To observe the effects of different acupuncture manipulations on blood pressure and target organ damage in spontaneously hypertensive rats（SHRs）, this study used the reinforcing twirling method（1.5–2-mm depth; rot...To observe the effects of different acupuncture manipulations on blood pressure and target organ damage in spontaneously hypertensive rats（SHRs）, this study used the reinforcing twirling method（1.5–2-mm depth; rotating needle clockwise for 360° and then counter clockwise for 360°, with the thumb moving heavily forward and gently backward, 60 times per minute for 1 minute, and retaining needle for 9 minutes）, the reducing twirling method（1.5–2-mm depth; rotating needle counter clockwise for 360° and then clockwise for 360°, with the thumb moving heavily backward and gently forward, 60 times per minute for 1 minute, and retaining needle for 9 minutes）, and the needle retaining method（1.5–2-mm depth and retaining the needle for 10 minutes）. Bilateral Taichong（LR3） was treated by acupuncture using different manipulations and manual stimulation. Reinforcing twirling, reducing twirling, and needle retaining resulted in a decreased number of apoptotic cells, reduced Bax m RNA and protein expression, and an increased Bcl-2/Bax ratio in the hippocampus compared with the SHR group. Among these groups, the Bcl-2/Bax protein ratio was highest in the reducing twirling group, and the Bcl-2/Bax m RNA ratio was highest in the needle retaining group. These results suggest that reinforcing twirling, reducing twirling, and needle retaining methods all improve blood pressure and prevent target organ damage by increasing the hippocampal Bcl-2/Bax ratio and inhibiting cell apoptosis in the hippocampus in SHR.展开更多
OBJECTIVE: To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation(TRRM) might be the central mechanism underlying the action.METHODS: In the study,^(18) F-2-...OBJECTIVE: To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation(TRRM) might be the central mechanism underlying the action.METHODS: In the study,^(18) F-2-fluoro-deoxy-D-glucose positron emission tomography(18 F-FDG-PET)was employed. Fifity-six spontaneous hypertensive rats(SHRs) were randomly divided into a model group, a single-needle acupuncture(SNA) group, a twirling reinforcing group(SNA + TRF) and a twirling reducing(SNA + TRD) group. Fourteen Wistar rats were assigned to the control group. The acupuncture intervention at Taichong(LR 3) acupoint was administered once daily in the SNA, SNA + TRF and SNA + TRD groups for 14 days, with 1 d interval between the two weeks. The blood pressure(BP) of all rats was measured repeatedly and18 F-FDG-PET scans were conducted on the 14 th day. PET images were processed with Statistical Parametric Mapping 8.0.RESULTS: After the intervention, systolic BP showed a significant decrease in the SNA, SNA+TRF and SNA + TRD versus the model groups(all P <0.01) and in the SNA + TRF and SNA + TRD versus the SNA groups(both P < 0.01), with the SNA+TRD group exhibited the best antihypertensive effect(P < 0.01). The key brain regions activated by TRRM were mainly concentrated in the cerebellum, hippocampus, hypothalamus, medulla oblongata, insular cortex, midbrain, thalamus and visual cortex.CONCLUTION: TRRM could significantly lower the BP of SHRs by improving the cerebral glucose metabolism of the activated key brain regions and the underlying central mechanism may be related to the central rennin-angiotensin system and neurotransmission.展开更多
Objective To observe the clinical efficacy of acupuncture with twirling reducing method for tinnitus of excessive liver-fire type.Methods One hundred and one patients with tinnitus of excessive liver-fire type were ra...Objective To observe the clinical efficacy of acupuncture with twirling reducing method for tinnitus of excessive liver-fire type.Methods One hundred and one patients with tinnitus of excessive liver-fire type were randomly divided into a twirling reducing method group(35 cases),a needle retaining group(33 cases)and展开更多
基金supported by the National Natural Science Foundation of China,No.81072861,81373727
文摘To observe the effects of different acupuncture manipulations on blood pressure and target organ damage in spontaneously hypertensive rats（SHRs）, this study used the reinforcing twirling method（1.5–2-mm depth; rotating needle clockwise for 360° and then counter clockwise for 360°, with the thumb moving heavily forward and gently backward, 60 times per minute for 1 minute, and retaining needle for 9 minutes）, the reducing twirling method（1.5–2-mm depth; rotating needle counter clockwise for 360° and then clockwise for 360°, with the thumb moving heavily backward and gently forward, 60 times per minute for 1 minute, and retaining needle for 9 minutes）, and the needle retaining method（1.5–2-mm depth and retaining the needle for 10 minutes）. Bilateral Taichong（LR3） was treated by acupuncture using different manipulations and manual stimulation. Reinforcing twirling, reducing twirling, and needle retaining resulted in a decreased number of apoptotic cells, reduced Bax m RNA and protein expression, and an increased Bcl-2/Bax ratio in the hippocampus compared with the SHR group. Among these groups, the Bcl-2/Bax protein ratio was highest in the reducing twirling group, and the Bcl-2/Bax m RNA ratio was highest in the needle retaining group. These results suggest that reinforcing twirling, reducing twirling, and needle retaining methods all improve blood pressure and prevent target organ damage by increasing the hippocampal Bcl-2/Bax ratio and inhibiting cell apoptosis in the hippocampus in SHR.
基金Supported by the National Natural Science Foundation of China(Use PET Brain Imaging Technology to Study the Acupuncture Manipulation of Twirling Reinforcing-Reducing Regulation in Spontaneously Hypertensive Rats by Central Integration Signaling Network Construction,No.8157150840)
文摘OBJECTIVE: To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation(TRRM) might be the central mechanism underlying the action.METHODS: In the study,^(18) F-2-fluoro-deoxy-D-glucose positron emission tomography(18 F-FDG-PET)was employed. Fifity-six spontaneous hypertensive rats(SHRs) were randomly divided into a model group, a single-needle acupuncture(SNA) group, a twirling reinforcing group(SNA + TRF) and a twirling reducing(SNA + TRD) group. Fourteen Wistar rats were assigned to the control group. The acupuncture intervention at Taichong(LR 3) acupoint was administered once daily in the SNA, SNA + TRF and SNA + TRD groups for 14 days, with 1 d interval between the two weeks. The blood pressure(BP) of all rats was measured repeatedly and18 F-FDG-PET scans were conducted on the 14 th day. PET images were processed with Statistical Parametric Mapping 8.0.RESULTS: After the intervention, systolic BP showed a significant decrease in the SNA, SNA+TRF and SNA + TRD versus the model groups(all P <0.01) and in the SNA + TRF and SNA + TRD versus the SNA groups(both P < 0.01), with the SNA+TRD group exhibited the best antihypertensive effect(P < 0.01). The key brain regions activated by TRRM were mainly concentrated in the cerebellum, hippocampus, hypothalamus, medulla oblongata, insular cortex, midbrain, thalamus and visual cortex.CONCLUTION: TRRM could significantly lower the BP of SHRs by improving the cerebral glucose metabolism of the activated key brain regions and the underlying central mechanism may be related to the central rennin-angiotensin system and neurotransmission.
文摘Objective To observe the clinical efficacy of acupuncture with twirling reducing method for tinnitus of excessive liver-fire type.Methods One hundred and one patients with tinnitus of excessive liver-fire type were randomly divided into a twirling reducing method group(35 cases),a needle retaining group(33 cases)and
