目的:分析比较石蒜属和近缘属植物中国水仙trnL基因的部分序列和trnL-trnF间隔区的完整序列,为石蒜属物种鉴别和种间亲缘关系分析提供分子佐证。方法:采用PCR产物直接双向测序法,用Clustal X 1.81,MEGA 2.0软件进行序列分析,采用最大简...目的:分析比较石蒜属和近缘属植物中国水仙trnL基因的部分序列和trnL-trnF间隔区的完整序列,为石蒜属物种鉴别和种间亲缘关系分析提供分子佐证。方法:采用PCR产物直接双向测序法,用Clustal X 1.81,MEGA 2.0软件进行序列分析,采用最大简约法(maximum parsimony,MP)构建系统发育树。结果:所测物种的序列全长在905～1036 bp,经排序后,两端切平,得到886 bp的整齐序列,所测物种的核苷酸变异位点14个,信息位点10个,主要在trnL内含子区,这些位点可用于区分石蒜属物种;4个碱基"ATAT"缺失/插入是区别石蒜属与其近缘植物水仙的显著特征。重建的系统发育树表明:供试的石蒜属物种聚成3类,除稻草石蒜、忽地笑、香石蒜的系统位置有所不同之外,与经典分类结果基本相符。水仙属2个种形成1个单系类群,表明属间trnL-F序列差异明显。结论:分子系统树支持安徽石蒜是长筒石蒜的变种或杂交种观点,换锦花可能是玫瑰石蒜、红蓝石蒜的杂交母本。石蒜属种间trnL-F序列存在一定变异,是物种鉴别的有效分子标记。展开更多
The molecular phylogeny of the Lardizabalaceae is reconstructed based on chloroplast trn L_F sequences alone and combined trn L_F and rbc L sequences. The phylogenetic topologies agree well with Qin's and...The molecular phylogeny of the Lardizabalaceae is reconstructed based on chloroplast trn L_F sequences alone and combined trn L_F and rbc L sequences. The phylogenetic topologies agree well with Qin's and Takhtajan's tribal classification in both analyses. Decaisneae and Sinofranchetieae are basal clades in the phylogenetic trees and external to all other taxa in the family. Lardizabaleae consisting of Boquila and Lardizabala are well supported in both trn L_F (100%) analysis and trn L_F and rbc L combined analysis (99%). Tribe Akebieae are strongly supported by a bootstrap value of 100% in both trn L_F analysis and trn L_F and rbc L combined analysis. However, the new genus Archakebia is nested within the genus Akebia in the trn L_F trees. In the combined trees, Archakebia is sister to Akebia with high bootstrap support. The inter_relationships among three closely related genera Parvatia , Holboellia and Stauntonia are still problematic. P. brunoniana ssp. elliptica is sister to H. latifolia in both analyses with low bootstrap support. H. parviflora is nested within the Stauntonia and sister to S. cavalerieana . Therefore, these three genera of tribe Akebieae may not be monophylytic and their generic boundary and delimitation need to be further studied, by exploring more molecular data, together with more morphological characters.展开更多
Phylogenetic relationships of the 'higher' hamamelids were estimated based on nucleotide sequences of the non-coding region of chloroplast trnL-F. Twenty-five species were sampled, representing 24 genera of al...Phylogenetic relationships of the 'higher' hamamelids were estimated based on nucleotide sequences of the non-coding region of chloroplast trnL-F. Twenty-five species were sampled, representing 24 genera of all eight families of the 'higher' hamamelids. A parsimony analysis of data set indicated that the 'higher' hamamelids formed a strongly supported clade with 100% bootstrap value in the strict consensus tree. Nothofagus was the basal lineage and the Fagaceae was sister to a well-supported core 'higher' hamamelids clade containing Myricaceae, Rhoipteleaceae, Juglandaceae, Casuarinaceae, Ticodendraceae and Betulaceae. Three clades were recognized in the core ' higher' hamamelids: (1) Casuatina ( Ticodendron, ( Betu-laceae)), (2) Juglandaceae-Rhoipteleaceae, and (3) Myricaceae. Compared with the previous studies, familial relationships of the 'higher' hamamelids were better resolved.展开更多
文摘The molecular phylogeny of the Lardizabalaceae is reconstructed based on chloroplast trn L_F sequences alone and combined trn L_F and rbc L sequences. The phylogenetic topologies agree well with Qin's and Takhtajan's tribal classification in both analyses. Decaisneae and Sinofranchetieae are basal clades in the phylogenetic trees and external to all other taxa in the family. Lardizabaleae consisting of Boquila and Lardizabala are well supported in both trn L_F (100%) analysis and trn L_F and rbc L combined analysis (99%). Tribe Akebieae are strongly supported by a bootstrap value of 100% in both trn L_F analysis and trn L_F and rbc L combined analysis. However, the new genus Archakebia is nested within the genus Akebia in the trn L_F trees. In the combined trees, Archakebia is sister to Akebia with high bootstrap support. The inter_relationships among three closely related genera Parvatia , Holboellia and Stauntonia are still problematic. P. brunoniana ssp. elliptica is sister to H. latifolia in both analyses with low bootstrap support. H. parviflora is nested within the Stauntonia and sister to S. cavalerieana . Therefore, these three genera of tribe Akebieae may not be monophylytic and their generic boundary and delimitation need to be further studied, by exploring more molecular data, together with more morphological characters.
文摘Phylogenetic relationships of the 'higher' hamamelids were estimated based on nucleotide sequences of the non-coding region of chloroplast trnL-F. Twenty-five species were sampled, representing 24 genera of all eight families of the 'higher' hamamelids. A parsimony analysis of data set indicated that the 'higher' hamamelids formed a strongly supported clade with 100% bootstrap value in the strict consensus tree. Nothofagus was the basal lineage and the Fagaceae was sister to a well-supported core 'higher' hamamelids clade containing Myricaceae, Rhoipteleaceae, Juglandaceae, Casuarinaceae, Ticodendraceae and Betulaceae. Three clades were recognized in the core ' higher' hamamelids: (1) Casuatina ( Ticodendron, ( Betu-laceae)), (2) Juglandaceae-Rhoipteleaceae, and (3) Myricaceae. Compared with the previous studies, familial relationships of the 'higher' hamamelids were better resolved.
