Targeted mutagenesis of amino acid transporter genes for rice quality improvement using the CRISPR/Cas9 system

High grain protein content(GPC) reduces rice eating and cooking quality(ECQ). We generated OsAAP6 and OsAAP10 knockout mutants in three high-yielding japonica varieties and one japonica line using the CRISPR/Cas9 system. Mutation efficiency varied with genetic background in the T_0 generation, and GPC in the T_1 generation decreased significantly,owing mainly to a reduction in glutelin content. Amylose content was down-regulated significantly in some Osaap6 and all Osaap10 mutants. The increased taste value of these mutants was supported by Rapid Visco Analysis(RVA) profiles, which showed higher peak viscosity and breakdown viscosity and lower setback viscosity than the wild type. There were no significant deficiencies in agronomic traits of the mutants. Targeted mutagenesis of OsAAP6 and OsAAP10, especially OsAAP10, using the CRISPR/Cas9 system can rapidly reduce GPC and improve ECQ of rice, providing a new strategy for the breeding cultivars with desired ECQ.

Improving the efficiency of transport systems using simulation

The article describes the possibilities of application of simulation modeling for the analysis of infrastructure and technology of transport services of enterprises. The main technological and possible economic effects for the enterprises arising at performance of modeling of a transport component of their work are resulted.

The effects of transportation system improvements on urban performances with heterogeneous residents

Through developing a spatial equilibrium model for a linear monocentric city with a bimodal traffic corridor(i.e.,highway and public transit),this paper examines the effects of transportation improvements(including the decreases of the fixed travel cost,the travel time and monetary cost per unit distance)on urban spatial structure and the utilities of the two resident classes,namely car-available-residents(CARs)and car-unavailable-residents(CURs).As a result,it is demonstrated that the city size increases with the improvements of vehicle-highway system and the degree of CARs’travel mode choice rationality,but the improvement of public transit may produce a shrinkage rather than an expansion in city size.From the aspect of utility,both CARs and CURs benefit from the improvement of vehicle-highway system;however,the improvement of public transit might have an adverse effect on the utility of CARs.These findings would provide valuable reference for the future transportation and urban planning.

Strategies for Improving Public Transportation Accessibility in Beijing's Urban Regeneration from an Inclusive Perspective: Inspirations from Paris's Experience

As China’s urbanization has transformed from extensive development to "refined operation," urban regeneration is more significant. This paper analyzes changes in research perspectives of urban regeneration in recent years at home and abroad and the innovative concept of inclusive development proposed in the context of globalization, finding that the connotation of urban regeneration in European countries has been gradually enriched in order to promote social integration. Based on this, the paper examines how Paris, the first European city, combined inclusive development and urban regeneration and addressed social integration and equity in urban regeneration by taking accessibility as a key point in solving the most fundamental transportation problems. Based on lessons learned from Paris in developing a barrier-free transportation system, it proposes specific optimization strategies at the micro level in accordance with the status quo of public transportation accessibility in Beijing’s urban regeneration.

Co-doping method used to improve the charge transport balance in solution processed OLEDs

In this paper, co-doping method is used to improve the current efficiency of solution-processed organic light-emitting diodes(OLEDs). By changing the ratio of two thermally activated delayed fluorescent(TADF) emitters, we studied the performance of device and its mechanism. A solution processed OLED with a structure of indium tin oxide(ITO, 150 nm)/PEDOT:PSS(30 nm)/CBP:4 Cz IPN-x%:4 Cz PN-y%(30 nm)/TPBi(40 nm)/Li F(1 nm)/Al(100 nm) was fabricated. The current efficiencies of 26.6 cd/A and 26.4 cd/A were achieved by the devices with dopant ratio of 6% 4 Cz IPN:2% 4 Cz PN and 2% 4 Cz IPN:6% 4 Cz PN in emitting material layer(EML), respectively. By investigating the tendency of current density change in devices with different doping ratio, we suggested that the enhancement of the current efficiency should be due to the charge transport balance improvement induced by assist dopant in EML.