苋菜红素的提取纯化及抗氧化活性分析

通过单因素和正交试验优化苋菜(Amaranthus tricolor L.)红素的提取工艺。结果表明,提取苋菜红素的最佳条件为浸提时间30 min,浸提温度40℃,料液比1∶40(g/m L),同时利用大孔树脂进行纯化,并测定苋菜红素的含量及抗氧化活性,得到苋菜红素的含量为12.5%,在一定质量浓度范围内,苋菜红素还原能力随浓度升高而增强,苋菜红素对羟自由基、亚硝酸根离子和DPPH·的最大清除率分别为54.2%、17.3%和97.2%,具有明显的抗氧化作用。

过氧化钙对苋菜常温活体保鲜效果的影响

向营养液中添加过氧化钙,考察其对苋菜(Amaranthus tricolor L.)常温活体保鲜效果的影响。结果表明,与不添加过氧化钙的处理相比,0.025、0.100、0.250 g/L过氧化钙处理均能极显著延长苋菜的货架期,并延缓苋菜含水量、叶绿素、可溶性糖和维生素C含量的降低;其中以0.100 g/L过氧化钙的保鲜效果最佳。

三色堇叶片DNA不同提取方法比较

以三色堇(Viola tricolor L.)叶片为材料,采用SDS法、CTAB法及改良CTAB法分别对三色堇叶片DNA进行了提取,并用琼脂糖凝胶电泳和紫外光谱对DNA提取质量和浓度进行检测。结果表明,改良CTAB法提取的DNA纯度最高,提取效率较高;SDS法提取的DNA含有较多的蛋白质或糖类杂质;CTAB法虽然能够除去蛋白质等杂质,但是DNA浓度低于改良CTAB法。

Luminescence and Preparation of LED Phosphor Ca_8Mg(SiO_4)_4Cl_2∶Eu^(2+)

Calcium magnesium chlorosilicate doped by europium, Ca8Mg（SiO4）4Cl2： Eu^2＋, was prepared by the solid state reaction at high temperature. The compound obtained is pure Ca8Mg（SiO4）4Cl2 phase with cubic structure. Its average particle size is 5 μm, and it has good dispersity and morphological form. The excitation spectrum of Ca8Mg（SiO4）4Cl2： Eu^2＋ is a wide band, which covers from 270 to 480 nm. The emission spectrum is also a wide band peaked at 510 nm. The luminescent intensity reaches to the maximum when the concentration of Eu^2 ＋ is 2%. The wavelength of emission and excitation of the phosphor with various Eu^2 ＋ contents keeps constant. This spectrum range matches violet and blue LED chips very well, and its strong luminescence intensity is suitable for a green phosphor of tricolor phosphor of white light LED.

Effects of Copper on the Photosynthesis and Oxidative Metabolism of Amaranthus tricolor Seedlings

The objective of the present study was to gain better insight into the physiological mechanisms on the effects of copper （Cu） on photosynthesis and active oxygen metabolism in three-colored amaranth plant （Amaranthus tricolor）. Three- colored amaranth seedlings were subjected to different Cu levels in soils during the entire experimental period. The parameters of growth, photosynthesis, mineral elements contents, and active oxygen metabolism were investigated using plant physiological methods. The results showed that 2.0 and 4.0 mmol Cu kg^-1 treatments decreased the whole plant biomass to 91 and 73% of the control, respectively. The net photosynthetic rate （Pn） and the stomatal conductance （gs） were similarly reduced in the third leaves of three-colored amaranth seedlings treated with 2.0 and 4.0 mmol Cu kg^-1 soil, respectively. None of the investigated Cu levels decreased the internal CO2 concentration （Ci）. The effect of Cu on the potential efficiency of photosystem Ⅱ （Fv/Fm） was negligible, whereas the effect of Cu on the PS Ⅱ quantum efficiency （ΦPS Ⅱ） after plant adaptation in actinic irradiation was more noticeable. On the other hand, decreases in water percentage, contents of photosynthetic pigments and mineral elements including Fe, K, and Mg, and significant increase in the Cu content were observed in the third leaves of Cu-treated plants. Superoxide dismutase （SOD） and peroxidase （POD） activities as well as the proline （Pro） content significantly increased in the third leaves of the three-colored amaranth seedlings treated with 2.0 and 4.0 mmol Cu kg^-1 soil, while catalase （CAT） and ascorbate peroxidase （APX） activities as well as the contents of carotenoid （Car）, glutathione （GSH）, and ascorbic acid （AsA） decreased, and accompanied by the increases in the contents of hydrogen peroxide （H2O2）, superoxide anion （O2^-）, and malondialdehyde （MDA）, and electrolyte leakage. As a result of the imbalance of active oxygen metabolism, Pn and ΦPS Ⅱ decreased, and peroxidization enhanced under levels of 2.0 and 4.0 mmol Cu kg^-1 soil. Finally, the growth of three-colored amaranth plant was significantly inhibited.

菌株Marasmius tricolor 310b发酵粗酶液对3种染料脱色效果的研究

为探索分离自青藏高原高寒草地产漆酶新真菌菌株Marasmius tricolor 310b对苯甲烷类、蒽醌类和偶氮类染料的脱色效果,采用摇瓶发酵法获得漆酶粗酶液进行脱色实验。结果表明菌株Marasmius tricolor 310b漆酶发酵粗酶液都能够有效的降解溴甲酚绿(苯甲烷类)、茜素红(蒽醌类)、刚果红(偶氮类) 3种染料,但降解效果有所差异,其中,以苯甲烷类染料脱色作用最优。另发现菌株Marasmius tricolor 310b发酵粗酶液对3种染料的降解程度与波长和反应时间有直接关系,随着反应时间的延长,降解效率有不同程度提高。我们的研究初步揭示了新菌株Marasmius tricolor 310b发酵粗酶液对苯甲烷类、蒽醌类、偶氮类具有明显的脱色效果,这一发现对于更新具有脱色效果的真菌资源库有一定的理论支持。

使用金刚石绳锯切割打捞沉船

采用金刚石绳锯切割打捞Tricolor号沉船是技术上的一大进步，在此之前，没有一家公司打捞过这样大型的船舶，更没有打捞过一艘象这样的装有3000台各式轿车(奥迪、宝马、沃尔沃和绅宝)、起重机、推土机的货船。海洋打捞公司使用金刚石绳锯在水下将这艘货船锯成碎片，然后把废料运到泽布吕赫(ZeebrUEgge)港进行处理。

Sesquiterpenoids and an ergosterol from cultures of the fungus Daedaleopsis tricolor

Four new bisabolane sesquiterpenoids daedatrins A-D(1-4),a cadinane sesquiterpene 12-hydroxy-α-cadinol(5),and a heptanorergosterane derivative daedatrin G(6)were isolated from cultures of the basidiomycete Daedaleopsis tricolor.Their structures were elucidated by spectroscopic methods including extensive 2D NMR techniques and X-ray crystallography.All the compounds showed no significant activity against five human cancer cell lines.

Effect of H_2O_2 on Growth of Collard( Brassis oleracea L. ) Seedlings Under Salt Stress

Collard variety( Brassis oleracea L. var. acephala f. tricolor Hort.) as a research material was treated with exogenous H_2O_2 and H_2O_2 scavenger dimethyl thiourea under 100 mmol/L NaC l stress. Two days later,growth rate,dry weight,fresh weight and relative water content of the plants were determined. After 6h of treatment,the activity and gene expression of three antioxidant enzymes,superoxide dismutase( SOD),catalase( CAT) and ascorbate peroxidase( APX) in plants,were measured. The results showed that the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in collard seedlings were higher in the treatment of salt stress with the addition of 0. 05 mmol/L exogenous H_2O_2 than in the simple salt stress treatment; and when endogenous H_2O_2 was removed,the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in plant seedlings were lower than those under simple salt stress. It is speculated that under salt stress,H_2O_2 is involved in the regulation of antioxidant defense gene expression,and it might be an important regulator of salt-induced antioxidant system in collard leaves.

20.231 Gbit/s tricolor red/green/blue laser diode based bidirectional signal remodulation visible-light communication system

We propose and experimentally demonstrate a recorded 1-m bidirectional 20.231-Gbit/s tricolor R/G/B laser diode(LD) based visible-light communication(VLC) system supporting signal remodulation. In the signal remodulation system, an LD source is not needed at the client side. The client reuses the downstream signal sent from the central office(CO) and remodulates it to produce the upstream signal. As the LD sources are located at the CO, the laser wavelength and temperature managements at the cost-sensitive client side are not needed.This is the first demonstration, to our knowledge, of a >20 Gbit∕s data rate tricolor R/G/B VLC signal transmission supporting upstream remodulation.

Tricolor emitting and energy transfer in the phosphor Ba_2ZnSi_2O_7:Ce^(3+),Eu^(3+),Eu^(2+) for white-LED based near-UV chips

The red, green and blue （R/G/B） tricolor emitting phosphors Ba2ZnSi2O7 co-doped with Ce3＋and Eu3＋were synthesized in air atmosphere by a conventional high temperature solid-state reaction technique. All of the excitation spectrum of the phosphor Ba2ZnSi2O7：Ce,Eu showed a strong broad band absorption in the n-UV region whenever monitored by red （630 nm）-emitting or by green （500 nm）-and blue （402 nm）-emitting. Under the excitation of 330 nm, the emission spectrum containing a blue-violet emis-sion band, a green emission and four sharp lines originated from the characteristic transitions of Ce3＋, Eu2＋and Eu3＋ions, of which the relative intensities of the three emission bands could be controlled by the doping concentration of Ce3＋. The ca. CIE chromaticity co-ordinates （x=0.317, y=0.309） of the phosphor Ba1.94ZnSi2O7：0.03Eu,0.01Ce was very close to the standard white （x=0.33, y=0.33）, which suggested that it was a novel single-phased white-light emitting phosphor for LED-based near-UV chip. The mechanisms of energy transfer from Eu2＋to Eu3＋via Ce3＋was also discussed.

Color image single-channel encryption based on tricolor grating theory

A method of color image single-channel encryption is proposed. The proposed method uses tricolor grating to encode a color image into a gray level image, then the gray level image is encrypted by double random phase encryption, so a color image is encrypted in a single-channel and its security is ensured. Computer simulations and the chromatic aberration analysis are given to prove the possibility of the proposed idea.The optical system is simpler and is easy to be applied into practice. The simulation results show that this method is efficiency to encrypt a color image, and it is robust.

1株筛选自高寒草地土壤产漆酶真菌对2种染料脱色效果的研究

为了探索产漆酶真菌对染料的脱色效果,试验选用了1株筛选自高寒草地土壤的产漆酶真菌Marasmius tricolor 310b菌株,研究了溴甲酚绿和刚果红2种染料的脱色效果,通过制备发酵粗酶液,并在不同温度、pH值及金属离子条件下进行2种染料的脱色实验。结果表明:反应体系的起始pH值、温度、不同金属离子等因素对脱色效果的影响显著,主要表现为:pH=3.0时,粗酶液对溴甲酚绿的脱色率达75%;pH=4.0时,粗酶液对刚果红的脱色率达30%;温度在4℃~45℃范围内,脱色率随着温度升高而加强;粗酶液在不同温度下放置48,h后,脱色率保持不变;Ca^(2+)、Cu^(2+)、K^(+)、Na^(+)、Mg^(2+)、Mn^(2+)等金属离子对脱色率没有影响,Fe^(3+)和Fe^(2+)具有抑制作用。

Integration and Expression of Human Growth Hormone Gene in Caladium bicolor

Human growth hormone (hGH) gene has been inserted into the plasmid pLGV1103 to give the recombinant plasmid pLB-9. It has been introduced into the agrobacterium containing plasmid pGV3850. The recombinant Ti plasmid pGL198(hGH) has been obtained by homologous recombination. The monocotyledon Caladium bicolor has been transferred with pGL198 (hGH) with the leaf-disk co-cultivation method, and transgenic plants have been regenerated. The results of nopaline analysis, NPT II detection Southern blot and Western blot show that the hGH gene was integrated into the genome of Caladium bicolor, and a 22-kD protein was synthesized in the transgenic plants.

Tricolor microcavity OLEDs based on P-nc-Si:H films as the complex anodes

A P^＋-nc-Si：H film（boron-doped nc-Si：H thin film） was used as a complex anode of an OLED.As an ideal candidate for the composite anode,the P^＋-nc-Si：H thin film has a good conductivity with a high work function（- 5.7 eV） and outstanding optical properties of high reflectivity,transmission,and a very low absorption.As a result,the combination of the relatively high reflectivity of a P^＋-nc-Si：H film/ITO complex anode with the very high reflectivity of an Al cathode could form a micro-cavity structure with a certain Q to improve the efficiency of the OLED fabricated on it.An RGB pixel generated by microcavity OLEDs is beneficial for both the reduction of the light loss and the improvement of the color purity and the efficiency.The small molecule Alq would be useful for the emitting light layer（EML） of the MOLED,and the P^＋-nc-Si film would be used as a complex anode of the MOLED,whose configuration can be constructed as Glass/LTO/P^＋-nc-Si：H/ITO/MoO3/NPB/Alq/LiF/Al.By adjusting the thickness of the organic layer NPB/Alq,the optical length of the microcavity and the REB colors of the device can be obtained.The peak wavelengths of an OLED are located at 486,550,and 608 nm,respectively.The CIE coordinates are（0.21,0.45）,（0.33,0.63）,and（0.54,0.54）,and the full widths at half maximum（FWHM） are 35,32,and 39 nm for red,green,and blue,respectively.

MAGNETIC AND MOSSBAUER STUDY OF ANCIENT YAOZHOU TANG TRICOLOR CERAMICS

Ⅰ. INTRODUCTIONThe valuable information about the ancient ceramics can be acquired from studying their structures, compositions and manufacture technology by modern scientific and technical method. It is an important field of the modern scientific and technological archaeology (STA), which is also called experimental archaeology and has a special significance for the "country of ceramics" .