Distribution Profiles and Interrelations of Stomatal Conductance, Transpiration Rate and Water Dynamics in Young Maize Laminas under Nitrogen Deprivation

Seven-day-old maize (Zea mays) plants were grown hydroponically for ten days in N-deprived nutrient solution. The distribution profiles according to the position on the stem of the –N laminas stomatal conductance, transpiration rate, photosynthetic rate (1st-group) were monitored, along with the corresponding profiles of dry mass, water amount, water content, length, surface area, and specific surface area (2nd-group), relative to control. In the uppermost –N laminas, the changes within a parameter of the 1st-group were significantly higher and of the 2nd-group significantly lower than the control, respectively. Correlations of the corresponding values among the parameters of the 1st-or 2nd-group were linear. The parameters between groups correlated non-linearly. Transpiration rate was divided by the lamina’s dry mass correlated with surface area in a power-type function. The slopes of the response ratios linear relations between the various pairs of parameters could be used for simulation of a lamina’s response to the deprivation.

Comparison of the S-, N- or P-Deprivations’ Impacts on Stomatal Conductance, Transpiration and Photosynthetic Rate of Young Maize Leaves

Seven-day-old maize (Zea mays) plants were grown hydroponically for ten days in deprived nutrient solutions against the corresponding control grown under full nutrition;the effects of S-, N- or P-deprivation on laminas’ mean stomatal conductance (gs), transpiration rate (E) and photosynthetic rate (A) were monitored, along with the impact on the laminas’ total dry mass (DM), water amount (W), length and surface area (Sa). Furthermore, a time series analysis of each parameter’s response ratios (Rr), i.e. the treatment’s value divided by the corresponding control’s one, was performed. Under S-deprivation, the Rr of laminas’ mean gs, E, and A presented oscillations within a ±15% fluctuation zone, notably the “control” zone, whilst those of laminas’ total DM, water amount, surface area, and length included oscillation during the first days and deviation later on, presenting deviation during d10. Under the N-deprivation conditions all Rr time courses except the A one, included early deviations from the control zone without recovering. The deviation from the control zone appeared at d4. Under P-deprivation, all Rr time courses represented oscillations within the control zone. P-deprivation’s patterns resembled those of S-deprivation. Compared to the one of the S-deprivation, the P-one’s oscillations took place within a broader zone. Linear relationships among the various Rr patterns were found between gs-E, gs-A, E-A, DM-W and DM-Sa. In conclusion, the impact of P-deprivation appeared in an early stage and included an alleviation action, the one of N-deprivation appeared early with no alleviation action, whilst that of S-deprivation appeared later, being rather weaker when compared to the impact of the P-deprivation’s impact.

Study on Transpiration and Stomatal Conductance Characteristics of C3 and C4 Plant

The transpiration experiment was done under greenhouse conditions with a C3 plant sweet pepper （Capsicum annuum Linn.） and two C4 plants, sorghum （Sorghum bicolor L.Moench） and maize （Zea mays Linn.）. Three species were irrigated with three different water treatment levels of 100%, 66% and 33% which gave a comparison of tolerance and adaptation to irrigation and two different levels of water stress. The measurements of transpiration rate and stomatal conductance were done between 8.00 a.m. and 16.00 p.m. with measurements about each 1.5 h with an infrared gas analyzer. The results showed that Z. mays probably due to a higher leaf area had very low values and was significantly different （LSD pairwise comparison） from C. annuum and S. bicolor. The hypotheses that C4 plants and C3 plants have different transpiration rates and stomatal conductance could not be shown with the results. However, the hypotheses that for the same species, the highest values in transpiration rate and stomatal conductance were with the 100% irrigation treatment and the lowest values were with the 33% irrigation treatment could be accepted due to the results of this trial.

Growth and Eco-Physiological Performance of Cotton Under Water Stress Conditions

A cotton cultivar Xinluzao 8 was grown under four levels of water stress treatments （normal irrigation, slight, mild and severe water stress） from the initial reproductive growth stage in Shihezi, Xinjiang, China, in 2002, to evaluate the growth and eco-physiological performances. Under water stress conditions, the transpiration ability decreased while the leaf temperature increased. Although the relative leaf water content decreased as water stress increased, the differences among the treatments were small, indicating that cotton has high ability in maintaining water in leaf. The stomatal density increased as water stress increased, while the maximum stomatal aperture reduced only in the severest stressed plants. The time of the maximum stomatal aperture was delayed in the mild and severe stressed plants. When severe stress occurred, the stomata were kept open until the transpiration decreased to nearly zero, suggesting that the stomata might not be the main factor in adjusting transpiration in cotton. Cotton plant has high adaptation ability to water stress conditions because of decrease in both stomatal conductance and hydraulic conductance from soil-to-leaf pathway. The actual quantum yield of photosystem Ⅱ （PS Ⅱ） decreased under water stress conditions, while the maximum quantum yield of PS Ⅱ did not vary among treatments, suggesting that PS II would not be damaged by water stress. The total dry weight reduced as water stress increased.

Towards a more flexible representation of water stress effects in the nonlinear Jarvis model

To better interpret summer maize stomatal conductance （gs） variation under conditions of changing water status at different growth stages, three water stress indicators, soil water content （SWC）, leaf-air temperature difference （AT） and leaf level water stress index （CWSIL） were employed in Jarvis model, which were Js, JT and Jc models respectively. Measurements of gs were conducted in a summer maize field experiment during the year 2012-2013. In the insufficient irrigation exper- iment, three levels of irrigation amount were applied at four different growth stages of summer maize. We constructed three scenarios to evaluate the performance of the three water stress indicators for estimating maize gs in a modified Jarvis model. Results showed that JT and Jc models had better simulation accuracy than the Js model, especially at the late growth stage （Scenario 1） or considering the plant recovery compensation effects （Scenario 2）. Scenario 3 indicated that the more environmental factors were adopted, the better prediction performance would be for Js model. While for JT model, two environmental factors （photosynthesis active radiation （PAR）, and vapor pressure deficit （VPD）） seemed good enough to obtain a reliable simulation. When there were insufficient environmental data, CWSI, would be the best option. This study can be useful to understand the response of plant stomatal to changing water conditions and will further facilitate the application of the Jarvis model in various environments.

Growth and Physiological Performance of Aerobic and Lowland Rice as Affected by Water Stress at Selected Growth Stages

Aerobic rice technology is still new in Malaysia, and information regarding MARDI Aerob 1(MA1), the first local aerobic rice variety, is still lacking. Therefore, comparative studies were carried out to determine the physiological performance of aerobic rice variety MA1 and lowland rice variety MR253 under water stress given at the panicle initiation, flowering and ripening stages. This experiment was arranged in a randomized complete block design. Stomatal conductance(g_s), chlorophyll a fluorescence(F_v/F_m), leaf relative water content(leaf RWC), and soil moisture content(SMC) as well as yield component parameters such as panicle number, grain yield and 100-grain weight were measured. Results revealed that gs and leaf RWC for both varieties decreased with depletion of SMC. The correlation study between the physiological parameters and SMC indicated that F_v/F_m was not affected by water stress, regardless of varieties. The yield components(panicle number, grain yield and 100-grain weight) for both varieties greatly decreased when water stress was imposed at the panicle initiation stage. This study showed that the panicle initiation period was the most sensitive stage to water stress that contributed to a substantial reduction in yield for both varieties. Under the aerobic condition(control), MR253 produced higher panicle number, 100-grain weight and yield than MA1. Although MR253 is bred for lowland, it is well adapted to aerobic condition.

Effects of different inputs of organic matter on the response of plant production to a soil water stress in Sahelian region

The aim was to study the effects of organic management like the application of organic matters on crop production. This research is placed in the context of climate change impact mitigation. A field experiment was conducted during the dry season. Rainfall inputs were simulated by irrigation to study the effects of water stress during the flowering period of a grain on the agronomic and the physiological behavior of the plant. The measurements were made on the volumetric soil moisture, stomatal conductance, and leaf area index (LAI), grain yield, straw and weight of 100 grains. The water use efficiency (WUE) and yield losses were evaluated. The results of the volumetric soil moisture showed that the use of localized input under water stress (STR-T1) recorded the lowest moisture in the surface horizons. Treatment with localized input under water stress with or without fertilization (STR-T1, STR-T1 + N) showed an ability of stomatal regulation compared to the control (STR- T0) and the input application by spreading (STR- T2). (STR-T1 + N) has initiated an early stomatal closure of the plant because of the effect of nitrogen. However, despite a more pronounced water stress with stomatal closure, the LAI and the grain yield were greater with (STR-T1) and (STR-T1 + N). The results showed that the inputs of localized organic fertilization with or without nitrogen grain yields were the highest regardless of the hydric regime applied. However the losses of grain yield were higher in treatments with organic inputs in spreading and localized under water stress. The WUE by the crop was reduced compared to the control with organic inputs under STR. In this study we show that the use of organic matter increases de farmers risk and this notion of risk is high and it is necessary to consider this risk in the proposals of technical innovations.

CO_2, H_2O exchange and stomatal regulation of regenerated Camptotheca acuminata plantlets during ex vitro acclimatization

For finding the changes in CO2, H20 exchange and their stomatal regulation during ex vitro acclimatization of regenerated Camptotheca acuminata plantlets, the net photosynthesis rate （Pn）, respiration rate （Ro）, light compensation point （Lc） and light saturation point （Ls）, transpiration rate （Tr）, stomatal conductance （gs） and water use efficiency（WUE） were measured during 37 days of ex vitro acclimatization. The results showed that Pn sharply increased until 29 days, then slightly decreased. A substantial decrease in Lc and a substantial increase of Ls in the former two weeks were observed, indicating the light regime enlargement for effective leaf photosynthesis. Tr and gs abruptly decreased during the first week then linearly increased until 29days ex vitro acclimatization, reflecting the strong regulation effect of stomata on water changes of ex vitro acclimating plantlets. Stomatal regulation effect on CO2 exchange was different from that on water exchange, i.e. P, was almost independent of gs during the first week, while P. was significantly correlated with gs thereafter （i.e. dual patterns）. Different from dual patterns of gs-Pn relation, the Tr monotonously linearly increased with gs. Furthermore, WUE was almost independent on gs during the first week, while a marked decreasing tendency with gs was found thereafter. At the beginning of the acclimatization, WUE was mainly determined by photosynthetic capacity, while transpiration becomes a main determinant factor for WUE from 7 to 37 days＇ acclimatization.

Physiological Performances of Temperate Vegetables with Response to Chronic and Acute Heat Stress

In face of climate change catastrophes, understanding the thermal limits and optimal physiological thermal window food crop is of particular urgency. This research aims to evaluate: 1) how physiological performances of plant will change with increasing chronic and acute heat stress;2) if the examined parameters form a hierarchy in terms of thermal tolerance;and 3) the optimal thermal window and critical temperatures of the examined plants with response to chronic and acute heat stress. Six temperate vegetables were subjected to chronic and acute heat stress and a suite of physiological parameters were evaluated. Dose responses were observed in shoot fresh weight, photosynthetic gas exchange, photosynthetic oxygen evolution, electron transfer rate, photo- and non-photochemical quenching with significant drop in performance as early as 28°C for selected species. Conversely, ratio of variable to maximum fluorescence (Fv/Fm) was not affected by heat stress until 46°C in chronic heat stress. Examining the temperature at which a measured parameter’s performance dropped by 50% compared to control (LT50), a distinct hierarchy of the indices was observed for Canasta, recombinant inbred line 141, Lactuca serriola and Lactuca sativa (L. “Salinas”): shoot fresh weight, representing the highest integrated level of photosynthesis was the most sensitive to thermal stress (28°C - 30°C), followed by oxygen evolution (35°C - 45°C) while non-photochemical and photochemical quenching which is subcellular function of stress alleviation had a much higher capacity failure temperature (47°C - 60°C). It is expected that Fv/Fm ratio, a measurement of sub-cellular structural integrity, will approach that of non-photochemical and photochemical quenching, if not exceeding it. By examining the photosynthetic parameters via their hierarchy of biological organization, it can be inferred that plants like Arugula and recombinant inbred line 192 are already operating near their thermal limit and have less energetic investment into heat stress mediation whereas L. serriola prioritizes thermal tolerance at the expense of photosynthesis efficiency.

Morphological and physiological responses to drought stress of carob trees in Mediterranean ecosystems

The greatest failure rate of reforestation programs is basically related to water deficit,especially at the seedling stage.Therefore,the main objective of this work is to investigate the responses of three accessions of carob trees(Ceratonia siliqua L.)with 2-year-old from different climate regions to drought generated by four water treatments:Tc(250 mm),T1(180 mm),T2(100 mm),and T3(50 mm).The first accession(A1)comes from the protected national park of Ichkeul in northern Tunisia.This zone belongs to the bioclimatic sub-humid stage.The second accession(A2)comes from Melloulech,located in the center-east of Tunisia,belonging to the bioclimatic semi-arid stage.The third accession(A3)comes from the mountain of Matmata,located in the south of Tunisia,belonging to the bioclimatic hyper-arid stage.The experiment was undertaken in a greenhouse.Gaz exchange indices(net photosynthesis(A),stomatal conductance(gs),transpiration rate(E),and internal CO_(2) concentration(Ci))were determined.Predawn(Ψpd)and midday(Ψmd)leaf water potentials,relative soil water content(SWC),and morphological parameters(plant height(H),number of leaves(NL),number of leaflets(Nl),and number of branches(NB))were estimated.The results showed that significant differences(P<0.001)were found between physiological and morphological parameters of each accession.The highest growth potential was recorded for Tc treatment in both accessions A1 and A2.Significant decreases in gs,E,Ci,and SWC were recorded with the increases in water stress applied from treatment T1 to T3.Positive and significant correlations were found between SWC andΨpd for all studied accessions.Ψpd andΨmd decreased as water stress increased,ranging from–0.96 to–1.50 MPa at sunrise and from–1.94 to–2.83 MPa at midday,respectively,under control and T3 treatments.C.siliqua accessions responded to drought through exhibiting significant changes in their physiological and morphological behavior.Both accessions A1 and A2 showed greater drought tolerance than accession A3.These seedlings exhibit different adaptive mechanisms such as stress avoidance,which are aimed at reducing transpiration,limiting leaf growth,and increasing root growth to exploit more soil water.Therefore,C.siliqua can be recommended for the ecological restoration in Mediterranean ecosystems.

小麦叶片和冠层尺度气体交换模型关键参数的季节变化特征

本文从叶片和冠层尺度入手,采用手持式光合仪和涡动相关法,分析确定C3作物春小麦和冬小麦气体交换模型关键参数随季节变化的特征。结果表明:春小麦叶片尺度最大羧化速率和最大电子传递速率在生育末期快速减小,其他阶段差异不显著,但二者之比随发育期变化相对稳定,均值为2.2;春小麦生长季叶片气孔导度模型斜率在9~11间变动,均值为9.7。冬小麦冠层尺度最大羧化速率和最大电子传递速率随发育期呈抛物线变化,但二者之比相对稳定,均值为1.43;冬小麦冠层导度模型斜率在不同年份不同季节变化无明显规律,整个生育期围绕均值12波动。本研究论证了表征作物光合能力的参数随生育期变化以及气孔导度模型斜率相对稳定的特征。可为陆气相互作用过程分析以及生态系统碳—水循环模拟提供参数化依据。

基于无人机图像纹理和表型参数的夏玉米水分胁迫诊断

农田水分胁迫是影响作物生长发育和产量品质的重要原因。及时准确地诊断作物水分胁迫状况,对于实现精准灌溉、提高作物抗逆性和产量等具有重要意义。为优化夏玉米水分胁迫诊断方法和提高诊断精度,该研究以夏玉米为对象,利用无人机搭载六通道多光谱传感器获取2022年夏玉米拔节期和抽雄期的遥感影像数据,且同步采集夏玉米气孔导度和表型参数数据,监督分类剔除冗余背景后使用灰度共生矩阵计算得到冠层植被指数和图像纹理信息,通过贝叶斯信息准则和全子集筛选法筛选出敏感的植被指数、图像纹理和表型参数及其组合,结合极限学习机、随机森林和反向传播神经网络3种机器学习方法构建夏玉米气孔导度预估模型,并基于最优气孔导度预估模型绘制夏玉米水分胁迫状况反演图。结果表明,多光谱图像的夏玉米冠层反射率与气孔导度呈弱负相关,植被指数和表型参数与气孔导度呈显著正相关,不同波段的图像纹理均与气孔导度有较高的相关性。植被指数用于评估植被整体健康和水分状况,图像纹理用于捕捉作物空间分布、纹理和结构特征,表型参数用于立体反映作物生理和形态信息,它们在诊断作物水分胁迫的机理上具有互补性。基于植被指数、图像纹理和表型参数构建的反向传播神经网络模型是夏玉米水分胁迫诊断的最佳模型(决定系数为0.841,均方根误差为0.043 mol/(m^(2)·s),平均绝对误差为0.034 mol/(m^(2)·s)),并显著改善了对气孔导度较低值的低估情况。绘制的夏玉米水分胁迫状况反演图呈现出广泛的应用潜力,能够便捷准确地诊断作物水分胁迫状况,以优化灌溉策略,调整资源分配。研究结果可为夏玉米的水分胁迫诊断提供一种可行而准确的方法。

Effects of Drought Stress on Photosynthetic Characteristics of Kentucky Bluegrass

Drought stress is one of the major constraints on turf growth and performance in northern China. Based on previous assessments of drought tolerance levels, four varieties of Kentucky bluegrass （Poa pratensis L.） representing different types （＇Baron＇, BVMG type; ＇Blackstone＇, Cheri type; ＇Kenblue＇, Midwest type; and ＇Bluemoon＇, Midnight type） were chosen for this study. Grass materials were established in a glasshouse, and the changes in their photosynthetic and metabolic characteristics were analyzed during a period of imposed drought stress. Drought stress led to decrease in chlorophyll content, photosynthetic rate, stomatal conductance and transpiration rate in all four cultivars, but the relative changes in each characteristic varied among varieties. The results also showed that changes in the photosynthetic and metabolic characteristics of different varieties under drought stress were related to the morphological characteristics of the plants. Varieties with longer narrow leaves, longer roots, higher root-to-shoot ratios, and higher chlorophyll contents showed greater rates of photosynthesis and stomatal conductance, and lower transpiration rates.

盐胁迫条件下竹柳光合作用日变化特征研究

以竹柳盆栽苗木为试验材料,分析其在不同盐分浓度下的净光合速率、气孔导度、蒸腾速率、胞间CO_(2)浓度等光合生理指标的日变化特征。结果表明:竹柳在对照与轻度胁迫条件下,净光合速率的日变化特征表现为双峰型;在中度和重度胁迫条件下,为单峰型曲线,变化趋势表现为先上升后下降。气孔导度在对照与轻度胁迫条件下的日变化特征表现为双峰型;在中度和重度胁迫条件下为单峰型曲线,呈逐渐下降的趋势。蒸腾速率的日变化特征与气孔导度类似。各胁迫处理胞间CO_(2)浓度均较对照高,且随胁迫程度的加剧,胞间CO_(2)浓度也随之上升。

Transpiration of the Tamarind Artificial Forest in the Arid-Hot Valleys of Jinshajiang River,Yunnan

The transpiration characteristics of the tamarind artificial forest in the arid-hot valleys of Jinshajiang River, Yunnan were investigated through the measurement of the transpiration rate, stomatal conductance of tamarind leaf and the related envi- ronmental factors. The results showed that the transpiration of the tamarind in the clear sky had the close positive correlations with photosynthetically active radiation and air temperature and negative correlation with atmosphere moisture in the whole growth period. The daily change tendency of the transpiration rate was the similar with stomatal conductance, and the transpiration had the positive correlation with stomatal conductance. The transpiration rate of tamarind was highest in the rainy season of June and July and was relatively low in the drought season of March and April. The transpiration water consumption in rainy season of June and July was obviously higher than that in drought season of March and April. It fully suggested that the tamarind showed very good drought resistance and adaptability to the arid-hot valleys of Yunnan .

不同长势山桐子林光合指标日变化特点分析

以贵州省安顺市普定县山桐子林为研究地,分析不同长势山桐子林光合指标日变化特点。选择5年生山桐子林为研究对象,按照其不同长势,分为3组,分别为长势良好组、长势一般组和长势较差组,对比不同长势山桐子林光合指标变化特点。研究结果表明:各组山桐子林的蒸腾速率、净光合速率和气孔导度光合指标日变化特点基本一致,其中,长势良好组蒸腾速率、净光合速率和气孔导度均高于长势一般组和长势较差组(P<0.05)。由结果可知,不同长势山桐子林的光合指标日变化特点存在比较显著差异,长势约好,蒸腾速率、净光合速率和气孔导度等光合指标越高,说明山桐子林和光合指标之间呈正相关关系,山桐子林长势约好,光合作用越强,也进一步证实,光合作用对山桐子林的生长有较大影响。

Gas exchange and water relations of young potted loquat cv.Algerie under progressive drought conditions

Relationships between plant water status and gas exchange parameters at increasing levels of water stress were determined in Algerie loquats which grown in 50 I pots. Changes in soil water content and stem water potential and their effects on stomatal conductance （Gs ） and net photosynthesis （Pn） rate were followed in control plants and in plants without irrigation until the latter reached near permanent wilting point and some leaf abscission took place. Then, the irrigation was restarted and the comparison repeated. Soil water content and stem water potential gradually diminished in response to drought reaching the minimum values of 0.9 mm and -5.0 MPa, respectively, 9 days after watering suspension. Compromised plant water status had drastic effects on Gs values that dropped by 97％ in the last day of the drought period. Pn was diminished by 80％ at the end of the drought period. The increasing levels of water stress did not cause a steady increase in leaf temperature in non-irrigated plants. Non-irrigated plants wilted and lost some leaves due to the severity of the water stress. However, all non-irrigated plants survived and reached similar Pn than control plants just a week after the irrigation was restarted, confirming drought tolerance of loquat and suggesting that photosynthesis machinery remained intact.

Responses of Transgenic Tobacco Plants with Increased Proline Content to Drought and/or Heat Stress

Transgenic tobacco plants (M51-1) constitutively over-expressing a modified gene for the proline biosynthetic enzyme △2-pyrroline-5-carboxylate synthetase (P5CSF129A) and the corresponding wild-type plants (WT) were compared during drought or heat stress and under combination of both stresses. The proline content in M51-1 was several times higher than in WT plants. Under optimal conditions, the transpiration rate and stomatal conductance of M51-1 plants were lower than those in WT plants. The differences in net photosynthetic rate were not significant and water use efficiency and contents of chlorophyll and xanthophyll cycle pigments were higher in M51-1 than in WT plants. Drought induced by cessation of watering for 7 d resulted in decrease of all gas exchange parameters and chlorophyll content, but in an increase of the content of xanthophyll cycle pigments and degree of their de-epoxidation. After application of heat stress (40°C/60 min) to control or water-stressed plants the gas exchange parameters decreased considerably. Short-term heat stress alone, however, did not affect pigment contents. The responses of M51-1 and WT plants to the tested stresses did not differ significantly. Therefore, a decisive contribution of elevated proline content to drought or heat stress tolerance of tobacco was not proved.

Effects of Multi-Stage Continuous Drought on Photosynthetic Characteristics, Yield and Water Use Efficiency of Winter Wheat

A drought event can cause entire crops to fail or yield loss.In order to study the effects of continuous drought on photosynthetic characteristics,yield,and water use efficiency(WUE)of winter wheat(Triticum aestivum L.),the winter wheat variety“Aikang 58”was selected as test material with controlling the water of the pot-planted winter wheat under a mobile rainout shelter.Based on foot planting and safe wintering,winter wheat was evaluated under different drought conditions,including light,moderate and severe drought at the jointing(B),heading(C),and filling(G)stages.The soil water content was controlled in a range of 60%to 70%,50%to 60%,and 40%to 50%of the field capacity,respectively.In the experiment,there were 9 single-stage droughts,3 three-stage droughts,and 1 test control(totaling 13 trials).The results are as follows:Under a single-stage drought,the change of net photosynthetic rate(Pn)and stomatal conductance(Gs)have similar trends,and they both decrease significantly with the severity of the drought.Under three-stage continuous droughts,the change curve of Gs shows a constant downward trend;the change curve of Pn showed a“valley shape,”and the minimum value of Pn appeared at the heading stage.All droughts will reduce the yield of winter wheat.Under the three-stage continuous drought conditions,except for light drought,moderate drought and severe drought will cause significant yield reduction,mainly due to lack of water at the jointing and heading stages.Continuous drought will reduce the WUE,and the difference will reach a significant level under moderate and severe drought.The present results suggested that when water resources are scarce,it is a better irrigation model to save water and achieve high grain yield by applying appropriate water stress(60%–70%FC)during the critical growth period of winter wheat.