Rats of both sexes were fed on a basal feed containing 1% refined konjac meal (RKM) for 18 months and the effects of RKM on the cell aging were observed. A comparable group fed on the basic feed was used as the contro...Rats of both sexes were fed on a basal feed containing 1% refined konjac meal (RKM) for 18 months and the effects of RKM on the cell aging were observed. A comparable group fed on the basic feed was used as the control. Results obtained demonstrate that the long-term feeding of RKM to rats can delay the course of cell aging of the gliocyte, cadiomyocyte, and the endothelial cell of the large and medium arteriases, hence it is likely to delay the occurrence of arteriosclerosis and improve the functions of the brain, heart and vascular system.展开更多
Effects of refined konjac meal (RKM) on the calcium and phosphorus metabolism and bone parameters were observed in rats of both sexes fed with food containing 1% of RKM for 18 months. A comparable group of rats fed on...Effects of refined konjac meal (RKM) on the calcium and phosphorus metabolism and bone parameters were observed in rats of both sexes fed with food containing 1% of RKM for 18 months. A comparable group of rats fed on basic diet only was used as a control.Results obtained indicate that all the measured parameters (serum calcium and phosphorus level, femur weight and its calcium and phesphorus content, and the osteometry of the tibia) showed no significant difference between the experimental and the control groups.Thus there is no adverse effect either on the calcium and phosphorus metabolism or on the bone after a long-term intake at a moderate dosage.展开更多
Numerous studies currently compare the lipid metabolism in patients with cardiovascular disease(CVD)and healthy individuals to identify lipid markers for predicting CVD.In this study,multidimensional mass spectrometry...Numerous studies currently compare the lipid metabolism in patients with cardiovascular disease(CVD)and healthy individuals to identify lipid markers for predicting CVD.In this study,multidimensional mass spectrometry-based shotgun lipidomics was used to examine the serum lipidomics of participants in a clinical randomized controlled feeding trial undergoing olive oil(OO),camellia seed oil(CSO),and soybean oil(SO)dietary interventions.189 lipid molecules are identified,including 14 species of phosphatidylinositol,45 species of ethanolamine glycerols(PE),47 species of choline glycerophospholipids(PC),39 species of triacylglycerols(TAG),18 species of lysophosphatidylcholine,and 26 species of sphingomyelin.After screening,10 lipid markers are found,among which 18:2 fatty acid(FA),16:1 FA,C54:4/C55:11,C54:3/C55:10,and C52:3/C53:10 in TAG pool,p18:0/20:0 and a18:0/18:1 in PC pool,and p18:1/20:4 in PE pool have differential regulation in the SO group compared to OO and CSO.The d16:0/18:1 in PC pool and C52:2/C53:9 in TAG pool are differentially regulated by OO and CSO.The C52:2/C53:9 in TAG pool has a significant negative correlation with aspartate aminotransferase(r=-0.363,P=0.048)and high-density lipoprotein cholesterol(r=-0.519,P<0.01).This study provides a reference for researching the effect of dietary fat on blood lipid metabolism.展开更多
Fragments of DNA present in food and feed are taken up by the gastrointestinal tract (GIT) of mammals. The extent of uptake varies according to organism, study design and DNA source. This study explores the hypothesis...Fragments of DNA present in food and feed are taken up by the gastrointestinal tract (GIT) of mammals. The extent of uptake varies according to organism, study design and DNA source. This study explores the hypothesis that actively growing, as well as pregnant rats, are more likely to take up DNA from the GIT than mature animals due to the high demand for nutrients for tissue and organ development. Plasmid DNA (pDNA) was added to standard feed for growing, and pregnant rats. The young rats received one pDNA (50 μg) containing meal by gavage. Blood, organ and tissue samples were harvested at 2 h to 3 days post feeding (p.f). The pregnant females were fed pellets containing pDNA (100 μg) daily, starting at day 5 after established pregnancy. Females and foeti were killed at days 7 and 14 of gestation, and pups at the time of weaning. Genomic DNA was analyzed by PCR followed by Southern blot and real-time PCR. A 201 bp target sequence was detected in mesenteric lymph nodes, spleen, liver and pancreas from growing rats 2 h p.f. At 6 h, target DNA was detectable in the kidneys, and at three days p.f. in the liver. Target DNA was not detected in samples from pregnant rats, their foeti or pups. In conclusion, low level of feed introduced DNA could be transiently detected in organs of young, growing rats. However, indications of increased DNA uptake levels in the GIT of growing rats were not found.展开更多
文摘Rats of both sexes were fed on a basal feed containing 1% refined konjac meal (RKM) for 18 months and the effects of RKM on the cell aging were observed. A comparable group fed on the basic feed was used as the control. Results obtained demonstrate that the long-term feeding of RKM to rats can delay the course of cell aging of the gliocyte, cadiomyocyte, and the endothelial cell of the large and medium arteriases, hence it is likely to delay the occurrence of arteriosclerosis and improve the functions of the brain, heart and vascular system.
文摘Effects of refined konjac meal (RKM) on the calcium and phosphorus metabolism and bone parameters were observed in rats of both sexes fed with food containing 1% of RKM for 18 months. A comparable group of rats fed on basic diet only was used as a control.Results obtained indicate that all the measured parameters (serum calcium and phosphorus level, femur weight and its calcium and phesphorus content, and the osteometry of the tibia) showed no significant difference between the experimental and the control groups.Thus there is no adverse effect either on the calcium and phosphorus metabolism or on the bone after a long-term intake at a moderate dosage.
基金funded by the“Pioneer”and“Leading Goose”R&D Program of Zhejiang Province(2023C02044)the Key Research and Development Program for Science and Technology Projects of Zhejiang Province(2017C02003)The funding sources played no role in the study design,study implementation,data analysis,or manuscript preparation.Science and Technology Projects of Zhejiang Province(2017C02003).
文摘Numerous studies currently compare the lipid metabolism in patients with cardiovascular disease(CVD)and healthy individuals to identify lipid markers for predicting CVD.In this study,multidimensional mass spectrometry-based shotgun lipidomics was used to examine the serum lipidomics of participants in a clinical randomized controlled feeding trial undergoing olive oil(OO),camellia seed oil(CSO),and soybean oil(SO)dietary interventions.189 lipid molecules are identified,including 14 species of phosphatidylinositol,45 species of ethanolamine glycerols(PE),47 species of choline glycerophospholipids(PC),39 species of triacylglycerols(TAG),18 species of lysophosphatidylcholine,and 26 species of sphingomyelin.After screening,10 lipid markers are found,among which 18:2 fatty acid(FA),16:1 FA,C54:4/C55:11,C54:3/C55:10,and C52:3/C53:10 in TAG pool,p18:0/20:0 and a18:0/18:1 in PC pool,and p18:1/20:4 in PE pool have differential regulation in the SO group compared to OO and CSO.The d16:0/18:1 in PC pool and C52:2/C53:9 in TAG pool are differentially regulated by OO and CSO.The C52:2/C53:9 in TAG pool has a significant negative correlation with aspartate aminotransferase(r=-0.363,P=0.048)and high-density lipoprotein cholesterol(r=-0.519,P<0.01).This study provides a reference for researching the effect of dietary fat on blood lipid metabolism.
基金The work was funded by the Research Council of Norway(148704).
文摘Fragments of DNA present in food and feed are taken up by the gastrointestinal tract (GIT) of mammals. The extent of uptake varies according to organism, study design and DNA source. This study explores the hypothesis that actively growing, as well as pregnant rats, are more likely to take up DNA from the GIT than mature animals due to the high demand for nutrients for tissue and organ development. Plasmid DNA (pDNA) was added to standard feed for growing, and pregnant rats. The young rats received one pDNA (50 μg) containing meal by gavage. Blood, organ and tissue samples were harvested at 2 h to 3 days post feeding (p.f). The pregnant females were fed pellets containing pDNA (100 μg) daily, starting at day 5 after established pregnancy. Females and foeti were killed at days 7 and 14 of gestation, and pups at the time of weaning. Genomic DNA was analyzed by PCR followed by Southern blot and real-time PCR. A 201 bp target sequence was detected in mesenteric lymph nodes, spleen, liver and pancreas from growing rats 2 h p.f. At 6 h, target DNA was detectable in the kidneys, and at three days p.f. in the liver. Target DNA was not detected in samples from pregnant rats, their foeti or pups. In conclusion, low level of feed introduced DNA could be transiently detected in organs of young, growing rats. However, indications of increased DNA uptake levels in the GIT of growing rats were not found.
