Objective: The present study is to investigate the expression of CD34, β-Tubulin-III and Collagen IV-Laminin in adenocarcinoma in situ (AIS), the AIS component of minimally invasive adenocarcinoma (MIA), and early in...Objective: The present study is to investigate the expression of CD34, β-Tubulin-III and Collagen IV-Laminin in adenocarcinoma in situ (AIS), the AIS component of minimally invasive adenocarcinoma (MIA), and early invasive foci, in order to find a valuable immunohistochemical marker for discriminating AIS and its early invasive foci. Methods: A total of 51 AIS patients and 88 MIA patients were included in the present study. In addition, 40 atypical adenomatous hyperplasia (AAH) patients and 54 invasive adenocarcinoma (IA) patients were included as control. Immunohisto-chemical staining of β-Tubulin-III, CD34, CD31, F8 and Collagen IV-Laminin was performed by serial sectioning. β-Tubulin-III was used to show invasive adenocarcinoma foci, CD34 was used to indicate interstitial cells in AIS, CD31 and F8 were used to identify capillary endothelial cells in tumor tissues, and Collagen IV-Laminin was used to visualize the basement membrane component of AIS. Results: The basement membranes and interstitial cells of AAH, AIS and the AIS component of MIA had positive expression of CD34, while mucinous AIS and various invasive adenocarcinomas had no CD34-positive basement membranes or interstitial cells. Invasive cancers such as alveolar adenocarcinoma, papillary adenocarcinoma, micropapillary adenocarcinoma and solid adenocarcinoma had strong positive expression of β-Tubulin-III, while AAH, AIS and the AIS component of MIA, and invasive mucinous adenocarcinoma had negative expression of β-Tubulin-III. AAH, AIS and the AIS component of MIA were surrounded by basement membranes with positive expression of Collagen IV-Laminin, AIS and the AIS component of MIA had significantly thickened basement membranes, and none of invasive adenocarcinomas was surrounded by basement membranes. Conclusions: The present study demonstrates that immunohistochemical staining of CD34, β-Tubulin-III, and Collagen IV-Laminin discriminates AIS component of lung adenocarcinoma from early invasive foci, with the efficacy of β-Tubulin-III being the best. Staining of β-Tubulin-III precisely identifies the early invasive foci of MIA, and can be used as a marker for the identification of the early invasive foci of nonmucinous lung adenocarcinoma.展开更多
【目的】通过分析棉花枯萎病菌的遗传多样性,探究新疆棉花枯萎病菌株的分群及其演化。【方法】2022年在新疆不同植棉区共分离出22株棉花枯萎病菌株,对延伸因子1α(elongation factor-1α,EF-1α)和β微管蛋白基因进行扩增、测序,并从美...【目的】通过分析棉花枯萎病菌的遗传多样性,探究新疆棉花枯萎病菌株的分群及其演化。【方法】2022年在新疆不同植棉区共分离出22株棉花枯萎病菌株,对延伸因子1α(elongation factor-1α,EF-1α)和β微管蛋白基因进行扩增、测序,并从美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库获取36个棉花枯萎病菌株的相关基因序列信息。基于上述基因序列分别进行系统进化分析和单倍型分析。【结果】基于57条EF-1α基因序列的进化树分析表明,棉花枯萎病菌可分为3大群,第1大群包含来自新疆、河北和澳大利亚的共31个枯萎病菌株,该大群可分成4个亚群;第2大群包含25个枯萎病菌株,构成比较复杂,可分成3个亚群;第3大群仅包含美国菌株LA140。基于28条β微管蛋白基因序列的进化树分析表明,本次分离的新疆棉花枯萎病菌株与棉花枯萎病菌7号和8号生理小种不同。根据EF-1α基因序列构建的单倍型网络将棉花枯萎病菌株分为19个单倍型,新疆21个棉花枯萎病菌株归属于有共同起源的5种单倍型。【结论】本研究分离的新疆棉花枯萎病菌株与已报道的棉花枯萎病菌1~8号生理小种均不相同,但与河北菌株的亲缘关系较近。EF-1α单倍型分析表明,本研究中的所有棉花枯萎病菌均从1号生理小种演化而来。展开更多
文摘Objective: The present study is to investigate the expression of CD34, β-Tubulin-III and Collagen IV-Laminin in adenocarcinoma in situ (AIS), the AIS component of minimally invasive adenocarcinoma (MIA), and early invasive foci, in order to find a valuable immunohistochemical marker for discriminating AIS and its early invasive foci. Methods: A total of 51 AIS patients and 88 MIA patients were included in the present study. In addition, 40 atypical adenomatous hyperplasia (AAH) patients and 54 invasive adenocarcinoma (IA) patients were included as control. Immunohisto-chemical staining of β-Tubulin-III, CD34, CD31, F8 and Collagen IV-Laminin was performed by serial sectioning. β-Tubulin-III was used to show invasive adenocarcinoma foci, CD34 was used to indicate interstitial cells in AIS, CD31 and F8 were used to identify capillary endothelial cells in tumor tissues, and Collagen IV-Laminin was used to visualize the basement membrane component of AIS. Results: The basement membranes and interstitial cells of AAH, AIS and the AIS component of MIA had positive expression of CD34, while mucinous AIS and various invasive adenocarcinomas had no CD34-positive basement membranes or interstitial cells. Invasive cancers such as alveolar adenocarcinoma, papillary adenocarcinoma, micropapillary adenocarcinoma and solid adenocarcinoma had strong positive expression of β-Tubulin-III, while AAH, AIS and the AIS component of MIA, and invasive mucinous adenocarcinoma had negative expression of β-Tubulin-III. AAH, AIS and the AIS component of MIA were surrounded by basement membranes with positive expression of Collagen IV-Laminin, AIS and the AIS component of MIA had significantly thickened basement membranes, and none of invasive adenocarcinomas was surrounded by basement membranes. Conclusions: The present study demonstrates that immunohistochemical staining of CD34, β-Tubulin-III, and Collagen IV-Laminin discriminates AIS component of lung adenocarcinoma from early invasive foci, with the efficacy of β-Tubulin-III being the best. Staining of β-Tubulin-III precisely identifies the early invasive foci of MIA, and can be used as a marker for the identification of the early invasive foci of nonmucinous lung adenocarcinoma.
文摘【目的】通过分析棉花枯萎病菌的遗传多样性,探究新疆棉花枯萎病菌株的分群及其演化。【方法】2022年在新疆不同植棉区共分离出22株棉花枯萎病菌株,对延伸因子1α(elongation factor-1α,EF-1α)和β微管蛋白基因进行扩增、测序,并从美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库获取36个棉花枯萎病菌株的相关基因序列信息。基于上述基因序列分别进行系统进化分析和单倍型分析。【结果】基于57条EF-1α基因序列的进化树分析表明,棉花枯萎病菌可分为3大群,第1大群包含来自新疆、河北和澳大利亚的共31个枯萎病菌株,该大群可分成4个亚群;第2大群包含25个枯萎病菌株,构成比较复杂,可分成3个亚群;第3大群仅包含美国菌株LA140。基于28条β微管蛋白基因序列的进化树分析表明,本次分离的新疆棉花枯萎病菌株与棉花枯萎病菌7号和8号生理小种不同。根据EF-1α基因序列构建的单倍型网络将棉花枯萎病菌株分为19个单倍型,新疆21个棉花枯萎病菌株归属于有共同起源的5种单倍型。【结论】本研究分离的新疆棉花枯萎病菌株与已报道的棉花枯萎病菌1~8号生理小种均不相同,但与河北菌株的亲缘关系较近。EF-1α单倍型分析表明,本研究中的所有棉花枯萎病菌均从1号生理小种演化而来。
