In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wilfordii Hook f.. Juvenile shoots from one-year-old elite plants were used as the source of explan...In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wilfordii Hook f.. Juvenile shoots from one-year-old elite plants were used as the source of explants. New axillary shoots were obtained after 30 days of culture on a MS medium supplemented with BAP （2.0 mg.L^-1） and NAA （0.1 mg.L^-1）. The optimal multiplication medium was a modified MS medium supplemented with BAP （1.0 mg.L^-1） and NAA （0.1 mg.L^-1）. This yielded a multiplication rate of 2.4 for each subculture. Slightly more than 92% of shoots rooted when cultured on a modified MS medium containing IBA （0.2 mg.L^-1） and activated charcoal （0.5 mg.L^-1）. Activated charcoal promoted both a strong and a high rooting rate during the rooting phase. Plantlets were transferred to pots for a short acclimatization stage in a greenhouse where 95% of the plantlets survived. This highly reproducible procedure can be adopted for large-scale propagation of T. wilfordii.展开更多
雷公藤（Tripterygium wilfordii）不仅是一种药用植物，还是一种有毒植物，在民间作为杀虫植物，有“菜虫药”之称，其主要活性成分雷公藤生物碱和雷公藤甲素既有杀虫活性又有医用活性（李琰等，2012；Brinker et al．，2005；Liu，201...雷公藤（Tripterygium wilfordii）不仅是一种药用植物，还是一种有毒植物，在民间作为杀虫植物，有“菜虫药”之称，其主要活性成分雷公藤生物碱和雷公藤甲素既有杀虫活性又有医用活性（李琰等，2012；Brinker et al．，2005；Liu，2011），临床上常用来治疗风湿性关节炎、肿瘤、免疫抑制性疾病（仲剑等，2009；Johnson et al．，2011；Huang et al．，2012）。展开更多
基金supported by the Youth Talent Project of Science and Technology Department, Fujian Province (No. 2007F3017)the Research Project of the Forestry Department, Fujian Province (Minlin 2004 Kehan No. 8)
文摘In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wilfordii Hook f.. Juvenile shoots from one-year-old elite plants were used as the source of explants. New axillary shoots were obtained after 30 days of culture on a MS medium supplemented with BAP （2.0 mg.L^-1） and NAA （0.1 mg.L^-1）. The optimal multiplication medium was a modified MS medium supplemented with BAP （1.0 mg.L^-1） and NAA （0.1 mg.L^-1）. This yielded a multiplication rate of 2.4 for each subculture. Slightly more than 92% of shoots rooted when cultured on a modified MS medium containing IBA （0.2 mg.L^-1） and activated charcoal （0.5 mg.L^-1）. Activated charcoal promoted both a strong and a high rooting rate during the rooting phase. Plantlets were transferred to pots for a short acclimatization stage in a greenhouse where 95% of the plantlets survived. This highly reproducible procedure can be adopted for large-scale propagation of T. wilfordii.
文摘雷公藤（Tripterygium wilfordii）不仅是一种药用植物，还是一种有毒植物，在民间作为杀虫植物，有“菜虫药”之称，其主要活性成分雷公藤生物碱和雷公藤甲素既有杀虫活性又有医用活性（李琰等，2012；Brinker et al．，2005；Liu，2011），临床上常用来治疗风湿性关节炎、肿瘤、免疫抑制性疾病（仲剑等，2009；Johnson et al．，2011；Huang et al．，2012）。
