Dynamics of soil inorganic phosphorus fractions at aggregate scales in a chronosequence of Chinese fir plantations

Successive cultivation of Chinese fir(Cunninghamia lanceolata) would markedly affect the distribution and accumulation of soil inorganic phosphorus(Pi).However,how different chronosequence phases of Chinese fir plantations exerting influences on the quality and quantity of soil Pi fractions in aggregate-scale remain poorly understood. This study researched the dynamic changes of aggregate-related Pi fractions encompassing occluded-P(O-P), aluminum-bound P(Al-P), iron-bound P(Fe-P), and calcium-bound P(Ca-P) in topsoil(0-20 cm) from different stand aged(9-, 17-, and 26-yr) Chinese fir plantations and one nearby abandoned land(CK) in Rongshui County,Guangxi, China. In this study, soil aggregates were classified into micro-aggregates(< 0.25 mm), small macro-aggregates(1-0.25 mm), medium macroaggregates(2-1 mm), and large macro-aggregates(> 2 mm) by one wet-sieving process. As the primary aggregate fractions correlated with better soil aggregate stability, the large macro-aggregates took the highest proportion in all aggregate sizes regardless of various stand ages of Chinese fir plantations. Besides, the 17-yr plantations of Chinese fir displayed the highest stability of aggregates structure. Compared with CK, all four soil Pi fractions from three different stand ages of Chinese fir plantations generally showed increasing trends.Irrespective of chronosequence phases, Al-P was mainly carried by small macro-aggregates. O-P showed the opposite tendency to Al-P, which had the lowest content in small macro-aggregates. Fe-P and Ca-P showed an even distribution in all aggregates.The contribution rates and stocks of each Pi fraction exhibited close relevance to the content of soil aggregates. As revealed from the results, planting of Chinese fir before 17-yr was beneficial to prompt the formation of large macro-aggregates and the level of soil P. But after 17-yr, successive monoculture planting of Chinese fir would reduce the stability of soil aggregates and render the losses of soil P. The dynamics of soil total phosphorous(TP) and Pi fractions contents were highly related to the stand ages of Chinese fir plantations, but less related to the distribution of soil aggregate sizes. As the major carriers for soil P stocks, the large macro-aggregates played a vital role in the cycles and reserves of soil P.

Effect of long-term fertilization on phosphorus fractions in different soil layers and their quantitative relationships with soil properties

Investigating the dynamics and distribution of soil phosphorus(P) fractions can provide a basis for enhancing P utilization by crops. Four treatments from a 29-year long-term experiment in black soil with maize cropping were involved in this study: no fertilizer(CK), inorganic nitrogen and potassium(NK), inorganic nitrogen, phosphorus, and potassium(NPK), and NPK plus manure(NPKM). We analyzed soil P fractions in different soil layers using a modified Hedley sequential method. The long-term NPKM treatment significantly increased total P by 0.6–1.6 times in the different soil layers. The Olsen-P concentration far exceeded the environmental threshold for soil Olsen-P(50.6 mg kg) in the NPKM treatment in the 0–60 cm soil profile. Moreover, the concentrations and proportion of labile and partially labile inorganic P(Pi) fractions(i.e., Na HCO-extracted Pi, Na OH-extracted Pi, and dilute HClextracted Pi) to the sum of all P fractions(Pt) in the 0–60 cm soil profile were higher in the NPKM treatment than in the NPK treatment, indicating that manure could promote the transformation of non-labile into more labile forms of P in soil, possibly by manure reducing P fixation by soil particles. Soil organic matter, Mehlich-3 extractable iron(Fe), and organic-bound aluminum were increased by fertilization, and were the main factors influencing the differences in the P fractions in the 0–20 cm soil layer. Soil mineral components, i.e., free Fe oxide and Ca CO, were the main factors influencing the P fractions in the subsoil. The soil P transformation process varied with soil layer and fertilization. Application of manure fertilizer can increase the labile(Olsen) P concentrations of the various soil layers, and thus should reduce the mineral P fertilizer requirement for crop growth and reduce potential environmental damage.

Characteristics of inorganic phosphorus fractions and their correlations with soil properties in three non-acidic soils

Understanding the characteristics and influences of various factors on phosphorus(P)fractions is of significance for promoting the efficiency of soil P.Based on long-term experiments on black soil,fluvo-aquic soil,and loess soil,which belong to Phaeozems,Cambisols,and Anthrosols in the World Reference Base for Soil Resources(WRB),respectively,five fertilization practices were selected and divided into three groups:no P fertilizer(CK/NK),balanced fertilizer(NPK/NPKS),and manure plus mineral fertilizer(NPKM).Soil inorganic P(Pi)fractions and soil properties were analyzed to investigate the characteristics of the Pi fractions and the relationships between Pi fractions and various soil properties.The results showed that the proportion of Ca_(10)-P in the sum of total Pi fractions was the highest in the three soils,accounting for 33.5%in black soil,48.8%in fluvo-aquic soil,and 44.8%in loess soil.Long-term fertilization practices resulted in periodic changes in soil Pi accumulation or depletion.For black soil and fluvo-aquic soil,the Pi accumulation was higher in the late period(10–20 years)of fertilization than in the early period(0–10 years)under NPK/NPKS and NPKM,whereas the opposite result was found in loess soil.The Pi accumulation occurred in all Pi fractions in black soil;mainly in Ca_(8)-P,Fe-P,and Ca_(10)-P in fluvo-aquic soil;and in Ca_(2)-P,Ca_(8)-P,and O-P in loess soil.Under CK/NK,the soil Pi was depleted mainly in the early period in each of the three soils.In addition to the labile Pi(Ca_(2)-P)and moderately labile Pi(Ca_(8)-P,Fe-P,Al-P),the Ca_(10)-P in black soil and fluvo-aquic soil and O-P in loess soil could also be used by crops.Redundancy analysis showed that soil properties explained more than 90%of the variation in the Pi fractions in each soil,and the explanatory percentages of soil organic matter(SOM)were 43.6%in black soil,74.6%in fluvo-aquic,and 38.2%in loess soil.Consequently,decisions regarding the application of P fertilizer should consider the accumulation rate and the variations in Pi fractions driven by soil properties in non-acidic soils.

Does Soil Disturbance Affect Soil Phosphorus Fractions?

Increased turnover of organic matter as a result of soil disturbance (e.g. by soil tillage) is described in principle, but the direct influence of soil disturbance on soil P turnover especially for organic farming systems has not been sufficiently proven. The objective of the study was to evaluate the short term effect of soil disturbance on different soil P fractions in a soil shaking experiment. Four soils were incubated for 10 days in the dark with three different disturbance treatments: 1) no disturbance, 2) overhead shaking for 2 h at the beginning of the experiment and 3) continuous overhead shaking at 5 r. p. m. The four investigated soils were: 1) a silty loam soil with long term bio-compost application and 2) the corresponding soil without bio-compost application, 3) a long-term organically managed clay loam soil and 4) a clay loam soil with long time application of pig manure, all not and from Baden-Württemberg, Germany. We determined NaHCO3-, NaOH- and H2SO4-extractable inorganic and organic P fractions (Pi and Po, resp.) in a sequential extraction. Furthermore, the potentially plant available P as Calcium-acetate-lactate-extractable P (CAL-P) and P extractable by electro-ultra-filtration (EUF-P), and aqua regia extractable total P (PT) were determined. Furthermore, we determined microbial biomass carbon (MBC), nitrogen (MBN) and phosphorus (MBP), and acid phosphatase activity in soil. The organically managed soil had the highest PT contents (1300 mg·kg-1). The soil with pig manure application had the smallest potentially labile P fractions (NaHCO3-Pi and -Po and NaOH-Pi). The ecologically managed soil had the biggest organic P fractions (114 mg·kg-1 NaHCO3-Po and 463 mg·kg-1 NaOH-Po), but, this soil was the lowest in CAL-P (5 mg·kg-1). Short term soil disturbance had effects on labile organic P fractions of two of the four analyzed soils, but inorganic P was rather unaffected. In the compost amended COMP(+) soil, there was an incorporation of P from the less available NaOH-P fractions into the more available NaHCO3-Po fraction. However, if taking all investigated soils and treatments into account, the effects of soil disturbance were limited and not consistent.

Changes in Phosphorus Fractions, pH, and Phosphatase Activity in Rhizosphere of Two Rice Genotypes

A rhizobox experiment with two phosphorus (P) treatments, zero-P (0 mg P kg-1) and plus-P (100 mg P kg-1) as Ca(H2PO4)2·H2O, was conducted to study the chemical and biochemical properties in the rhizosphere of two rice genotypes (cv. Zhongbu 51 and Pembe) different in P uptake ability and their relationship with the depletion of soil P fractions. Plant P uptake, pH, phosphatase activity, and soil P fractions in the rhizosphere were measured. Both total dry weight and total P uptake of Pembe were significantly (P < 0.05) higher than those of Zhongbu 51 in the zero-P and plus-P treatments. Significant depletions of resin-Pi, NaHCO3-Pi, NaHCO3-Po, and NaOH-Pi, where Pi stands for inorganic P and Po for organic P, were observed in the rhizosphere of both Zhongbu 51 and Pembe under both P treatments. Pembe showed a greater ability than Zhongbu 51 in depleting resin-Pi, NaHCO3-Pi, NaHCO3-Po, NaOH-Pi, and NaOH- Po in the rhizosphere. HCl-Pi and residual-P were not depleted in the rhizosphere of both genotypes, regardless of P treatments despite significant acidification in the rhizosphere of Pembe under zero-P treatment. Higher acid phosphatase (AcPME) activity and alkaline phosphatase (AlPME) activity were observed in the rhizosphere of both Zhongbu 51 and Pembe compared to the corresponding controls without plant. AcPME activity was negatively (P < 0.01) correlated to NaHCO3-Po concentration in the rhizosphere of both Zhongbu 51 and Pembe, suggesting that AcPME was associated with the mineralization of soil organic P.

Phosphorus fractions of fertiliser-derived P in an allophanic soil under Pinus radiata seedlings grown with broom and ryegrass

Changes in phosphorus （P） fractions in a P deficient allophanic soil under P. radiata seedlings grown with broom （Cytisus scoparius L.） and ryegrass （Lolium multiflorum） in pots were studied 14 months after the application of triple superphosphate at the rates of 0, 50, and 100 pg.g^-1, to determine the fate of fertiliser-derived P in the rhizosphere soils. Application of P fertiliser increased NaOH-Pi, NaOH-Po, and H2SO4-Pi concentrations in the soil, but decreased the residual-P concentration. The resin-Pi concentration, which is ex- tremely low in this soil （1 to μgg^-1 ）, remained the same. The majority of the added fertiliser P was however recovered in the NaOH-Pi fraction （40%-49%）. This is due to the high P fixation in this soil （92%）. The second highest P recovery was in NaOH-Po fraction （7%-19%）. Under P deficient condition or addition at the rate of 0 μg.g^-6, the NaOH-Pi concentration in the radiata rhizosphere soil was lower than that in the bulk soil and broom and grass rhizosphere soils. This may be due to higher oxalate production by the roots and mycorrhiza under P deficient conditions which released some ＆the P fixed to the soils in the rhizosphere, which needs to be tested in future studies.

Phosphorus fractions of fertiliser-derived P in an allophanic soil under Pinus radiata seedlings grown with broom and ryegrass

Changes in phosphorus (P) fractions in a P deficient allophanic soil under P. radiata seedlings grown with broom (Cytisus scoparius L.) and ryegrass (Lolium multiflorum) in pots were studied 14 months after the application of triple superphosphate at the rates of 0, 50, and 100 μg·g-1, to determine the fate of fertiliser-derived P in the rhizosphere soils. Application of P fertiliser increased NaOH-Pi, NaOH-Po, and H2SO4-Pi concentrations in the soil, but decreased the residual-P concentration. The resin-Pi concentration, which is extremely low in this soil (1 to 3 μg·g-1 ), remained the same. The majority of the added fertiliser P was however recovered in the NaOH-Pi fraction (40%?49%). This is due to the high P fixation in this soil (92%). The second highest P recovery was in NaOH-Po fraction (7%?19%). Under P deficient condition or addition at the rate of 0 μg·g-1, the NaOH-Pi concentration in the radiata rhizosphere soil was lower than that in the bulk soil and broom and grass rhizosphere soils. This may be due to higher oxalate production by the roots and mycorrhiza under P deficient conditions which released some of the P fixed to the soils in the rhizosphere, which needs to be tested in future studies.

Aging Effects of Organic and Inorganic Fertilizers on Phosphorus Fractionation in a Calcareous Sandy Loam Soil

In most arid and semiarid soils, naturally occurring phosphorus(P) is a major yield-limiting plant nutrient. In this study, to investigate the effects of organic(OP) and inorganic P(IP) sources on P fractionation, a calcareous sandy loam alkaline soil was fertilized with OP and IP fertilizers at low(80 mg P kg^(-1) soil) and high(160 mg P kg^(-1) soil) application rates. Three combinations of OP and IP(i.e., 75% OP + 25% IP, 50% OP + 50% IP, and 25% OP + 75% IP) were applied at low and high application rates,respectively, followed by soil aging for 21 d. Soil samples were collected after 1, 2, 3, 7, and 21 d and subjected to sequential extraction to analyze soluble and exchangeable, Fe-and Al-bound, Ca-bound, and residual P fractions. The soluble and exchangeable P fraction significantly increased up to 24.3%, whereas the Ca-bound fraction decreased up to 40.7% in the soils receiving 75% OP + 25% IP and 50% OP + 50% IP, respectively, compared with the control(receiving no P fertilizer). However, the transformation of P fractions was influenced by aging time. Addition of P sources caused instant changes in different P fractions, which then tended to decline with aging time. Change in soil p H was the limiting factor in controlling P availability. At high application rate, the OP source significantly increased soil P availability compared with the IP source with soil aging. Depending on P fractionation, a proper combination of OP and IP fertilizers, as long-term slow and instant P-releasing sources for plant uptake, respectively, may be a sustainable strategy to meet crop P requirements in the arid and semiarid soils.

Fractions and Bioavailability of Soil Inorganic Phosphorus in the Loess Plateau of China under Different Vegetations

Plants play an important role in soil phosphorus nutrition. However, the effect of plants on phosphorus nutrition in soils of the Loess Plateau of China is not well understood. This study was conducted to reveal the relationships between plants and phosphorus＇ fractions and availability in the Loess Plateau of China. Twenty-two plant communities were surveyed and soil samples under different plant canopies were collected for the determination of soil properties and inorganic phosphorus fractionation. The results showed that Leguminosae and Lilaceae reduced pH and increased organic matter, cation exchange capacity, total and Olsen phosphorus in soils under their canopies, while Labiatae and Rosaceae increased pH and decreased organic matter, cation exchange capacity, total and Olsen phosphorus in soils under their canopies. The contents of Ca2P, CasP, AI-P and Fe-P were highly related with soil Oisen phosphorus. They were all higher in soils under Leguminosae and Lilaceae and lower in softs under Labiatae and Rosaceae. The results of this study indicate that Leguminosae and Lilaceae improved phosphorus nutrition in soils, yet Labiatae and Rosaceae impeded the improvement of phosphorus nutrition in soils under their canopies, which will be of more help to instruct vegetation restoration in the region and provide information for soil development.

Subtropical forest macro-decomposers rapidly transfer litter carbon and nitrogen into soil mineral-associated organic matter

Background:Forest soils in tropical and subtropical areas store a significant amount of carbon.Recent framework to assess soil organic matter(SOM)dynamics under evolving global conditions suggest that dividing bulk SOM into particulate and mineral-associated organic matter(POM vs.MAOM)is a promising method for identifying how SOM contributes to reducing global warming.Soil macrofauna,earthworms,and millipedes have been found to play an important role in facilitating SOM processes.However,how these two co-existing macrofaunae impac the litter decomposition process and directly impact the formation of POM and MAOM remains unclear.Methods:Here,we set up a microcosm experiment,which consisted of 20 microcosms with four treatments earthworm and litter addition(E),millipedes and litter addition(M),earthworm,millipedes,and litter addition(E+M),and control(only litter addition)in five replicates.The soil and litter were sterilized prior to beginning the incubation experiment to remove any existing microbes.After incubating the samples for 42 days,the litte properties(mass,C,and N contents),soil physicochemical properties,as well as the C and N contents,and POM and MAOM^(13)C abundance in the 0–5 and 5–10 cm soil layers were measured.Finally,the relative influences o soil physicochemical and microbial properties on the distribution of C and N in the soil fractions were analyzed Results:The litter mass,C,and N associated with all four treatments significantly decreased after incubation especially under treatment E+M(litter mass:-58.8%,litter C:-57.0%,litter N:-75.1%,respectively),while earthworm biomass significantly decreased under treatment E.Earthworm or millipede addition alone showed no significant effects on the organic carbon(OC)and total nitrogen(TN)content in the POM fraction,but join addition of both significantly increased OC and TN regardless of soil depth.Importantly,all three macrofauna treatments increased the OC and TN content and decreased the^(13)C abundance in the MAOM fraction.More than65%of the total variations in the distribution of OC and TN throughout the two fractions can be explained by a combination of soil physicochemical and microbial properties.Changes in the OC distribution in the 0–5 cm soi layer are likely due to a decrease in soil pH and an increase in arbuscular mycorrhizal fungi(AMF),while those in the 5–10 cm layer are probably caused by increases in soil exchangeable Ca and Mg,in addition to fungi and gram-negative(GN)bacteria.The observed TN distribution changes in the 0–5 cm soil likely resulted from a decrease in soil pH and increases in AMF,GN,and gram-negative(GP)bacteria,while TN distribution changes in the 5–10 cm soil could be explained by increases in exchangeable Mg and GN bacteria.Conclusions:The results indicate that the coexistence of earthworms and millipedes can accelerate the litte decomposition process and store more C in the MAOM fractions.This novel finding helps to unlock the processe by which complex SOM systems serve as C sinks in tropical forests and addresses the importance of soil mac rofauna in maintaining C-neutral atmospheric conditions under global climate change.

The distribution and availability of phosphorus fractions in restored cut slopes soil aggregates:a case study of subalpine road,Southwest China

The distribution and availability of phosphorus(P)fractions in restored cut slope soil aggregates,along altitude gradients,were analyzed.Samples were collected at 3009,3347,3654 and 3980 m of altitude.We examined soil aggregates total phosphorus(TP),available phosphorus(AP)and phosphorus activation coefficient(PAC),and discovered that there was no significant difference in TP levels between all four altitudes samples(p>0.05).However,there was a significant difference in AP at 3009,3347 and 3980 m of altitude(p<0.05).At the altitudes of 3009,3347 and 3654 m,the AP accumulation in small size aggregates was more advantageous.Overall,PAC dropped steadily as soil aggregates sizes increased,as shown:PAC(3654 m)>PAC(3347 m)>PAC(3009 m)>PAC(3980 m).In all particle size soil aggregates,the distribution of the P fractions was as follows:total inorganic phosphorus(TPi)>total organic phosphorus(TPo)>residual phosphorus(R-P),at 3009,3347 and 3654 m,but a different registry was observed at 3980 m of altitude:TPo>TPi>R-P.Through correlation and multiple stepwise regression analysis,it was concluded that active NaHCO_(3)-Pi was the main AP source.It was also suggested that more attention should be given to the ratio of small particle size aggregates to increase soil AP storage.In order to improve the activation capacity and supply of soil P,along with promotion of the healthy development of soil ecosystem on slope land,it was suggest that inorganic P fertilizer and P activator could be added to soil at both low(3009 m)and high altitudes(3980 m).

Impacts of soil properties on phosphorus adsorption and fractions in purple soils

Information on phosphorus(P) adsorption and its impacts on the redistribution of the P fraction in soil profiles are important for environmental management under intensive agricultural practices.To clarify the dominant factors influencing soil phosphorus adsorption in an Entisol(locally known as purple soil), P adsorption experiments were conducted in Sichuan Basin of southwestern China for cropland and woodland soils with acidic, neutral and calcareous origins throughout their profile. After various doses of P were added during incubation experiments, soil P fractions were also analyzed. The results showed that there were no significant differences in Fe-oxides and P adsorption along the vertical gradients. Agricultural practices and lower p H conditions reduced the P adsorption capacity of purple soils throughout the soil profiles. For acidic and neutral purple soil profiles, the P adsorption capability was mainly influenced by Fe-oxides and soil texture. Ca-bound P and Fe-Al-bound P represented the majority of the total inorganic P of calcareous soils.There was a saturation of adsorption capacity by sesquioxide and a high risk of dissoluble reactive P(NH_4 Cl-P) being released out of the soil profile in acidic and neutral purple soils after the greatest P addition, indicated by the higher proportions of NH_4 Cl-P(over 40%) and decreasing Fe-Al-P fraction.P fractions migrated with greater difficulty in calcareous purple soil profiles as Ca-P fraction peaked over 65% when adding a P dose at or greater than 80 g P kg^(-1), indicating the high potential of P adsorption.The X-Ray Diffraction analysis also verified the formation of brushite. Adaptive management practices should be designed to alleviate P losses for acidic and neutral purple soils.

Surface Reactivity in Tropical Highly Weathered Soils and Implications for Rational Soil Management

Highly weathered soils are distributed in the humid and wet-dry tropics, as well as in the humid subtropics. As a result of strong weathering, these soils are characterized by low activity clays, which develop variable surface charge and related specific properties. Surface reactions regarding base exchange and soil acidification, heavy metal sorption and mobility, and phosphorus sorption and availability of the tropical highly weathered soils are reviewed in this paper.Factors controlling surface reactivity towards cations and anions, including ion exchange and specific adsorption processes, are discussed with consideration on practical implications for rational management of these soils. Organic matter content and pH value are major basic factors that should be controlled through appropriate agricultural practices, in order to optimise favorable effects of colloid surface properties on soil fertility and environmental quality.

Effects of Biochar and Wood Vinegar on Labile Phosphorus Pool in Soda Saline-Alkaline Soil

A pot experiment was conducted to research the effect of biochar and wood vinegar on labile phosphorus fractions in saline-alkali soil.There were eight treatments,including CK(0 kg•hm-2 biochar+0 kg•hm-2 wood vinegar),C1(0.6 t•hm-2 biochar),C2(0.6 t•hm-2 wood vinegar),C3(1.2 t•hm-2 wood vinegar),C4(1.8 t•hm-2 wood vinegar),C5(0.6 t•hm-2 biochar+0.6 t•hm-2 wood vinegar),C6(0.6 t•hm-2 biochar+1.2 t•hm-2 wood vinegar),and C7(0.6 t•hm-2 biochar+1.8 t•hm-2 wood vinegar).The results showed that biochar without wood vinegar and the co-application of biochar and wood vinegar significantly increased soil total phosphorus content.Meanwhile,compared with CK,all of treatments increased resin phosphorus and sodium bicarbonate-extracted inorganic phosphorus(NaHCO3-Pi)contents in saline-alkali soil.Especially,the contents of resin phosphorus and NaHCO3-Pi under C5,C6,and C7 treatments were higher than those of C2,C3,and C4 treatments,respectively,indicating that the increases of labile phosphorus contents under the co-application of biochar and wood vinegar were better than those of the alone application of biochar and wood vinegar.Each treatment increased the proportion of labile phosphorus pool in saline-alkali soil and the proportion of labile phosphorus pool increased with the increase of the amount of wood vinegar.In addition,the application of biochar and wood vinegar increased the 100-grain weight of rice,and C6 treatment had the best effect,increasing the 100-grain weight by 134.35%.Therefore,the application of biochar and wood vinegar in saline-alkali soil could improve the soil phosphorus availability,increase the weight of rice grains,thereby realizing the resource utilization of agricultural waste and the sustainable development of agriculture.

Impact of Long-Term Fertilization on Phosphorus Status in Black Soil

A twelve-year field trial was carried out to determine the impact of long-term fertilization on phosphorus (P) distribu- tion in the soil profile, P balance, and the transformation and availability of soil P in a black soil (Phaeozem, FAO). Under a wheat-soybean-corn rotation, during twelve years where no fertilizer was applied, crops removed 60 and 81 mg P kg-1 soil in the control and NK treatment, respectively. About one third of the P absorbed by crops was originated from organic P. Ca2-P, Cag-P, Al-P and Fe-P were the main forms of inorganic P absorbed by crops. The surplus P from fertilization remained in the 0-20 cm soil layer and increased with the application rate of P. The combined application of NP or NPK increased available P to a lesser extent than the combined application of PK. Fertilizer P had mainly transformed to the Al-P, Fe-P, Ca2-P, and Cas-P forms. By using the P budget (X), changes in total P (Y1) and available P (Y2) in soils under the current cropping system could be predicted by the equations: Y1 = 0.02 + 1.01X and Y2 = 2.08 + 0.15X.

Effect of Continuous Vegetable Cultivation on Phosphorus Levels of Fluvo-Aquic Soils

Soil P status, inorganic P fractions, and P sorption properties were studiedusing sandy fluvo-aquic horticultural soils, which are high in organic matter content for vegetableproduction in comparison with a soil used for grain crop production in Zhengzhou, Henan Province,China P fractions, Olsen-P, and OM were determined at different depths in the soil profile andsorption isotherm experiments were performed Most P in excess of plant requirements accumulated inthe topsoil and decreased with soildepth. Total P, inorganic P, and OM concentrations increased withcontinued horticultural use Olsen-P concentrations in the 0-20 cm depth of horticultural soils were9 to 25 times higher than those of the grain crop soil. A linear transformation of the Langmuirequation showed that the P adsorption maximum (491.3 mg P kg^(-1)) and the maximum phosphatebuffering capacity (162.1 L kg^(-1)) for 80--100 cm were greater in the grain crop soil than thehorticultural soils. Thus, the most immediate concern with excess P were in areas where heavy Pfertilizer was used for vegetable crops and where soil P sorption capacities were low due to sandysoils and high organic matter content.

Field demonstration of reduction of lead availability in soil and cabbage (Brassica Chinensis L.) contaminated by mining tailings using phosphorus fertilizers

A field demonstration of reduction of lead availability in a soil and cabbage (Brassica Chinensis L.) contaminated by mining tailings, located in Shaoxing, China was carried out to evaluate the effects of applications of phosphorus fertilizers on Pb fractionation and Pb phytoavailability in the soil. It was found that the addition of all three P fertilizers including single super phosphate (SSP), phosphate rock (PR), and calcium magnesium phosphate (CMP) significantly decreased the percentage of water-soluble and exchangeable (WE) soil Pb and then reduced the uptake of Pb, Cd, and Zn by the cabbage compared to the control (CK). The results showed that the level of 300 g P/m2 soil was the most cost-effective application rate of P fertilizers for reducing Pb availability at the first stage of remediation, and that at this P level, the effect of WE fraction of Pb in the soil de- creased by three phosphorus fertilizers followed the order: CMP (79%)>SSP (41%)>PR (23%); Effectiveness on the reduction of Pb uptake by cabbage was in the order: CMP (53%)>SSP (41%)>PR (30%). Therefore our field trial demonstrated that it was effective and feasible to reduce Pb availability in soil and cabbage contaminated by mining tailings using P fertilizers in China and PR would be a most cost-effective amendment.

Soil phosphorus bioavailability assessed by XANES and Hedley sequential fractionation technique in a glacier foreland chronosequence in Gongga Mountain, Southwestern China

The primary productivity of terrestrial ecosystems is influenced by soil phosphorus bioavailability, which depends largely on chemical fractions of phosphorus. The sequential fractionation technique developed by Hedley et al. or its subsequent modification is a well-known method to determine soil phosphorus forms. Hedley sequential fractionation technique separates the phosphorus into fractions based on their different chemical solubilities in extractants with certain chemical properties. Recently, synchrotron-based X-ray absorption near edge structure(XANES) spectroscopy has been employed to measure soil phosphorus species directly and non-invasively. The XANES method provides information concerning local structure and chemical information of target elements at a molecular level. Thus, it can distinguish phosphorus fractions bound by metal oxides or hydroxides(such as Fe, Al, and Ca). In this present work, the phosphorus speciation of topsoil along a glacial foreland chronosequence in Gongga Mountain is determined using these two methods. The changes in soil phosphorus bioavailability along the 120-year-old chronosequence are assessed based on comparisons of the results obtained by these two methods. The results indicate that Hedley sequential fractionation technique shows a greater ability to determine soil bioavailable phosphorus(Resin-P and NaCHO3-P), while XANES is effective in distinguishing phosphorus bound by metal compounds. In the chronosequence, Ca- and Al-bound phosphorus were derived mainly from primary minerals, whose phosphorus contents decreased within 120 years of moraine weathering and soil development. The content of soil bioavailable phosphorus increased rapidly after 30 years since deglaciation. The increasing phosphorus bioavailability promoted the colonizing and primary succession vegetation.

热带胶园土壤磷素形态和生物有效性

采用Hedley的磷素分级方法研究热带胶园土壤磷的形态，结果表明、热带胶园土壤磷主要以ResidualP、NaOH－Po和NaOH－Pi形态存在；各种形态磷的损失质量分数均高于38％；其中ResidualP和NaOH－Po的损失量最高，分别占全磷损失量的52％和16％，它们是长期耕作条件下热带胶园土壤磷两种最重要的有效磷源。

Air-drying changes the distribution of Hedley phosphorus pools in forest soils

Hedley labile phosphorus(P)pools in soil tend to be several times larger than annual forest requirements,even in highly weathered soils characterized by P limitation.The discrepancy between plant and soil P status could be partly attributable to the frequently adopted air-drying pretreatment that tends to increase soil P solubility.In this study,the effects of air-drying on the distribution of Hedley P fractions were examined using soils collected under 4 forest types at Gongga Mountain,southwestern China.The results showed that the microbial biomass P(Pmic)in the organic horizon decreased markedly after air-drying.The concentrations of Hedley labile P in the air-dried samples were 31%–73%more than those in the field-moist samples.Consequently,the air-drying-induced increments of Hedley labile P pools in the surface soil horizons were 0.8–3.8 times the annual plant P requirements.The organic horizon was more susceptible to the air-drying-induced increases in Hedley labile P than the mineral horizon,probably because of the stronger release of Pmicand disruption of soil organic matter.The quality of P,indexed by the ratio of Hedley labile P to slowly cycling P,shifted in favor of the Hedley labile fractions after air-drying,further revealing that air-drying changed the distribution of Hedley P pools in forest soils.These indicated that the effects of air-drying could not be ignored when interpreting the discrepancy between the P status of plants and the Hedley labile P pools in forest soils.To more efficiently evaluate the P status in forest soils via the Hedley fractionation procedure,the use of field-moist soils is recommended.