In worldwide,the use of chemical pesticides to protect wood has been greatly restricted.In recent years,a large number of researchers devoted to the search for natural,safe and non-polluting bioactive chemical compoun...In worldwide,the use of chemical pesticides to protect wood has been greatly restricted.In recent years,a large number of researchers devoted to the search for natural,safe and non-polluting bioactive chemical compounds from plants as an alternative to synthetic organic chemical preservative.In Chinese folk,tung oil can be used as paint for wooden furniture to protect them from pests.This study aimed to evaluate the chemical compositions of raw and heated tung oil and their activity against termite.In choice bioassays,weight loss of wood treated with 5%raw or heated tung oil after 4 weeks was significantly less than that of the control group.In no-choice bioassays,there was a significant difference in termite survival and wood weight loss on raw and heated tung oil-treated wood.When tung oil-treatment concentrations increased to 5%,wood weight loss was less than 10%.There was no significant difference in termite survival and wood weight loss between raw and heated tung oil-treated wood.Survival of termites in both tung oil wood treatments was significantly lower than that in the starvation control after 4 weeks.Raw and heated tung oil significantly improved the resistance of pine wood to termites,and have the potential for the development of natural wood preservatives.展开更多
Many metabolites in leaf tissue disturbed plant genomic DNA isolation and always varied when leaves was harvested from different environments. Objective of this study was to investigate whether season, environment str...Many metabolites in leaf tissue disturbed plant genomic DNA isolation and always varied when leaves was harvested from different environments. Objective of this study was to investigate whether season, environment stress and refrigerated storage affect genomic DNA isolation of tung tree leaves. Five types of young leaves and two DNA isolation protocols, the recycling CTAB protocol I and II, were adopted to carry out the experiment. Our results showed that both leaf type and protocol affected DNA isolation of tung tree. Using the recycling CTAB protocol II, though little DNA were obtained from three types of young leaves, the other two have satisfying results. Whereas the recycling CTAB protocol I could produce high yield genomic DNA from all the five types of young leaves. All the detectable DNA samples in agarose gel electrophoresis were good templates for PCR reaction. Season, environment stress and refrigerated storage had a big effect on genomic DNA isolation of tung tree. The recycling CTAB protocol I was proved to be an effective and universal protocol for DNA isolation of tung tree. Five types of young leaves could all act as the tissue for isolation of genomic DNA, but the summer healthy young leaves without long-time refrigerated storage are the best. The optimal leaf tissue will benefit DNA isolation of plant species.展开更多
Tung tree (Vernicia fordii) is an economically important woody oil plant that produces tung oil composed of approximately 80%a-eleostearic acid,an unusual fatty acid.Tung oil has been used as a drying ingredient in pa...Tung tree (Vernicia fordii) is an economically important woody oil plant that produces tung oil composed of approximately 80%a-eleostearic acid,an unusual fatty acid.Tung oil has been used as a drying ingredient in paints,varnishes,and coatings for more than 2000 years.For better conservation and utilization of this species,the Central South University of Forestry and Technology.展开更多
油桐是我国重要的木本油料植物,过去对油桐的研究主要集中于栽培和常规育种,与油桐种仁油脂合成相关的分子机理研究还未见报道。本研究在油桐种仁油脂合成期的转录组数据基础上,通过生物信息学方法,将不饱和脂肪酸生物合成途径中的45条U...油桐是我国重要的木本油料植物,过去对油桐的研究主要集中于栽培和常规育种,与油桐种仁油脂合成相关的分子机理研究还未见报道。本研究在油桐种仁油脂合成期的转录组数据基础上,通过生物信息学方法,将不饱和脂肪酸生物合成途径中的45条Unigene序列在KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库中进行比对,获得了12个关键酶的同源蛋白质并对编码这些同源蛋白质的基因在油桐种仁油脂合成期的表达模式进行了分析。通过数据分析,本研究初步推导了油桐种仁不饱和脂肪酸合成途径,这将为油桐油脂合成,尤其是α-桐酸形成机理的解析奠定基础,并为进一步的理论研究和油桐的遗传改良提供潜在的基因资源。展开更多
基金This study was funded by College Students’Scientific Research Training Program of Zhejiang A&F University(No.113-2013200148)ZAFU-Institute of Termite Control of Yuhang cooperative project(2045200485,2045200529).
文摘In worldwide,the use of chemical pesticides to protect wood has been greatly restricted.In recent years,a large number of researchers devoted to the search for natural,safe and non-polluting bioactive chemical compounds from plants as an alternative to synthetic organic chemical preservative.In Chinese folk,tung oil can be used as paint for wooden furniture to protect them from pests.This study aimed to evaluate the chemical compositions of raw and heated tung oil and their activity against termite.In choice bioassays,weight loss of wood treated with 5%raw or heated tung oil after 4 weeks was significantly less than that of the control group.In no-choice bioassays,there was a significant difference in termite survival and wood weight loss on raw and heated tung oil-treated wood.When tung oil-treatment concentrations increased to 5%,wood weight loss was less than 10%.There was no significant difference in termite survival and wood weight loss between raw and heated tung oil-treated wood.Survival of termites in both tung oil wood treatments was significantly lower than that in the starvation control after 4 weeks.Raw and heated tung oil significantly improved the resistance of pine wood to termites,and have the potential for the development of natural wood preservatives.
文摘Many metabolites in leaf tissue disturbed plant genomic DNA isolation and always varied when leaves was harvested from different environments. Objective of this study was to investigate whether season, environment stress and refrigerated storage affect genomic DNA isolation of tung tree leaves. Five types of young leaves and two DNA isolation protocols, the recycling CTAB protocol I and II, were adopted to carry out the experiment. Our results showed that both leaf type and protocol affected DNA isolation of tung tree. Using the recycling CTAB protocol II, though little DNA were obtained from three types of young leaves, the other two have satisfying results. Whereas the recycling CTAB protocol I could produce high yield genomic DNA from all the five types of young leaves. All the detectable DNA samples in agarose gel electrophoresis were good templates for PCR reaction. Season, environment stress and refrigerated storage had a big effect on genomic DNA isolation of tung tree. The recycling CTAB protocol I was proved to be an effective and universal protocol for DNA isolation of tung tree. Five types of young leaves could all act as the tissue for isolation of genomic DNA, but the summer healthy young leaves without long-time refrigerated storage are the best. The optimal leaf tissue will benefit DNA isolation of plant species.
文摘Tung tree (Vernicia fordii) is an economically important woody oil plant that produces tung oil composed of approximately 80%a-eleostearic acid,an unusual fatty acid.Tung oil has been used as a drying ingredient in paints,varnishes,and coatings for more than 2000 years.For better conservation and utilization of this species,the Central South University of Forestry and Technology.
文摘油桐是我国重要的木本油料植物,过去对油桐的研究主要集中于栽培和常规育种,与油桐种仁油脂合成相关的分子机理研究还未见报道。本研究在油桐种仁油脂合成期的转录组数据基础上,通过生物信息学方法,将不饱和脂肪酸生物合成途径中的45条Unigene序列在KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库中进行比对,获得了12个关键酶的同源蛋白质并对编码这些同源蛋白质的基因在油桐种仁油脂合成期的表达模式进行了分析。通过数据分析,本研究初步推导了油桐种仁不饱和脂肪酸合成途径,这将为油桐油脂合成,尤其是α-桐酸形成机理的解析奠定基础,并为进一步的理论研究和油桐的遗传改良提供潜在的基因资源。
