BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unkn...BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unknown.AIM To explore potential molecular connections between H.pylori infection and T2DM.METHODS We extracted gene expression arrays from three online datasets(GSE60427,GSE27411 and GSE115601).Differentially expressed genes(DEGs)commonly present in patients with H.pylori infection and T2DM were identified.Hub genes were validated using human gastric biopsy samples.Correlations between hub genes and immune cell infiltration,miRNAs,and transcription factors(TFs)were further analyzed.RESULTS A total of 67 DEGs were commonly presented in patients with H.pylori infection and T2DM.Five significantly upregulated hub genes,including TLR4,ITGAM,C5AR1,FCER1G,and FCGR2A,were finally identified,all of which are closely related to immune cell infiltration.The gene-miRNA analysis detected 13 miRNAs with at least two gene cross-links.TF-gene interaction networks showed that TLR4 was coregulated by 26 TFs,the largest number of TFs among the 5 hub genes.CONCLUSION We identified five hub genes that may have molecular connections between H.pylori infection and T2DM.This study provides new insights into the pathogenesis of H.pylori-induced onset of T2DM.展开更多
BACKGROUND The identification of specific gene expression patterns is crucial for understanding the mechanisms underlying primary biliary cholangitis(PBC)and finding relevant biomarkers for diagnosis and therapeutic e...BACKGROUND The identification of specific gene expression patterns is crucial for understanding the mechanisms underlying primary biliary cholangitis(PBC)and finding relevant biomarkers for diagnosis and therapeutic evaluation.AIM To determine PBC-associated hub genes and assess their clinical utility for disease prediction.METHODS PBC expression data were obtained from the Gene Expression Omnibus database.Overlapping genes from differential expression analysis and weighted gene coexpression network analysis(WGCNA)were identified as key genes for PBC.Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses were performed to explore the potential roles of key genes.Hub genes were identified in protein-protein interaction(PPI)networks using the Degree algorithm in Cytoscape software.The relationship between hub genes and immune cells was investigated.Finally,a Mendelian randomization study was conducted to determine the causal effects of hub genes on PBC.RESULTS We identified 71 overlapping key genes using differential expression analysis and WGCNA.These genes were primarily enriched in pathways related to cytokinecytokine receptor interaction,and Th1,Th2,and Th17 cell differentiation.We utilized Cytoscape software and identified five hub genes(CD247,IL10,CCL5,CCL3,and STAT3)in PPI networks.These hub genes showed a strong correlation with immune cell infiltration in PBC.However,inverse variance weighting analysis did not indicate the causal effects of hub genes on PBC risk.CONCLUSION Hub genes can potentially serve as valuable biomarkers for PBC prediction and treatment,thereby offering significant clinical utility.展开更多
The target of integrated construction of comprehensive transportation hub is to integrate the traffic resources,achieve butt joint of pan-regional transportation mode,and finally realize the"seamless connection&q...The target of integrated construction of comprehensive transportation hub is to integrate the traffic resources,achieve butt joint of pan-regional transportation mode,and finally realize the"seamless connection"of the goods and"zero transfer"of the passenger traffic.Relying on the particularity of the geographical location and the convenience of the Yangtze River channel,Luzhou puts forth effort to build a comprehensive transport hub in Southern Sichuan and has made great efforts in traffic infrastructure construction.However,there are still some problems.Combining practice of Luzhou,using the advanced experience of foreign and domestic cities for reference,the paper pointed out that for the sake of constructing a comprehensive transportation hub,we need to build the traffic integration on the basis of the efficiency.Besides,the paper proposed the strategies for construction.展开更多
The external rotor hub motor adopts direct drive mode,no deceleration drive device,and has a compact structure.Its axial size is smaller than that of a deceleration-driven hub motor,which greatly reduces the weight of...The external rotor hub motor adopts direct drive mode,no deceleration drive device,and has a compact structure.Its axial size is smaller than that of a deceleration-driven hub motor,which greatly reduces the weight of the vehicle and increases the cruising range of the vehicle.Because of the limited special working environment and performance requirements,the hub motor has a small internal space and a large heat generation,so it puts forward higher requirements for heat dissipation capacity.For the external rotor hub motor,a new type of in-tank watercooled structure of hub motor was proposed to improve its cooling effect and performance.Firstly,the threedimensional finite element model of the motor is established to analyze the characteristics of motor loss and temperature field distribution.Secondly,the cooling performance of different cooling structures in the tank was studied.Finally,the thermal network model and three-dimensional finite element analysis were used to optimize the water-cooled structure in the tank,and the power density of themotor was improved by improving the cooling performance under the condition of volume limitation of the hub motor.The results show that the cooling effect of the proposed water-cooled structure in the tank is significant under the condition of constant power density.Compared to natural ventilation,the maximum temperature was reduced by 33.13°C and the cooling effect was increased by about 27.7%.展开更多
This article discusses the roadbed splicing for hub interchanges.The article starts with a description of the characteristics of junction roadbed splicing.The application of splicing technology is explained using a su...This article discusses the roadbed splicing for hub interchanges.The article starts with a description of the characteristics of junction roadbed splicing.The application of splicing technology is explained using a subgrade splicing scheme of a project.Roadbed splicing involves stepwise excavation and preparative measures like surface cleaning and backfilling.This article serves to provide a valuable reference for road and bridge construction and improve the quality of China’s road and bridge projects,so as to achieve sustainable development of the road and bridge engineering industry.展开更多
基金Supported by National Natural Science Foundation of China,No.82100594.
文摘BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unknown.AIM To explore potential molecular connections between H.pylori infection and T2DM.METHODS We extracted gene expression arrays from three online datasets(GSE60427,GSE27411 and GSE115601).Differentially expressed genes(DEGs)commonly present in patients with H.pylori infection and T2DM were identified.Hub genes were validated using human gastric biopsy samples.Correlations between hub genes and immune cell infiltration,miRNAs,and transcription factors(TFs)were further analyzed.RESULTS A total of 67 DEGs were commonly presented in patients with H.pylori infection and T2DM.Five significantly upregulated hub genes,including TLR4,ITGAM,C5AR1,FCER1G,and FCGR2A,were finally identified,all of which are closely related to immune cell infiltration.The gene-miRNA analysis detected 13 miRNAs with at least two gene cross-links.TF-gene interaction networks showed that TLR4 was coregulated by 26 TFs,the largest number of TFs among the 5 hub genes.CONCLUSION We identified five hub genes that may have molecular connections between H.pylori infection and T2DM.This study provides new insights into the pathogenesis of H.pylori-induced onset of T2DM.
基金Supported by School-Level Key Projects at Bengbu Medical College,No.2021byzd109。
文摘BACKGROUND The identification of specific gene expression patterns is crucial for understanding the mechanisms underlying primary biliary cholangitis(PBC)and finding relevant biomarkers for diagnosis and therapeutic evaluation.AIM To determine PBC-associated hub genes and assess their clinical utility for disease prediction.METHODS PBC expression data were obtained from the Gene Expression Omnibus database.Overlapping genes from differential expression analysis and weighted gene coexpression network analysis(WGCNA)were identified as key genes for PBC.Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses were performed to explore the potential roles of key genes.Hub genes were identified in protein-protein interaction(PPI)networks using the Degree algorithm in Cytoscape software.The relationship between hub genes and immune cells was investigated.Finally,a Mendelian randomization study was conducted to determine the causal effects of hub genes on PBC.RESULTS We identified 71 overlapping key genes using differential expression analysis and WGCNA.These genes were primarily enriched in pathways related to cytokinecytokine receptor interaction,and Th1,Th2,and Th17 cell differentiation.We utilized Cytoscape software and identified five hub genes(CD247,IL10,CCL5,CCL3,and STAT3)in PPI networks.These hub genes showed a strong correlation with immune cell infiltration in PBC.However,inverse variance weighting analysis did not indicate the causal effects of hub genes on PBC risk.CONCLUSION Hub genes can potentially serve as valuable biomarkers for PBC prediction and treatment,thereby offering significant clinical utility.
文摘The target of integrated construction of comprehensive transportation hub is to integrate the traffic resources,achieve butt joint of pan-regional transportation mode,and finally realize the"seamless connection"of the goods and"zero transfer"of the passenger traffic.Relying on the particularity of the geographical location and the convenience of the Yangtze River channel,Luzhou puts forth effort to build a comprehensive transport hub in Southern Sichuan and has made great efforts in traffic infrastructure construction.However,there are still some problems.Combining practice of Luzhou,using the advanced experience of foreign and domestic cities for reference,the paper pointed out that for the sake of constructing a comprehensive transportation hub,we need to build the traffic integration on the basis of the efficiency.Besides,the paper proposed the strategies for construction.
基金supported by National Science Foundation of China(Grant No.51705306).
文摘The external rotor hub motor adopts direct drive mode,no deceleration drive device,and has a compact structure.Its axial size is smaller than that of a deceleration-driven hub motor,which greatly reduces the weight of the vehicle and increases the cruising range of the vehicle.Because of the limited special working environment and performance requirements,the hub motor has a small internal space and a large heat generation,so it puts forward higher requirements for heat dissipation capacity.For the external rotor hub motor,a new type of in-tank watercooled structure of hub motor was proposed to improve its cooling effect and performance.Firstly,the threedimensional finite element model of the motor is established to analyze the characteristics of motor loss and temperature field distribution.Secondly,the cooling performance of different cooling structures in the tank was studied.Finally,the thermal network model and three-dimensional finite element analysis were used to optimize the water-cooled structure in the tank,and the power density of themotor was improved by improving the cooling performance under the condition of volume limitation of the hub motor.The results show that the cooling effect of the proposed water-cooled structure in the tank is significant under the condition of constant power density.Compared to natural ventilation,the maximum temperature was reduced by 33.13°C and the cooling effect was increased by about 27.7%.
文摘This article discusses the roadbed splicing for hub interchanges.The article starts with a description of the characteristics of junction roadbed splicing.The application of splicing technology is explained using a subgrade splicing scheme of a project.Roadbed splicing involves stepwise excavation and preparative measures like surface cleaning and backfilling.This article serves to provide a valuable reference for road and bridge construction and improve the quality of China’s road and bridge projects,so as to achieve sustainable development of the road and bridge engineering industry.
