Buyang Huanwu Decoction fraction extracted from Buyang Huanwu Decoction contains saponins of Astragalus, total paeony glycoside and safflower flavones. The aim of this study was to demonstrate the neuroprotective effe...Buyang Huanwu Decoction fraction extracted from Buyang Huanwu Decoction contains saponins of Astragalus, total paeony glycoside and safflower flavones. The aim of this study was to demonstrate the neuroprotective effect and mechanism of Buyang Huanwu Decoction fraction on ischemic injury both in vivo and in vitro. In vivo experiments showed that 50-200 mg/kg Buyang Huanwu Decoction fraction reduced infarct volume and pathological injury in ischemia/reperfusion rats, markedly inhibited expression of nuclear factor-KB and tumor necrosis factor-a and promoted nestin protein expression in brain tissue. Buyang Huanwu Decoction fraction （200 mg/kg） exhibited significant effects, which were similar to those of 100 mg/kg Ginkgo biloba extract. In vitro experimental results demonstrated that 10-100 mg/L Buyang Huanwu Decoction fraction significantly improved cell viability, decreased the release of lactate dehydrogenase and malondialdehyde levels, and inhibited the rate of apoptosis in HT22 cells following oxygen-glucose deprivation. Buyang Huanwu Decoction fraction （100 mg/L） exhibited significant effects, which were similar to those of 100 mg/L Ginkgo biloba extract. These findings suggest that Buyang Huanwu Decoction fraction may represent a novel, protective strategy against cerebral ischemia/reperfusion injury in rats and oxygen-glucose deprivation-induced damage in HT22 cells in vitro by attenuating the inflammatory response and cellular apoptosis.展开更多
基金supported by a grant from the Major Programs of Anhui Science and Technology Special Funds,No.08010302099the Doctor Funds of Anhui Medical University,No.XJ200813
文摘Buyang Huanwu Decoction fraction extracted from Buyang Huanwu Decoction contains saponins of Astragalus, total paeony glycoside and safflower flavones. The aim of this study was to demonstrate the neuroprotective effect and mechanism of Buyang Huanwu Decoction fraction on ischemic injury both in vivo and in vitro. In vivo experiments showed that 50-200 mg/kg Buyang Huanwu Decoction fraction reduced infarct volume and pathological injury in ischemia/reperfusion rats, markedly inhibited expression of nuclear factor-KB and tumor necrosis factor-a and promoted nestin protein expression in brain tissue. Buyang Huanwu Decoction fraction （200 mg/kg） exhibited significant effects, which were similar to those of 100 mg/kg Ginkgo biloba extract. In vitro experimental results demonstrated that 10-100 mg/L Buyang Huanwu Decoction fraction significantly improved cell viability, decreased the release of lactate dehydrogenase and malondialdehyde levels, and inhibited the rate of apoptosis in HT22 cells following oxygen-glucose deprivation. Buyang Huanwu Decoction fraction （100 mg/L） exhibited significant effects, which were similar to those of 100 mg/L Ginkgo biloba extract. These findings suggest that Buyang Huanwu Decoction fraction may represent a novel, protective strategy against cerebral ischemia/reperfusion injury in rats and oxygen-glucose deprivation-induced damage in HT22 cells in vitro by attenuating the inflammatory response and cellular apoptosis.
文摘目的研究辛前甘桔汤对慢性鼻窦炎(CRS)小鼠长链非编码RNA小核仁RNA宿主基因16(lnc RNA SNHG16)、表皮生长因子受体(EGFR)及黏蛋白5AC(MUC5AC)m RNA表达的影响。方法36只C57BL/6小鼠随机分为正常组、模型组、羧甲司坦组及辛前甘桔汤低、中、高剂量组,每组6只。采用金黄色葡萄球菌鼻腔灌注建立小鼠CRS模型。正常组与模型组小鼠灌胃质量分数为0.9%的氯化钠溶液(0.6 g·kg^(-1)·d^(-1)),辛前甘桔汤低、中、高剂量组分别灌胃辛前甘桔汤水煎液(0.3、0.6、1.2 g·kg^(-1)·d^(-1)),羧甲司坦组灌胃羧甲司坦口服液(0.3 m L·kg^(-1)·d^(-1)),连续干预14 d。采用苏木精-伊红(HE)及阿尔新蓝-过碘酸雪夫(AB-PAS)染色法观察小鼠鼻黏膜结构,免疫组织化学法观察小鼠鼻黏膜EGFR、MUC5AC表达并测量光密度值;酶联免疫吸附试验(ELISA)检测样本中白介素-6(IL-6)、白介素-8(IL-8)、基质金属蛋白酶-9(MMP-9)水平。实时荧光定量逆转录聚合酶链式反应(RT-q PCR)检测样本中lnc RNA SNHG16、EGFR、MUC5AC m RNA的表达。结果与正常组比较,模型组鼻黏膜上皮结构紊乱,IL-6、IL-8、MMP-9含量明显升高(P<0.05),lnc RNA SNHG16、EGFR、MUC5AC m RNA表达明显升高(P<0.05)。与模型组比较,辛前甘桔汤各剂量组IL-6水平显著降低(P<0.05),辛前甘桔汤中、高剂量组MMP-9、IL-8水平降低(P<0.05),辛前甘桔汤各剂量组lnc RNA SNHG16、EGFR、MUC5AC m RNA表达显著降低(P<0.05)。与羧甲司坦组比较,辛前甘桔汤高剂量组lnc RNA SNHG16、EGFR m RNA表达降低(P<0.05)。结论辛前甘桔汤能降低lnc RNA SNHG16、EGFR、MUC5AC m RNA表达,降低EGFR、MUC5AC蛋白表达,减少IL-6、IL-8、MMP-9表达,进而改善鼻黏膜病理改变。
