Antioxidant Activity and Soothing Efficacy of Tambourissa Trichophylla Leaf Extract in Vitro Study

Present study is aimed to investigate in vitro biological efficacy of Tambourissa trichophylla leaf extract(TTLE),a plant-derived ingredient in cosmetic,on antioxidant activity and soothing efficacy.The total phenols content of TTLE was determined by the Folin-Ciocalteu method,antioxidant activity was tested by DPPH free radical scavenging assay,hyaluronidase inhibition activity was performed by using in vitro enzyme inhibitory assays based on spectrophotometric evaluation,and the soothing efficacy of TTLE was performed by the expression of nitric oxide(NO)and interleukin-6(IL-6)produced by LPS-induced RAW264.7 cells.The results shown that the total phenolic content of TTLE was as high as 54%,the DPPH scavenging activity was 81.18%at the concentration of 25μg/mL,the hyaluronidase inhibition activity was 69.04%at the concentration of 5 mg/mL,and the inhibition of NO and IL-6 were 15.70%and 76.57%at 0.05 mg/mL,respectively.TTLE has a high content of total phenols,high antioxidant activity and soothing effects,which indicated that TTLE was a promising ingredient in the development and application of cosmetics.

一种毛叶杯轴花叶提取物的体外抗氧化和舒缓功效研究

探究了一种毛叶杯轴花(Tambourissa trichophylla)叶提取物的活性成分、抗氧化能力和舒缓功效。通过福林酚法对其总酚含量进行测定,并通过DPPH自由基清除实验对其抗氧化能力进行测试,采用透明质酸酶活性抑制实验及通过脂多糖(LPS)诱导小鼠单核巨噬细胞(RAW264.7)产生的一氧化氮(NO)、白细胞介素6(IL-6)的表达量来评价其舒缓功效。结果显示,毛叶杯轴花叶提取物总酚含量高达54%;在浓度为25μg/mL时,DPPH自由基清除率达到81.18%;在浓度为5 mg/mL时,透明质酸酶抑制率达到69.04%;在浓度为0.05 mg/mL时,对LPS诱导RAW246.7细胞产生的NO和IL-6抑制率分别为15.70%、76.57%。以上说明,毛叶杯轴花叶提取物活性成分含量高,具有较强的抗氧化和舒缓功效,在化妆品的开发与应用中具有重要价值。