PBOND is a web server that predicts the conformation of the peptide bond between any two amino acids. PBOND classifies the peptide bonds into one out of four classes, namely cis imide （cis-Pro）, cis amide （cis-non...PBOND is a web server that predicts the conformation of the peptide bond between any two amino acids. PBOND classifies the peptide bonds into one out of four classes, namely cis imide （cis-Pro）, cis amide （cis-nonPro）, trans imide （trans-Pro） and trans amide （trans-nonPro）. Moreover, for every prediction a reliability index is computed. The underlying structure of the server consists of three stages： （1） feature extraction, （2） feature selection and （3） peptide bond classification. PBOND can handle both single sequences as well as multiple sequences for batch processing. The predictions can either be directly downloaded from the web site or returned via e-mail. The PBOND web server is freely available at http：//195.251.198.21/pbond.html.展开更多
Surgical resection is the preferred option for hepatocellular carcinoma(HCC),but surgical navigation technology using indocyanine green still has some drawbacks such as non-specific imaging,thus it is very important to...Surgical resection is the preferred option for hepatocellular carcinoma(HCC),but surgical navigation technology using indocyanine green still has some drawbacks such as non-specific imaging,thus it is very important to develop newfluorescence imaging technology.All-cis hexaphenyl-1,3-butadiene derivative(ZZ-HPB-NC)with aggregation-induced emission(AIE)feature has been reported to be quickly turned-onfluorescent response in the intraoperative frozen-section slides of HCC.However,the probe did not respond to normal liver tissue around HCC.In order to enhance the diagnostic rate and elucidate the response mechanism,all-trans config-uration EE-HPB-NC,was furtherly synthesized.Within two minutes,non-cancer tissues could befluorescently labeled by EE-HPB-NC by spraying,showing the same effect with ZZ-HPB-NC to HCC.The results indicated that the configuration-induced cross-identificationfluorescence imaging strategy was achieved through the combination of ZZ-and EE-HPB-NC.Then the mechanism of HPB-NC localization in HCC lesions was explored,and the binding of HPB-NC with specific proteins in cells resulted in the AIE effect to label HCC cells.On this basis,the accuracy of specificfluorescence imaging for HCC was further verified on the mouse hepatic neoplasm models,indicating that it has clinical application potential for surgicalfluorescence real-time navigation.展开更多
A series of novel crown aldoxime ethers were synthesized,demonstrating notable thermal and hydrolysis stability.The showcased acid-catalyzed and photo-induced cis/trans isomerization,which enables orthogonal control o...A series of novel crown aldoxime ethers were synthesized,demonstrating notable thermal and hydrolysis stability.The showcased acid-catalyzed and photo-induced cis/trans isomerization,which enables orthogonal control over both guest complexation and the chiroptical effects of these crown aldoxime ethers,manifesting a regulation of complexation through isomerization at binding heteroatoms.展开更多
Ubiquitin (Ub) is an essential modifier conserved in all eukaryotes from yeast to human. Phospholipase A2 activating protein (PLAA), a mammalian homolog of yeast DOA1/UFD3.
文摘PBOND is a web server that predicts the conformation of the peptide bond between any two amino acids. PBOND classifies the peptide bonds into one out of four classes, namely cis imide （cis-Pro）, cis amide （cis-nonPro）, trans imide （trans-Pro） and trans amide （trans-nonPro）. Moreover, for every prediction a reliability index is computed. The underlying structure of the server consists of three stages： （1） feature extraction, （2） feature selection and （3） peptide bond classification. PBOND can handle both single sequences as well as multiple sequences for batch processing. The predictions can either be directly downloaded from the web site or returned via e-mail. The PBOND web server is freely available at http：//195.251.198.21/pbond.html.
基金National Natural Science Foundation of China,Grant/Award Numbers:82172754,81874208Opening Project of Hubei Key Laboratory of Purification and Application of Plant Anti-cancer Active Ingredients,Grant/Award Number:HLPAI2023001。
文摘Surgical resection is the preferred option for hepatocellular carcinoma(HCC),but surgical navigation technology using indocyanine green still has some drawbacks such as non-specific imaging,thus it is very important to develop newfluorescence imaging technology.All-cis hexaphenyl-1,3-butadiene derivative(ZZ-HPB-NC)with aggregation-induced emission(AIE)feature has been reported to be quickly turned-onfluorescent response in the intraoperative frozen-section slides of HCC.However,the probe did not respond to normal liver tissue around HCC.In order to enhance the diagnostic rate and elucidate the response mechanism,all-trans config-uration EE-HPB-NC,was furtherly synthesized.Within two minutes,non-cancer tissues could befluorescently labeled by EE-HPB-NC by spraying,showing the same effect with ZZ-HPB-NC to HCC.The results indicated that the configuration-induced cross-identificationfluorescence imaging strategy was achieved through the combination of ZZ-and EE-HPB-NC.Then the mechanism of HPB-NC localization in HCC lesions was explored,and the binding of HPB-NC with specific proteins in cells resulted in the AIE effect to label HCC cells.On this basis,the accuracy of specificfluorescence imaging for HCC was further verified on the mouse hepatic neoplasm models,indicating that it has clinical application potential for surgicalfluorescence real-time navigation.
基金support of the National Natural Science Foundation of China (Nos.22271201,92056116,22171194,22201194)the Science & Technology Department of Sichuan Province (Nos.2022YFH0095 and 2021ZYD0052)the Fundamental Research Funds for the Central Universities (No.20826041D4117)。
文摘A series of novel crown aldoxime ethers were synthesized,demonstrating notable thermal and hydrolysis stability.The showcased acid-catalyzed and photo-induced cis/trans isomerization,which enables orthogonal control over both guest complexation and the chiroptical effects of these crown aldoxime ethers,manifesting a regulation of complexation through isomerization at binding heteroatoms.
文摘Ubiquitin (Ub) is an essential modifier conserved in all eukaryotes from yeast to human. Phospholipase A2 activating protein (PLAA), a mammalian homolog of yeast DOA1/UFD3.
