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TURBULENT SEPARATED REATTACHED FLOW IN A TWO-DIMENSIONAL CURVED-WALL DIFFUSER
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作者 尹军飞 余少志 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 1993年第2期117-123,共7页
A turbulent separation-rcattachment flow in a two-dimensional asymmetrical curved-wall diffuser is studied by a two-dimensional laser doppler velocimeter.The turbulent boundary layer separates on the lower curved wall... A turbulent separation-rcattachment flow in a two-dimensional asymmetrical curved-wall diffuser is studied by a two-dimensional laser doppler velocimeter.The turbulent boundary layer separates on the lower curved wall under strong pressure gradient and then reattaches on a parallel channel.At the inlet of the diffuser,Reynolds number based on the diffuser height is 1.2×10~5 and the velocity is 25.2m/s.The re- sults of experiments are presented and analyzed in new defined streamline-aligned coordinates.The experiment shows that after Transitory Detachment Reynolds shear stress is negative in the near-wall backflow region. Their characteristics are approximately the same as in simple turbulent shear layers near the maximum Reynolds shear stress.A scale is formed using the maximum Reynolds shear stresses.It is found that a Reynolds shear stress similarity exists from separation to reattachment and the Schofield-Perry velocity law ex- ists in the forward shear flow.Both profiles are used in the experimental work that leads to the design of a new eddy-viscosity model.The length scale is taken from that developed by Schofield and Perry.The composite velocity scale is formed by the maximum Reynolds shear stress and the Schofield Perry velocity scale as well as the edge velocity of the boundary layer.The results of these experiments are presented in this paper 展开更多
关键词 separating flow boundary layer turbulent flow turbulence model Laser Doppler Velocimeter two- dimensional diffuser
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胎鼠肌组织蛋白质组的双向电泳分析 被引量:1
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作者 周海涛 富伟能 +1 位作者 金春莲 孙开来 《中国公共卫生》 CAS CSCD 北大核心 2006年第9期1087-1088,共2页
目的优化胚胎鼠肌组织蛋白质组双向电泳分析方法。方法以固相pH梯度胶条等电聚焦为第1向,以十二烷基磺酸钠(SDS)聚丙烯酰胺凝胶电泳为第2向,对孕21 d大鼠胚胎胫骨后肌总蛋白进行双向电泳,经银染或考马斯亮蓝染色,并采用ImageMaster 5.0... 目的优化胚胎鼠肌组织蛋白质组双向电泳分析方法。方法以固相pH梯度胶条等电聚焦为第1向,以十二烷基磺酸钠(SDS)聚丙烯酰胺凝胶电泳为第2向,对孕21 d大鼠胚胎胫骨后肌总蛋白进行双向电泳,经银染或考马斯亮蓝染色,并采用ImageMaster 5.0软件分析处理。在不同的3张胶上选一对应点进行质谱分析和网络数据检索鉴定。结果获得了分辨率及重复性均较好的二维图谱。对应点质谱数一致。结论通过条件优化双向电泳结果能满足胎鼠肌组织蛋白质点分析鉴定需要。 展开更多
关键词 双向电泳 蛋白质组 大鼠胎胚
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Separation and Identification of Differentially Expressed Proteins in Pistillate Flowers between Different Mulberry Cultivars
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作者 牛瑞鹤 陈媛媛 +3 位作者 张萍萍 祁伟 郑必平 谈建中 《Agricultural Science & Technology》 CAS 2013年第10期1383-1385,1402,共4页
[Objective] This study aimed to explore the proteins related to pistillate flower development in different mulberry cultivars. [Method] The total proteins of the pistillate flowers of two mulberry cultivars Dal0 (Mor... [Objective] This study aimed to explore the proteins related to pistillate flower development in different mulberry cultivars. [Method] The total proteins of the pistillate flowers of two mulberry cultivars Dal0 (Morus atropurpurea Roxb.) and SG01 (Morus muIticaulis Perr.) were extracted, separated and detected through two- dimensional electrophoresis (2-DE) and mass spectrometry. [Result] There was sig- nificant difference in the expression of proteins from the pistillate flowers of different mulberry cultivars. From the 2-DE images of Dal0 and SG01, 445_+17 and 425_+12 protein spots were respectively detected. The expression levels of 75 protein spots differed significantly. Thirteen spots those were expressed at high levels and well separated were analyzed by mass spectrometry, and nine of them were identified successfully. The nine proteins are involved in the glycometabolism, protein and amino acid metabolism and defense responses during the development of mulberry pistillate flower after they were pollinated. [Conclusion] The findings will provide reference for further study on the molecular mechanism of mulberry pistillate flower de- velopment. 展开更多
关键词 Mulberry cultivars Pistillate flower Differentially expressed proteins two- dimensional electrophoresis Mass spectrometry
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蛋白质组学在发育生物学研究中的应用 被引量:1
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作者 喻小燕 曹晶 谢锦云 《金陵科技学院学报》 2006年第4期80-86,90,共8页
发育生物学主要研究生物体从精子和卵子的发生、受精、胚胎发育、生长到衰老、死亡的规律。一个生物体的蛋白质随发育阶段、特定组织等变迁而不断变化,蕴藏着巨大的动态的生命活动的信息量,利用蛋白质组学技术可从分子水平了解生物体的... 发育生物学主要研究生物体从精子和卵子的发生、受精、胚胎发育、生长到衰老、死亡的规律。一个生物体的蛋白质随发育阶段、特定组织等变迁而不断变化,蕴藏着巨大的动态的生命活动的信息量,利用蛋白质组学技术可从分子水平了解生物体的发育调控、发育异常等的机制,具有重要的意义和广泛的应用前景。综述了蛋白质组学主要研究方法及其在动物和植物发育生物学中的应用。 展开更多
关键词 蛋白质组学 发育 双向凝胶电泳
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蛋白质组学在糖尿病研究中的应用
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作者 杨丕坚 夏宁 梁瑜祯 《医学综述》 2008年第5期736-738,共3页
当今分子生物学领域内,蛋白质组已成为研究的热点。基因组相对较稳定,而且各种细胞或生物体的基因组结构有许多基本相似的特征;蛋白质组是动态的,随内外界刺激而变化。对蛋白质组的研究可以使人类更容易接近对生命过程的认识。简要介绍... 当今分子生物学领域内,蛋白质组已成为研究的热点。基因组相对较稳定,而且各种细胞或生物体的基因组结构有许多基本相似的特征;蛋白质组是动态的,随内外界刺激而变化。对蛋白质组的研究可以使人类更容易接近对生命过程的认识。简要介绍蛋白质组学这一新兴学科的基本概念和技术流程,着重阐述其在糖尿病的研究进展。 展开更多
关键词 蛋白质组学 糖尿病 双向电泳
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A coincidence experimental setup for investigating multiple electron processes 被引量:1
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作者 马新文 刘惠萍 +7 位作者 王友德 杨治虎 王树金 陈熙萌 申自勇 吕魁 蔡晓红 刘兆远 《Science China Mathematics》 SCIE 1998年第3期296-300,共5页
The coincidence experimental setup for studying multiple electron processes at low energies established at the Institute of Modern Physics was presented. The setup includes time-of-flight spectrometer, position sensit... The coincidence experimental setup for studying multiple electron processes at low energies established at the Institute of Modern Physics was presented. The setup includes time-of-flight spectrometer, position sensitive detector, and multi-parameter data acquisition system. Presented were a TOF spectrum for Ar 12+ on Ar collisions, a position spectrum obtained in O 2++He collisions, and a two-dimension spectrum for Ar 8++Ar collisions at 104 keV. The experimental results were analyzed in detail and 12 subprocesses were identified from the two-dimension spectrum. 展开更多
关键词 MULTIPLE ELECTRON transfer COINCIDENCE technique position sensitive detector TIME-OF-FLIGHT spectrome- TER two- dimension spectrum.
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