INVARIANT SUBSPACES AND GENERALIZED FUNCTIONAL SEPARABLE SOLUTIONS TO THE TWO-COMPONENT b-FAMILY SYSTEM

Invariant subspace method is exploited to obtain exact solutions of the two- component b-family system. It is shown that the two-component b-family system admits the generalized functional separable solutions. Furthermore, blow up and behavior of those exact solutions are also investigated.

Notes on well-posedness for the b-family equation

The local well-posedness for the b-family equation in the critical space is studied.Applying the Littlewood-Paley decomposition method in the critical Besov spaces Bs2r with the index s=3/2 which is the generalization space of the Sobolev spaces Hs it is established that there is a maximal time T=Tu0＆gt;0 such that for the b-family equation there exists a unique solution utx∈C0TB3/221∩C10TB121 when the initial variable u0x ∈B3/221 is the critical regularity. Moreover the solution uxt depends continuously on the initial data u0x .Furthermore using the abstract Cauchy-Kowalevski theorem to prove the analytic regularity of the solutions for the b-family equation in a suitable scale of Banach spaces E it is shown that the solutions of the b-family are analytic in both variables globally in space and locally in time when the initial data is analytic.

柴胡疏肝汤对癫痫大鼠海马组织中Bcl-2、NLRP1、Caspase-3、Caspase-1表达的影响

目的探讨柴胡疏肝汤对癫痫大鼠海马组织中B细胞淋巴瘤2(Bcl-2)、含NLR家族pyrin结构域1因子(NLRP1)、Caspase-3、Caspase-1表达的影响。方法基于生物信息学分析筛选柴胡疏肝汤治疗癫痫的核心活性成分及核心靶点。将100只SD大鼠随机分为癫痫组(n=80)与空白组(n=20)。对空白组大鼠不作任何处理,对癫痫组大鼠建立癫痫模型,成功建模后将其随机分为模型组、柴胡疏肝汤低剂量组、柴胡疏肝汤中剂量组、柴胡疏肝汤高剂量组,每组20只。给予柴胡疏肝汤低、中、高剂量组大鼠柴胡疏肝汤(2.5 mg/100 g、5 mg/100 g、10 mg/100 g)灌胃,给予模型组及空白组大鼠生理盐水灌胃。给药4周后,比较模型组与柴胡疏肝汤低、中、高剂量组的大鼠癫痫发作等级,然后处死5组大鼠并获取其海马组织,分别采用实时荧光定量PCR和Western blot检测Bcl-2、NLRP1、Caspase-3、Caspase-1的mRNA及蛋白表达水平。结果(1)柴胡疏肝汤治疗癫痫的核心活性成分包括槲皮素、山柰酚、柚皮素、β-谷甾醇、川陈皮素、异鼠李素、木犀草素、豆甾醇、7-甲氧基-2-甲基异黄酮、罗通定,核心靶点包括Bcl-2、NLRP1、Caspase-3、Caspase-1。槲皮素、山柰酚、柚皮素、β-谷甾醇、川陈皮素和木犀草素与核心靶点表现出较好的结合活性。(2)干预后,与模型组相比,柴胡疏肝汤中、高剂量组的癫痫发作等级降低(P<0.05);与柴胡疏肝汤低剂量组相比,柴胡疏肝汤中、高剂量组的癫痫发作等级降低(P<0.05)。与空白组比较,模型组Bcl-2的mRNA和蛋白表达水平降低,NLRP1、Caspase-3、Caspase-1的mRNA和蛋白表达水平升高(P<0.05);与模型组比较,柴胡疏肝汤各剂量组Bcl-2的m RNA和蛋白表达水平升高,NLRP1、Caspase-3、Caspase-1的mRNA和蛋白表达水平降低(P<0.05);柴胡疏肝汤低剂量组、柴胡疏肝汤高剂量组、柴胡疏肝汤中剂量组Bcl-2的mRNA和蛋白表达水平依次升高,柴胡疏肝汤中剂量组、柴胡疏肝汤低剂量组、柴胡疏肝汤高剂量组NLRP1的m RNA和蛋白表达水平依次升高,柴胡疏肝汤高剂量组、柴胡疏肝汤低剂量组、柴胡疏肝汤中剂量组Caspase-3、Caspase-1的mRNA和蛋白表达水平依次升高(P<0.05)。结论柴胡疏肝汤可能通过上调大鼠海马组织中Bcl-2的表达,抑制NLRP1、Caspase-3、Caspase-1的表达,发挥抗癫痫作用。

Effects of sex and generation on hepatitis B viral load in families with hepatocellular carcinoma

AIM To explore factors associated with persistent hepatitis B virus (HBV) infection in a cohort of hepatocellular carcinoma (HCC)-affected families and then investigate factors that correlate with individual viral load among hepatitis B surface antigen (HBsAg)-positive relatives. METHODS questionnaire. Demographics, relationship to index case, HBsAg status of mothers and index cases were evaluated for association with the HBV persistent infection or viral load by generalized estimating equation analysis. RESULTS Among 729 relatives enrolled, parent generation (P = 0.0076), index generation (P = 0.0044), mothers positive for HBsAg (P = 0.0007), and HBsAg-positive index cases (P = 5.98 x 10(-8)) were associated with persistent HBV infection. Factors associated with HBV viral load were evaluated among 303 HBsAg-positive relatives. Parent generation (P = 0.0359) and sex (P = 0.0007) were independent factors associated with HBV viral load. The intra-family HBV viral load was evaluated in families clustered with HBsAg-positive siblings. An intra-family trend of similar HBV viral load was found for 27 of 46 (58.7%) families. Male offspring of HBsAg-positive mothers (P = 0.024) and older siblings were associated with high viral load. CONCLUSION Sex and generation play important roles on HBV viral load. Maternal birth age and nutritional changes could be the reasons of viral load difference between generations.

蓝莓B-box型锌指蛋白基因家族的鉴定及其在果实发育期的表达模式

B-box(BBX)基因家族成员在植物生长发育中起重要作用.为探究BBX在蓝莓果实成熟发育过程中的作用,通过生物信息学的方法鉴定BBX基因家族成员,并对其进行染色体定位、系统进化关系、基因结构、启动子顺式作用元件及表达模式分析.结果表明:在蓝莓基因组中鉴定出38个BBX基因成员,基因结构保守,外显子平均个数为3.13,这些基因可分成Ⅰ~Ⅲ3个亚族,不均匀分布于23条染色体;BBX基因的启动子序列中存在光响应、植物激素响应、胁迫响应和植物生长发育等4类顺式作用元件;VcBBX基因成员在‘Star’和‘O’Neal’蓝莓果实发育过程中存在显著性差异表达,VcBBX9,VcBBX16,VcBBX25和VcBBX20在S8表达量最高,VcBBX26在S6表达量最高,VcBBX30在S3表达量最高.研究结果将为进一步探究BBX型锌指蛋白在蓝莓果实发育过程中的功能奠定基础.

Asymptotically Optimal Empirical Bayes Estimation of Parameter for Scale-exponential Family under PA Samples

The Bayes estimator of the parameter is obtained for the scale exponential family in the case of identically distributed and positively associated（PA） samples under weighted square loss function.We construct the empirical Bayes（EB） estimator and prove it is asymptotic optimal.

Genome-wide identification and in silico gene expression analysis of the related to ABI3/VP1(RAV)transcription factor family in barley(Hordeum vulgare L.)

RAV(Related to ABI3/VP1)transcription factors are unique members of the AP2-ERF superfamily with AP2 and B3 domains and play important roles in the regulation of seed germination,plant growth,and stress response.In the study,7 RAV genes,named HvRAVs,were identified in barley based on the available genome sequences.While five of the seven HvRAVs were located on chromosome 3,HvRAV5 and HvRAV7 were located on chromosome 1 and 4,respectively.Six of the predicted HvRAVs were intron-less,except HvRAV2,which had one intron.HvRAV proteins have shown basic,instable,and hydrophilic properties.The AP2 domain specific RAYD and WLG motifs were detected in all HvRAV proteins.Besides,B3 repression domain,R/KLFGV,is also found in the C-terminal of HvRAVs.HvRAVs were found to have stress-related cis-acting elements,including MYB,MYC,and W-BOX.HvRAV2 was predicted to have no GARE motifs,TATCCCA or TAACAA(G/A),and LTREs.Under drought conditions,the expression level of HvRAVs did not significantly change in a drought-sensitive barley genotype,whereas HvRAV5 and HvRAV7 were dramatically down-regulated in a drought-tolerant genotype.Expression of HvRAV5 was also inhibited by salinity.HvRAV7 was strongly induced by plant pathogen attack.Only HvRAV6 was induced by exogenous gibberellin application and during the germination process.Interestingly,HvRAV6 transcript was detected higher than other HvRAVs in all stress and control conditions as well as during germination.In silico analyses have shown that HvRAVs play a role in response to different abiotic and biotic stresses as well as in plant development.However,the extensive biological roles of HvRAV genes in plant development and in response to abiotic and biotic stresses need further investigation.

Family with sequence similarity 134 member B-mediated reticulophagy ameliorates hepatocyte apoptosis induced by dithiothreitol

BACKGROUND Endoplasmic reticulum(ER)stress-related hepatocyte apoptosis is responsible for multiple hepatic diseases.Previous studies have revealed that endoplasmic reticulophagy(ER-phagy)promotes the selective clearance of damaged ER fragments during ER stress,playing a crucial role in maintaining ER homeostasis and inhibiting apoptosis.Family with sequence similarity 134 member B(FAM134B)is a receptor involved in ER-phagy that can form a complex with calnexin(CNX)and microtubule-associated protein 1 light chain 3(LC3).The complex can mediate the selective isolation of ER fragments to attenuate hepatocyte apoptosis.However,the precise regulatory mechanisms remain unclear.AIM To elucidate the effect of FAM134B-mediated ER-phagy on ER stress-induced apoptosis in buffalo rat liver 3A(BRL-3A)rat hepatocytes and the potential regulatory mechanisms.METHODS ER stress-related hepatocyte apoptosis was induced using dithiothreitol(DTT).Proteins related to ER stress and autophagy were measured with western blotting.Protein complex interactions with FAM134B were isolated by co-immunoprecipitation.ER-phagy was evaluated in immunofluorescence experiments.Cell cycle distribution and apoptosis were measured by flow cytometry.Mitochondrial Ca^(2+) levels were evaluated by the co-localization of intracellular Ca^(2+)-tracker and Mitotracker.The small interfering RNA against FAM134B was used to knockdown FAM134B in BRL-3A cells.RESULTS ER stress-related and autophagy-related proteins in BRL-3A cells were elevated by both short and long-term DTT treatment.Furthermore,co-immunoprecipitation confirmed an interaction between FAM134B,CNX,FAM134B,and LC3 in BRL-3A cells.Immunofluorescence assays revealed that autolysosomes significantly decreased following short-term DTT treatment,but increased after long-term treatment.Mitochondrial Ca2+levels and apoptotic rates were dramatically elevated,and more cells were arrested in the G1 stage after short-term DTT treatment;however,these decreased 48 h later.Moreover,FAM134B downregulation accelerated mitochondrial apoptotic pathway activation and aggravated hepatocyte apoptosis under ER stress.CONCLUSION FAM134B-mediated ER-phagy attenuates hepatocyte apoptosis by suppressing the mitochondrial apoptotic pathway.Our findings provide new evidence highlighting the importance of FAM134Bmediated ER-phagy in attenuating hepatocyte apoptosis.

Jianpi Gushen Huayu decoction ameliorated diabetic nephropathy through modulating metabolites in kidney,and inhibiting TLR4/NF-κB/NLRP3 and JNK/P38 pathways

BACKGROUND Jianpi Gushen Huayu Decoction(JPGS)has been used to clinically treat diabetic nephropathy(DN)for many years.However,the protective mechanism of JPGS in treating DN remains unclear.AIM To evaluate the therapeutic effects and the possible mechanism of JPGS on DN.METHODS We first evaluated the therapeutic potential of JPGS on a DN mouse model.We then investigated the effect of JPGS on the renal metabolite levels of DN mice using non-targeted metabolomics.Furthermore,we examined the effects of JPGS on c-Jun N-terminal kinase(JNK)/P38-mediated apoptosis and the inflammatory responses mediated by toll-like receptor 4(TLR4)/nuclear factor-kappa B(NF-κB)/NOD-like receptor family pyrin domain containing 3(NLRP3).RESULTS The ameliorative effects of JPGS on DN mice included the alleviation of renal injury and the control of inflammation and oxidative stress.Untargeted metabolomic analysis revealed that JPGS altered the metabolites of the kidneys in DN mice.A total of 51 differential metabolites were screened.Pathway analysis results indicated that nine pathways significantly changed between the control and model groups,while six pathways significantly altered between the model and JPGS groups.Pathways related to cysteine and methionine metabolism;alanine,tryptophan metabolism;aspartate and glutamate metabolism;and riboflavin metabolism were identified as the key pathways through which JPGS affects DN.Further experimental validation showed that JPGS treatment reduced the expression of TLR4/NF-κB/NLRP3 pathways and JNK/P38 pathway-mediated apoptosis related factors.CONCLUSION JPGS could markedly treat mice with streptozotocin(STZ)-induced DN,which is possibly related to the regulation of several metabolic pathways found in kidneys.Furthermore,JPGS could improve kidney inflammatory responses and ameliorate kidney injuries in DN mice via the TLR4/NF-κB/NLRP3 pathway and inhibit JNK/P38 pathwaymediated apoptosis in DN mice.

基于COM-B模型护理干预在脑卒中患者家庭支持/照护依赖方面的影响

目的:探讨基于COM-B模型的护理干预在中青年脑卒中患者家庭支持、照护依赖方面的影响。方法:研究纳入在医院治疗的80例中青年脑卒中患者作为研究对象,按随机数表法分为观察组(COM-B模型的护理干预)及对照组(常规护理干预),每组40例患者。分析两组患者家庭支持(PSS-Fa)、照护依赖(CDS-R)、焦虑状态(SAS)和抑郁状态(SDS)及肢体功能情况。结果:观察组患者PSS-Fa、CDS-R水平较对照组高(P<0.05);干预后,两组患者SAS、SDS评分均有所下降,且观察组患者SAS、SDS评分下降幅度高于对照组(P<0.05);干预后,观察组患者上、下肢评分优于对照组(P<0.05)。结论:将COM-B模型护理干预应用于中青年脑卒中患者家庭支持、照护依赖中,可有效提高脑卒中患者家庭照顾者的照顾能力,减轻抑郁、焦虑症状,改善肢体功能。

基于GEO数据库构建APP、APBA和APBB家族的胃癌预后评估模型

目的基于淀粉样β前体蛋白(amyloid beta precursor protein,APP)家族、淀粉样β前体蛋白结合蛋白A(amyloid beta precursor protein binding family A,APBA)家族和淀粉样β前体蛋白结合蛋白B(amyloid beta precursor protein binding family B,APBB)家族构建胃癌预后评估模型。方法从基因表达综合(gene expression omnibus,GEO)数据库下载GSE62254胃癌数据集作为训练集,GSE15459作为验证集。利用Cox回归分析筛选APP家族、APBA家族和APBB家族中胃癌预后的独立危险因素;分别建立基于三家族独立预后因素的风险评分1(risk score 1,RS1)、RS1联合病理学参数的RS2、传统TNM分期的RS3;卡方检验分析RS1与胃癌患者临床病理特征的关系;利用单细胞在线分析网站,分析纳入RS1模型的基因在不同细胞亚群中的表达情况;利用CIBERSORT分析RS1对不同免疫细胞浸润的影响;利用基因集富集分析(gene set enrichment analysis,GSEA)进行通路富集分析。结果APLP2、APBB1、APBB2是胃癌患者预后的独立危险因素(P<0.05),基于三者的风险评分RS1高组患者生存期明显短于RS1低组患者。联合临床病理学参数的Cox回归分析显示,N分期、M分期、Lauren分型和RS1是胃癌患者预后的独立危险因素(P<0.05)。基于此构建的RS2(AUC=0.767)比仅基于T分期、N分期、M分期构建的RS3(AUC=0.719)预测准确率提高了4.8%。RS1和肿瘤T分期呈正相关(P<0.05),RS1高组CD4静息细胞浸润较高,激活细胞浸润较低,M2巨噬细胞浸润较高。GSEA通路分析显示,高RS1组患者富集于MAPK、MTOR和WNT等通路。结论本研究成功构建了基于APP、APBA和APBB家族的胃癌预后评估模型,该模型能够较准确地判断胃癌患者预后。

CLEC1B、DKK1、DRD4在原发性肝癌病变组织中的表达及临床意义探究

目的分析C型凝集素结构域家族1成员B(C-type lectin domain family 1 member B,CLEC1B)、分泌型蛋白Dickkopf 1(DKK1)、多巴胺受体D4(dopamine receptor D4,DRD4)在原发性肝癌(primary hepatic cancer,PHC)患者病变组织中的表达及临床意义。方法回顾性选取2022年1月~2023年1月在四川大学华西医院接受肝癌切除术且经术后病理证实为PHC的138例患者,取其癌组织与癌旁组织石蜡病理标本,分析其CLEC1B、DKK1、DRD4表达情况及和临床病理特征关联性,Pearson法分析CLEC1B、DKK1、DRD4的相关性。结果肝癌组织中CLEC1B、DRD4表达水平均低于癌旁肝组织,肝癌组织中的DKK1蛋白表达较癌旁肝组织更高(P<0.05),且3者均主要分布于细胞浆;CLEC1B低表达、DKK1高表达、DRD4低表达分别97例(70.29%)、91例(65.94%)、78例(56.52%),PHC患者的CLEC1B低表达与术前AFP水平、血管侵犯、远处转移、肿瘤出血等密切相关(P<0.05),DKK1高表达与术前AFP水平、BCLC Kinki分期、肿瘤数目、肿瘤大小密切相关(P<0.05),DRD4低表达与术前AFP水平、肿瘤数目、肿瘤大小、卫星结节、血管侵犯密切相关(P<0.05);Pearson相关分析显示,PHC患者CLEC1B、DRD4与DKK1表达水平呈负相关(r=-0.809、r=-0.774,P<0.001),CLEC1B与DRD4表达水平呈正相关(r=0.748,P<0.001)。结论CLEC1B、DRD4在PHC患者癌变组织中呈低表达,而DKK1呈高表达,且与临床病理参数有关,CLEC1B、DKK1、DRD4可能参与PHC发生发展,有一定检测意义。

血清MBL、BAFF与磷脂酶A2受体相关特发性膜性肾病患者肾功能及疗效的关系

目的探讨血清甘露聚糖结合凝集素(MBL)、肿瘤坏死因子家族B细胞活化因子(BAFF)与磷脂酶A2受体(PLA2R)相关特发性膜性肾病(IMN)患者肾功能和疗效的关系。方法选取PLA2R相关IMN患者103例作为观察组,并根据疗效将PLA2R相关IMN患者分为未缓解组(n=44)、缓解组(n=59);同期另选健康体检志愿者67例作为对照组。收集相关资料;用酶联免疫吸法检测血清MBL、BAFF、24 h尿蛋白,PA速率微板法试剂盒检测血肌酐,计算估计肾小球滤过率(eGFR)。Spearman相关法分析PLA2R相关IMN患者血清MBL、BAFF水平与肾功能指标的相关性;多因素Logistic回归分析影响PLA2R相关IMN患者疗效的因素,受试者工作特征(ROC)曲线分析血清MBL、BAFF对PLA2R相关IMN患者治疗未缓解的预测价值。结果观察组血清MBL、BAFF水平及24 h尿蛋白高于对照组,eGFR低于对照组(P均<0.05)。PLA2R相关IMN患者血清MBL、BAFF水平与eGFR呈负相关(r分别为-0.777、-0.760,P均<0.05),与24 h尿蛋白呈正相关(r分别为0.883、0.828,P均<0.05)。未缓解组白蛋白、eGFR低于缓解组,24 h尿蛋白、MBL、BAFF高于缓解组(P均<0.05),两组性别、年龄、血肌酐、血尿酸、血尿素氮、总胆固醇、甘油三酯、IgG、补体3和PLA2R抗体滴度比较差异无统计学意义(P均>0.05)。白蛋白和eGFR升高为PLA2R相关IMN患者疗效的独立保护因素(P均<0.05),24 h尿蛋白、MBL和BAFF升高为独立危险因素(P均<0.05)。ROC曲线分析结果显示,血清MBL、BAFF联合预测PLA2R相关IMN患者治疗未缓解的曲线下面积(0.880)大于二者单独预测(0.791、0.787),比较差异有统计学意义(Z分别为2.694、2.613,P均<0.05)。结论血清MBL、BAFF水平升高与PLA2R相关IMN患者肾功能降低及疗效差有关,二者联合检测预测PLA2R相关IMN患者治疗未缓解的价值较高。

非小细胞肺癌患者血清LncRNA FAM138B、miR-105-5p表达与病理特征的关系

目的探讨非小细胞肺癌(NSCLC)患者血清长链非编码RNA序列相似性家族138成员B(LncRNA FAM138B)、微小RNA-105-5p(miR-105-5p)表达与病理特征的预后的关系。方法选取2018年1月至2019年12月收治的110例NSCLC患者为NSCLC组,另选取同期60例体检健康者为对照组,采用实时荧光定量聚合酶链式反应检测血清LncRNA FAM138B、miR-105-5p表达。分析血清LncRNA FAM138B、miR-105-5p表达与NSCLC患者病理特征的关系。StarBase数据库预测LncRNA FAM138B与miR-105-5p的关系。采用Pearson相关性分析NSCLC患者血清LncRNA FAM138B与miR-105-5p表达的相关性,多因素Cox回归分析NSCLC患者预后影响因素,受试者工作特征(ROC)曲线分析血清LncRNA FAM138B、miR-105-5p表达对NSCLC患者预后的预测价值。结果与对照组比较,NSCLC组血清LncRNA FAM138B表达降低,miR-105-5p表达升高(P<0.05)。经StarBase数据库预测,LncRNA FAM138B与miR-105-5p存在互补序列。Pearson相关性分析显示,NSCLC患者血清LncRNA FAM138B与miR-105-5p表达呈负相关(r=-0.770,P<0.001)。不同分化程度、TNM分期、淋巴结转移NSCLC患者LncRNA FAM138B、miR-105-5p表达比较,差异有统计学意义(P<0.05)。110例NSCLC患者3年总生存率为56.36%(62/110)。K-M生存曲线分析显示,LncRNA FAM138B高表达组总生存率高于低表达组,miR-105-5p高表达组总生存率高于低表达组(P<0.05)。多因素Cox回归分析显示,低分化、TNM分期Ⅲ~Ⅳ期、淋巴结转移和miR-105-5p≥1.494为NSCLC患者死亡的独立危险因素,LncRNA FAM138B≥0.871为独立保护因素(P<0.05)。ROC曲线分析显示,血清LncRNA FAM138B、miR-105-5p表达单独与联合预测NSCLC患者预后的曲线下面积分别为0.783、0.779、0.905,二项联合预测的曲线下面积最大(P<0.05)。结论NSCLC患者血清LncRNA FAM138B低表达,miR-105-5p高表达,与分化程度、TNM分期、淋巴结转移和预后有关,可能成为NSCLC患者预后的辅助预测指标。

青稞BBX基因家族鉴定及其对UV-B的响应

B-box(BBX)蛋白是植物中一类重要的转录因子,在控制植物生长发育、响应环境胁迫中起着重要作用。本研究从青稞(Hordeum vulgare L.var.nudum Hook.f.)全基因组中鉴定出18个青稞BBX基因家族成员,分析了它们的理化性质、基因结构、进化关系及UV-B照射幼苗后的表达特性等。结果表明:青稞BBX基因的编码序列(CDS)长度为636~2 223 bp;分子质量为22.07~54.29 kDa。系统发育分析将18个HvnBBX分为4个亚类。共线性分析表明青稞种内共产生4对片段复制形成的基因对,种间与玉米进化关系更近;HvnBBX启动子区域包含与逆境胁迫、激素、生长发育等相关顺式作用元件。转录组测序结果显示UV-B照射后青稞叶片中有5个BBX基因(HvnBBX2/4/5/6/17)的表达量显著下调,qRT-PCR验证结果与转录组测序一致。上述结果为深入研究HvnBBX基因的抗紫外功能提供参考。

辣椒CcBBX20基因克隆和表达分析

锌指蛋白在植物生长发育和对非生物胁迫因子的响应中发挥着重要的调节作用。BBX蛋白属于锌指蛋白,CcBBX20基因是锌指蛋白家族的重要成员。为探讨CcBBX20在辣椒生长发育和响应非生物胁迫中的生物学功能,本研究利用基因克隆获得基因的编码序列;应用生物信息学分析方法预测该基因编码蛋白质的结构和功能;根据PepperHub数据库相关数据分析该基因在10种非生物胁迫处理下辣椒幼苗根和叶的表达量模式。结果表明:辣椒CcBBX20所编码的蛋白有209个氨基酸残基,相对分子质量为23.12 kD,分子式为C984H1551N285O329S15,理论等电点为5.78,是不稳定的亲水性蛋白;有2个典型的Bbox-SF结构域,没有信号肽和跨膜结构域,主要定位在细胞核中;其二级结构主要由无规则卷曲组成(占58.85%),α螺旋占比22.01%,β-转角占比3.83%。辣椒CcBBX20和马铃薯、番茄、茄子BBX20的亲缘关系最近。基因表达分析表明CcBBX20在辣椒幼苗根和叶受到不同胁迫后表达差异显著,在脱落酸、赤霉素、生长素、水杨酸、茉莉酸甲酯以及高温处理下表现为促进作用,其中高温42℃,处理1.5 h后表达量最高;在过氧化氢、甘露醇和盐处理下表现为抑制作用。因此辣椒CcBBX20基因作为BBX基因家族成员,在响应高温胁迫中发挥作用,可能会提高幼苗对高温的耐受力,可为辣椒耐高温育种目标提供候选基因。

小麦B-ARR转录因子家族基因鉴定与表达模式分析

B型反应调节因子(B-ARR,type-B authentic response regulator)家族基因是细胞分裂素信号转导的正向调节因子,在植物生长发育和抗非生物胁迫中起重要作用。然而,目前对小麦中B-ARR基因家族的研究还很少。本研究从小麦基因组中鉴定出25个B-ARR基因家族成员,利用生物信息学方法分析其理化性质、基因结构、顺式作用元件和非生物胁迫诱导的表达模式。结果表明,B-ARR蛋白都定位于细胞核中,B-ARR蛋白二级结构以α螺旋和无规则卷曲为主要结构。B-ARR基因在小麦染色体上分布不均匀,其中7号染色体上含有的B-ARR基因数量最多。此外,在B-ARR的启动子区域中鉴定出了与生长发育、激素响应以及生物和非生物胁迫相关的多种顺式作用调节元件。RT-qPCR分析表明,基因TaARRM-like9、TaARRMlike10、TaARRM-like12和TaARRM-like13在干旱、盐和低温胁迫下表达量显著上调,能够响应非生物胁迫。本研究为进一步研究B-ARR转录因子在小麦发育和非生物胁迫应答中的作用奠定了基础。

茶树ARR-B基因家族的鉴定和表达模式分析

ARR-B基因作为细胞分裂素信号转导的正向调节因子,在植物发育及抗逆过程中发挥着重要作用。本研究基于茶树基因组数据库,利用生物信息学软件成功鉴定出23个茶树ARR-B基因,并对其基因结构、进化关系、保守结构域、染色体定位、启动子顺式作用元件以及八大组织和四种胁迫中的转录组数据等进行分析,并基于以上数据对福鼎大白茶树进行盐胁迫处理后利用荧光定量PCR技术验证表达水平。结果表明,茶树ARR-B基因家族有23个成员且在不同染色体上分布不均;根据系统进化树将茶树ARR-B基因分为9类且与拟南芥ARR-B基因同源性很高;茶树ARR-B基因相同亚家族的基因结构和保守结构域基本一致;茶树ARR-B基因在茎和根组织中具有较高的表达水平及在干旱、低温、盐和茉莉酸甲酯胁迫处理下存在差异表达;实时荧光定量PCR结果可知,经盐胁迫处理后,8个茶树ARR-B基因表达水平与转录组数据存在差异。本研究初步分析了茶树ARR-B基因家族的功能和抗逆作用,为进一步研究茶树的生长发育提供参考和依据。

尼泊尔黄堇Aux/IAA基因家族的鉴定与UVB处理下表达模式分析

【目的】鉴定尼泊尔黄堇Aux/IAA基因家族,为尼泊尔黄堇Aux/IAA基因功能研究及抵御逆境胁迫提供理论基础。【方法】基于尼泊尔黄堇全长转录组数据,鉴定和分析尼泊尔黄堇Aux/IAA(ChAux-IAA)基因家族成员,并对其蛋白质理化性质、系统进化树、基因结构、蛋白质三级结构及蛋白质互作关系、启动子顺式作用元件进行分析。此外,对尼泊尔黄堇进行不同时间(0.5 h、48 h)UV-B处理,检测ChAux/IAA基因家族的表达模式。【结果】尼泊尔黄堇ChAux/IAA基因家族共有20个成员,大多数位于细胞核中,少数位于叶绿体、线粒体和细胞壁中。ChAux/IAA基因家族编码蛋白属于亲水性蛋白。ChAux/IAA基因家族的顺式作用元件中存在光响应元件,表明该基因家族可能参与植物的生长发育和抵御紫外胁迫。通过RT-qPCR分析发现,在UV-B处理下,20个ChAux/IAA的表达量均较对照组增加,其中ChIAA2、ChIAA3、ChIAA4、ChIAA10、ChIAA13和ChIAA21表达量增加更加显著,表明ChAux/IAA基因家族在其抵御紫外线胁迫方面具有重要功能。【结论】共鉴定出20个ChAux/IAA基因家族成员,ChAux/IAA基因家族在UV-B胁迫中发挥重要作用。

基于生物信息学筛查CLEC3B蛋白及其在脓毒症诊断中的作用

目的:研究正常人与脓毒症患者血清差异表达蛋白中C-型凝集素域家族3成员B(CLEC3B)蛋白,探讨目标CLEC3B蛋白作为脓毒症分子标志物的可能性。方法:收集10名健康志愿者(健康组)和18例脓毒症患者(脓毒症组)外周血,利用数据独立采集法对外周血清蛋白数据进行采集,数据上传至i DEP在线平台分析脓毒症患者外周血中差异表达蛋白。并对这些差异蛋白进行生物信息学分析,筛选出脓毒症关键蛋白。酶联免疫吸附法(ELISA)验证并绘制关键蛋白受试者工作特征(ROC)曲线。结果:蛋白质组学分析筛选出138个差异表达蛋白(DEPs),其中34个蛋白显著下调,104个蛋白显著上调。DEPs主要富集在细胞过程、生物调节、生物过程调节、参与绑定、催化活化、分子功能监管、免疫系统、信号转导等。DEPs构建蛋白-蛋白相互作用网络(PPI)筛选出感兴趣的关键蛋白CLEC3B。ELISA实验表明脓毒症组患者CLEC3B蛋白浓度(297.73±22.00)ng/ml显著低于健康组(452.42±191.72)ng/ml,差异具有统计学意义(t=13.13,P=0.000)。CLEC3B蛋白的ROC曲线下面积为0.998,灵敏度为97.73%,特异度为100.0%。结论:CLEC3B蛋白在脓毒症组中显著降低,其灵敏度高,特异度高,可以作为脓毒症潜在的生物学诊断标志物。临床试验注册号中国临床试验注册中心,ChiCTR1900021261。