The Pancharatnam Phase of a Three-Level Atom Coupled to Two Systems of N-Two Level Atoms

In this paper, we present the analytical solution for the model that describes the interaction between a three-level atom and two systems of N-two level atoms. The effects of the quantum numbers and the coupling parameters between spins on the Pancharatnam phase and the atomic inversion, for some special cases of the initial states, are investigated. The comparison between the two effects shows that the analytic results are well consistent.

Analysis of Protein Interactions:Probing the Function of Proteins with Yeast Two-Hybrid System

The yeast two\|hybrid system is a molecular genetic approach for protein interaction and it is widely used to screen for proteins that interact with a protein of interest in recent years.This process includes,construction and testing of the bait plasmid,screening a plasmid library for interacting fusion protein,elimination of false positives and delection analysis of true positives.This procedure is designed to allow investigators to identify proteins and their encoding cDNAs that have a biologically significant interaction with a protein of interest.More and more studies have demonstrated that the two\|hybrid system is a powerful and sensitive technique for the identification of genes that code for proteins that interact in a biologically significant fashion with a protein of interest in higher plants.This method has been used to identify new interaction protein in many laboratories.The recently reported yeast tri\|brid system,should allow the investigation of more complex protein\|protein interactions.The aim of this review is to outline the recent progress made in protein interactions by using yeast two\|hybrid system.

MEASUREMENT AND CORRELATION OF PARTITION COEFFICIENTS OF UROKINASE IN AQUEOUS TWO-PHASE PEG-POTASSIUM PHOSPHATE SYSTEM

Partition coefficients of Urokinase(UK)were measured in aqueous two-phase systems con-taining polyethylene glycol and potassium phosphate at 273.2K.Based on the Diamond-Hsu model,a modified expression was obtained for the correlation of enzyme partitioning in theabove-mentioned systems.Utilizing a modified form,the partitioning data of UK and heteroproteinwere correlated.The results show that the modified model is simple gives good precision.and will fa-cilitate engineering scale-up of aqueous two-phase systems for certain proteins purification.

Simultaneous phosphorus and nitrogen removal in a continuous-flow two-sludge system

The ability of simultaneous biological phosphorus and nitrogen removal was investigated in a lab-scale continuous-flow two-sludge system. Alternating anaerobic and anoxic conditions were combined with contact oxidation stage for treating raw municipal wastewater. Long-term experiments showed that the contradiction of competing for the organic substrate between denitrifying bacteria and PAOs （phosphorus accumulating organisms） in traditional phosphorus and nitrogen removal system has been resolved. The system can adapt to low influent COD/TN ratio （C/N）. Furthermore the SRT （sludge retention time） of nitrifying sludge and denitrifying phosphorus removal sludge can be controlled at optimal conditions respectively. The removal efficiency of COD, TP, TN, and NH4-N was 81.78%, 92.51%, 75.75%, and 84.47% respectively. It was also found that the appropriate influent C/N should be controlled at the range of 3.8-6, while the optimal C/N to the system ranged between 4-5, and the BFR （bypass sludge flow rate） should be controlled at 0.35 around.

The PhoR/PhoP two-component system regulates fengycin production in Bacillus subtilis NCD-2 under low-phosphate conditions

Bacillus subtilis strain NCD-2 is an excellent biocontrol agent for plant soil-borne diseases, and the lipopeptide fengycin is one of the active antifungal compounds in strain NCD-2. The regulator PhoP and its sensor kinase PhoR compose a two-component system in B. subtilis. In this study, the phoR- and phoP-knockout mutants were constructed by in-frame deletion and the role of PhoR/PhoP on the production of fengycin was determined. Inactivation of phoR or phoP in B. subtilis decreased its inhibition ability against Botrytis cinerea growth in vitro compared to the strain NCD-2 wild type. The lipopeptides were extracted from strain NCD-2 wild type and its mutant strains by hydrochloric acid precipitate, and the lipopeptides from phoR-null mutant orphoP-null mutant almost lost the inhibition ability against B. cinerea growth compared to the lipopeptides from strain NCD-2 wild type. Fast protein liquid chromatography （FPLC） analysis of the lipopeptides showed that inactivation of phoR or phoP genes reduced the production of fengycin by strain NCD-2. The fengycin production abilities were compared for bacteria under low-phosphate medium （LPM） and high-phosphate medium （HPM）, respectively. Results indicated that the regulation of fengycin production by the PhoR/PhoP two-component system occurred in LPM but not in HPM. Reverse transcriptionaI-PCR confirmed that the fengycin synthetase gene fenC was positively regulated by phoP when cultured in LPM. All of these characteristics could be partially restored by complementation of intact phoR or phoP gene in the mutant. These data indicated that the PhoR/PhoP two-component system greatly regulated fengycin production and antifungal ability in B. subtilis NCD-2 mainly under low-phosphate conditions.

Phosphorus accumulation by bacteria isolated from a continuous-flow two-sludge system

In this article, polyphosphate-accumulating organisms （PAOs） from a lab-scale continuous-flow two-sludge system was isolated and identified, the different phosphorus accumulation characteristics of the isolates under anoxic and aerobic conditions were investigated. Two kinds of PAOs were both found in the anoxic zones of the two-sludge system, one of them utilized only oxygen as electron aeceptor, and the other one utilized either nitrate or oxygen as electron aeceptor. Of the total eight isolates, five isolates were capable of utilizing both nitrate and oxygen as electron acceptors to uptake phosphorus to some extent. And three of the five isolates showed good phosphorus accumulative capacities both under anoxic or aerobic conditions, two identified as Alcaligenes and one identified as Pseudomonas. Streptococcus was observed weak anoxic phosphorus accumulation because of its weak denitrification capacity, but it showed good phosphorus accumulation capacity under aerobic conditions. One isolates identified as Enterobacteriaceae was proved to be a special species of PAOs, which could only uptake small amounts of phosphorus under anoxic conditions, although its denitrification capacity and aerobic phosphorus accumulation capacity were excellent.

High temperature magnetic semiconductors: narrow band gaps and two-dimensional systems

Magnetic semiconductors have been demonstrated to work at low temperatures, but not yet at room temperature for spin electronic applications. In contrast to the p-type diluted magnetic semiconductors, n-type diluted magnetic semiconductors are few. Using a combined method of the density function theory and quantum Monte Carlo simulation, we briefly discuss the recent progress to obtain diluted magnetic semiconductors with both p- and n-type carriers by choosing host semiconductors with a narrow band gap. In addition, the recent progress on two-dimensional intrinsic magnetic semiconductors with possible room temperature ferromangetism and quantum anomalous Hall effect are also discussed.

Maximal and total skew information for a two-qubit system using nonlinear interaction models

Maximal and total skew information is studied. For symmetric pure states of two-qubit, they are closely related to the linear entropy, the concurrence, and the spin squeezing parameter. For a two-qubit system implemented in three nonlinear interaction models with an external field, we give the exact state vectors and the expectation value （Sz） at any time t. Based on （Sz）2, we give the maximal and the total skew information and a condition in which the maximal and the total skew information can reach 1 and 2, respectively.

Measurement and Correlation of Equilibrium Data for Aqueous Two-phase System Ethanol+Water+ K_2HPO_4

The isothermal solubility data of aqueous two-phase system ethanol+water+K 2HPO 4 were determined with the turbidity titration method at 303.2 K. The binodal curves were described by using the Mistry equation very well. An experimental procedure for measuring the liquid-liquid equilibrium data of the aqueous two-phase system was proposed, in which the concentrations of the coexisting phases were determined with the corresponding densities of the solution. The tie lines were satisfactorily described by using the Othmer-Tobias and Bancroft equations.

Modified Two-Degrees-of-Freedom Internal Model Control for Non-Square Systems with Multiple Time Delays

A modified two-degrees-of-freedom( M-TDOF) internal model control( IMC) method is proposed for non-square systems with multiple time delays and right-half-plane( RHP) zeros. In this method,pseudo-inverse is introduced to design the internal model controller,and a desired closed-loop transfer function is designed to eliminate the unrealizable factors of the derived controller. In addition,set-point tracking and load-disturbance rejection of each process are separately controlled by two controllers. The simulation results show that in addition to high decoupling performance and robustness,the proposed control method also effectively improves loaddisturbance rejection and simultaneously optimizes the input tracking performance and disturbance rejection performance by selecting the parameters of controllers. Furthermore,the higher tolerance of model mismatch is achieved in this paper.

Ionic Liquid-salt Aqueous Two-phase System, a Novel System for theExtraction of Abused Drugs

A 1-butyl-3-methylimidazolium chloride-salt aqueous two-phase system was studied on extraction of abused drugs. The effects of sorts of salts, temperature, concentration of salt and drugs on system were investigated systematically. A satisfactory extraction efficiency of 93% was obtained for papaverine while that of morphine was 65%. The extraction mechanism was primarily discussed.

Generation of transient chaos from two-dimensional systems via a switching approach

A new approach of generating transient chaos from two-dimensional（2D） continuous autonomous systems within finite time is presented.Based on an absolute-value switching law,the phenomenon of transient chaos takes place by switching between three 2D systems.Basic dynamic behavior of the systems is investigated.Numerical examples illustrate the validity of the results.

Screening of hepatocyte proteins binding to NS5ABP37 protein by yeast-two hybrid system

Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in hepatocytes.Methods We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus(HCV)by cloning the gene of NS5ABP37 protein into pGBKT7,then the recombinant plasmid DNA was transformed into yeast AH109(α type).The transformed yeast AH109 was mated with yeast Y187(α type)containing liver cDNA library plasmid in 2×YPDA medium.Diploid yeast was plated on synthetic dropout nutrient medium(SD/-Trp-Leu-His-Ade)containing X-α-gal for selection and screening.After extracting and sequencing of plasmids from positive(blue)colonies,we made a sequence analysis by bioinformatics.Results We screened twenty-five proteins binding to NS5ABP37,including Homo sapiens cyclin I(CCNI)gene,Homo sapiens matrix metallopeptidase 25(MMP25)and Homo sapiens talin 1.Conclusion The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of HCV.And the biological function of NS5ABP37 may be associated with glycometabolism,lipid metabolism and apoptosis.

DECOUPLING CONTROL OF TWO MOTORS SYSTEM BASED ON NEURAL NETWORK INVERSE SYSTEM

In accordance with the characteristics of two motors system, the unitedmathematic model of two-motors inverter system with v/f variable frequency speed-regulating isgiven. Two-motor inverter system can be decoupled by the neural network invert system, and changedinto a sub-system of speed and a sub-system of tension. Multiple controllers are designed, and goodresults are obtained. Tie system has good static and dynamic performances and high anti-disturbanceof load.

Bursting oscillations in a hydro-turbine governing system with two time scales

A fast-slow coupled model of the hydro-turbine governing system （HTGS） is established by introducing frequency disturbance in this paper. Based on the proposed model, the performances of two time scales for bursting oscillations in the HTGS are investigated and the effect of periodic excitation of frequency disturbance is analyzed by using the bifurcation diagrams, time waveforms and phase portraits. We find that stability and operational characteristics of the HTGS change with the value of system parameter kd. Furthermore, the comparative analyses for the effect of the bursting oscillations on the system with different amplitudes of the periodic excitation a are carried out. Meanwhile, we obtain that the relative deviation of the mechanical torque mt rises with the increase of a. These methods and results of the study, combined with the performance of two time scales and the fast-slow coupled engineering model, provide some theoretical bases for investigating interesting physical phenomena of the engineering system.

Fault Diagnosis in a Hydraulic Position Servo System Using RBF Neural Network

Considering the nonlinea r, time-varying and ripple coupling properties in the hydraulic servo system, a two-stage Radial Basis Function （RBF） neural network model is proposed to realize the failure detection and fault localization. The first-stage RBF neural network is adopted as a failure observer to realize the failure detection. The trained RBF observer, working concurrently with the actual system, accepts the input voltage signal to the servo valve and the measurements of the ram displacements, rebuilds the system states, and estimates accurately the output of the system. By comparing the estimated outputs with the actual measurements, the residual signal is generated and then analyzed to report the occurrence of faults. The second-stage RBF neural network can locate the fault occurring through the residual and net parameters of the first-stage RBF observer. Considering the slow convergence speed of the K-means clustering algorithm, an improved K-means clustering algorithm and a self-adaptive adjustment algorithm of learning rate arc presented, which obtain the optimum learning rate by adjusting self-adaptive factor to guarantee the stability of the process and to quicken the convergence. The experimental results demonstrate that the two-stage RBF neural network model is effective in detecting and localizing the failure of the hydraulic position servo system.

Oscillatory and Asymptotic Behaviour of Solutions of Two Nonlinear Dimensional Difference Systems

This paper deals with the some oscillation criteria for the two dimensional difference system of the form: . Examples illustrating the results are inserted.

Singularity analysis of Duffing-van der Pol system with two bifurcation parameters under multi-frequency excitations

Bifurcation properties of a Duffing-van der Pol system with two parameters under multi-frequency excitations are studied. Three cases are discussed： （1） λ 1 is considered as bifurcation parameter, （2） λ 2 is considered as bifurcation parameter, and （3） λ 1 and λ 2 are both considered as bifurcation parameters. According to the definition of transition sets, the whole parametric space is divided into several different persistent regions by the transition sets for different cases. The bifurcation diagrams in different persistent regions are obtained, which provides a theoretical basis for optimal design of the system.

On the Gravitational Two-Body System and an Infinite Set of Laplace-Runge-Lenz Vectors

The current approach of a system of two bodies that interact through a gravitational force goes beyond the familiar expositions [1-3] and derives some interesting features and laws that are overlooked. A new expression for the angular momentum of a system in terms of the angular momenta of its parts is deduced. It is shown that the characteristics of the relative motion depend on the system’s total mass, whereas the characteristics of the individual motions depend on the masses of the two bodies. The reduced energy and angular momentum densities are constants of motion that do not depend on the distribution of the total mass between the two bodies;whereas the energy may vary in absolute value from an infinitesimal to a maximum value which occurs when the two bodies are of equal masses. In correspondence with infinite possible ways to describe the absolute rotational positioning of a two body system, an infinite set of Laplace-Runge-Lenz vectors (LRL) are constructed, all fixing a unique orientation of the orbit relative to the fixed stars. The common expression of LRV vector is an approximation of the actual one. The conditions for nested and intersecting individual orbits of the two bodies are specified. As far as we know, and apart from the law of periods, the laws of equivalent orbits concerning their associated periods, areal velocities, angular velocities, velocities, energies, as well as, the law of total angular momentum, were never considered before.