The genetic basis for bivalves＇ adaptation and evolution is not well understood. Even few studies have focused on the mechanism of molluscan molecular evolution between the coastal intertidal zone and deep-sea enviro...The genetic basis for bivalves＇ adaptation and evolution is not well understood. Even few studies have focused on the mechanism of molluscan molecular evolution between the coastal intertidal zone and deep-sea environment.In our studies, we first conducted the transcritpome assembly of Modiolus modiolus mussels living in coastal intertidal zones. Also, we conducted transcriptome comparison analyses between M. modiolus and Bathymodiolus platifrons living in hydrothermal vents and cold methane/sulfide-hydrocarbon seeps. De novo assemblies of the clean reads yielded a total of 182 476 and 156 261 transcripts with N50 values of 1 769 and 1 545 in M. modiolus and B. platifrons. A total of 27 868 and 23 588 unigenes were identified, which also displayed the similar GO representation patterns. Among the 10 245 pairs of putative orthologs, we identified 26 protein-coding genes under strong positive selection（Ka/Ks〉1） and 12 genes showing moderate positive selection（0.5展开更多
基金The Strategic Priority Research Program of the Chinese Academy of Sciences under contract No.XDB06010101the Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology under contract No.2015ASKJ02-03+3 种基金Shandong Provincial Natural Science Foundation,China under contract No.ZR2016DQ13the Earmarked Fund for Modern Agro-industry Technology Research System under contract No.CARS-48the Taishan Scholars Climbing Program of Shandongthe project funded by China Postdoctoral Science Foundation
文摘The genetic basis for bivalves＇ adaptation and evolution is not well understood. Even few studies have focused on the mechanism of molluscan molecular evolution between the coastal intertidal zone and deep-sea environment.In our studies, we first conducted the transcritpome assembly of Modiolus modiolus mussels living in coastal intertidal zones. Also, we conducted transcriptome comparison analyses between M. modiolus and Bathymodiolus platifrons living in hydrothermal vents and cold methane/sulfide-hydrocarbon seeps. De novo assemblies of the clean reads yielded a total of 182 476 and 156 261 transcripts with N50 values of 1 769 and 1 545 in M. modiolus and B. platifrons. A total of 27 868 and 23 588 unigenes were identified, which also displayed the similar GO representation patterns. Among the 10 245 pairs of putative orthologs, we identified 26 protein-coding genes under strong positive selection（Ka/Ks〉1） and 12 genes showing moderate positive selection（0.5
