The OVATE family proteins(OFPs) are plant-specific proteins that modulate diverse aspects of plant growth and development. In tomato, OFP20 has been shown to interact with TONNEAU1 Recruiting Motif(TRM) proteins to re...The OVATE family proteins(OFPs) are plant-specific proteins that modulate diverse aspects of plant growth and development. In tomato, OFP20 has been shown to interact with TONNEAU1 Recruiting Motif(TRM) proteins to regulate fruit shape. In this study, we demonstrated that the mutation of StOFP20 caused a shift from round to oval shaped tubers in a diploid accession C151, supporting the role of StOFP20 in controlling tuber shape. Its expression reached a maximum in the tuber initiation stage and then decreased as the tuber develops. To help elucidate the mechanism of tuber shape regulation by StOFP20, 27 TONNEAU1 Recruiting Motif(TRM) proteins were identified and 23 of them were successfully amplified in C151. A yeast two-hybrid assay identified three TRM proteins that interacted with StOFP20, which was confirmed by firefly luciferase complementation in tobacco leaves. The OVATE domain was indispensable for the interactions, while the necessity of the M10 motif in TRM proteins varied among the interactions between StOFP20 and the three TRMs. In summary, both StOFP20 and SlOFP20 directed interactions with TRM proteins, but the corresponding interactants were not completely consistent, implying that they exert regulatory roles through mechanisms that are only partially overlapping.展开更多
OFP(Ovate Family Protein)基因家族包含有众多基因,其中番茄中的SlOFP20调控番茄果实形状,马铃薯中的同源基因StOFP20调控块茎形状,但StOFP20的编码序列并不清楚.利用薯形分离的二倍体马铃薯自交群体,精细定位并克隆了StOFP20.StOFP20...OFP(Ovate Family Protein)基因家族包含有众多基因,其中番茄中的SlOFP20调控番茄果实形状,马铃薯中的同源基因StOFP20调控块茎形状,但StOFP20的编码序列并不清楚.利用薯形分离的二倍体马铃薯自交群体,精细定位并克隆了StOFP20.StOFP20基因全长为1041 bp,在圆形薯块中能够扩增出来,在椭圆形薯块中缺失.展开更多
基金supported by the National Natural Science Foundation of China(32060684)the Academician Workstation of Yunnan,China(202105AF150028)。
文摘The OVATE family proteins(OFPs) are plant-specific proteins that modulate diverse aspects of plant growth and development. In tomato, OFP20 has been shown to interact with TONNEAU1 Recruiting Motif(TRM) proteins to regulate fruit shape. In this study, we demonstrated that the mutation of StOFP20 caused a shift from round to oval shaped tubers in a diploid accession C151, supporting the role of StOFP20 in controlling tuber shape. Its expression reached a maximum in the tuber initiation stage and then decreased as the tuber develops. To help elucidate the mechanism of tuber shape regulation by StOFP20, 27 TONNEAU1 Recruiting Motif(TRM) proteins were identified and 23 of them were successfully amplified in C151. A yeast two-hybrid assay identified three TRM proteins that interacted with StOFP20, which was confirmed by firefly luciferase complementation in tobacco leaves. The OVATE domain was indispensable for the interactions, while the necessity of the M10 motif in TRM proteins varied among the interactions between StOFP20 and the three TRMs. In summary, both StOFP20 and SlOFP20 directed interactions with TRM proteins, but the corresponding interactants were not completely consistent, implying that they exert regulatory roles through mechanisms that are only partially overlapping.
文摘OFP(Ovate Family Protein)基因家族包含有众多基因,其中番茄中的SlOFP20调控番茄果实形状,马铃薯中的同源基因StOFP20调控块茎形状,但StOFP20的编码序列并不清楚.利用薯形分离的二倍体马铃薯自交群体,精细定位并克隆了StOFP20.StOFP20基因全长为1041 bp,在圆形薯块中能够扩增出来,在椭圆形薯块中缺失.
