Subtropical forest macro-decomposers rapidly transfer litter carbon and nitrogen into soil mineral-associated organic matter

Background:Forest soils in tropical and subtropical areas store a significant amount of carbon.Recent framework to assess soil organic matter(SOM)dynamics under evolving global conditions suggest that dividing bulk SOM into particulate and mineral-associated organic matter(POM vs.MAOM)is a promising method for identifying how SOM contributes to reducing global warming.Soil macrofauna,earthworms,and millipedes have been found to play an important role in facilitating SOM processes.However,how these two co-existing macrofaunae impac the litter decomposition process and directly impact the formation of POM and MAOM remains unclear.Methods:Here,we set up a microcosm experiment,which consisted of 20 microcosms with four treatments earthworm and litter addition(E),millipedes and litter addition(M),earthworm,millipedes,and litter addition(E+M),and control(only litter addition)in five replicates.The soil and litter were sterilized prior to beginning the incubation experiment to remove any existing microbes.After incubating the samples for 42 days,the litte properties(mass,C,and N contents),soil physicochemical properties,as well as the C and N contents,and POM and MAOM^(13)C abundance in the 0–5 and 5–10 cm soil layers were measured.Finally,the relative influences o soil physicochemical and microbial properties on the distribution of C and N in the soil fractions were analyzed Results:The litter mass,C,and N associated with all four treatments significantly decreased after incubation especially under treatment E+M(litter mass:-58.8%,litter C:-57.0%,litter N:-75.1%,respectively),while earthworm biomass significantly decreased under treatment E.Earthworm or millipede addition alone showed no significant effects on the organic carbon(OC)and total nitrogen(TN)content in the POM fraction,but join addition of both significantly increased OC and TN regardless of soil depth.Importantly,all three macrofauna treatments increased the OC and TN content and decreased the^(13)C abundance in the MAOM fraction.More than65%of the total variations in the distribution of OC and TN throughout the two fractions can be explained by a combination of soil physicochemical and microbial properties.Changes in the OC distribution in the 0–5 cm soi layer are likely due to a decrease in soil pH and an increase in arbuscular mycorrhizal fungi(AMF),while those in the 5–10 cm layer are probably caused by increases in soil exchangeable Ca and Mg,in addition to fungi and gram-negative(GN)bacteria.The observed TN distribution changes in the 0–5 cm soil likely resulted from a decrease in soil pH and increases in AMF,GN,and gram-negative(GP)bacteria,while TN distribution changes in the 5–10 cm soil could be explained by increases in exchangeable Mg and GN bacteria.Conclusions:The results indicate that the coexistence of earthworms and millipedes can accelerate the litte decomposition process and store more C in the MAOM fractions.This novel finding helps to unlock the processe by which complex SOM systems serve as C sinks in tropical forests and addresses the importance of soil mac rofauna in maintaining C-neutral atmospheric conditions under global climate change.

Simulation and Analysis about the Effects of Geopotential Height Anomaly in Tropical and Subtropical Region on Droughts or Floods in the Yangtze River Valley and North China

Previous study comes to the conclusion： based on the anomalies of the South Asian high （SAH）, 100-hPa geopotential height, and 100-hPa circulation over tropical and subtropical regions, we can predict precipitation anomaly in the Yangtze River Valley and North China. To test its validity, a series of experiments have been designed and operated, which include controlled experiment, sensitivity experiment （which has added anomalies into 100-hPa geopotential height and wind field）, and four-composite experiments. Experiments based on the composed initial field such as EPR-CF, EPR-CD, EPR-HF, and EPR-HD, can reproduce the floods or droughts in the Yangtze River Valley and North China. It suggests that anomalies of the SAH, 100- hPa geopotential height, and circulation over tropical and subtropical regions may probably imply summer precipitation anomalies in the two regions. Sensitivity experiment results show that anomalies of the SAH, 100-hPa geopotential height, and southwest flow in the previous period is a signal of droughts or floods for the following summer in the Yangtze River Valley and North China. And it is also one of the factors that have impact on summer precipitation anomaly in the two regions. Positive anomaly of 100-hPa geopotential height and the anomalous intensifying of the SAH and southwest flow will induce floods in the Yangtze River Valley and droughts in North China; while negative anomaly of 100-hPa geopotential height and anomalous weakening of the SAH and southwest flow will induce droughts in the Yangtze River Valley and floods in North China.