Dry Breeding and Dry Planting Techniques for Indica Hybrid Rice in Karst Mountain Areas of Gejiu City

Based on the arable land situation in Gejiu City,upland dry planting of indica hybrid rice is being expanded in Karst mountain areas with a rainfall of over 1400 mm and an altitude of 1100-1600 m to develop grain production.This paper gives a specific description of hybrid rice upland dry seedling technology,upland transplanting technology,fertilization technology,field management,weed prevention and control technology,and disease and pest control.

Solutions to Insecurity Problems in Seed Production of Two-line Hybrid Rice

The insecure problems of seed production have seriously hampered the healthy and sustainable development of two-line hybrid rice.The safety issues on seed production of two-line hybrid rice and current research situation were pointed out in this paper.The three main reasons for unsafety in seed production of twoline hybrid rice were unsuitable site selection,high critical sterility-inducing temperature and the drift of critical temperature.In this paper,strategies and measures were put forward based on many years of practice.It could minimize the risk in seed production of two-line hybrid rice by selecting dual-purpose genic male sterile line with lower critical sterility-inducing temperature and long lower temperature resistant time.Based on the climate data and climatic demands of the ＂three safe-periods＂ in seed production,a new idea for determining appropriate bases and periods for seed production was proposed by using computer technology,which solved the aimless selection of sites and periods for the seed production of two-line hybrid rice.Besides,we established a system of single plant selection and identification method and original seed propagation with cyclic cold water,which could reduce the generation number of original seed propagation in seed production and avoid the drift of critical sterility-inducing temperature.This paper improved the seed production safety in the three aspects of seed nature,seed source and seed production site.

Effects of Nitrogen Quantity on Grain-filling Characters of Two-line Hybrid Rice with Large Ear

[Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made use of to study on effects of N fertilizer in different quantities （LN： 90 kg/hm2;MN： 180 kg/hm2;HN： 270 kg/hm2） on plumpness and grain-filling characters. [Result] When N fertilizers were excessive, for inferior grains, grain-filling rate decreased and grain-filling time extended, resulting in plumpness decline after degradation of leaves＇ function. When N fertilizers were inadequate, maximal and average grain-filling rates decreased and the differences between superior and inferior grains in grain-filling rate increased, leading to decline of grain＇s weight and plumpness degree. On the other hand, quantity of N fertilizers had little effect on superior grains in plumpness. [Conclusion] The research provided references for reasonable use of N fertilizer and improvement of rice yield and N use.

Effects of SPAD Decline Value of Mid-season Hybrid Rice Leaves after Full-heading Stage on Productivity of Rice Fertilized with Nitrogen

[Objective] The aim was to research relationship between SPAD decline index after full-heading stage （SDIFHS） and productivity of rice. fertilized with nitro- gen in order to provide theoretical and practical references for selection and breed- ing of rice varieties. [Method] From 2008 to 2010, 18 mid-season hybrid rice vari- eties were researched every year to explore relationship＇ between SDIFHS and pro- ductivity of rice fertilized with nitrogen. [Result] The productivity of rice fertilized with nitrogen was of extremely significant positive corretation with SDIFHS, because the higher SPAD decline index is, the higher LAI decline index and the transformation ratio of dry matter to spikes in overground plant would be. [Conclusion] The re- search established a new method to predict productivity of rice fertilized with nitro- gen based on SPAD decline index.

Study on Agronomic Characters and Storage Durability of a New Line of Endurable Storage Hybrid Rice Chucangyou No.1

[Objective] The aim of this study was to investigate the storage durability of a new line of endurable storage hybrid rice Chucangyou No.1 selected by endurable storage genes of Yunhui 290.[Method] Agronomic characters of the new line and the control II you 838 and Shanyou 63 were comparatively studied,and storage durability of the new line and indica,japonica or hybrid rice were also comparatively analyzed by the accelerated ageing test.[Result] Agronomic characters of the new line were excellent,and germination rate in the artificial accelerated ageing test was significantly higher than those in other materials.[Conclusion] Chucangyou No.1 has the better storage durability,which can be used in large scale of extention.

Analysis of Heterosis, Combining Ability and Heritability of Cadmium Content in Brown Rice of Three-line Indica Hybrid Rice

Five cytoplasmic male sterile （CMS） lines were used as parents in an incomplete diallet cross and six restorer lines of rice design （Nc II design）. Thirty hybrid combinations with the same growth period were selected as experimental ma- terials to investigate the heterosis, combined ability and heredity of Cd content in brown rice of indica hybrid rice. According to the results, Cd content in brown rice showed a significantly negative heterosis; the general combining ability and specific combination ability of Cd content in CMS and restorer lines both reached extremely significant level （P〈0.01）, indicating that both genetic improvement of parents and e- valuation of combinations are important to the breeding of hybrid combinations with low accumulation of Cd; the broad-sense heritabitity and narrow-sense heritability of Cd content were both relatively high with slight differences, which respectively reached 97.73% and 80.10%, indicating that Cd content in brown rice mainly de- pends on the additive action of genes; in addition, parent improvement showed bet- ter effect on the selection of early generation.

Combining Ability Analysis on Yield Traits of Recombinant Inbred Lines in Hybrid Rice

A population of 140 recombinant inbred lines at F8 generation were obtained after seven successive generations of self-pollination using single seed descent（SSD） method from the F2 hybrids of three-line restorers Luhui 8258 with high combining ability and Yanghui 34. Then, the 140 inbred lines obtained above and their parents Luhui 8258 and Yanghui 34 were crossed with three different types of cyto-plasmic male sterile（CMS） lines（Gang 46 A, Ⅱ-32 A and Lu 98A） according to NCⅡ design. The resulting 426 combinations were planted at Deyang and Suining bases to test the combining ability and inheritance of five yield traits： yield per plant, panicle number per plant, filled grain number per panicle, seed setting rate and 1 000-grain weight. The results showed that the variances of both general and specific combining abilities of the five traits all reached a significant or extremely significant level at the two sites. The broad and narrow heritability of the yield traits（except 1 000-grain weight whose broad and narrow heritability were both over70%） were all below 50% at the two experimental bases, suggesting that the four traits were easily subjected to environment influence. Very significant positive correlation of general combining ability was found between yield per plant and other traits except 1 000-grain weight. The general combining ability variance showed a normal distribution among the recombinant inbred lines at two sites, right in line with inheritance of quantitative traits. So, the genes controlling rice general combining ability can be targeted by QTL mapping.

High-yielding Seed Production Techniques of Disease-resistant and High-quality Hybrid Rice Sterile Line Rong 18A

Rong 18A, bred by Crop Research Institute, Chengdu Academy of Agri- culture and Forestry Sciences is a new blast-highly resistant and high-combining ability hybrid rice sterile line. It is currently one of the male sterile lines in Sichuan Province which combine well high grain quality and rice blast resistance. More than 20 combinations of Rong 18A have passed the national and provincial variety iden- tification. Combined with the climate conditions in Chengdu, the key points of high- yielding seed production techniques for Rong18A are analyzed and summarized in this paper.

Effect of Enhanced UV-B Radiation on Seed Setting Rate and 1 000-grain Weight of Indica Hybrid Rice Restorer Lines

[Objective] This study was to investigate the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight of hybrid rice combinations. [Method] The seed setting rate and 1 000-grain weight of 10 new sterile indica restorer lines planted in pots under enhanced UV-B radiation and fluorescent lamps （control） were respectively measured, and the differences were compared. [Result] The enhanced UV-B radiation significantly reduced the seed setting rate of indica restorer lines, and the differences between that UV-B radiation treatment and control all reached extremely significant level. In addition, the enhanced UV-B radiation reduced the 1 000-grain weight of most indica restorer lines, and compared with that of control the difference achieved significant or very significant level. However, the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight differed to different indica restorer lines, and the differences among restorer lines tested were significant or very significant, which indicated the possibility to screen antiUV-B radiation rice materials and combinations. Finally, the indica restorer lines 09R-14, Luhui 37 and 10R-7703 which were strongly resistant to UV-B radiation were screened out. [Conclusion] This study laid foundation for breeding hybrid rice varieties resistance to UV-B radiation.

Breeding and Application of Indica PTGMS Line Yan 161S and Its Hybrid Yanliangyou 1618 in Rice

In 2006, the indica photoperiod- and thermo-sensitive genic male sterile （PTGMS） line Yan 161S was successful y bred by crossing the Guangzhan 63S （♀） and the medium indica breeding material Yan 229 （♂）, which was bred by the Institute of Agricultural Sciences in Coastal Region of Jiangsu Province, after years＇ and generations’ selection in different areas. It passed the identification by Jiangsu Provincial Crop Variety Appraisal Committee in 2013. Crossing the Yan 161S with Yanhui 888, a new high-yielding and high-quality two-line hybrid rice combination Yanliangyou 1618 was developed, and it passed the variety identification in 2014. This paper introduced the breeding processes and characteristics of Yan 161S and Yanliangyou 1618 and the cultivation and seed production techniques of Yanliangyou 1618.

Breeding of National Authorized Two-line Hybrid Rice Yanliangyou 2208

The breeding processes, characteristics and comprehensive characters of two-line hybrid rice Yanliangyou 2208 and its parents （Yan 220S and Yanhui 888） taking part in the national rice regional test of southern rice regions were introduced. Yanliangyou 2208 was approved by the National Crop Variety Appraisal Committee in 2013. The achievement has been transformed successfully and has been applied in production. It has good development and promotion prospects.

A Preliminary Study on Nitrogen Use Efficiency of Hybrid Rice Combinations Derived from CMS Line Rong 18A

In order to investigate characteristics of nitrogen use efficiency of CMS line Rong 18A, four hybrid rice combinations （Rong18 You188, Rong18 You198, Rong18 You662, Rong18 You908） derived from CMS line Rong 18A were used as experimental materials with Ⅱ You838 and Gangyou725 as controls. A split-plot experimental design was performed with four nitrogen fertilizer application rates to analyze the effects of different nitrogen fertilizer application rates on the yield and yield component factors of rice. The results showed that nitrogen fertilizer could significantly increase effective panicle number to improve rice yield. The increment in rice yield per unit nitrogen fertilizer application rate demonstrated a descending trend with the increase of nitrogen fertilizer application rate. Under low nitrogen levels, hybrid rice combinations derived from Rong 18A exhibited relatively high nitrogen use efficiency and showed remarkably high nitrogen use efficiency under low nitrogen levels.

Seed Production Techniques of Two-line Indica Hybrid Rice Yanliangyou 2208

Yanliangyou 2208, bred by the Jiangsu Institute of Agricultural Sciences in Coastal Areas, is a two-line indica hybrid rice combination characterized with strong heterosis, moderate maturity, wide adaptability, good stability and disease resistance. It was identified as a new variety by the National Crop Variety Approval Committee in 2013. According to the characteristics of parents of Yanliangyou 2208 and com- bined with many years of seed production experience, high-yielding seed production techniques of Yanliangyou 2208 are summed up in this paper.

Effect of Different Fertilization Methods on Grain Yield and Nitrogen Agronomy Efficiency of TwoLine Super Hybrid Rice

ln order to explore the effect of broadcast application of urea （BR-U） and surface concentrated-fertilization on grain yield and nitrogen agronomy efficiency of two-line super hybrid rice, this experiment was conducted with super hybrid rice Y-Liangyou 1 and Liangyou 0293 to determine til ering dynamics, SPAD, LAl and dry matter accumulation. lts total N application was 180 kg/hm2 and different application rate and topdressing methods were set respectively. The results showed that grain yield and nitrogen agronomy efficiency were significantly improved with the T3 and T4 nitrogenous fertilizer application model. The improving of grain yield and nitrogen agronomy efficiency was benefited from rational postponing N application. However, neither single application of base fertilizer （T2） nor single application of topdressing fertilizer （T5 and T6） can coordinate the relationship between source and sink. Both grain yield and nitrogen agronomy efficiency were insignificantly under the broadcast application of urea and surface concentrated fertilization.

Grain yield,nitrogen use efficiency and physiological performance of indica/japonica hybrid rice in response to various nitrogen rates

Utilizing the heterosis of indica/japonica hybrid rice(IJHR)is an effective way to further increase rice grain yield.Rational application of nitrogen(N)fertilizer plays a very important role in using the heterosis of IJHR to achieve its great yield potential.However,the responses of the grain yield and N utilization of IJHR to N application rates and the underlying physiological mechanism remain elusive.The purpose of this study was to clarify these issues.Three rice cultivars currently used in rice production,an IJHR cultivar Yongyou 2640(YY2640),a japonica cultivar Lianjing 7(LJ-7)and an indica cultivar Yangdao 6(YD-6),were grown in the field with six N rates(0,100,200,300,400,and 500 kg ha^(-1))in 2018 and 2019.The results showed that with the increase in N application rates,the grain yield of each test cultivar increased at first and then decreased,and the highest grain yield was at the N rate of 400 kg ha^(-1)for YY2640,with a grain yield of 13.4 t ha^(-1),and at 300 kg ha^(-1)for LJ-7 and YD-6,with grain yields of 9.4–10.6 t ha^(-1).The grain yield and N use efficiency(NUE)of YY2640 were higher than those of LJ-7 or YD-6 at the same N rate,especially at the higher N rates.When compared with LJ-7 or YD-6,YY2640 exhibited better physiological traits,including greater root oxidation activity and leaf photosynthetic rate,higher cytokinin content in the roots and leaves,and more remobilization of assimilates from the stem to the grain during grain filling.The results suggest that IJHR could attain both higher grain yield and higher NUE than inbred rice at either low or high N application rates.Improved shoot and root traits of the IJHR contribute to its higher grain yield and NUE,and a higher content of cytokinins in the IJHR plants plays a vital role in their responses to N application rates and also benefits other physiological processes.

Characterization and evaluation of rice blast resistance of Chinese indica hybrid rice parental lines

The development of resistant varieties and hybrid combinations has been the most effective and economical strategy to control blast disease caused by Magnaporthe oryzae.However, the distribution of major R genes and blast resistance characterization in hybrid rice parents has not been well investigated, resulting in their limited use in hybrid rice blast-resistance breeding. In the present study, 88 elite indica hybrid rice parental lines were evaluated with 30 isolates of M. oryzae collected from the main planting area of indica hybrid rice in China and were characterized for the presence of 11 major resistance genes using molecular markers. The pathogenicity assays showed that four types of hybrid rice parent line showed some resistance to M. oryzae. However, the proportions of highly resistant lines and the mean resistance frequency(RF) varied among the four types, with resistance in decreasing order shown by three-line restorer lines, three-line maintainer lines, two-line sterile lines, and two-line restorer lines. All 88 hybrid rice parental lines carried more than one R gene, but none carried the R genes Pi1 and Pi2. Although Pid3 and Pi9 were present only in three-line restorer lines and Pigm only in three-line maintainer lines, the remaining six R genes(Pib, Pid2, Pi5, Pia, Pi54, and Pita) were present in the four types of hybrid rice parent with significantly different distribution frequencies. The correlation between R genes and resistance reactions was investigated. The results are expected to provide useful information for rational utilization of major R genes in hybrid rice breeding programs.

Thoughts and Practice on Some Problems about Research and Application of Two-Line Hybrid Rice

The main problems about research and application of two-line hybrid rice were reviewed, including the confusing nomenclature and male sterile lines classification, the unclear characteristics of photoperiod and temperature responses and the unsuitable site selection for male sterile line and hybrid dce seed production. In order to efficiently and accurately use dual-purpose genic male sterile lines, four types, including PTGMS （photo-thermo-sensitive genic male sterile rice）, TGMS （thermo-sensitive genic male sterile rice）, reverse PTGMS and reverse TGMS, were proposed. A new idea for explaining the mechanism of sterility in dual-purpose hybrid rice was proposed. The transition from sterile to fertile was involved in the cooperative regulation of major-effect sterile genes and photoperiod and/or temperature sensitive ones. The minor-effect genes with accumulative effect on sterility were important factors that affected the critical temperature of sterility transfer. In order to make better use of dual-purpose lines, the characterization of responses to photoperiod and temperature of PTGMS should be made and the identification method for the characterization of photoperiod and temperature responses of PTGMS should also be put forward. The optimal ecological site for seed production could be determined according to the historical climate data and the requirements for the meteorological conditions during the different periods of seed production.

Studies on Seed Setting Stability in Associated with Temperature for a Two-Line Hybrid Rice,Liangyoupeijiu

When being planted in wide areas in southern China (23°23' - 33°23' N, 98°35' - 120°19' E, sea level above 2.7 - 1285 m), Liangyoupeijiu (LYPJ), a two-line hybrid rice combination, showed a seed setting rate of 75.2 - 77.2%, which was lower by 4.3 - 7.5 percent point than that of an indica hybrid rice Shanyou 63 (CK), with similar values of grain yield and coefficients of variation to CK. Sowing during 5-25th of May in Nanjing (32°3' N, 118°48' E), China, LYPJ headed before 4th September, and gave a seed setting rate of 75 - 90%, and grain yield over 1 kg/m2. If the sowing date was delayed to 14 - 15th, June, its heading date would be as late as 17th - 21st September, and seed setting rate would be declined by 10 - 15% in comparison with that on a suitable sowing date. When flowering took place at an average daily temperature range of 13.7 - 28.5℃ for five days, the spikelet fertility (SF) would be increased by 1.9- 10.7%, for each increment of 1℃. The suitable (SF≥90%) and safe (SF≥75%) temperatures for flowering stage were indicated to be 26.5℃ and 22.9℃, respectively. To get a high and stable seed set in cultivating LYPJ, it was recommended that LYPJ would be planted in the areas south of 34癗, and the favorable average daily temperature during flowering stage should be at 26 - 28℃.

Source-Sink and Grain-Filling Characteristics of Two-Line Hybrid Rice Yangliangyou 6

With two-line hybrid rice Yangliangyou 6 （YLY6） and Liangyoupeijiu （LYPJ） and three-line hybrid rice Shanyou 63 （SY63） as materials, the source, sink and flow characteristics in association with grain filling were investigated. The seed-setting rate, grain filling degree and grain yield of YLY6 and SY63 were significantly higher than those of LYPJ. The export and transformation percentages of the matter in culms and sheaths of YLY6 and SY63 were significantly higher than those of LYPJ. Activities of sucrose synthase, adenosine diphosphoglucose pyrophosphorylase, starch synthase and starch branching enzyme in grains were higher for YLY6 and SY63 than for LYPJ, and were very significantly correlated with maximum grain filling rate, mean grain filling rate, grain filling degree and grain weight. The spikelet number, grain yield and total sink load per area of vascular bundle and phloem of YLY6 and SY63 were significantly smaller than those of LYPJ, and the greater the load, the lower seed-setting rate and the poorer grain filling. The transportation rate per area phloem of YLY6 was greater than that of LYPJ or SY63. The results suggest that YLY6 possesses strong source, great sink activity and efficient flow, which lay a physiological base for its high seed-setting rate and good grain filling.

Resistance Evaluation of Some Chinese Leading Rice Maintainer, Restorer lines and Their Hybrids to Magnaporthe grisea

Six isolates of Magnaporthe grisea were selected to inoculate on 10 Chinese leading maintainer lines (B-lines), 14 restorer lines (R-lines) and their F1 hybrid plants. In the tested rice materials, R-lines were proved to be more resistant to blast than B-lines. The resistance frequency of about 25% F1 hybrid plants was less than their parents. In addition, 26 isolates of M. grisea collected from different rice growing areas of China were inoculated on 13 new improved hybrid rice combinations. The resistance frequencies of 5 improved hybrids were better than those of the controls and leading varieties in rice production of China.