Development of a transient expression system for Panax ginseng based on protoplast isolation from its embryoids

Panax Ginseng(2n=48)represents a quintessential resource in traditional Chinese medicine,renowned for its outstanding medicinal and economic benefits(Choi,2008).But the late start in analyzing the ginseng genome and the poorly developed genetic transformation system still impede the study of ginseng gene function and the application of molecular breeding.Transient transformation has the advantages of high efficiency,low cost,and short cycle while laying the foundation for stable genetic transformation(Chen et al.,2021).In the plant transformation process,the cell wall prevents exogenous DNA or protein entry,significantly reducing the efficiency of the transformation.Protoplasts,as exposed cells wrapped by the plasma membrane,are more likely to absorb exogenous DNA,RNA,and protein.Transgenic systems of protoplasts have been established in several species and applied in many fields,such as gene function research(Gou et al.,2020),gene editing(Yang et al.,2023),and physiological or molecular mechanism research(Aoyagi,2011).For instance,Oryza sativa protoplasts were employed to screen genes involved in rice defense signaling pathways through fluorescent reporter systems,with BiFC employed to verified inter-protein interactions(He et al.,2016).A study transformed Cannabis sativa L.protoplasts with the plasmids carrying GFP and RFP genes,evaluated the efficiency under different transformation conditions by flow cytometry,and verified the induction of synthetic DR5 promoter by IAA based on the constructed system(Beard et al.,2021).

Highly Elastic,Bioresorbable Polymeric Materials for Stretchable,Transient Electronic Systems

Substrates or encapsulants in soft and stretchable formats are key components for transient,bioresorbable electronic systems;however,elastomeric polymers with desired mechanical and biochemical properties are very limited compared to nontransient counterparts.Here,we introduce a bioresorbable elastomer,poly(glycolide-co-ε-caprolactone)(PGCL),that contains excellent material properties including high elongation-at-break(<1300%),resilience and toughness,and tunable dissolution behaviors.Exploitation of PGCLs as polymer matrices,in combination with conducing polymers,yields stretchable,conductive composites for degradable interconnects,sensors,and actuators,which can reliably function under external strains.Integration of device components with wireless modules demonstrates elastic,transient electronic suture system with on-demand drug delivery for rapid recovery of postsurgical wounds in soft,time-dynamic tissues.

Transient Stability Preventive Control of Wind Farm Connected Power System Considering the Uncertainty

To address uncertainty as well as transient stability constraints simultaneously in the preventive control of windfarm systems, a novel three-stage optimization strategy is established in this paper. In the first stage, the probabilisticmulti-objective particle swarm optimization based on the point estimate method is employed to cope with thestochastic factors. The transient security region of the system is accurately ensured by the interior point methodin the second stage. Finally, the verification of the final optimal objectives and satisfied constraints are enforcedin the last stage. Furthermore, the proposed strategy is a general framework that can combine other optimizationalgorithms. The proposed methodology is tested on the modified WSCC 9-bus system and the New England 39-bussystem. The results verify the feasibility of the method.

Migratable Power System Transient Stability AssessmentMethod Based on Improved XGBoost

The data-driven transient stability assessment(TSA)of power systems can predict online real-time prediction by learning the temporal features before and after faults.However,the accuracy of the assessment is limited by the quality of the data and has weak transferability.Based on this,this paper proposes a method for TSA of power systems based on an improved extreme gradient boosting(XGBoost)model.Firstly,the gradient detection method is employed to remove noise interference while maintaining the original time series trend.On this basis,a focal loss function is introduced to guide the training of theXGBoostmodel,enhancing the deep exploration of minority class samples to improve the accuracy of the model evaluation.Furthermore,to improve the generalization ability of the evaluation model,a transfer learning method based on model parameters and sample augmentation is proposed.The simulation analysis on the IEEE 39-bus system demonstrates that the proposed method,compared to the traditional machine learning-based transient stability assessment approach,achieves an average improvement of 2.16%in evaluation accuracy.Specifically,under scenarios involving changes in topology structure and operating conditions,the accuracy is enhanced by 3.65%and 3.11%,respectively.Moreover,the model updating efficiency is enhanced by 14–15 times,indicating the model’s transferable and adaptive capabilities across multiple scenarios.

Improve Strategy for Transient Power Angle Stability Control of VSG Combining Frequency Difference Feedback and Virtual Impedance

As the penetration rate of distributed energy increases,the transient power angle stability problem of the virtual synchronous generator(VSG)has gradually become prominent.In view of the situation that the grid impedance ratio(R/X)is high and affects the transient power angle stability of VSG,this paper proposes a VSG transient power angle stability control strategy based on the combination of frequency difference feedback and virtual impedance.To improve the transient power angle stability of the VSG,a virtual impedance is adopted in the voltage loop to adjust the impedance ratio R/X;and the PI control feedback of the VSG frequency difference is introduced in the reactive powervoltage link of theVSGto enhance the damping effect.Thesecond-orderVSGdynamic nonlinearmodel considering the reactive power-voltage loop is established and the influence of different proportional integral(PI)control parameters on the system balance stability is analyzed.Moreover,the impact of the impedance ratio R/X on the transient power angle stability is presented using the equal area criterion.In the simulations,during the voltage dips with the reduction of R/X from 1.6 to 0.8,Δδ_(1)is reduced from 0.194 rad to 0.072 rad,Δf_(1)is reduced from 0.170 to 0.093 Hz,which shows better transient power angle stability.Simulation results verify that compared with traditional VSG,the proposedmethod can effectively improve the transient power angle stability of the system.

Wireless distributed test system based on transient pressure signal detection and recognition

During the test on transient pressure signal in explosion field,false trigger caused by field interference can lead to test failure.To improve the stability of test system,a signal detection and recognition technology is proposed for transient pressure test system.In the process of signal acquisition,firstly,electrical levels are monitored in real time to find effective abrupt changes and mark them;then the effective data segments are detecdted totected;thus the effective signals can be acquired in turn finally.The experimental results show that the shock wave signal can be collected effectively and the reliability of the test system can be improved after removal of interferences.

Design and implementation of configurable parameters test system for transient pressure measurement

For the shock wave overpressure signal measurement of explosion field, the type and equivalent of the ammunition as well as the distance from test nodes to ground zero have a great influence on the characteristics of shock wave signal. To ensure that shock wave signal with different characteristic parameters can be collected completely in the test of shooting range, a tran- sient pressure signal storage system with configurable parameters is presented. In order to meet the requirements of different test nodes, the system can set parameters of all test nodes by wireless communication in explosion field. The feasibility and re- liability of the system are fully verified through transient pressure measurement in explosion field.

TRANSIENT RESPONSE OF A VALVE CONTROL HYDRAULIC SYSTEM WITHLONG PIPES

The simulation model of a valve control hydraulic system with long pipe isestablished in Simulink4.0, and then the step responses of the systems with difference pipeparameters are investigated by simulation. Simulation results show that the long pipes will slowdown the step response of system and make it fluctuate periodically. The results of simulationconform to the results of experiment on the whole, which proves the mathematic model is correct.

Application of Computational Fluid Dynamics and Fluid Structure Interaction Techniques for Calculating the 3D Transient Flow of Journal Bearings Coupled with Rotor Systems

Journal bearings are important parts to keep the high dynamic performance of rotor machinery. Some methods have already been proposed to analysis the flow field of journal bearings, and in most of these methods simplified physical model and classic Reynolds equation are always applied. While the application of the general computational fluid dynamics （CFD）-fluid structure interaction （FSI） techniques is more beneficial for analysis of the fluid field in a journal bearing when more detailed solutions are needed. This paper deals with the quasi-coupling calculation of transient fluid dynamics of oil film in journal bearings and rotor dynamics with CFD-FSI techniques. The fluid dynamics of oil film is calculated by applying the so-called ＂dynamic mesh＂ technique. A new mesh movement approacb is presented while the dynamic mesh models provided by FLUENT are not suitable for the transient oil flow in journal bearings. The proposed mesh movement approach is based on the structured mesh. When the joumal moves, the movement distance of every grid in the flow field of bearing can be calculated, and then the update of the volume mesh can be handled automatically by user defined function （UDF）. The journal displacement at each time step is obtained by solving the moving equations of the rotor-bearing system under the known oil film force condition. A case study is carried out to calculate the locus of the journal center and pressure distribution of the journal in order to prove the feasibility of this method. The calculating results indicate that the proposed method can predict the transient flow field of a journal bearing in a rotor-bearing system where more realistic models are involved. The presented calculation method provides a basis for studying the nonlinear dynamic behavior of a general rotor-bearing system.

Life Prediction Based on Transient Dynamics Analysis of Van Semi-trailer with Air Suspension System

The early fatigue damage in the van-body of the semi-trailer is often caused by the unique mechanical characteristics and the dynamic impact of the loads.The traditional finite element method with static strength analysis cannot support the fatigue design of van-body;thus,the dynamics analysis should be adopted for the endurance performance.The accurate dynamics model to describe the transient impacts of all kinds of uneven road and the proper system transfer functions to calculate the load transfer effects from tire to van-body are two critical factors for transient dynamics analysis.In order to evaluate the dynamic performance,the dynamics model of the trailer with the air suspension is brought forward.Then the analysis method of the power spectral density （PSD） is set up to study the transient responses of the road dynamic impacts.The transient responses transferred from axles to van-body are calculated,such as dynamic stress,dynamic RMS acceleration,and dynamic load factors.Based on the above dynamic responses,the fatigue life of van-body is predicted with the finite element analysis （FEA） method.Applying the test parameters of the trailer with air suspension,the simulation system with Matlab/Simulink is constructed to describe the dynamic responses of the impacts of the tested PSD of the vehicle axles,and then the fatigue life is predicted with FEA method.The simulated results show that the vibration level of the van-body with air suspension is reduced and the fatigue life is improved.The real vehicle tests on different roads are carried out,and the test results validate the accuracy of the simulation system.The proposed fatigue life prediction method is effective for the virtual design of auto-body.

Complex transient dynamics of hidden attractors in a simple 4D system

A simple four-dimensional system with only one control parameter is proposed in this paper. The novel system has a line or no equilibrium for the global control parameter and exhibits complex transient transition behaviors of hyperchaotic attractors, periodic orbits, and unstable sinks. Especially, for the nonzero-valued control parameter, there exists no equilibrium in the proposed system, leading to the formation of various hidden attractors with complex transient dynamics. The research results indicate that the dynamics of the system shows weak chaotic robustness and depends greatly on the initial states.

Three-dimensional transient numerical simulation for intake process in the engine intake port-valve-cylinder system

This paper presents a KIVA-3 code based numerical model for three-dimensional transient intake flow in the intake port-valve-cylinder system of internal combustion engine using body-fitted technique, which can be used in numerical study on internal combustion engine with vertical and inclined valves, and has higher calculation precision. A numerical simulation (on the intake process of a two-valve engine with a semi-sphere combustion chamber and a radial intake port) is provided for analysis of the velocity field and pressure field of different plane at different crank angles. The results revealed the formation of the tumble motion, the evolution of flow field parameters and the variation of tumble ratios as important information for the design of engine in-take system.

A Nonlinear Coordinated Approach to Enhance the Transient Stability of Wind Energy-Based Power Systems

This paper proposes a novel framework that enables the simultaneous coordination of the controllers of doubly fed induction generators(DFIGs) and synchronous generators(SGs).The proposed coordination approach is based on the zero dynamics method aims at enhancing the transient stability of multi-machine power systems under a wide range of operating conditions. The proposed approach was implemented to the IEEE39-bus power systems. Transient stability margin measured in terms of critical clearing time along with eigenvalue analysis and time domain simulations were considered in the performance assessment. The obtained results were also compared to those achieved using a conventional power system stabilizer/power oscillation(PSS/POD) technique and the interconnection and damping assignment passivity-based controller(IDA-PBC). The performance analysis confirmed the ability of the proposed approach to enhance damping and improve system’s transient stability margin under a wide range of operating conditions.

Transient Properties of a Bistable System Driven by Cross-Correlated Noises:Correlation Times are Nonzero Case

The transient properties of a bistable system driven by cross-correlated noises are investigated,the correlation times of the correlations between the noises are nonzero.The explicit expression of the mean,first-passage time(MFPT)is obtained.From numerical computations we find the following.(1)The MFPT of the system is affected by the correlation time T and the correlation strength λ,T and λ play opposing roles in the MFPT.(2)For the case of perfectly correlated noises(λ=1),the MFPT corresponding to α>D and α<D(α and D are the additive noise and multiplicative noise intensities,respectively)exhibit the same behaviors and the MFPT for α=D is continuous,which is very different from the case of τ=0[Phys.Rev.E 53(1996)5764].

Multistability and coexisting transient chaos in a simple memcapacitive system

The self-excited attractors and hidden attractors in a memcapacitive system which has three elements are studied in this paper.The critical parameter of stable and unstable states is calculated by identifying the eigenvalues of Jacobian matrix.Besides,complex dynamical behaviors are investigated in the system,such as coexisting attractors,hidden attractors,coexisting bifurcation modes,intermittent chaos,and multistability.From the theoretical analyses and numerical simulations,it is found that there are four different kinds of transient transition behaviors in the memcapacitive system.Finally,field programmable gate array(FPGA)is used to implement the proposed chaotic system.

An efficient transient gene expression system for protein subcellular localization assay and genome editing in citrus protoplasts

Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility. The protoplast-based transient gene expression system is a powerful tool for gene functional characterization and CRISPR/Cas9 genome editing in higher plants, but it has not been widely used in citrus. In this study, the polyethylene glycol(PEG)-mediated method was optimized for citrus callus protoplast transfection, with an improved transfection efficiency of 68.4%. Consequently, the efficiency of protein subcellular localization assay was increased to 65.8%, through transient expression of the target gene in protoplasts that stably express the fluorescent organelle marker protein. The gene editing frequencies in citrus callus protoplasts reached 14.2% after transient expression of CRISPR/Cas9 constructs. We demonstrated that the intronic polycistronic tRNAgRNA(inPTG) genome editing construct was functional in both the protoplast transient expression system and epicotyl stable transformation system in citrus. With this optimized protoplast transient expression system, we improved the efficiency of protein subcellular localization assay and developed the genome editing system in callus protoplasts, which provides an approach for prompt test of CRISPR vectors.

Transient Stability Analysis of Power System Based on an Improved Neural Network

A new type of ANN (Artificial Neural Network) structure is introduced, and a nonlinear transformation of the original features is proposed so as to improve the learning covergence of the neural network. This kind of improved ANN is then used to analyse the transient stability of two real power systems. The results show that this method possesses better effectiveness and high convergence speed.

Full electromagnetic transient simulation for large power systems

For building Global Energy Interconnection(GEI), it is necessary to implement new breakthroughs on largepower system simulation. Key routes for implementing full electromagnetic transient simulation of large-power systems are described in this paper, and a top framework is designed. A combination of the new large time step algorithm and the traditional small-time step algorithm is proposed where both parts A and B are calculated independently. The method for integrating the Norton equivalence of the power electronic system to the entire power grid is proposed. A two-level gird division structure is proposed, which executes a multi-rate parallel calculation among subsystems and element parallel calculation in each subsystem. The initialization method of combining load flow derivation and automatic trial-and-error launching is introduced. The feasibility of the method is demonstrated through a practical power grid example, which lays a foundation for further research.

Transient characteristics verification method for DC transformer used in flexible HVDC system

Previous studies have proposed higher requirements for the transient characteristics of a DC transformer used in a flexible high-voltage direct current(HVDC) system to achieve faster sampling speed and meet wider bandwidth requirements of the control and protection signal, and to eventually suppress the large transient fault current. In this study, a transient characteristics verification method is proposed for transient characteristics verification of a DC transformer used in a flexible HVDC system based on resampling technology and LabVIEW measurement technology after analyzing the key technology for transient characteristics verification of a DC transformer. A laboratory experiment for the transient characteristics of a full-fiber electronic DC transformer is conducted, and experimental results show that such verification method can be employed for frequency response and step response verification of a DC transformer at 10% of the rated voltage and current, and can eventually improve the screening of a DC transformer.

Generation of transient chaos from two-dimensional systems via a switching approach

A new approach of generating transient chaos from two-dimensional（2D） continuous autonomous systems within finite time is presented.Based on an absolute-value switching law,the phenomenon of transient chaos takes place by switching between three 2D systems.Basic dynamic behavior of the systems is investigated.Numerical examples illustrate the validity of the results.