as an example, might be considered as an intersection or fuzzy set of the SST and DCPS system and the concept of dichotomy in classification of neurons and neuronal pathways展开更多
文摘为探讨神经生长因子(NGF)在哮喘发病中的作用及其神经免疫调节机制,本研究应用免疫组织化学方法检测各组豚鼠下呼吸道和内脏感觉传入部位白介素-1β(IL-1β)免疫反应的变化.用Western blot方法分别检测各组豚鼠肺、C7～T5段脊神经节和相应节段脊髓背角NGF和IL-1β的表达.用Luzex-F实时图像分析系统和凝胶成像分析系统分别对结果进行图像分析.免疫组织化学结果显示(染色反应强度用灰度值表示):生理盐水组与单纯致敏组豚鼠IL-1β阳性免疫反应产物的平均灰度值没有显著差异(P＞0.05);与这两个对照组相比,哮喘豚鼠气道上皮、肺内炎性细胞、血管平滑肌内的神经末梢、C7～T5段脊神经节及相应节段脊髓背角内IL-1β阳性反应产物的平均灰度值均明显降低(P＜0.01);在哮喘+NGF抗体组(鼻腔吸入NGF抗体),上述部位内IL-1β阳性反应产物的平均灰度值均明显高于哮喘组(P＜0.01).Western blot结果显示:与生理盐水组和单纯致敏组比较,哮喘组豚鼠肺、C7～T5段脊神经节及相应节段脊髓样品中IL-1β或NGF目标带的IDV(integrated density value)与内参照IDV的比值均明显升高(P＜0.01),而哮喘+NGF抗体组上述部位样品中IL-1β或NGF目标带的IDV与内参照IDV的比值均明显低于哮喘组(P＜0.01),表明NGF可上调IL-1β的表达.这些结果提示NGF诱发IL-1β的表达可能是NGF参与哮喘发病的机制之一,NGF抗体抑制哮喘时下呼吸道和内脏感觉传入部位IL-1β的表达可能是治疗哮喘的新途径.
基金supported by the China Postdoctoral Science Foundation,No.2015M581120
文摘Tanshinone ⅡA, extracted from Salvia miltiorrhiza Bunge, exerts neuroprotective effects through its anti-inflammatory, anti-oxidative and anti-apoptotic properties. This study intravenously injected tanshinone ⅡA 20 mg/kg into rat models of spinal cord injury for 7 consecutive days. Results showed that tanshinone ⅡA could reduce the inflammation, edema as well as compensatory thickening of the bladder tissue, improve urodynamic parameters, attenuate secondary injury, and promote spinal cord regeneration. The number of hypertrophic and apoptotic dorsal root ganglion（L6–S1） cells was less after treatment with tanshinone ⅡA. The effects of tanshinone ⅡA were similar to intravenous injection of 30 mg/kg methylprednisolone. These findings suggested that tanshinone ⅡA improved functional recovery after spinal cord injury-induced lower urinary tract dysfunction by remodeling the spinal pathway involved in lower urinary tract control.
基金This work was supported by the National Natural Science Foundation of China ( Grant No. 39670871 ).
文摘Sensory information arising from somatic and visceral area is thought to be respectively transmitted to the brain by two distinct sensory neuronal systems, the somatic and visceral sensory pathway. A novel and unique spinal sensory system, the spinosolitary tract-dorsal column postsynaptic neuronal system (SST-DCPS), was physiologically and anatomically identified. The spinal neurons project to both visceral (the solitary tract nucleus) and somatic sensory (the dorsal column nuclei) nuclei via their branched axons and receive both visceral and somatic sensory information ascending from periphery through dichotomized primary afferents and descending from their targets via their branched axons. The brain might thus be aware of both visceral and somatic sensation via a single SST-DCPS neuronal channel. The finding of SST-DCPS system, as an example, might be considered as an intersection or fuzzy set of the SST and DCPS system and the concept of dichotomy in classification of neurons and neuronal pathways
