Inheritance patterns of secondary symbionts during sexual reproduction of pea aphid biotypes

Herbivorous insects frequently harbor bacterial symbionts that affect their ecol- ogy and evolution. Aphids host the obligatory endosymbiont Buchnera, which is requiredfor reproduction, together with facultative symbionts whose frequencies vary across aphid populations. These maternally transmitted secondary symbionts have been particularlystudied in the pea aphid, Acyrthosiphon pisum, which harbors at least 8 distinct bacterial species （not counting Buchnera） having environmentally dependent effects on host fitness.In particular, these symbiont species are associated with pea aphid populations feeding on specific plants. Although they are maternally inherited, these bacteria are occasionallytransferred across insect lineages. One mechanism of such nonmaternal transfer is paternal transmission to the progeny during sexual reproduction. To date, transmission of secondarysymbionts during sexual reproduction of aphids has been investigated in only a handful of aphid lineages and 3 symbiont species. To better characterize this process, we investigatedinheritance patterns of 7 symbiont species during sexual reproduction of pea aphids through a crossing experiment involving 49 clones belonging to 9 host-specialized biotypes, and117 crosses. Symbiont species in the progeny were detected with diagnostic qualitative PCR at the fundatrix stage hatching from eggs and in later parthenogenetic generations.We found no confirmed case of paternal transmission of symbionts to the progeny, and we observed that maternal transmission of a particular symbiont species （Serratia symbiotica）was quite inefficient. We discuss these observations in respect to the ecology of the pea aphid.

Reliability and safety analysis of redundant vehicle management computer system

Redundant techniques are widely adopted in vehicle management computer （VMC） to ensure that VMC has high reliability and safety. At the same time, it makes VMC have special characteristics, e.g., failure correlation, event simultaneity, and failure self-recovery. Accordingly, the reliability and safety analysis to redundant VMC system （RVMCS） becomes more difficult. Aimed at the difficulties in RVMCS reliability modeling, this paper adopts generalized stochastic Petri nets to establish the reliability and safety models of RVMCS. Then this paper analyzes RVMCS oper- ating states and potential threats to flight control system. It is verified by simulation that the reli- ability of VMC is not the product of hardware reliability and software reliability, and the interactions between hardware and software faults can reduce the real reliability of VMC obviously. Furthermore, the failure undetected states and false alarming states inevitably exist in RVMCS due to the influences of limited fault monitoring coverage and false alarming probability of fault mon- itoring devices （FMD）. RVMCS operating in some failure undetected states will produce fatal threats to the safety of flight control system. RVMCS operating in some false alarming states will reduce utility of RVMCS obviously. The results abstracted in this paper can guide reliable VMC and efficient FMD designs. The methods adopted in this paper can also be used to analyze other intelligent systems＇ reliability.