Five commonly-used reference genes： ACT （actin）, UBE （ubiquitin-conjugating enzyme）, RPL2 （ribosomal protein L2）, BRP II （RNA polymerase II subunit）, and NADH （nicotinamide adenine dinucleotide） were examin...Five commonly-used reference genes： ACT （actin）, UBE （ubiquitin-conjugating enzyme）, RPL2 （ribosomal protein L2）, BRP II （RNA polymerase II subunit）, and NADH （nicotinamide adenine dinucleotide） were examined using geNorm software as reference genes for RT-qPCR. Among the tested reference genes, ACT and UBE were the most stable in all samples. In parallel, expression analysis of nitrilases in Brassica juncea var. tumida, was performed to preliminarily investigate the molecular interactions between nitrilase and clubroot development at 10, 15, 20, 25, 30, and 40 d postinoculation （dpi） with a suspension of resting spores of Plasmodiophora brassicae. The results showed that different gene expressions of nitrilases were regulated during the initial periods of clubroot development. The expression level of BjNIT1 increased sharply from 20 to 40 dpi in infected roots while there were no remarkable changes in healthy roots. From 15 to 30 dpi, the expression levels of BjNIT2 and BjNIT4 in infected roots were lower than those in non-infected roots. Finally, BjNIT2 in treatment was down approximately to control at 40 dpi. Our results suggest that BjNIT1, which promoted overproductions of auxin, might be involved in P. brassicae infection of B. juncea.展开更多
以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的...以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的碱基差异;而与甘蓝链格孢和萝卜链格孢的碱基差异较明显,均存在大于3 bp的碱基差异,且存在大量的缺失片段。初步确定引起茎瘤芥黑斑病的病原菌为芸薹链格孢。展开更多
基金financially supported by the Natural Science Foundation of Chongqing Science and Technology Commission,China (2008BB1370)Fuling Agricultural Science Institute of Chongqing,China
文摘Five commonly-used reference genes： ACT （actin）, UBE （ubiquitin-conjugating enzyme）, RPL2 （ribosomal protein L2）, BRP II （RNA polymerase II subunit）, and NADH （nicotinamide adenine dinucleotide） were examined using geNorm software as reference genes for RT-qPCR. Among the tested reference genes, ACT and UBE were the most stable in all samples. In parallel, expression analysis of nitrilases in Brassica juncea var. tumida, was performed to preliminarily investigate the molecular interactions between nitrilase and clubroot development at 10, 15, 20, 25, 30, and 40 d postinoculation （dpi） with a suspension of resting spores of Plasmodiophora brassicae. The results showed that different gene expressions of nitrilases were regulated during the initial periods of clubroot development. The expression level of BjNIT1 increased sharply from 20 to 40 dpi in infected roots while there were no remarkable changes in healthy roots. From 15 to 30 dpi, the expression levels of BjNIT2 and BjNIT4 in infected roots were lower than those in non-infected roots. Finally, BjNIT2 in treatment was down approximately to control at 40 dpi. Our results suggest that BjNIT1, which promoted overproductions of auxin, might be involved in P. brassicae infection of B. juncea.
文摘以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的碱基差异;而与甘蓝链格孢和萝卜链格孢的碱基差异较明显,均存在大于3 bp的碱基差异,且存在大量的缺失片段。初步确定引起茎瘤芥黑斑病的病原菌为芸薹链格孢。
