Resistivity measurement of conductive asphalt concrete based on two-electrode method

The objective of this work is to develop a novel methodology for determining real resistivity of conductive asphalt concrete based on two-electrode method.Due to an influence of contact resistance,the measured resistivity is always not equal to the real resistivity.To determine the real resistivity,a linear relationship of the measured resistivity,contact resistance and the real resistivity was established.Then experiments for six specimens with varying graphite contents were designed and performed to validate the formulation.Results of experiments demonstrate that the slope of the line represents contact resistance,and the intercept indicates the real resistivity.The effects of graphite content on contact resistance and real resistivity are also revealed.Finally,results show that the influence of contact resistance on accuracy of resisitvity measurement becomes more serious if graphite content is beyond 3%.Hence,it is the time to choose this novel methodology to determine the real resistivity of asphalt concrete by taking account of contact resistance.

Deglycosylation altered the gating properties of rNav1.3:glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel

Objective To examine the effect of deglycosylation on gating properties of rNav1.3. Methods rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control. Results Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV. Conclusion Glycosylation altered the gating properties of rNav 1.3 and contributed to the functional diversity.

Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum

Pea aphid,Acyrthosiphon pisum,is a serious pest of many different leguminous plants,and it mainly relies on its odorant receptors(Ors)to discriminate among host species.However,less is known about the role that Ors play in the host plant location.In this study,we identified a novel conserved odorant receptor clade by phylogenetic analysis,and conducted the functional analysis of ApisOr23 in A.pisum.The results showed that the homologous Ors from A.pisum,Aphis glycines and Aphis gossypii share 94.28% identity in amino acid sequences.Moreover,conserved motifs were analyzed using the annotated homologous Or23 from eight aphid species,providing further proof of the high conservation level of the Or23 clade.According to the tissue expression pattern analysis,ApisOr23 was mainly expressed in the antennae.Further functional study using a heterologous Xenopus expression system revealed that ApisOr23 was tuned to five plant volatiles,namely trans-2-hexen-1-al,cis-2-hexen-1-ol,1-heptanol,4’-ethylacetophenone,and hexyl acetate.Among them,trans-2-hexen-1-al,which is one of the main volatile organic compounds released from legume plants,activated the highest response of ApisOr23.Our findings suggest that the conserved Or23 clade in most aphid species might play an important role in host plant detection.

Mifepristone modulates serotonin transporter function

Regulating serotonin expression can be used to treat psychotic depression. Mifepristone, a glu- cocorticoid receptor antagonist, is an effective candidate for psychotic depression treatment. However, the underlying mechanism related to serotonin transporter expression is poorly un- derstood. In this study, we cloned the human brain serotonin transporter into Xenopus oocytes, to establish an in vitro expression system. Two-electrode voltage clamp recordings were used to detect serotonin transporter activity. Our results show that mifepristone attenuates serotonin transporter activity by directly inhibiting the serotonin transporter, and suggests that the se- rotonin transporter is a pharmacological target of mifepristone for the treatment of psychotic depression.

Experimental characteristics of a two-electrode plasma synthetic jet actuator array in serial

Plasma Synthetic Jet(PSJ) actuators have shown wide and promising application prospects in high-speed flow control, due to their advantages including high exhaust speed, wide frequency band, rapid response, and non-moving components. Although previous studies on PSJ actuators are abundant, most of them have focused on the performance of a single actuator. However, in practice, an actuator array is very necessary for large-scale aerodynamic actuation on account of the small affected area of a single PSJ. In this paper, the characteristics of a twoelectrode plasma synthetic jet actuator array in serial are investigated experimentally. Compared to a parallel actuator array, the serial actuator array requires simpler power supply design and is much easier to realize. High-speed photography of the discharge evolution, voltage-current measurement, and shadowgraphy visualization are used in the investigation. Experimental results show that, for the serial actuator array, weak discharges happen firstly between energized and suspending electrodes, and then a strong pulse arc discharge is triggered. The breakdown voltage in serial is irrelevant to such factors as the number of actuators, the maximum or minimum gap in serial,the connection sequence, etc. It is mainly determined by the sum of gaps. For serial actuators with the same anode-to-cathode spacing, the energy deposition is the same, and the jet is synchronous and similar. Because of the entrainment and merging of adjacent jet vortices, the jet front speed of an aligned synchronous jet array increases as the orifice distance decreases. To achieve the highest jet front velocity, the orifice of the actuator has an optimal diameter.

Electrochemical quartz crystal microbalance study on the two-electrode-system cyclic voltammetric behavior of Prussian blue films

A two-channel electrochemical quartz crystal microbalance (EQCM) was used to investigate the cyclic voltammetric behavior of two Prussian blue (PB) film-modified Au electrodes in a two-electrode con-figuration in aqueous solution. The redox peaks observed in the two-electrode cyclic voltammogram (CV) are assigned to the intrinsic redox transitions among the Everitt's salt, PB, and Prussian yellow for the film itself, the redox process of the Au substrate and the redox process of small-quantity ferri-/ferrocyanide impurities entrapped in the PB film, as also supported by ultraviolet-visible (UV-Vis) spectroelectrochemical data. The profile of the two-electrode solid-state CV for the PB powder sand-wiched between two gold-coated indium-tin oxide (ITO) electrodes is similar to that for two PB-modified Au electrodes in aqueous solution, implying similar origins for the corresponding redox peaks. The two-channel EQCM method is expected to become a highly effective technique for the studies of the two-electrode electrochemical behaviors of many other species/materials.

Neural Firing Mechanism Underlying Two-Electrode Discrimination by 3D Transcutaneous Electrical Nerve Stimulation Computational Model

Transcutaneous electrical nerve stimulation(TENS) has been widely used for sensory feedback which is a key consideration of improving the performance of prosthetic hands. Two-electrode discriminability is the key to realize high-spatial-resolution TENS, but the neural firing mechanism is not clear yet. The goal of this research is to investigate the neural firing patterns under two-electrode stimulation and to reveal the potential mechanisms. A three-dimensional(3 D) model is established by incorporating Aβ fiber neuron clusters into a layered forearm structure. The diameters of the stimulating electrodes are selected as 5, 7, 9 and 12 mm, and the two-electrode discrimination distance(TEDD) is quantified. It is found that a distant TEDD is obtained for a relatively large electrode size, and 7 mm is suggested to be the optimal diameter of stimulating electrodes. The present study reveals the neural firing patterns under two-electrode stimulation by the 3 D TENS model. In order to discriminate individual electrodes under simultaneous stimulation, no crosstalk of activated Aβ fibers exists between two electrodes. This research can further guide the optimization of the electrode-array floorplan.

Moth sex pheromones affect interspecific competition among sympatric species and possibly population distribution by modulating pre-mating behavior

Premating behaviors mediated by pheromones play pivotal roles in animal mating choices.In natural populations of the striped stem borer Chilo suppressalis and the rice leaf roller Cnaphalocrocis medinalis in the rice field habitat,we discovered that Z11-16:Ald,a major component of the C.suppressalis pheromone,modulated the premating behavior of C.medinalis.Z11-16:Ald evoked a strong olfactory response in male antennae and strongly inhibited the sex pheromone trapping of male C.medinalis in the field.The functions of three C.medinalis sex pheromone receptor genes(CmedPR1–3)were verified through heterologous expression in Xenopus oocytes.CmedPR1 responded to Z11-18:OH and Z11-18:Ald,as well as the interspecific pheromone compound Z11-16:Ac of sympatric species;CmedPR2 responded to Z13-18:OH and Z13-18:Ald,as well as the sex pheromone compounds Z11-16:Ald and Z9-16:Ald of sympatric species;and CmedPR3 responded to Z11-18:OH and Z13-18:OH,as well as the interspecific pheromones Z11-16:OH,Z9-16:Ald,Z11-16:Ac,and Z11-16:Ald of sympatric species.Thus,CmedPR2 and CmedPR3 share the ligand Z11-16:Ald,which is not a component of the C.medinalis sex pheromone.Therefore,the sex pheromones of interspecific species affected the input of neural signals by stimulating the sex pheromone receptors on the antennae of male C.medinalis moths,thereby inhibiting the olfactory responses of the male moths to the sex pheromones.Our results demonstrate chemical communication among sympatric species in the rice field habitat,the recognition of intra-and interspecific sex pheromones by olfactory receptors,and how insect premating behaviors are modulated to possibly affect resource partitioning.