In recent years,with the increase of millet market demand and the promotion and application of millet free(less)thinning technology in Jincheng City,the planting area of millet is stabilized at about 10000 hm^(2).Howe...In recent years,with the increase of millet market demand and the promotion and application of millet free(less)thinning technology in Jincheng City,the planting area of millet is stabilized at about 10000 hm^(2).However,in the process of planting,millet farmers have not paid enough attention to the control of Oulema tristis Herbst,and the damage caused by the pest is aggravating year by year,causing certain loss to millet farmers.Based on the investigation and analysis of the occurrence of O.tristis,the prevention and control suggestions are put forward,and good results have been obtained.展开更多
Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates ...Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates tightly clustered into one or the other of two groups that diverged at 12%. Results suggested that certain genotypes of the fungus, that grouped together, were able to infect moth larvae while others did not. A fragment of 760 bp, which presents high homology with a host-adaptation related protein coding gene, distinguished between aggressive and non-aggressive isolates. Neither mycelial growth nor sporulation rate or presence of known virulence genes was correlated with mortality values. Some isolates, including the most aggressive isolate ARSEF2518, were compatible with deltamethrin. Deltamethrin treatment killed all the larvae after seven days whereas fungal and mixed treatments respectively reached the same mortality after 28 and 21 days.展开更多
文摘In recent years,with the increase of millet market demand and the promotion and application of millet free(less)thinning technology in Jincheng City,the planting area of millet is stabilized at about 10000 hm^(2).However,in the process of planting,millet farmers have not paid enough attention to the control of Oulema tristis Herbst,and the damage caused by the pest is aggravating year by year,causing certain loss to millet farmers.Based on the investigation and analysis of the occurrence of O.tristis,the prevention and control suggestions are put forward,and good results have been obtained.
文摘Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates tightly clustered into one or the other of two groups that diverged at 12%. Results suggested that certain genotypes of the fungus, that grouped together, were able to infect moth larvae while others did not. A fragment of 760 bp, which presents high homology with a host-adaptation related protein coding gene, distinguished between aggressive and non-aggressive isolates. Neither mycelial growth nor sporulation rate or presence of known virulence genes was correlated with mortality values. Some isolates, including the most aggressive isolate ARSEF2518, were compatible with deltamethrin. Deltamethrin treatment killed all the larvae after seven days whereas fungal and mixed treatments respectively reached the same mortality after 28 and 21 days.
