Abstract： Olive oil adulteration with less expensive edible oils is a major issue for the olive oil industry. Eleven vegetable oils were selected and purchased from different markets in Iraqi Kurdistan region （Erbil...Abstract： Olive oil adulteration with less expensive edible oils is a major issue for the olive oil industry. Eleven vegetable oils were selected and purchased from different markets in Iraqi Kurdistan region （Erbil City center）. In this study high performance liquid chromatography （HPLC） on reversed-phase （RP） column with refractive index （RI） detector was used to classify some components in different edible oils depending on calculation of partition number. Adulteration of one of the olive oil samples （01） was identified, due to the appearance of glycerol trilinoleate （LLL） peak in the HPLC chromatogram, while this peak is not present in HPLC chromatograms of other olive oil samples. To ensure the presence of LLL a standard concentration of LLL was added to the sample and increasing the peak height at the retention time of LLL indicates the presence of LLL and the adulteration of olive oil sample.展开更多
Effects of different iron concentrations(final concentrations of iron in Artari’s medium:3.7,37.0,74.0,and 185.0 mmol$L-1)on growth rate and contents of protein,triacylglycerides,andβ-carotene in Dunaliella viridis ...Effects of different iron concentrations(final concentrations of iron in Artari’s medium:3.7,37.0,74.0,and 185.0 mmol$L-1)on growth rate and contents of protein,triacylglycerides,andβ-carotene in Dunaliella viridis cells at cultivation in different months were investigated.It was shown that the dose-dependent effects of iron were notable for season variability.In the 1st experimental series(October,2007),iron at researched concentrations did not affect growth rate of culture and protein,triacylglyceride,andβ-carotene contents in cells.In experimental series conducted respectively in November 2007,December 2007,and February 2008,the dosedependent stimulation of microalgae growth was observed.For each of these experimental series,there were particular dose dependences of protein,triacylglyceride,andβ-carotene contents in microalgae cells at cultivation on media with iron at different concentrations.Meanwhile,for all of the four experimental series conducted in different months,variability of growth rate and analyzed parameters of microalgae Dunaliella viridis as control(cultivation without iron)was shown.It is suggested that these functional differences of control cultures of microalgae in different months caused variability in the dosedependent effects of iron in a Dunaliella viridis culture.The possibility of iron usage for increasing microalgae biomass and for enriching it byβ-carotene in Dunaliella viridis culture with initial low productivity and lowβ-carotene content is considered.展开更多
The SH-SY5Y,neuroblastoma cell line,is a common in vitro model used to study physiological neuronal function and the neuronal response to different stimuli,including exposure to toxic chemicals.These cells can be diff...The SH-SY5Y,neuroblastoma cell line,is a common in vitro model used to study physiological neuronal function and the neuronal response to different stimuli,including exposure to toxic chemicals.These cells can be differentiated to neuron-like cells by administration of various reagents,including retinoic acid or phorbol12-myristate-13-acetate.Despite their common use,there is an incomplete understanding of the molecular changes that occur during differentiation.Therefore,there is a critical need to fully understand the molecular changes that occur during differentiation to properly study neurotoxicity in response to various environmental exposures.Previous studies have investigated the proteome and transcriptome during differentiation;however,the regulation of the cellular lipidome in this process is unexplored.In this work,we conducted liquid chromatography−mass spectrometry(LC−MS)-based untargeted lipidomics in undifferentiated and differentiated SH-SY5Y cells,induced by retinoic acid.We show that there are global differences between the cellular lipidomes of undifferentiated and differentiated cells.Out of thousands of features detected in positive and negative electrospray ionization modes,44 species were identified that showed significant differences(p-value≤0.05,fold change≥2)in differentiated cells.Identification of these features combined with targeted lipidomics highlighted the accumulation of phospholipids,sterols,and sphingolipids during differentiation while triacylglycerols were depleted.These results provide important insights into lipid-related changes that occur during cellular differentiation of SH-5YSY cells and emphasize the need for the detailed characterization of biochemical differences that occur during differentiation while using this in vitro model for assessing ecological impacts of environmental pollutants.展开更多
文摘Abstract： Olive oil adulteration with less expensive edible oils is a major issue for the olive oil industry. Eleven vegetable oils were selected and purchased from different markets in Iraqi Kurdistan region （Erbil City center）. In this study high performance liquid chromatography （HPLC） on reversed-phase （RP） column with refractive index （RI） detector was used to classify some components in different edible oils depending on calculation of partition number. Adulteration of one of the olive oil samples （01） was identified, due to the appearance of glycerol trilinoleate （LLL） peak in the HPLC chromatogram, while this peak is not present in HPLC chromatograms of other olive oil samples. To ensure the presence of LLL a standard concentration of LLL was added to the sample and increasing the peak height at the retention time of LLL indicates the presence of LLL and the adulteration of olive oil sample.
文摘Effects of different iron concentrations(final concentrations of iron in Artari’s medium:3.7,37.0,74.0,and 185.0 mmol$L-1)on growth rate and contents of protein,triacylglycerides,andβ-carotene in Dunaliella viridis cells at cultivation in different months were investigated.It was shown that the dose-dependent effects of iron were notable for season variability.In the 1st experimental series(October,2007),iron at researched concentrations did not affect growth rate of culture and protein,triacylglyceride,andβ-carotene contents in cells.In experimental series conducted respectively in November 2007,December 2007,and February 2008,the dosedependent stimulation of microalgae growth was observed.For each of these experimental series,there were particular dose dependences of protein,triacylglyceride,andβ-carotene contents in microalgae cells at cultivation on media with iron at different concentrations.Meanwhile,for all of the four experimental series conducted in different months,variability of growth rate and analyzed parameters of microalgae Dunaliella viridis as control(cultivation without iron)was shown.It is suggested that these functional differences of control cultures of microalgae in different months caused variability in the dosedependent effects of iron in a Dunaliella viridis culture.The possibility of iron usage for increasing microalgae biomass and for enriching it byβ-carotene in Dunaliella viridis culture with initial low productivity and lowβ-carotene content is considered.
基金the support from the National Science Foundation(CBET-2112201)。
文摘The SH-SY5Y,neuroblastoma cell line,is a common in vitro model used to study physiological neuronal function and the neuronal response to different stimuli,including exposure to toxic chemicals.These cells can be differentiated to neuron-like cells by administration of various reagents,including retinoic acid or phorbol12-myristate-13-acetate.Despite their common use,there is an incomplete understanding of the molecular changes that occur during differentiation.Therefore,there is a critical need to fully understand the molecular changes that occur during differentiation to properly study neurotoxicity in response to various environmental exposures.Previous studies have investigated the proteome and transcriptome during differentiation;however,the regulation of the cellular lipidome in this process is unexplored.In this work,we conducted liquid chromatography−mass spectrometry(LC−MS)-based untargeted lipidomics in undifferentiated and differentiated SH-SY5Y cells,induced by retinoic acid.We show that there are global differences between the cellular lipidomes of undifferentiated and differentiated cells.Out of thousands of features detected in positive and negative electrospray ionization modes,44 species were identified that showed significant differences(p-value≤0.05,fold change≥2)in differentiated cells.Identification of these features combined with targeted lipidomics highlighted the accumulation of phospholipids,sterols,and sphingolipids during differentiation while triacylglycerols were depleted.These results provide important insights into lipid-related changes that occur during cellular differentiation of SH-5YSY cells and emphasize the need for the detailed characterization of biochemical differences that occur during differentiation while using this in vitro model for assessing ecological impacts of environmental pollutants.
