Besides excellent biodegradability and biocompatibility,a useful tissue engineering scaffold should provide favorable surface properties,outstanding mechanical strength and controlled drug release property. In this pa...Besides excellent biodegradability and biocompatibility,a useful tissue engineering scaffold should provide favorable surface properties,outstanding mechanical strength and controlled drug release property. In this paper,a mild process to prepare porous tussah silk fibroin( TSF) scaffolds from aqueous solution was described. The n-butanol was used to control the self-assembly of tussah silk. The scaffolds with different TSF concentrations and the same volume showed differences in pore size and distribution. The maximum porosity of the poprepared porous scaffolds was 80% in this paper. And the pore size of the prepared porous scaffolds with different concentrations was between 10μm and 230 μm. X-ray diffraction( XRD) analysis revealed that amorphous TSF was crystallized to β-sheet secondary structure upon gelatin. The TSF scaffolds for controlled drug release was studied and the result showed that the time of drug release was significantly longer. The produced TSF scaffolds with sustained drug release have potential application in tissue engineering.展开更多
该文探究柞蚕丝素蛋白的营养价值。采用化学和生物学方法测定并分析柞蚕丝素蛋白的5项消化指标(表观消化率、真消化率、蛋白质功效比值、生物价和蛋白质净利用率)以及对大鼠体内器官的相关影响。用柞蚕丝素蛋白饲料进行喂养实验,采用美...该文探究柞蚕丝素蛋白的营养价值。采用化学和生物学方法测定并分析柞蚕丝素蛋白的5项消化指标(表观消化率、真消化率、蛋白质功效比值、生物价和蛋白质净利用率)以及对大鼠体内器官的相关影响。用柞蚕丝素蛋白饲料进行喂养实验,采用美国官方分析化学师协会(Association of Official Analytical Chemists,AOAC)的方法进行给予饲料。把25只雄性SD大鼠放入代谢笼里进行单笼饲养,5只为一个实验组。饲养一段时间后,采集尿液和粪便进行定氮,计算相关指标。结果表明,高蛋白组真消化率(true digestibility,TD)和表观消化率(apparent digestibility,AD)分别为(54.65±4.00)%和(35.83±2.60)%、生物价(biological value,BV)为98.13±1.74、蛋白质净利用率(net protein utilization,NPU)为(56.67±2.93)%、蛋白质功效比值(protein efficiency ratio,PER)为-0.99±0.43;低蛋白组TD和AD分别为(57.77±5.08)%和(33.36±1.19)%、BV为96.35±2.66、NPU为(60.40±5.65)%、PER为(-0.99±0.43)。结果表明,高蛋白组表观消化率偏低、真消化率偏低,功效比值为负值。28 d喂养实验结束后,对大鼠进行解剖观察,发现大鼠主要器官无病变,血清指标均在正常范围之内,推测柞蚕丝素蛋白可以保存相应肌肉和能量,减少部分多余脂肪量。展开更多
基金Collaborative Innovation Center of Textile and Garment Industry of Henan Province,China(No.hnfz14004)
文摘Besides excellent biodegradability and biocompatibility,a useful tissue engineering scaffold should provide favorable surface properties,outstanding mechanical strength and controlled drug release property. In this paper,a mild process to prepare porous tussah silk fibroin( TSF) scaffolds from aqueous solution was described. The n-butanol was used to control the self-assembly of tussah silk. The scaffolds with different TSF concentrations and the same volume showed differences in pore size and distribution. The maximum porosity of the poprepared porous scaffolds was 80% in this paper. And the pore size of the prepared porous scaffolds with different concentrations was between 10μm and 230 μm. X-ray diffraction( XRD) analysis revealed that amorphous TSF was crystallized to β-sheet secondary structure upon gelatin. The TSF scaffolds for controlled drug release was studied and the result showed that the time of drug release was significantly longer. The produced TSF scaffolds with sustained drug release have potential application in tissue engineering.
文摘该文探究柞蚕丝素蛋白的营养价值。采用化学和生物学方法测定并分析柞蚕丝素蛋白的5项消化指标(表观消化率、真消化率、蛋白质功效比值、生物价和蛋白质净利用率)以及对大鼠体内器官的相关影响。用柞蚕丝素蛋白饲料进行喂养实验,采用美国官方分析化学师协会(Association of Official Analytical Chemists,AOAC)的方法进行给予饲料。把25只雄性SD大鼠放入代谢笼里进行单笼饲养,5只为一个实验组。饲养一段时间后,采集尿液和粪便进行定氮,计算相关指标。结果表明,高蛋白组真消化率(true digestibility,TD)和表观消化率(apparent digestibility,AD)分别为(54.65±4.00)%和(35.83±2.60)%、生物价(biological value,BV)为98.13±1.74、蛋白质净利用率(net protein utilization,NPU)为(56.67±2.93)%、蛋白质功效比值(protein efficiency ratio,PER)为-0.99±0.43;低蛋白组TD和AD分别为(57.77±5.08)%和(33.36±1.19)%、BV为96.35±2.66、NPU为(60.40±5.65)%、PER为(-0.99±0.43)。结果表明,高蛋白组表观消化率偏低、真消化率偏低,功效比值为负值。28 d喂养实验结束后,对大鼠进行解剖观察,发现大鼠主要器官无病变,血清指标均在正常范围之内,推测柞蚕丝素蛋白可以保存相应肌肉和能量,减少部分多余脂肪量。
